JP2005519607A - 改変された遺伝子サイレンシングｒｎａおよびその使用 - Google Patents
改変された遺伝子サイレンシングｒｎａおよびその使用 Download PDFInfo
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| US7429692B2 (en) * | 2004-10-14 | 2008-09-30 | Ceres, Inc. | Sucrose synthase 3 promoter from rice and uses thereof |
| EP1807522B1 (en) | 2004-10-21 | 2013-11-20 | Venganza Inc. | Methods and materials for conferring resistance to pests and pathogens of plants |
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| US8124839B2 (en) * | 2005-06-08 | 2012-02-28 | Ceres, Inc. | Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations |
| PT2431473T (pt) | 2005-09-16 | 2017-02-15 | Monsanto Technology Llc | Métodos para o controlo genético de infestações por insetos em plantas e suas composições |
| WO2007041536A2 (en) * | 2005-09-30 | 2007-04-12 | Ceres, Inc. | Modulating plant tocopherol levels |
| US20090178160A1 (en) * | 2005-10-25 | 2009-07-09 | Joon-Hyun Park | Modulation of Triterpenoid Content in Plants |
| US20070107084A1 (en) | 2005-11-10 | 2007-05-10 | Pioneer Hi-Bred International, Inc. | Dof (DNA binding with one finger) sequences and methods of use |
| US8222482B2 (en) * | 2006-01-26 | 2012-07-17 | Ceres, Inc. | Modulating plant oil levels |
| AU2007212168A1 (en) | 2006-02-09 | 2007-08-16 | Pioneer Hi-Bred International, Inc. | Genes for enhancing nitrogen utilization efficiency in crop plants |
| US20070199090A1 (en) * | 2006-02-22 | 2007-08-23 | Nestor Apuya | Modulating alkaloid biosynthesis |
| WO2007120989A2 (en) * | 2006-02-24 | 2007-10-25 | Ceres, Inc. | Shade regulatory regions |
| US20090222957A1 (en) * | 2006-04-07 | 2009-09-03 | Ceres Inc. | Regulatory protein-regulatory region associations related to alkaloid biosynthesis |
| CA2649734A1 (en) | 2006-04-19 | 2007-11-01 | Pioneer Hi-Bred International, Inc. | Isolated polynucleotide molecules corresponding to mutant and wild-type alleles of the maize d9 gene and methods of use |
| US20100024070A1 (en) * | 2006-05-15 | 2010-01-28 | Steven Craig Bobzin | Modulation of oil levels in plants |
| US20100005549A1 (en) * | 2006-06-14 | 2010-01-07 | Shing Kwok | Increasing uv-b tolerance in plants |
| US20130191941A1 (en) | 2006-07-05 | 2013-07-25 | Shing Kwok | Modulating light response pathways in plants, increasing light-related tolerances in plants, and increasing biomass in plants |
| US8362322B2 (en) | 2006-10-27 | 2013-01-29 | Ceres, Inc. | Modulating lignin in plants |
| US8222388B2 (en) | 2006-11-22 | 2012-07-17 | Ceres, Inc. | Broadly expressing regulatory regions |
| US20100151109A1 (en) * | 2006-12-15 | 2010-06-17 | Amr Saad Ragab | Modulation of plant protein levels |
| WO2008083969A2 (en) | 2007-01-11 | 2008-07-17 | Bayer Bioscience N.V. | Differential expression of subgenome specific alleles in cotton and uses thereof |
| BRPI0807428A2 (pt) | 2007-02-06 | 2014-07-22 | Basf Plant Science Gmbh | Molécula de dsrna, coleção de moléculas dsrna, planta transgênica, métodos para preparar uma planta transgênica, e para conceder resistência a nematódeo a uma planta, e, vetor de expressão |
| EP2152733A2 (en) | 2007-05-25 | 2010-02-17 | CropDesign N.V. | Yield enhancement in plants by modulation of maize alfins |
| ES2651911T3 (es) | 2007-08-14 | 2018-01-30 | Commonwealth Scientific And Industrial Research Organisation | Métodos mejorados de silenciamiento génico |
| US8097712B2 (en) | 2007-11-07 | 2012-01-17 | Beelogics Inc. | Compositions for conferring tolerance to viral disease in social insects, and the use thereof |
| AR069392A1 (es) | 2007-11-20 | 2010-01-20 | Pioneer Hi Bred Int | Genes de senalizacion de etileno en maiz y su modulacion para aumentar la tolerancia al estres en plantas |
