JP2005517455A5 - - Google Patents
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- Publication number
- JP2005517455A5 JP2005517455A5 JP2003569871A JP2003569871A JP2005517455A5 JP 2005517455 A5 JP2005517455 A5 JP 2005517455A5 JP 2003569871 A JP2003569871 A JP 2003569871A JP 2003569871 A JP2003569871 A JP 2003569871A JP 2005517455 A5 JP2005517455 A5 JP 2005517455A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- target nucleic
- real
- sample
- probe
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 claims 15
- 239000000523 sample Substances 0.000 claims 13
- 108020004707 nucleic acids Proteins 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 10
- 150000007523 nucleic acids Chemical class 0.000 claims 10
- 238000003753 real-time PCR Methods 0.000 claims 6
- 108091093088 Amplicon Proteins 0.000 claims 5
- 239000013612 plasmid Substances 0.000 claims 3
- 238000011002 quantification Methods 0.000 claims 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 2
- 102100037850 Interferon gamma Human genes 0.000 claims 2
- 108010074328 Interferon-gamma Proteins 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims 1
- 108090000695 Cytokines Proteins 0.000 claims 1
- 102000004127 Cytokines Human genes 0.000 claims 1
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 102000003814 Interleukin-10 Human genes 0.000 claims 1
- 108090000174 Interleukin-10 Proteins 0.000 claims 1
- ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 claims 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims 1
- 238000003556 assay Methods 0.000 claims 1
- 239000002299 complementary DNA Substances 0.000 claims 1
- 239000007850 fluorescent dye Substances 0.000 claims 1
- 108020004999 messenger RNA Proteins 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0204022.8A GB0204022D0 (en) | 2002-02-20 | 2002-02-20 | Nucleic acid quantification |
| PCT/GB2003/000079 WO2003070980A2 (en) | 2002-02-20 | 2003-01-10 | Nucleic acid quantification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2005517455A JP2005517455A (ja) | 2005-06-16 |
| JP2005517455A5 true JP2005517455A5 (enExample) | 2006-03-02 |
Family
ID=9931449
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003569871A Pending JP2005517455A (ja) | 2002-02-20 | 2003-01-10 | 核酸定量化 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20050158722A1 (enExample) |
| EP (1) | EP1495136B1 (enExample) |
| JP (1) | JP2005517455A (enExample) |
| AT (1) | ATE347620T1 (enExample) |
| AU (1) | AU2003206993A1 (enExample) |
| DE (1) | DE60310217D1 (enExample) |
| GB (1) | GB0204022D0 (enExample) |
| WO (1) | WO2003070980A2 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0426188D0 (en) * | 2004-11-30 | 2004-12-29 | Leuven K U Res & Dev | DNA repeats |
| FR2904833A1 (fr) * | 2006-08-11 | 2008-02-15 | Bioquanta Sarl | Procede de dosage d'acide nuclieque par fluorescence |
| US8346485B2 (en) | 2008-11-25 | 2013-01-01 | Quest Diagnostics Investments Incorporated | Methods and apparatuses for estimating initial target nucleic acid concentration in a sample by modeling background signal and cycle-dependent amplification efficiency of a polymerase chain reaction |
| CN102363814B (zh) * | 2011-11-23 | 2013-08-28 | 山西农业大学 | 一种绝对荧光定量pcr检测方法 |
| JP6651294B2 (ja) * | 2014-03-19 | 2020-02-19 | ハウス食品グループ本社株式会社 | リアルタイムｐｃｒによる食物アレルゲンの検出方法及びキット |
| GB201720088D0 (en) | 2017-12-01 | 2018-01-17 | Univ Oslo Hf | PCR Controls |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5219727A (en) * | 1989-08-21 | 1993-06-15 | Hoffmann-Laroche Inc. | Quantitation of nucleic acids using the polymerase chain reaction |
| US6691041B2 (en) * | 2000-03-31 | 2004-02-10 | Roche Molecular Systems, Inc. | Method for the efficiency-corrected real-time quantification of nucleic acids |
-
2002
- 2002-02-20 GB GBGB0204022.8A patent/GB0204022D0/en not_active Ceased
-
2003
- 2003-01-10 DE DE60310217T patent/DE60310217D1/de not_active Expired - Lifetime
- 2003-01-10 WO PCT/GB2003/000079 patent/WO2003070980A2/en not_active Ceased
- 2003-01-10 AU AU2003206993A patent/AU2003206993A1/en not_active Abandoned
- 2003-01-10 US US10/503,596 patent/US20050158722A1/en not_active Abandoned
- 2003-01-10 JP JP2003569871A patent/JP2005517455A/ja active Pending
- 2003-01-10 AT AT03704733T patent/ATE347620T1/de not_active IP Right Cessation
- 2003-01-10 EP EP03704733A patent/EP1495136B1/en not_active Expired - Lifetime
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