JP2005503540A5 - - Google Patents
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- JP2005503540A5 JP2005503540A5 JP2002589762A JP2002589762A JP2005503540A5 JP 2005503540 A5 JP2005503540 A5 JP 2005503540A5 JP 2002589762 A JP2002589762 A JP 2002589762A JP 2002589762 A JP2002589762 A JP 2002589762A JP 2005503540 A5 JP2005503540 A5 JP 2005503540A5
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- 239000000203 mixture Substances 0.000 claims description 59
- 238000000034 method Methods 0.000 claims description 44
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 32
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 32
- 229920001184 polypeptide Polymers 0.000 claims description 31
- 239000007787 solid Substances 0.000 claims description 30
- 125000000524 functional group Chemical group 0.000 claims description 29
- 150000001413 amino acids Chemical class 0.000 claims description 19
- 125000003636 chemical group Chemical group 0.000 claims description 8
- 238000004949 mass spectrometry Methods 0.000 claims description 6
- 238000003776 cleavage reaction Methods 0.000 claims description 4
- 230000007017 scission Effects 0.000 claims description 4
- 125000001998 leucyl group Chemical group 0.000 claims 12
- 239000011324 bead Substances 0.000 claims 7
- 239000011521 glass Substances 0.000 claims 7
- 230000004048 modification Effects 0.000 claims 6
- 238000012986 modification Methods 0.000 claims 6
- FLCQLSRLQIPNLM-UHFFFAOYSA-N (2,5-dioxopyrrolidin-1-yl) 2-acetylsulfanylacetate Chemical compound CC(=O)SCC(=O)ON1C(=O)CCC1=O FLCQLSRLQIPNLM-UHFFFAOYSA-N 0.000 claims 5
- YZCKVEUIGOORGS-OUBTZVSYSA-N Deuterium Chemical compound [2H] YZCKVEUIGOORGS-OUBTZVSYSA-N 0.000 claims 5
- AVXURJPOCDRRFD-UHFFFAOYSA-N Hydroxylamine Chemical compound ON AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims 5
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 5
- 230000021736 acetylation Effects 0.000 claims 5
- 238000006640 acetylation reaction Methods 0.000 claims 5
- 229910052805 deuterium Inorganic materials 0.000 claims 5
- 230000013595 glycosylation Effects 0.000 claims 5
- 238000006206 glycosylation reaction Methods 0.000 claims 5
- 230000033444 hydroxylation Effects 0.000 claims 5
- 238000005805 hydroxylation reaction Methods 0.000 claims 5
- -1 iodoacetyl group Chemical group 0.000 claims 5
- 150000002632 lipids Chemical class 0.000 claims 5
- 239000002207 metabolite Substances 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 230000026731 phosphorylation Effects 0.000 claims 5
- 238000006366 phosphorylation reaction Methods 0.000 claims 5
- 230000013823 prenylation Effects 0.000 claims 5
- 150000003141 primary amines Chemical group 0.000 claims 5
- 230000019635 sulfation Effects 0.000 claims 5
- 238000005670 sulfation reaction Methods 0.000 claims 5
- 230000034512 ubiquitination Effects 0.000 claims 5
- 238000010798 ubiquitination Methods 0.000 claims 5
- 238000002372 labelling Methods 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 3
- 239000000126 substance Substances 0.000 claims 3
- 125000003396 thiol group Chemical group [H]S* 0.000 claims 3
- KZNICNPSHKQLFF-UHFFFAOYSA-N dihydromaleimide Natural products O=C1CCC(=O)N1 KZNICNPSHKQLFF-UHFFFAOYSA-N 0.000 claims 2
- 230000000694 effects Effects 0.000 claims 2