| EA020628B1 (ru) | 2008-03-04 | 2014-12-30 | Вашингтон Стейт Юниверсити | Композиции и способы для дифференциальной регуляции ненасыщенности жирных кислот в мембранных липидах и масле семян |
| WO2009143995A1 (en) | 2008-05-26 | 2009-12-03 | Bayer Bioscience N.V. | Methods and means to modify fiber strength in fiber-producing plants |
| ES2603530T3 (es) | 2008-07-21 | 2017-02-28 | Commonwealth Scientific And Industrial Research Organisation | Aceite de semilla de algodón mejorado y usos |
| US8419145B2 (en) * | 2008-07-25 | 2013-04-16 | Eastman Kodak Company | Inkjet printhead and method of printing with multiple drop volumes |
| US8298794B2 (en) * | 2008-10-09 | 2012-10-30 | Ceres, Inc. | Cinnamyl-alcohol dehydrogenases |
| BRPI0920107A2 (pt) | 2008-10-30 | 2015-08-18 | Pioneer Hi Bred Int | Polinucleotídeo isolado, cassete de expressão recombinante, célula hospedeira, planta tansgênica, semente transgênica, método para modular gs em uma planta, método para reduzir atividade de polipeptídeo da enzima gs em uma célula de planta. |
| US8431775B2 (en) | 2008-12-04 | 2013-04-30 | Pioneer Hi Bred International Inc | Methods and compositions for enhanced yield by targeted expression of knotted1 |
| US8309530B2 (en) | 2009-02-04 | 2012-11-13 | Washington State University | Compositions and methods for modulating ghrelin-mediated conditions |
| WO2010101818A1 (en) | 2009-03-02 | 2010-09-10 | Pioneer Hi-Bred International, Inc. | Nac transcriptional activators involved in abiotic stress tolerance |
| BRPI1006273A2 (pt) | 2009-03-20 | 2019-09-24 | Basf Plant Science Gmbh | " vetor de expressão isolado, vegetal transgênico, método para produção de um vegetal transgênico e método para conferir resistência contra nematoide a um vegetal " |
| US20100257634A1 (en) * | 2009-04-03 | 2010-10-07 | Venganza Inc. | Bioassay for gene silencing constructs |
| UA108071C2 (uk) | 2009-04-14 | 2015-03-25 | Піонер Хай-Бред Інтернешнл, Інк. | Спосіб поліпшення витривалості до нестачі азоту у рослини |
| MX2011011678A (es) | 2009-05-04 | 2011-12-06 | Pioneer Hi Bred Int | Mejora de la produccion en plantas mediante la modulacion del factor de transcripcion ap2. |
| CA2760883A1 (en) | 2009-05-05 | 2010-11-11 | Beeologics Inc. | Prevention and treatment of nosema disease in bees |
| CA2735978A1 (en) * | 2009-06-02 | 2010-12-09 | Incorporated Administrative Agency National Agriculture And Food Researc H Organization | Method for simultaneous detection of viroids, pstvd and tcdvd |
| US8933297B2 (en) | 2009-06-15 | 2015-01-13 | Icon Genetics Gmbh | Nicotiana benthamiana plants deficient in xylosyltransferase activity |
| CN102573451A (zh) | 2009-07-20 | 2012-07-11 | 希尔雷斯股份有限公司 | 具有增加的生物质的转基因植物 |
| MX2012001080A (es) | 2009-07-24 | 2012-03-26 | Pionner Hi Bred International Inc | Uso de agrupaciones de componentes del dominio de dimerizacion para modular la arquitectura de las plantas. |
| MX2012003972A (es) | 2009-10-02 | 2012-05-08 | Pionner Hi Bred International Inc | Regulacion a la baja de acc sintasa para mejorar el rendimiento de las plantas. |
| US8962584B2 (en) | 2009-10-14 | 2015-02-24 | Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. | Compositions for controlling Varroa mites in bees |
| BR112012013713B1 (pt) | 2009-12-09 | 2023-12-12 | Basf Plant Science Company Gmbh | Método para produzir uma planta e/ou uma parte dessa resistente a um fungo, construção de vetor e uso da construção de vetor |
| WO2011085062A1 (en) | 2010-01-06 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Identification of diurnal rhythms in photosynthetic and non-photosynthetic tissues from zea mays and use in improving crop plants |
