CN1845989B - 抗微生物剂的重组产生 - Google Patents

抗微生物剂的重组产生 Download PDF

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Publication number
CN1845989B
CN1845989B CN2004800249767A CN200480024976A CN1845989B CN 1845989 B CN1845989 B CN 1845989B CN 2004800249767 A CN2004800249767 A CN 2004800249767A CN 200480024976 A CN200480024976 A CN 200480024976A CN 1845989 B CN1845989 B CN 1845989B
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China
Prior art keywords
peptide
gly
ala
ser
thr
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Expired - Fee Related
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CN2004800249767A
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English (en)
Chinese (zh)
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CN1845989A (zh
Inventor
E·B·詹森
H-H·K·霍根豪格
P·K·汉森
P·E·彼得森
P·H·米京德
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Novozymes Biopharma DK AS
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Novozymes Adenium Biotech AS
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Publication of CN1845989A publication Critical patent/CN1845989A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Polymers & Plastics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CN2004800249767A 2003-09-11 2004-09-13 抗微生物剂的重组产生 Expired - Fee Related CN1845989B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200301310 2003-09-11
DKPA200301310 2003-09-11
PCT/DK2004/000605 WO2005024002A1 (en) 2003-09-11 2004-09-13 Recombinant production of antimicrobial agents

Publications (2)

Publication Number Publication Date
CN1845989A CN1845989A (zh) 2006-10-11
CN1845989B true CN1845989B (zh) 2011-10-12

Family

ID=34259082

Family Applications (1)

Application Number Title Priority Date Filing Date
CN2004800249767A Expired - Fee Related CN1845989B (zh) 2003-09-11 2004-09-13 抗微生物剂的重组产生

Country Status (14)