- 150000004713 phosphodiesters Chemical class 0.000 claims 2
- 210000004896 polypeptide structure Anatomy 0.000 claims 2
- 230000009870 specific binding Effects 0.000 claims 2
- 229960002317 succinimide Drugs 0.000 claims 2
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 claims 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 125000004185 ester group Chemical group 0.000 claims 1
- 230000000155 isotopic effect Effects 0.000 claims 1
- 125000000913 palmityl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 description 7
- ROHFNLRQFUQHCH-SHJFKSRGSA-N 2-amino-2,3,3,4,5,5,5-heptadeuterio-4-(trideuteriomethyl)pentanoic acid Chemical compound [2H]C([2H])([2H])C([2H])(C([2H])([2H])[2H])C([2H])([2H])C([2H])(N)C(O)=O ROHFNLRQFUQHCH-SHJFKSRGSA-N 0.000 description 2
- 239000005022 packaging material Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000004445 quantitative analysis Methods 0.000 description 2
- 239000007790 solid phase Substances 0.000 description 2
- 102100031680 Beta-catenin-interacting protein 1 Human genes 0.000 description 1
- 101000993469 Homo sapiens Beta-catenin-interacting protein 1 Proteins 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010195 expression analysis Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 238000010183 spectrum analysis Methods 0.000 description 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/858,198 US7183116B2 (en) | 2001-05-14 | 2001-05-14 | Methods for isolation and labeling of sample molecules |
| PCT/US2002/015500 WO2002093131A2 (en) | 2001-05-14 | 2002-05-14 | Methods for isolating and labeling sample molecules |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009113092A Division JP2009175157A (ja) | 2001-05-14 | 2009-05-07 | サンプル分子を単離および標識するための方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2005503540A JP2005503540A (ja) | 2005-02-03 |
| JP2005503540A5 true JP2005503540A5 (https=) | 2008-11-06 |
| JP4414654B2 JP4414654B2 (ja) | 2010-02-10 |
Family
ID=25327723
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002589762A Expired - Lifetime JP4414654B2 (ja) | 2001-05-14 | 2002-05-14 | サンプル分子を単離および標識するための方法 |
| JP2009113092A Withdrawn JP2009175157A (ja) | 2001-05-14 | 2009-05-07 | サンプル分子を単離および標識するための方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2009113092A Withdrawn JP2009175157A (ja) | 2001-05-14 | 2009-05-07 | サンプル分子を単離および標識するための方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US7183116B2 (https=) |
| EP (1) | EP1456632B1 (https=) |
| JP (2) | JP4414654B2 (https=) |
| CN (1) | CN1602422B (https=) |
| AT (1) | ATE495436T1 (https=) |
| AU (1) | AU2002303760B2 (https=) |
| CA (1) | CA2447874C (https=) |
| DE (1) | DE60238940D1 (https=) |
| WO (1) | WO2002093131A2 (https=) |
Families Citing this family (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| EP0963435B1 (en) | 1997-01-08 | 2008-05-28 | Invitrogen Corporation | Methods for production of proteins |
| EP1311858B1 (en) * | 2000-05-05 | 2006-11-02 | Purdue Research Foundation | Affinity selected signature peptides for protein identification and quantification |
| US20030017507A1 (en) * | 2001-05-30 | 2003-01-23 | Johnson Richard S. | Relative protein quantitation phosphoproteins using stable isotope labeling |
| WO2003092581A2 (en) | 2001-07-16 | 2003-11-13 | Hk Pharmaceuticals, Inc. | Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions |
| WO2003027682A2 (en) * | 2001-09-27 | 2003-04-03 | Purdue Research Foundation | Controlling isotope effects during fractionation of analytes |
| US8614303B2 (en) * | 2001-12-11 | 2013-12-24 | University Of Medicine And Dentistry | Diagnostic methods for ubiquinated protein profiling |
| CN1617938A (zh) | 2002-01-16 | 2005-05-18 | 戴诺生物技术有限公司 | 从单个样品中分离核酸和蛋白质的方法 |
| AU2003220188A1 (en) * | 2002-03-11 | 2003-09-29 | President And Fellows Of Harvard College | Detection and quantification of modified proteins |
| AU2003224674A1 (en) * | 2002-03-11 | 2003-09-29 | Hk Pharmaceuticals, Inc. | Compounds and methods for analyzing the proteome |
| EP1514107B1 (en) * | 2002-06-03 | 2013-05-15 | The Institute for Systems Biology | Methods for quantitative proteome analysis of glycoproteins |
| US20070269895A1 (en) * | 2002-06-03 | 2007-11-22 | The Institute For Systems Biology | Methods for quantitative proteome analysis of glycoproteins |
| JP2004170195A (ja) * | 2002-11-19 | 2004-06-17 | Reverse Proteomics Research Institute Co Ltd | タンパク質の固定化方法 |
| GB0229287D0 (en) * | 2002-12-16 | 2003-01-22 | Dna Res Innovations Ltd | Polyfunctional reagents |
| WO2004064972A2 (en) * | 2003-01-16 | 2004-08-05 | Hk Pharmaceuticals, Inc. | Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions |
| WO2005007677A2 (en) * | 2003-07-10 | 2005-01-27 | The Institute For Systems Biology | Affinity capture of peptides by microarray and related methods |
| US20050113584A1 (en) * | 2003-07-14 | 2005-05-26 | Irm Llc | Methods for the preparation of rhodamine |
| WO2005007663A2 (en) * | 2003-07-14 | 2005-01-27 | Irm Llc | Fluorogenic enzyme substrates and uses thereof |
| WO2005015239A2 (en) * | 2003-08-07 | 2005-02-17 | Cornell Research Foundation, Inc. | Method for n-terminal labeling of proteins |
| JP4363935B2 (ja) * | 2003-09-17 | 2009-11-11 | 株式会社豊田中央研究所 | 生体物質の光固定化・回収方法及び表面プラズモン共鳴センサー |
| EP1668146B1 (en) | 2003-09-25 | 2014-11-12 | Life Technologies Corporation | Homogeneous populations of molecules |
| WO2006017208A1 (en) * | 2004-07-12 | 2006-02-16 | Applera Corporation | Mass tags for quantitative analyses |
| US20070048752A1 (en) * | 2004-07-12 | 2007-03-01 | Applera Corporation | Mass tags for quantitative analyses |
| WO2006011673A1 (ja) * | 2004-07-30 | 2006-02-02 | Reverse Proteomics Research Institute Co., Ltd. | 光切断型リンカーを利用したリガンド固定化固相担体 |
| JP4635532B2 (ja) * | 2004-09-14 | 2011-02-23 | 株式会社ニコン | 生体分子の分析および回収方法 |
| US20060134694A1 (en) * | 2004-12-22 | 2006-06-22 | Intel Corporation | Methods of protein profiling by thiolation |
| WO2006094185A2 (en) * | 2005-03-03 | 2006-09-08 | Dmi Biosciences Inc. | Quantification of proteins |
| WO2007000669A2 (en) * | 2005-06-07 | 2007-01-04 | Centre National De La Recherche Scientifique (Cnrs) | Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections |
| GB0515323D0 (en) * | 2005-07-26 | 2005-08-31 | Electrophoretics Ltd | Mass labels |
| US7906341B2 (en) * | 2006-06-30 | 2011-03-15 | Dh Technologies Development Pte, Ltd. | Methods, mixtures, kits and compositions pertaining to analyte determination |
| EP2069386A4 (en) | 2006-07-21 | 2009-10-28 | Life Technologies Corp | PROTEIN MOLECULAR WEIGHT STANDARDS WITH HIGH DISINFECTION |