| CA2788036A1 (en) | 2010-02-23 | 2011-09-01 | Basf Plant Science Company Gmbh | Nematode-resistant transgenic plants |
| US9121022B2 (en) | 2010-03-08 | 2015-09-01 | Monsanto Technology Llc | Method for controlling herbicide-resistant plants |
| US9611460B2 (en) | 2010-04-15 | 2017-04-04 | National Research Council Of Canada | Genes and proteins for aromatic polyketide synthesis |
| WO2011140329A1 (en) | 2010-05-06 | 2011-11-10 | Ceres, Inc. | Transgenic plants having increased biomass |
| WO2011139431A1 (en) | 2010-05-06 | 2011-11-10 | Pioneer Hi-Bred International, Inc. | Maize acc synthase 3 gene and protein and uses thereof |
| AU2011276127B2 (en) | 2010-07-08 | 2015-03-19 | University Of Copenhagen | Glucosinolate transporter protein and uses thereof |
| WO2012007945A2 (en) | 2010-07-12 | 2012-01-19 | The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization, (A.R.O.), Volcani Center | Isolated polynucleotides and methods and plants using same for regulating plant acidity |
| US10004797B2 (en) | 2010-10-27 | 2018-06-26 | Harrisvaccines, Inc. | Method of rapidly producing improved vaccines for animals |
| EP2633049B1 (en) | 2010-10-27 | 2018-10-10 | Harrisvaccines Inc | Methods and compositions to protect aquatic invertebrates from disease |
| BR112013010278B1 (pt) | 2010-10-27 | 2020-12-29 | Ceres, Inc | método para produzir uma planta, método para modular a composição de biomassa em uma planta, ácido nucleico isolado e método para alterar a composição de biomassa em uma planta |
| US8822427B2 (en) | 2010-10-27 | 2014-09-02 | Harrisvaccines | Methods and compositions to protect aquatic invertebrates from disease |
| DK2655629T3 (da) | 2010-12-24 | 2024-07-15 | BASF Agricultural Solutions Seed US LLC | Brassica-plante, som omfatter en mutant alcatraz-allel |
| WO2012101118A1 (en) | 2011-01-24 | 2012-08-02 | Bayer Cropscience N.V. | Use of the rd29 promoter or fragments thereof for stress-inducible expression of transgenes in cotton |
| BR112013024337A2 (pt) | 2011-03-23 | 2017-09-26 | Du Pont | locus de traço transgênico complexo em uma planta, planta ou semente, método para produzir em uma planta um locus de traço transgênico complexo e construto de expressão |
| EP2697377A1 (en) | 2011-04-15 | 2014-02-19 | Pioneer Hi-Bred International Inc. | Self-reproducing hybrid plants |
| CA2833876A1 (en) | 2011-04-29 | 2012-11-01 | Pioneer Hi-Bred International, Inc. | Down-regulation of a homeodomain-leucine zipper i-class homeobox gene for improved plant performance |
| WO2012169969A1 (en) | 2011-06-10 | 2012-12-13 | Temasek Life Sciences Laboratory Limited | Genetic manipulation and expression systems for pucciniomycotina and us tilaginom ycotina subphyla |
| EP2721160A2 (en) | 2011-06-14 | 2014-04-23 | Synthon Biopharmaceuticals B.V. | Compositions and methods for making and biocontaining auxotrophic transgenic plants |
| BR112013033176A2 (pt) | 2011-06-21 | 2018-06-12 | E I Du Pont De Nemouras And Company | método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão |
| US9181532B2 (en) | 2011-07-29 | 2015-11-10 | Icon Genetics Gmbh | Production of galactosylated N-glycans in plants |
| US20140215655A1 (en) | 2011-08-12 | 2014-07-31 | Bayer Cropscience Nv | Guard cell-specific expression of transgenes in cotton |
| WO2013040116A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control |
| CN103957697B (zh) | 2011-09-13 | 2017-10-24 | 孟山都技术公司 | 用于杂草控制的方法和组合物 |
| US9840715B1 (en) | 2011-09-13 | 2017-12-12 | Monsanto Technology Llc | Methods and compositions for delaying senescence and improving disease tolerance and yield in plants |