Country Link
US (1) US7935501B2 (enExample)
EP (1) EP1680503B1 (enExample)
JP (1) JP2007504810A (enExample)
CN (1) CN1845989B (enExample)
AT (1) ATE513904T1 (enExample)
AU (1) AU2004270815B2 (enExample)
BR (1) BRPI0413833A (enExample)
CA (1) CA2536782A1 (enExample)
DK (1) DK1680503T3 (enExample)
ES (1) ES2368221T3 (enExample)
IL (1) IL173656A0 (enExample)
NZ (1) NZ545564A (enExample)
WO (1) WO2005024002A1 (enExample)
ZA (1) ZA200601544B (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6855548B2 (en) * 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
US7588926B2 (en) * 2003-02-07 2009-09-15 Novozymes A/S Proteases
US7763706B1 (en) * 2003-07-17 2010-07-27 Campina B.V. Arginine/lysine-enriched peptides
JP2007509603A (ja) 2003-10-10 2007-04-19 ノボザイムス アクティーゼルスカブ プロテアーゼ変異型
US8357408B2 (en) * 2004-06-21 2013-01-22 Novozymes A/S Proteases
GB0815484D0 (en) 2008-08-26 2008-10-01 Univ Leuven Kath Antibacterial agents
EP2387390B1 (en) * 2009-01-13 2012-11-28 PharmaSurgics in Sweden AB Hylauronic acid containing compositions for treatment of wounds, scars, post-surgical adhesion formation
SG177704A1 (en) 2009-06-26 2012-02-28 Lysando Holding Ag Antimicrobial agents
ES2579806T3 (es) * 2009-06-26 2016-08-16 Katholieke Universiteit Leuven, K.U. Leuven R&D Agentes antimicrobianos
CN102220295B (zh) * 2011-05-26 2013-02-06 长沙理工大学 以稻谷加工副产物为原料生产饲用葡萄糖氧化酶的方法
IN2014CN04408A (enExample) * 2011-11-17 2015-09-04 Dsm Ip Assets Bv
US10597430B2 (en) * 2012-03-05 2020-03-24 Instituto De Investigaciones Agropecuarias Chimeric gene for heterologous expression which encodes for peptides with antimicrobial activity
WO2014052438A1 (en) 2012-09-25 2014-04-03 Regents Of The University Of Minnesota Methods for making and using antimicrobial peptides
CN105734029B (zh) * 2014-12-12 2020-09-25 丰益(上海)生物技术研发中心有限公司 磷脂酶抗菌肽
CN105255939B (zh) * 2015-11-13 2018-12-25 长沙中科晶博生物科技有限公司 一种用青蒿生产菌丝霉素的方法
CN105274137B (zh) * 2015-11-13 2018-12-25 长沙中科晶博生物科技有限公司 一种用青蒿生产乳铁蛋白的方法
CN108794635B (zh) * 2018-04-04 2021-03-19 浙江工业大学 一种牛乳铁蛋白肽-人溶菌酶融合蛋白、基因及其应用
CN111118091A (zh) * 2018-10-31 2020-05-08 宁夏乙征生物工程有限公司 一种抗菌肽的生产方法
CN109295075B (zh) * 2018-10-31 2021-04-20 上海市农业生物基因中心 NfOCP1抗旱基因、其编码的氨基酸序列及其在提高植物抗旱性中的用途
CN111349177B (zh) * 2020-03-13 2022-04-22 西北农林科技大学 一种融合抗菌肽cat的制备方法和应用
CN114478795B (zh) * 2020-11-13 2024-06-04 安徽新熙盟生物科技有限公司 提高多肽类药物口服给药稳定性的融合蛋白及其应用
CN113125689B (zh) * 2021-03-29 2022-02-22 创芯国际生物科技(广州)有限公司 一种新型mtt细胞活力检测试剂盒及其应用
AU2023272468A1 (en) 2022-05-14 2024-11-14 Novonesis Plant Biosolutions A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN114790237B (zh) * 2022-05-31 2024-04-05 合肥工业大学 一种抗菌肽及其应用
CN118064343B (zh) * 2024-03-11 2025-01-03 华南农业大学 一种产四种抗菌肽的重组枯草芽孢杆菌、构建方法及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5094951A (en) 1988-06-21 1992-03-10 Chiron Corporation Production of glucose oxidase in recombinant systems
US5571697A (en) * 1989-05-05 1996-11-05 Baylor College Of Medicine Texas Medical Center Expression of processed recombinant lactoferrin and lactoferrin polypeptide fragments from a fusion product in Aspergillus
US5789377A (en) * 1992-08-21 1998-08-04 University Of British Columbia Treatment of endotoxin-associated disorders with cationic peptides
KR100263583B1 (ko) 1997-05-28 2000-08-01 박종헌 항균 펩타이드의 대량 제조방법 및 그에 유용한 플라즈미드 벡타
WO2000075344A1 (en) 1999-06-02 2000-12-14 Novozymes A/S Pectate lyase fusion for expression and secretion of polypeptides

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
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OKAMOTO MASAJI 等.EXPRESSION OF AN ANTIMICROBIAL PEPTIDESARCOTOXIN IA BY GUS FUSION IN TRANSGENICTOBACCO PLANTS.PLANT AND CELL PHYSIOLOGY39 1.1998,39(1),57-63.
OKAMOTO MASAJI 等.EXPRESSION OF AN ANTIMICROBIAL PEPTIDESARCOTOXIN IA BY GUS FUSION IN TRANSGENICTOBACCO PLANTS.PLANT AND CELL PHYSIOLOGY39 1.1998,39(1),57-63. *

Also Published As

Publication number Publication date
EP1680503A1 (en) 2006-07-19
ZA200601544B (en) 2007-04-25
CA2536782A1 (en) 2005-03-17
CN1845989A (zh) 2006-10-11
WO2005024002A1 (en) 2005-03-17
NZ545564A (en) 2008-07-31
IL173656A0 (en) 2006-07-05
ES2368221T3 (es) 2011-11-15
EP1680503B1 (en) 2011-06-22
JP2007504810A (ja) 2007-03-08
DK1680503T3 (da) 2011-10-03
ATE513904T1 (de) 2011-07-15
AU2004270815A1 (en) 2005-03-17
BRPI0413833A (pt) 2006-10-24
US20070104764A1 (en) 2007-05-10
AU2004270815B2 (en) 2011-06-02
US7935501B2 (en) 2011-05-03

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