JP2006501836A5 - - Google Patents
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- Publication number
- JP2006501836A5 JP2006501836A5 JP2004542659A JP2004542659A JP2006501836A5 JP 2006501836 A5 JP2006501836 A5 JP 2006501836A5 JP 2004542659 A JP2004542659 A JP 2004542659A JP 2004542659 A JP2004542659 A JP 2004542659A JP 2006501836 A5 JP2006501836 A5 JP 2006501836A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- sequence
- binding substance
- sample
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims 35
- 238000000034 method Methods 0.000 claims 34
- 239000000126 substance Substances 0.000 claims 19
- 230000027455 binding Effects 0.000 claims 18
- 108020004414 DNA Proteins 0.000 claims 17
- 150000007523 nucleic acids Chemical group 0.000 claims 15
- 230000003321 amplification Effects 0.000 claims 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims 13
- KKZJGLLVHKMTCM-UHFFFAOYSA-N mitoxantrone Chemical group O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO KKZJGLLVHKMTCM-UHFFFAOYSA-N 0.000 claims 10
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 239000007850 fluorescent dye Substances 0.000 claims 6
- 229960001156 mitoxantrone Drugs 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 5
- 150000001875 compounds Chemical class 0.000 claims 4
- 238000012544 monitoring process Methods 0.000 claims 4
- 150000003839 salts Chemical class 0.000 claims 4
- 125000004432 carbon atom Chemical group C* 0.000 claims 3
- 125000000524 functional group Chemical group 0.000 claims 3
- 238000003752 polymerase chain reaction Methods 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 238000001228 spectrum Methods 0.000 claims 3
- 230000004568 DNA-binding Effects 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 125000002947 alkylene group Chemical group 0.000 claims 2
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 claims 2
- 150000004056 anthraquinones Chemical class 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000001506 fluorescence spectroscopy Methods 0.000 claims 2
- 125000001183 hydrocarbyl group Chemical group 0.000 claims 2
- 229910052739 hydrogen Inorganic materials 0.000 claims 2
- 239000001257 hydrogen Substances 0.000 claims 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- 125000004433 nitrogen atom Chemical group N* 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 2
- STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 claims 1
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- 125000004423 acyloxy group Chemical group 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 125000003545 alkoxy group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 229940045799 anthracyclines and related substance Drugs 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 125000004429 atom Chemical group 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 125000004122 cyclic group Chemical group 0.000 claims 1
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 claims 1
- 230000001687 destabilization Effects 0.000 claims 1
- 125000004990 dihydroxyalkyl group Chemical group 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 125000005843 halogen group Chemical group 0.000 claims 1
- 125000005842 heteroatom Chemical group 0.000 claims 1
- 125000000623 heterocyclic group Chemical group 0.000 claims 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 claims 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000001022 rhodamine dye Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0223563.8A GB0223563D0 (en) | 2002-10-10 | 2002-10-10 | Detection system |
| PCT/GB2003/004412 WO2004033726A1 (en) | 2002-10-10 | 2003-10-10 | Detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006501836A JP2006501836A (ja) | 2006-01-19 |
| JP2006501836A5 true JP2006501836A5 (https=) | 2006-09-14 |
Family
ID=9945669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004542659A Pending JP2006501836A (ja) | 2002-10-10 | 2003-10-10 | 検出システム |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20060127906A1 (https=) |
| EP (1) | EP1554404B1 (https=) |
| JP (1) | JP2006501836A (https=) |
| CN (1) | CN1723288A (https=) |
| AT (1) | ATE429513T1 (https=) |
| AU (1) | AU2003274316A1 (https=) |
| CA (1) | CA2501636A1 (https=) |
| DE (1) | DE60327345D1 (https=) |
| GB (1) | GB0223563D0 (https=) |
| WO (1) | WO2004033726A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| JP4851188B2 (ja) | 2003-12-19 | 2012-01-11 | 日鉄環境エンジニアリング株式会社 | 核酸測定用新規混合物、及びそれを用いる核酸の新規測定方法並びにそれらに用いる核酸プローブ |