| UA116090C2 (uk) | 2011-09-13 | 2018-02-12 | Монсанто Текнолоджи Ллс | Спосіб та композиція для боротьби з бур'янами (варіанти) |
| CA2848689A1 (en) | 2011-09-13 | 2013-03-21 | Monsanto Technology Llc | Methods and compositions for weed control targeting pds |
| US10760086B2 (en) | 2011-09-13 | 2020-09-01 | Monsanto Technology Llc | Methods and compositions for weed control |
| US10829828B2 (en) | 2011-09-13 | 2020-11-10 | Monsanto Technology Llc | Methods and compositions for weed control |
| US10806146B2 (en) | 2011-09-13 | 2020-10-20 | Monsanto Technology Llc | Methods and compositions for weed control |
| US9920326B1 (en) | 2011-09-14 | 2018-03-20 | Monsanto Technology Llc | Methods and compositions for increasing invertase activity in plants |
| CA2850571C (en) | 2011-10-04 | 2021-01-05 | Icon Genetics Gmbh | Nicotiana benthamiana plants deficient in fucosyltransferase activity |
| BR112014008895A2 (pt) | 2011-10-12 | 2019-09-24 | Bayer Cropscience Ag | plantas com atividade diminuída de uma enzima de desfosforilação do amido |
| BR112014008723A2 (pt) | 2011-10-12 | 2019-09-24 | Bayer Cropscience Ag | plantas com diminuição da atividade de uma enzima de desforilação de àmido |
| CA2853775A1 (en) | 2011-10-31 | 2013-05-10 | Pioneer Hi-Bred International, Inc. | Improving plant drought tolerance, nitrogen use efficiency and yield |
| BR122020026837B1 (pt) | 2011-11-02 | 2021-04-13 | Ceres, Inc | Método de aumento do rendimento de planta em solo contendo níveis elevados de al3+, método de aumento da tolerância de uma planta |
| US10323256B2 (en) | 2011-12-09 | 2019-06-18 | Ceres, Inc. | Transgenic plants having altered biomass composition |
| UA116097C2 (uk) | 2011-12-11 | 2018-02-12 | Зе Стейт Оф Ізраел, Міністрі Оф Агрікалче Енд Руерал Девелопмент, Агрікалчерал Рісьоч Організейшн, (А.Р.О.), Волкані Сентре | Спосіб модуляції провідності устячка рослини |
| EA201491670A1 (ru) | 2012-03-13 | 2015-07-30 | Пайонир Хай-Бред Интернэшнл, Инк. | Генетическое снижение мужской репродуктивной функции у растений |
| WO2013138309A1 (en) | 2012-03-13 | 2013-09-19 | Pioneer Hi-Bred International, Inc. | Genetic reduction of male fertility in plants |
| US10100321B2 (en) | 2012-04-19 | 2018-10-16 | Temasek Life Sciences Laboratory Limited | Methods for increasing cotton fiber length |
| US10240162B2 (en) | 2012-05-24 | 2019-03-26 | A.B. Seeds Ltd. | Compositions and methods for silencing gene expression |
| CA2878287A1 (en) | 2012-07-06 | 2014-01-09 | Bayer Cropscience Nv | Soybean rod1 gene sequences and uses thereof |
| CA2878286C (en) | 2012-07-06 | 2023-06-13 | Bayer Cropscience Nv | Brassica rod1 gene sequences and uses thereof |
| US10119147B2 (en) | 2012-07-06 | 2018-11-06 | Washington State University | Brassica plants with modified seed oil composition |
| EP2885411B1 (en) | 2012-08-16 | 2018-12-05 | VIB vzw | Means and methods for altering the lignin pathway in plants |
| MX364070B (es) | 2012-10-18 | 2019-04-10 | Monsanto Technology Llc | Métodos y composiciones para el control de plagas vegetales. |
| BR112015015975A2 (pt) | 2013-01-01 | 2018-11-06 | A. B. Seeds Ltd. | moléculas de dsrna isoladas e métodos de uso das mesmas para silenciamento das moléculas alvo de interesse. |
| US10683505B2 (en) | 2013-01-01 | 2020-06-16 | Monsanto Technology Llc | Methods of introducing dsRNA to plant seeds for modulating gene expression |
| US10000767B2 (en) | 2013-01-28 | 2018-06-19 | Monsanto Technology Llc | Methods and compositions for plant pest control |
| CN104955947A (zh) | 2013-01-29 | 2015-09-30 | 格拉斯哥大学董事会 | 用于增加植物中胁迫耐受性和生物量的方法和工具 |
