JP2006501836A5 - - Google Patents
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- Publication number
- JP2006501836A5 JP2006501836A5 JP2004542659A JP2004542659A JP2006501836A5 JP 2006501836 A5 JP2006501836 A5 JP 2006501836A5 JP 2004542659 A JP2004542659 A JP 2004542659A JP 2004542659 A JP2004542659 A JP 2004542659A JP 2006501836 A5 JP2006501836 A5 JP 2006501836A5
- Authority
- JP
- Japan
- Prior art keywords
- probe
- sequence
- binding substance
- sample
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000523 sample Substances 0.000 claims 35
- 238000000034 method Methods 0.000 claims 34
- 239000000126 substance Substances 0.000 claims 19
- 230000027455 binding Effects 0.000 claims 18
- 108020004414 DNA Proteins 0.000 claims 17
- 150000007523 nucleic acids Chemical group 0.000 claims 15
- 230000003321 amplification Effects 0.000 claims 13
- 238000003199 nucleic acid amplification method Methods 0.000 claims 13
- KKZJGLLVHKMTCM-UHFFFAOYSA-N mitoxantrone Chemical group O=C1C2=C(O)C=CC(O)=C2C(=O)C2=C1C(NCCNCCO)=CC=C2NCCNCCO KKZJGLLVHKMTCM-UHFFFAOYSA-N 0.000 claims 10
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 6
- 239000007850 fluorescent dye Substances 0.000 claims 6
- 229960001156 mitoxantrone Drugs 0.000 claims 6
- 238000009396 hybridization Methods 0.000 claims 5
- 150000001875 compounds Chemical class 0.000 claims 4
- 238000012544 monitoring process Methods 0.000 claims 4
- 150000003839 salts Chemical class 0.000 claims 4
- 125000004432 carbon atom Chemical group C* 0.000 claims 3
- 125000000524 functional group Chemical group 0.000 claims 3
- 238000003752 polymerase chain reaction Methods 0.000 claims 3
- 108091033319 polynucleotide Proteins 0.000 claims 3
- 102000040430 polynucleotide Human genes 0.000 claims 3
- 239000002157 polynucleotide Substances 0.000 claims 3
- 238000001228 spectrum Methods 0.000 claims 3
- 230000004568 DNA-binding Effects 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 125000002947 alkylene group Chemical group 0.000 claims 2
- PYKYMHQGRFAEBM-UHFFFAOYSA-N anthraquinone Natural products CCC(=O)c1c(O)c2C(=O)C3C(C=CC=C3O)C(=O)c2cc1CC(=O)OC PYKYMHQGRFAEBM-UHFFFAOYSA-N 0.000 claims 2
- 150000004056 anthraquinones Chemical class 0.000 claims 2
- 229910052799 carbon Inorganic materials 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 claims 2
- 238000001506 fluorescence spectroscopy Methods 0.000 claims 2
- 125000001183 hydrocarbyl group Chemical group 0.000 claims 2
- 229910052739 hydrogen Inorganic materials 0.000 claims 2
- 239000001257 hydrogen Substances 0.000 claims 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 2
- 239000000463 material Substances 0.000 claims 2
- 239000000203 mixture Substances 0.000 claims 2
- 229910052757 nitrogen Inorganic materials 0.000 claims 2
- 125000004433 nitrogen atom Chemical group N* 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 2
- STQGQHZAVUOBTE-UHFFFAOYSA-N 7-Cyan-hept-2t-en-4,6-diinsaeure Natural products C1=2C(O)=C3C(=O)C=4C(OC)=CC=CC=4C(=O)C3=C(O)C=2CC(O)(C(C)=O)CC1OC1CC(N)C(O)C(C)O1 STQGQHZAVUOBTE-UHFFFAOYSA-N 0.000 claims 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 1
- WEAHRLBPCANXCN-UHFFFAOYSA-N Daunomycin Natural products CCC1(O)CC(OC2CC(N)C(O)C(C)O2)c3cc4C(=O)c5c(OC)cccc5C(=O)c4c(O)c3C1 WEAHRLBPCANXCN-UHFFFAOYSA-N 0.000 claims 1
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims 1
- 125000004423 acyloxy group Chemical group 0.000 claims 1
- 239000002671 adjuvant Substances 0.000 claims 1
- 125000003545 alkoxy group Chemical group 0.000 claims 1
- 125000000217 alkyl group Chemical group 0.000 claims 1
- 229940045799 anthracyclines and related substance Drugs 0.000 claims 1
- 125000003118 aryl group Chemical group 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 125000004429 atom Chemical group 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 125000004122 cyclic group Chemical group 0.000 claims 1