| US20070072211A1 (en) | 2005-06-30 | 2007-03-29 | Roche Molecular Systems, Inc. | Asymmetric PCR coupled with post-PCR characterization for the identification of nucleic acids |
| GB0603190D0 (en) * | 2006-02-16 | 2006-03-29 | Enigma Diagnostics Ltd | Detection system |
| US8133984B2 (en) | 2006-03-16 | 2012-03-13 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
| US8658366B2 (en) * | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| GB201004339D0 (en) | 2010-03-16 | 2010-04-28 | Enigma Diagnostics Ltd | Sequence detection assay |
| GB201007868D0 (en) | 2010-05-11 | 2010-06-23 | Enigma Diagnostics Ltd | Sequence detection assay |
| US20140186827A1 (en) | 2010-05-14 | 2014-07-03 | Fluidigm, Inc. | Assays for the detection of genotype, mutations, and/or aneuploidy |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| JP6702951B2 (ja) * | 2014-09-26 | 2020-06-03 | オンテラ インコーポレイテッド | 合成プローブのナノ細孔センシングによる標的配列検出 |
| US11486873B2 (en) | 2016-03-31 | 2022-11-01 | Ontera Inc. | Multipore determination of fractional abundance of polynucleotide sequences in a sample |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| DE102019219531A1 (de) * | 2019-12-13 | 2021-06-17 | Robert Bosch Gmbh | Verfahren zur Durchführung einer Amplifikationsreaktion in einer mikrofluidischen Vorrichtung |
| CN111562244B (zh) * | 2020-05-25 | 2023-02-07 | 赣南师范大学 | 一种检测柔红霉素的稀土时间分辨荧光探针和试剂盒 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1533151A (fr) * | 1962-05-18 | 1968-07-19 | Rhone Poulenc Sa | Nouvel antibiotique et sa préparation |
| US4012284A (en) * | 1962-11-16 | 1977-03-15 | Societa' Farmaceutici Italia, S.p.A. | Process of preparation of antibiotic F.I. 1762 derivatives |
| US3183157A (en) * | 1963-02-01 | 1965-05-11 | Upjohn Co | Antibiotic nogalamycin and method of producing |
| US4197249A (en) * | 1977-08-15 | 1980-04-08 | American Cyanamid Company | 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| GB8923075D0 (en) * | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| AU689815B2 (en) * | 1993-08-30 | 1998-04-09 | Promega Corporation | Nucleic acid purification compositions and methods |
| US5491063A (en) * | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| DE69527125T2 (de) * | 1994-11-09 | 2003-02-27 | Hitachi, Ltd. | Verfahren und Vorrichtung zur DNS Analyse |
| US5858397A (en) * | 1995-10-11 | 1999-01-12 | University Of British Columbia | Liposomal formulations of mitoxantrone |
| EP1736554B1 (en) * | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| CN1251609A (zh) * | 1997-02-12 | 2000-04-26 | 尤金·Y·查恩 | 分析聚合物的方法和产品 |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| US6646149B1 (en) * | 1997-07-15 | 2003-11-11 | Nicolaas M. J. Vermeulin | Polyamine analogues as therapeutic and diagnostic agents |
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| KR100386606B1 (ko) * | 2001-02-03 | 2003-06-02 | 엘지전자 주식회사 | Ｄｎａ 검출 방법 및 그 장치 |
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| KR100459394B1 (ko) * | 2001-10-30 | 2004-12-03 | 엘지전자 주식회사 | 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법 |
| AU2002351193A1 (en) * | 2001-11-28 | 2003-06-10 | Mj Bioworks Incorporated | Parallel polymorphism scoring by amplification and error correction |
| ATE414177T1 (de) * | 2002-09-30 | 2008-11-15 | Hoffmann La Roche | Oligonukleotide zur genotypisierung des thymidylat-synthase gens |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| EP1694869A2 (en) * | 2003-11-10 | 2006-08-30 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2002
- 2002-10-10 GB GBGB0223563.8A patent/GB0223563D0/en not_active Ceased
-
2003
- 2003-10-10 AT AT03758305T patent/ATE429513T1/de not_active IP Right Cessation
- 2003-10-10 US US10/530,980 patent/US20060127906A1/en not_active Abandoned
- 2003-10-10 CA CA002501636A patent/CA2501636A1/en not_active Abandoned
- 2003-10-10 JP JP2004542659A patent/JP2006501836A/ja active Pending
- 2003-10-10 CN CNA2003801056004A patent/CN1723288A/zh active Pending
- 2003-10-10 AU AU2003274316A patent/AU2003274316A1/en not_active Abandoned
- 2003-10-10 DE DE60327345T patent/DE60327345D1/de not_active Expired - Lifetime
- 2003-10-10 EP EP03758305A patent/EP1554404B1/en not_active Expired - Lifetime
- 2003-10-10 WO PCT/GB2003/004412 patent/WO2004033726A1/en not_active Ceased
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