| US20160002648A1 (en) | 2013-03-11 | 2016-01-07 | Mei Guo | Genes for improving nutrient uptake and abiotic stress tolerance in plants |
| UA121846C2 (uk) | 2013-03-13 | 2020-08-10 | Монсанто Текнолоджи Ллс | Спосіб та гербіцидна композиція для контролю видів рослини роду lolium |
| UA123082C2 (uk) | 2013-03-13 | 2021-02-17 | Монсанто Текнолоджи Ллс | Спосіб та гербіцидна композиція для боротьби з видами рослин роду sorghum |
| US20160017360A1 (en) | 2013-03-13 | 2016-01-21 | Pioneer Hi-Bred International, Inc. | Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield |
| US20160010101A1 (en) | 2013-03-13 | 2016-01-14 | Pioneer Hi-Bred International, Inc. | Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize |
| US20140304857A1 (en) | 2013-03-14 | 2014-10-09 | Pioneer Hi Bred International Inc | Maize stress related transcription factor 18 and uses thereof |
| US20140283211A1 (en) | 2013-03-14 | 2014-09-18 | Monsanto Technology Llc | Methods and Compositions for Plant Pest Control |
| US10568328B2 (en) | 2013-03-15 | 2020-02-25 | Monsanto Technology Llc | Methods and compositions for weed control |
| WO2014143996A2 (en) | 2013-03-15 | 2014-09-18 | Pioneer Hi-Bred International, Inc. | Compositions and methods of use of acc oxidase polynucleotides and polypeptides |
| CA2904817C (en) | 2013-03-21 | 2022-10-18 | Vib Vzw | Means and methods for the reduction of photorespiration in crops |
| AU2014278519B2 (en) | 2013-06-11 | 2020-09-10 | Syngenta Crop Protection Ag | Methods for generating transgenic plants |
| EP3431606A1 (en) | 2013-07-01 | 2019-01-23 | Bayer CropScience NV | Methods and means for modulating flowering time in monocot plants |
| US9850496B2 (en) | 2013-07-19 | 2017-12-26 | Monsanto Technology Llc | Compositions and methods for controlling Leptinotarsa |
| CN105980567B (zh) | 2013-07-19 | 2021-04-16 | 孟山都技术有限公司 | 用于控制叶甲属的组合物和方法 |
| EA036403B1 (ru) | 2013-09-24 | 2020-11-06 | Басф Се | Белок с активностью целлюлоза:ксилоглюкан-эндотрансглюкозилазы (cxe) и его применение |
| CA2928830A1 (en) | 2013-10-29 | 2015-05-07 | Shai J. Lawit | Self-reproducing hybrid plants |
| ES3008698T3 (en) | 2013-11-04 | 2025-03-24 | Greenlight Biosciences Inc | Compositions and methods for controlling arthropod parasite and pest infestations |
| UA119253C2 (uk) | 2013-12-10 | 2019-05-27 | Біолоджикс, Інк. | Спосіб боротьби із вірусом у кліща varroa та у бджіл |
| UA121462C2 (uk) | 2014-01-15 | 2020-06-10 | Монсанто Текнолоджі Елелсі | Спосіб та композиція для боротьби із бур'янами з використанням полінуклеотидів epsps |
| US20170166920A1 (en) | 2014-01-30 | 2017-06-15 | Two Blades Foundation | Plants with enhanced resistance to phytophthora |
| BR102014004881A2 (pt) | 2014-02-28 | 2016-02-02 | Univ Rio De Janeiro | método para promover um aumento da biomassa e produtividade vegetal e da tolerância à seca |
| US11525143B2 (en) | 2014-02-28 | 2022-12-13 | Universidade Federal Do Rio De Janeiro | Method for promoting an increase in plant biomass, productivity, and drought resistance |
| EP3420809A1 (en) | 2014-04-01 | 2019-01-02 | Monsanto Technology LLC | Compositions and methods for controlling insect pests |
| US9101100B1 (en) | 2014-04-30 | 2015-08-11 | Ceres, Inc. | Methods and materials for high throughput testing of transgene combinations |
| WO2015171603A1 (en) | 2014-05-06 | 2015-11-12 | Two Blades Foundation | Methods for producing plants with enhanced resistance to oomycete pathogens |
| EP3158067B1 (en) | 2014-06-23 | 2020-08-12 | Monsanto Technology LLC | Compositions and methods for regulating gene expression via rna interference |