- STQGQHZAVUOBTE-VGBVRHCVSA-N daunorubicin Chemical compound O([C@H]1C[C@@](O)(CC=2C(O)=C3C(=O)C=4C=CC=C(C=4C(=O)C3=C(O)C=21)OC)C(C)=O)[C@H]1C[C@H](N)[C@H](O)[C@H](C)O1 STQGQHZAVUOBTE-VGBVRHCVSA-N 0.000 claims 1
- 230000001687 destabilization Effects 0.000 claims 1
- 125000004990 dihydroxyalkyl group Chemical group 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 125000005843 halogen group Chemical group 0.000 claims 1
- 125000005842 heteroatom Chemical group 0.000 claims 1
- 125000000623 heterocyclic group Chemical group 0.000 claims 1
- 125000004356 hydroxy functional group Chemical group O* 0.000 claims 1
- 125000002768 hydroxyalkyl group Chemical group 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 239000002751 oligonucleotide probe Substances 0.000 claims 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims 1
- 239000001022 rhodamine dye Substances 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0223563.8A GB0223563D0 (en) | 2002-10-10 | 2002-10-10 | Detection system |
| PCT/GB2003/004412 WO2004033726A1 (en) | 2002-10-10 | 2003-10-10 | Detection system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2006501836A JP2006501836A (ja) | 2006-01-19 |
| JP2006501836A5 true JP2006501836A5 (https=) | 2006-09-14 |
Family
ID=9945669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004542659A Pending JP2006501836A (ja) | 2002-10-10 | 2003-10-10 | 検出システム |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20060127906A1 (https=) |
| EP (1) | EP1554404B1 (https=) |
| JP (1) | JP2006501836A (https=) |
| CN (1) | CN1723288A (https=) |
| AT (1) | ATE429513T1 (https=) |
| AU (1) | AU2003274316A1 (https=) |
| CA (1) | CA2501636A1 (https=) |
| DE (1) | DE60327345D1 (https=) |
| GB (1) | GB0223563D0 (https=) |
| WO (1) | WO2004033726A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| EP1698897B1 (en) | 2003-12-19 | 2010-03-17 | Nippon Steel Kankyo Engineering Co., Ltd. | Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor |
| US7919242B2 (en) | 2005-06-30 | 2011-04-05 | Roche Molecular Systems, Inc. | Light emission modifiers and their uses in nucleic acid detection, amplification and analysis |
| GB0603190D0 (en) * | 2006-02-16 | 2006-03-29 | Enigma Diagnostics Ltd | Detection system |
| CA2645136C (en) | 2006-03-16 | 2017-09-19 | Pentabase Aps | Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids |
| JP2009044967A (ja) * | 2007-08-14 | 2009-03-05 | Sony Corp | 二本鎖核酸の形成に係る情報を得る方法 |
| US8658366B2 (en) * | 2008-09-18 | 2014-02-25 | Roche Molecular Systems, Inc. | Detection of target variants using a fluorescent label and a soluble quencher |
| GB201004339D0 (en) | 2010-03-16 | 2010-04-28 | Enigma Diagnostics Ltd | Sequence detection assay |
| GB201007868D0 (en) | 2010-05-11 | 2010-06-23 | Enigma Diagnostics Ltd | Sequence detection assay |
| EP2569447A4 (en) | 2010-05-14 | 2013-11-27 | Fluidigm Corp | ASSAYS FOR THE DETECTION OF GENOTYPES, MUTATIONS OR ANEUPLOIDY |
| AU2013202808B2 (en) | 2012-07-31 | 2014-11-13 | Gen-Probe Incorporated | System and method for performing multiplex thermal melt analysis |
| KR20170064540A (ko) * | 2014-09-26 | 2017-06-09 | 투 포어 가이즈, 인코포레이티드 | 합성 프로브의 나노포어 감지에 의한 표적 서열 검출 |
| US11486873B2 (en) | 2016-03-31 | 2022-11-01 | Ontera Inc. | Multipore determination of fractional abundance of polynucleotide sequences in a sample |
| GB201713251D0 (en) * | 2017-08-18 | 2017-10-04 | Lgc Genomics Ltd | Methods and kits for detection of nucleic acid molecules |
| DE102019219531A1 (de) * | 2019-12-13 | 2021-06-17 | Robert Bosch Gmbh | Verfahren zur Durchführung einer Amplifikationsreaktion in einer mikrofluidischen Vorrichtung |
| CN111562244B (zh) * | 2020-05-25 | 2023-02-07 | 赣南师范大学 | 一种检测柔红霉素的稀土时间分辨荧光探针和试剂盒 |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR1533151A (fr) * | 1962-05-18 | 1968-07-19 | Rhone Poulenc Sa | Nouvel antibiotique et sa préparation |
