JP2006506084A5 - - Google Patents

Info

Publication number

JP2006506084A5

JP2006506084A5 JP2004552811A JP2004552811A JP2006506084A5 JP 2006506084 A5 JP2006506084 A5 JP 2006506084A5 JP 2004552811 A JP2004552811 A JP 2004552811A JP 2004552811 A JP2004552811 A JP 2004552811A JP 2006506084 A5 JP2006506084 A5 JP 2006506084A5

Authority

JP

Japan

Prior art keywords

seq

polynucleotide

protein

sequence

cells

Prior art date

2003-11-18

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 108091033319 polynucleotide Proteins 0.000 claims 41
• 102000040430 polynucleotide Human genes 0.000 claims 41
• 239000002157 polynucleotide Substances 0.000 claims 41
• 108090000623 proteins and genes Proteins 0.000 claims 35
• 102000004169 proteins and genes Human genes 0.000 claims 28
• 210000004027 cell Anatomy 0.000 claims 24
• 238000000034 method Methods 0.000 claims 13
• 239000012634 fragment Substances 0.000 claims 11
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 10
• 239000000126 substance Substances 0.000 claims 9
• 239000012472 biological sample Substances 0.000 claims 8
• 150000001875 compounds Chemical class 0.000 claims 8
• 102000039446 nucleic acids Human genes 0.000 claims 8
• 108020004707 nucleic acids Proteins 0.000 claims 8
• 150000007523 nucleic acids Chemical class 0.000 claims 8
• 239000000523 sample Substances 0.000 claims 8
• 230000009261 transgenic effect Effects 0.000 claims 8
• 210000000227 basophil cell of anterior lobe of hypophysis Anatomy 0.000 claims 6
• 238000000018 DNA microarray Methods 0.000 claims 5
• 230000003321 amplification Effects 0.000 claims 5
• 230000014509 gene expression Effects 0.000 claims 5
• 210000004153 islets of langerhan Anatomy 0.000 claims 5
• 238000003199 nucleic acid amplification method Methods 0.000 claims 5
• 239000003153 chemical reaction reagent Substances 0.000 claims 4
• 230000004069 differentiation Effects 0.000 claims 4
• 230000035772 mutation Effects 0.000 claims 4
• 239000002773 nucleotide Substances 0.000 claims 4
• 125000003729 nucleotide group Chemical group 0.000 claims 4
• 229920001184 polypeptide Polymers 0.000 claims 4
• 102000004196 processed proteins & peptides Human genes 0.000 claims 4
• 108090000765 processed proteins & peptides Proteins 0.000 claims 4
• 210000000130 stem cell Anatomy 0.000 claims 4
• 239000013598 vector Substances 0.000 claims 4
• 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 3
• 108020004459 Small interfering RNA Proteins 0.000 claims 3
• 206010012601 diabetes mellitus Diseases 0.000 claims 3
• 238000009396 hybridization Methods 0.000 claims 3
• 201000008980 hyperinsulinism Diseases 0.000 claims 3
• 238000000338 in vitro Methods 0.000 claims 3
• 230000028327 secretion Effects 0.000 claims 3
• 239000004055 small Interfering RNA Substances 0.000 claims 3
• 238000013518 transcription Methods 0.000 claims 3
• 230000035897 transcription Effects 0.000 claims 3
• 108090001061 Insulin Proteins 0.000 claims 2
• 230000002159 abnormal effect Effects 0.000 claims 2
• 150000001413 amino acids Chemical class 0.000 claims 2
• 230000000692 anti-sense effect Effects 0.000 claims 2
• 230000015556 catabolic process Effects 0.000 claims 2
• 230000008859 change Effects 0.000 claims 2
• 238000006243 chemical reaction Methods 0.000 claims 2
• 230000000295 complement effect Effects 0.000 claims 2
• 238000006731 degradation reaction Methods 0.000 claims 2
• 239000003814 drug Substances 0.000 claims 2
• 238000001727 in vivo Methods 0.000 claims 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
• 230000003993 interaction Effects 0.000 claims 2
• 238000004519 manufacturing process Methods 0.000 claims 2
• 230000035800 maturation Effects 0.000 claims 2
• 108020004999 messenger RNA Proteins 0.000 claims 2
• 230000002265 prevention Effects 0.000 claims 2
• 230000004044 response Effects 0.000 claims 2
• 238000012216 screening Methods 0.000 claims 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 1
• 241000282412 Homo Species 0.000 claims 1
• 102000004877 Insulin Human genes 0.000 claims 1
• HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims 1
• 230000005856 abnormality Effects 0.000 claims 1
• 230000001640 apoptogenic effect Effects 0.000 claims 1
• 230000006907 apoptotic process Effects 0.000 claims 1
• 238000003556 assay Methods 0.000 claims 1
• 230000030833 cell death Effects 0.000 claims 1
• 238000010367 cloning Methods 0.000 claims 1
• 239000013599 cloning vector Substances 0.000 claims 1
• 238000012217 deletion Methods 0.000 claims 1
• 230000037430 deletion Effects 0.000 claims 1
• 230000000667 effect on insulin Effects 0.000 claims 1
• 230000001747 exhibiting effect Effects 0.000 claims 1
• 239000013604 expression vector Substances 0.000 claims 1
• 239000008103 glucose Substances 0.000 claims 1
• 229940125396 insulin Drugs 0.000 claims 1
• 230000003914 insulin secretion Effects 0.000 claims 1
• 210000002660 insulin-secreting cell Anatomy 0.000 claims 1
• 210000004962 mammalian cell Anatomy 0.000 claims 1
• 230000004048 modification Effects 0.000 claims 1
• 238000012986 modification Methods 0.000 claims 1
• 230000002018 overexpression Effects 0.000 claims 1
• 230000036542 oxidative stress Effects 0.000 claims 1
• 230000008569 process Effects 0.000 claims 1
• 238000010839 reverse transcription Methods 0.000 claims 1
• 230000035945 sensitivity Effects 0.000 claims 1
• 238000012163 sequencing technique Methods 0.000 claims 1
• 230000000638 stimulation Effects 0.000 claims 1
• 239000011701 zinc Substances 0.000 claims 1
• 229910052725 zinc Inorganic materials 0.000 claims 1