| WO2015200539A1 (en) | 2014-06-25 | 2015-12-30 | Monsanto Technology Llc | Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression |
| EP3161140A4 (en) | 2014-06-27 | 2017-12-20 | National Research Council of Canada | Cannabichromenic acid synthase from cannabis sativa |
| CA2954201A1 (en) | 2014-07-08 | 2016-01-14 | Vib Vzw | Means and methods to increase plant yield |
| AR101167A1 (es) | 2014-07-10 | 2016-11-30 | Benson Hill Biosystems Inc | Composiciones y métodos para incrementar el crecimiento y rendimiento de las plantas |
| US10480000B2 (en) | 2014-07-15 | 2019-11-19 | Ceres, Inc. | Methods of increasing crop yield under abiotic stress |
| US10378012B2 (en) | 2014-07-29 | 2019-08-13 | Monsanto Technology Llc | Compositions and methods for controlling insect pests |
| CN104232796A (zh) * | 2014-09-22 | 2014-12-24 | 北京出入境检验检疫局检验检疫技术中心 | 检测澳大利亚葡萄类病毒的RT-qPCR寡核苷酸及检测方法 |
| WO2016050512A1 (en) | 2014-10-03 | 2016-04-07 | Bayer Cropscience Nv | Methods and means for increasing stress tolerance and biomass in plants |
| EP3209775A4 (en) | 2014-10-22 | 2018-09-12 | Temasek Life Sciences Laboratory Limited | Terpene synthases from ylang ylang (cananga odorata var. fruticosa) |
| WO2016113333A1 (en) | 2015-01-16 | 2016-07-21 | Bayer Cropscience Nv | Leaf-preferential promoters and uses thereof |
| WO2016118762A1 (en) | 2015-01-22 | 2016-07-28 | Monsanto Technology Llc | Compositions and methods for controlling leptinotarsa |
| WO2016128519A1 (en) | 2015-02-12 | 2016-08-18 | Bayer Cropscience Nv | Shoot apex-preferential promoters and uses thereof |
| WO2016196738A1 (en) | 2015-06-02 | 2016-12-08 | Monsanto Technology Llc | Compositions and methods for delivery of a polynucleotide into a plant |
| EP3302030A4 (en) | 2015-06-03 | 2019-04-24 | Monsanto Technology LLC | METHOD AND COMPOSITIONS FOR INTRODUCING NUCLEIC ACIDS IN PLANTS |
| WO2017025282A1 (en) | 2015-08-07 | 2017-02-16 | Bayer Cropscience Nv | Root-preferential and stress inducible promoter and uses thereof |
| WO2017062790A1 (en) | 2015-10-09 | 2017-04-13 | Two Blades Foundation | Cold shock protein receptors and methods of use |
| US20190153456A1 (en) | 2015-10-16 | 2019-05-23 | Bayer Cropscience Nv | Brassica plants with altered properties in seed production |
| EP3365450A2 (en) | 2015-10-23 | 2018-08-29 | Donald Danforth Plant Science Center | Genes encoding c4 transporter proteins for use in increasing crop plant yield |
| CN105463011B (zh) * | 2015-12-22 | 2019-02-01 | 华南农业大学 | 一种植物生长负调控顺式元件se1及其在调控植物株高中的应用 |
| CA3016487A1 (en) | 2016-03-18 | 2017-09-21 | Marc C. Albertsen | Methods and compositions for producing clonal, non-reduced, non-recombined gametes |
| CN109196105B (zh) | 2016-03-24 | 2022-08-05 | 莱顿大学 | 转染植物和减少随机整合事件的方法 |
| US20190127747A1 (en) | 2016-04-11 | 2019-05-02 | Bayer Cropscience Nv | Seed-specific and endosperm-preferential promoters and uses thereof |
| CA3019984A1 (en) | 2016-04-11 | 2017-10-19 | Bayer Cropscience Nv | Seed-specific and endosperm-preferential promoters and uses thereof |
| BR112018071103A2 (pt) | 2016-04-13 | 2019-02-26 | Bayer Cropscience Nv | promotores específicos de semente e preferenciais em embrião e usos dos mesmos |
| WO2017178367A1 (en) | 2016-04-13 | 2017-10-19 | Bayer Cropscience Nv | Seed- and funiculus-preferential promoters and uses thereof |
| AU2018239406A1 (en) | 2017-03-23 | 2019-09-26 | Basf Se | Anther-specific promoter and uses thereof |
| AR111690A1 (es) | 2017-05-24 | 2019-08-07 | Univ Claude Bernard Lyon | Promotor inducible por roya fúngica |
| MX2020002872A (es) | 2017-09-15 | 2020-10-05 | Commw Scient Ind Res Org | Moléculas de arn. |