| US4012284A (en) * | 1962-11-16 | 1977-03-15 | Societa' Farmaceutici Italia, S.p.A. | Process of preparation of antibiotic F.I. 1762 derivatives |
| US3183157A (en) * | 1963-02-01 | 1965-05-11 | Upjohn Co | Antibiotic nogalamycin and method of producing |
| US4197249A (en) * | 1977-08-15 | 1980-04-08 | American Cyanamid Company | 1,4-Bis(substituted-amino)-5,8-dihydroxyanthraquinones and leuco bases thereof |
| US4469863A (en) * | 1980-11-12 | 1984-09-04 | Ts O Paul O P | Nonionic nucleic acid alkyl and aryl phosphonates and processes for manufacture and use thereof |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| US4868103A (en) * | 1986-02-19 | 1989-09-19 | Enzo Biochem, Inc. | Analyte detection by means of energy transfer |
| US5208323A (en) * | 1989-08-10 | 1993-05-04 | Universite Laval | Coupling of an anti-tumor to an antibody using glutaraldehyde preactivated anti-tumor agent |
| GB8923075D0 (en) * | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| US5994056A (en) * | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| US5567583A (en) * | 1991-12-16 | 1996-10-22 | Biotronics Corporation | Methods for reducing non-specific priming in DNA detection |
| CA2170604C (en) * | 1993-08-30 | 2007-03-13 | Vikas V. Padhye | Nucleic acid purification compositions and methods |
| US5491063A (en) * | 1994-09-01 | 1996-02-13 | Hoffmann-La Roche Inc. | Methods for in-solution quenching of fluorescently labeled oligonucleotide probes |
| US6106777A (en) * | 1994-11-09 | 2000-08-22 | Hitachi, Ltd. | DNA analyzing method and device therefor |
| US5858397A (en) * | 1995-10-11 | 1999-01-12 | University Of British Columbia | Liposomal formulations of mitoxantrone |
| EP2369007B1 (en) * | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| WO1998035012A2 (en) * | 1997-02-12 | 1998-08-13 | Chan Eugene Y | Methods and products for analyzing polymers |
| US6403311B1 (en) * | 1997-02-12 | 2002-06-11 | Us Genomics | Methods of analyzing polymers using ordered label strategies |
| US6646149B1 (en) * | 1997-07-15 | 2003-11-11 | Nicolaas M. J. Vermeulin | Polyamine analogues as therapeutic and diagnostic agents |
| GB9725197D0 (en) * | 1997-11-29 | 1998-01-28 | Secr Defence | Detection system |
| KR100386606B1 (ko) * | 2001-02-03 | 2003-06-02 | 엘지전자 주식회사 | Ｄｎａ 검출 방법 및 그 장치 |
| GB0112868D0 (en) * | 2001-05-25 | 2001-07-18 | Secr Defence | Detection system |
| KR100459394B1 (ko) * | 2001-10-30 | 2004-12-03 | 엘지전자 주식회사 | 인터컬레이터를 이용한 핵산의 전기화학발광 검출방법 |
| DE60235491D1 (de) * | 2001-11-28 | 2010-04-08 | Bio Rad Laboratories | Paralleles scoring von polymorphismen mittels amplifikation und fehlerkorrektur |
| DE60229890D1 (de) * | 2002-09-30 | 2008-12-24 | Hoffmann La Roche | Oligonukleotide zur genotypisierung des thymidylat-synthase gens |
| US7354706B2 (en) * | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| WO2005047521A2 (en) * | 2003-11-10 | 2005-05-26 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
-
2002
- 2002-10-10 GB GBGB0223563.8A patent/GB0223563D0/en not_active Ceased
-
2003
- 2003-10-10 AT AT03758305T patent/ATE429513T1/de not_active IP Right Cessation
- 2003-10-10 AU AU2003274316A patent/AU2003274316A1/en not_active Abandoned
- 2003-10-10 CA CA002501636A patent/CA2501636A1/en not_active Abandoned
- 2003-10-10 EP EP03758305A patent/EP1554404B1/en not_active Expired - Lifetime
- 2003-10-10 US US10/530,980 patent/US20060127906A1/en not_active Abandoned
- 2003-10-10 JP JP2004542659A patent/JP2006501836A/ja active Pending
- 2003-10-10 CN CNA2003801056004A patent/CN1723288A/zh active Pending
- 2003-10-10 DE DE60327345T patent/DE60327345D1/de not_active Expired - Lifetime
- 2003-10-10 WO PCT/GB2003/004412 patent/WO2004033726A1/en not_active Ceased
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