| US11279944B2 (en) | 2017-10-24 | 2022-03-22 | BASF Agricultural Solutions Seed US LLC | Of herbicide tolerance to 4-hydroxyphenylpyruvate dioxygenase (HPPD) inhibitors by down-regulation of HPPD expression in soybean |
| WO2019083808A1 (en) | 2017-10-24 | 2019-05-02 | Basf Se | IMPROVING HERBICIDE TOLERANCE AGAINST HPPD INHIBITORS BY REGULATION OF PUTATIVE REDUCED 4-HYDROXYPHENYLPYRUVATE REDUCES IN SOYBEANS |
| EP3502259A1 (en) | 2017-12-19 | 2019-06-26 | Universiteit Leiden | A combinational strategy for reducing random integration events when transfecting plants |
| CA3103906A1 (en) | 2018-06-27 | 2020-01-02 | Basf Se | Thermostable rubisco activase and uses thereof |
| US11473086B2 (en) | 2019-06-19 | 2022-10-18 | Ut-Battelle, Llc | Loss of function alleles of PtEPSP-TF and its regulatory targets in rice |
| WO2021004838A2 (en) | 2019-07-05 | 2021-01-14 | BASF Agricultural Solutions Seed US LLC | Rubisco activase with reduced adp inhibition and uses thereof |
| CA3148950A1 (en) | 2019-07-30 | 2021-02-04 | The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (A.R.O.), Volcani Center | Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby |
| US20210381039A1 (en) * | 2020-05-29 | 2021-12-09 | Front Range Biosciences, Inc. | Methods and compositions for pathogen detection in plants |
| EP4168561A4 (en) * | 2020-07-20 | 2024-08-21 | Flagship Pioneering, Inc. | VIROID-DERIVED POLYNUCLEOTIDES FOR PLANT MODIFICATIONS |
| US20250066865A1 (en) * | 2021-02-19 | 2025-02-27 | Purple City Labs, Inc. | Detection of pathogens in plants |
| WO2023012342A1 (en) | 2021-08-06 | 2023-02-09 | Kws Vegetables B.V. | Durable downy mildew resistance in spinach |
| EP4408860A1 (en) | 2021-10-01 | 2024-08-07 | Basf Se | Wheat plants with an increased yield |
| EP4408162A1 (en) | 2021-10-01 | 2024-08-07 | Basf Se | Plants with improved properties |
| WO2024099765A2 (en) | 2022-11-10 | 2024-05-16 | BASF Agricultural Solutions Seed US LLC | Transcription regulating nucleotide sequences and methods of use |
| EP4477069A1 (en) | 2023-06-16 | 2024-12-18 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Unreduced clonal gamete formation and polyploid genome design in the solanaceae |
| NL2035852B1 (en) | 2023-09-21 | 2025-03-28 | Academisch Ziekenhuis Leiden | Enhancement of t cell mediated therapies |
| CN117625682A (zh) * | 2023-12-04 | 2024-03-01 | 西部(重庆)科学城种质创制大科学中心 | 基于CRISPR-RfxCas13d系统提高马铃薯PSTVd抗性的方法及其产品和应用 |
| CN118995804B (zh) * | 2024-10-23 | 2025-02-28 | 青岛农业大学 | 一种突变型质粒及制备方法和应用 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4873191A (en) * | 1981-06-12 | 1989-10-10 | Ohio University | Genetic transformation of zygotes |
| US5190931A (en) * | 1983-10-20 | 1993-03-02 | The Research Foundation Of State University Of New York | Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA |
| DE3486479T2 (de) | 1983-10-20 | 2001-09-20 | The Research Foundation Of State University Of New York, Albany | Regulierung der Genexpression durch Translationshemmung unter Verwendung von mRNA hindernder Komplementär-RNA |
| US6617496B1 (en) | 1985-10-16 | 2003-09-09 | Monsanto Company | Effecting virus resistance in plants through the use of negative strand RNAs |
| NZ219472A (en) | 1986-03-28 | 1990-08-28 | Calgene Inc | Regulation of phenotype in plant cells using dsdna constructs |
| US5037745A (en) * | 1986-04-01 | 1991-08-06 | University Of Medicine And Dentistry Of New Jersey | Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids |
| AU2811889A (en) | 1987-10-20 | 1989-05-23 | Plant Genetic Systems N.V. | A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants |
| GB8810120D0 (en) | 1988-04-28 | 1988-06-02 | Plant Genetic Systems Nv | Transgenic nuclear male sterile plants |
| WO1992013956A1 (en) | 1991-02-08 | 1992-08-20 | Plant Genetic Systems, N.V. | Stamen-specific promoters from rice |
| US5908779A (en) * | 1993-12-01 | 1999-06-01 | University Of Connecticut | Targeted RNA degradation using nuclear antisense RNA |
| JPH10505233A (ja) | 1994-08-30 | 1998-05-26 | コモンウェルス サイエンティフック アンド インダストリアル リサーチ オーガニゼーション | シルコウイルス由来の植物転写調節因子 |
| CA2234222C (en) | 1995-10-06 | 2007-12-04 | Plant Genetic Systems, N.V. | Seed shattering |
| DE19631919C2 (de) | 1996-08-07 | 1998-07-16 | Deutsches Krebsforsch | Anti-Sinn-RNA mit Sekundärstruktur |
| WO2000049035A1 (en) * | 1999-02-19 | 2000-08-24 | The General Hospital Corporation | Gene silencing |
| US6423885B1 (en) | 1999-08-13 | 2002-07-23 | Commonwealth Scientific And Industrial Research Organization (Csiro) | Methods for obtaining modified phenotypes in plant cells |
| JP2004512020A (ja) | 2000-06-23 | 2004-04-22 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | 組換え構築物ならびにこれを遺伝子発現を減少させる際に用いる方法 |
| WO2002000894A2 (en) | 2000-06-30 | 2002-01-03 | Cropdesign N.V. | Gene silencing vector |
| WO2002010365A2 (en) | 2000-08-02 | 2002-02-07 | The Board Of Regents Of The University Of Nebraska | Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts |
| WO2002059294A1 (en) * | 2001-01-26 | 2002-08-01 | Commonwealth Scientific And Industrial Research O Rganisation | Methods and means for producing efficient silencing construct using recombinational cloning |
-
2003
- 2003-03-12 US US10/385,521 patent/US7576262B2/en not_active Expired - Fee Related
- 2003-03-12 EP EP03743758A patent/EP1487980A4/en not_active Withdrawn
- 2003-03-12 CA CA002478904A patent/CA2478904A1/en not_active Abandoned
- 2003-03-12 AU AU2003209814A patent/AU2003209814B2/en not_active Ceased
- 2003-03-12 WO PCT/AU2003/000292 patent/WO2003076619A1/en not_active Ceased
- 2003-03-12 JP JP2003574823A patent/JP2005519607A/ja active Pending
- 2003-03-12 CN CNA038082551A patent/CN1646687A/zh active Pending
- 2003-03-12 BR BR0308424-8A patent/BR0308424A/pt not_active Application Discontinuation
-
2004
- 2004-08-30 IN IN1268KO2004 patent/IN2004KO01268A/en unknown
-
2008
- 2008-07-18 US US12/219,302 patent/US20090165153A1/en not_active Abandoned
Non-Patent Citations (3)
| Title |
|---|
| JPN6009001593, Ｍｏｌ Ｐｌａｎｔ Ｍｉｃｒｏｂｅ Ｉｎｔｅｒａｃｔ．（２００１），Ｖｏｌ．１４，Ｎｏ．１１，ｐ．１３３２−１３３４ * |
| JPN6009001595, Ｎｕｃｌｅｉｃ Ａｃｉｄｓ Ｒｅｓｅａｒｃｈ（２００１），Ｖｏｌ．２９，Ｎｏ．１１，ｐ．２３９５−２４００ * |
| JPN7009000243, Ｐｌａｎｔ Ｊ．（２００１），Ｖｏｌ．２７，Ｎｏ．６，ｐ．５８１−５９０ * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010521973A (ja) * | 2007-03-21 | 2010-07-01 | ブルックヘブン サイエンス アソシエイツ，エルエルシー | 組み合わされたヘアピン−アンチセンス組成物および発現を調節するための方法 |
| US8796442B2 (en) | 2007-03-21 | 2014-08-05 | Brookhaven Science Associates, Llc. | Combined hairpin-antisense compositions and methods for modulating expression |
| US9193972B2 (en) | 2007-03-21 | 2015-11-24 | Brookhaven Science Associates, Llc | Combined hairpin-antisense compositions and methods for modulating expression |
Also Published As
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| CA2478904A1 (en) | 2003-09-18 |
| BR0308424A (pt) | 2005-02-22 |
| US20030180945A1 (en) | 2003-09-25 |
| EP1487980A4 (en) | 2005-08-10 |
| IN2004KO01268A (enExample) | 2006-05-05 |
| CN1646687A (zh) | 2005-07-27 |
| US7576262B2 (en) | 2009-08-18 |
| AU2003209814A1 (en) | 2003-09-22 |
| US20090165153A1 (en) | 2009-06-25 |
| AU2003209814B2 (en) | 2008-12-04 |
| WO2003076619A1 (en) | 2003-09-18 |
| EP1487980A1 (en) | 2004-12-22 |
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