| DK2108111T3 (da) * | 2006-12-07 | 2019-07-15 | Definitek Inc | Fastfase- og katalyseaktiveret automatiseret isotopfortynding og specieret isotopfortyndingsmassespektrometri |
| US7728287B2 (en) * | 2007-03-01 | 2010-06-01 | Lawrence Livermore National Security, Llc | Imaging mass spectrometer with mass tags |
| EP2456910B1 (en) * | 2009-07-22 | 2016-05-04 | Agency For Science, Technology And Research | Differentiation of isobaric amino acids and other species |
| WO2012027717A2 (en) | 2010-08-27 | 2012-03-01 | The Texas A&M University System | Flourescence labeling reagents and uses thereof |
| US8492163B2 (en) | 2011-01-31 | 2013-07-23 | Dh Technologies Development Pte. Ltd. | Methods, mixtures, kits and compositions pertaining to analyte determination |
| GB2528643A (en) * | 2014-07-08 | 2016-02-03 | Adc Biotechnology Ltd | Method of synthesising ADCs |
| JP2017020896A (ja) * | 2015-07-10 | 2017-01-26 | 国立大学法人北陸先端科学技術大学院大学 | Ｎ末端標識剤、これを用いた蛍光標識タンパク質及びその製造方法 |
| US20180217156A1 (en) * | 2015-07-29 | 2018-08-02 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Means and methods for a sample preparation, especially for mass spectrometry |
| CN110687297B (zh) * | 2018-07-06 | 2023-08-22 | 复旦大学 | 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法 |
| GB201913598D0 (en) * | 2019-09-20 | 2019-11-06 | Univ Birmingham | Labelling of biomolecules |
| CN113075114B (zh) * | 2019-12-17 | 2022-07-01 | 北京大学 | 一种用于单细胞分析的有机质谱流式分析方法 |
| US20230384314A1 (en) * | 2020-10-16 | 2023-11-30 | Lmx Medtech Llc | Oxidative Mass Labeling |
| CN119569971B (zh) * | 2025-02-07 | 2025-05-27 | 吉林大学 | 一种用于蛋白质磷酸化和棕榈酰化修饰同时筛选的Janus材料的制备方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5986076A (en) | 1994-05-11 | 1999-11-16 | Trustees Of Boston University | Photocleavable agents and conjugates for the detection and isolation of biomolecules |
| EP0776330B1 (en) | 1994-06-23 | 2003-08-20 | Affymax Technologies N.V. | Photolabile compounds and methods for their use |
| EP1002128A1 (en) | 1997-07-11 | 2000-05-24 | Brax Genomics Limited | Characterising nucleic acids |
| US6670194B1 (en) | 1998-08-25 | 2003-12-30 | University Of Washington | Rapid quantitative analysis of proteins or protein function in complex mixtures |
| AU6689401A (en) | 2000-06-12 | 2001-12-24 | Univ Washington | Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis |
| AU4338502A (en) | 2000-10-23 | 2002-06-24 | Genetics Inst | Acid-labile isotope-coded extractant (alice) and its use in quantitative mass spectrometric analysis of protein mixtures |
-
2001
- 2001-05-14 US US09/858,198 patent/US7183116B2/en not_active Expired - Lifetime
-
2002
- 2002-05-14 CA CA2447874A patent/CA2447874C/en not_active Expired - Lifetime
- 2002-05-14 US US10/477,619 patent/US7364911B2/en not_active Expired - Lifetime
- 2002-05-14 AU AU2002303760A patent/AU2002303760B2/en not_active Expired
- 2002-05-14 DE DE60238940T patent/DE60238940D1/de not_active Expired - Lifetime
- 2002-05-14 JP JP2002589762A patent/JP4414654B2/ja not_active Expired - Lifetime
- 2002-05-14 CN CN02814077.XA patent/CN1602422B/zh not_active Expired - Lifetime
- 2002-05-14 AT AT02731818T patent/ATE495436T1/de not_active IP Right Cessation
- 2002-05-14 EP EP02731818A patent/EP1456632B1/en not_active Expired - Lifetime
- 2002-05-14 WO PCT/US2002/015500 patent/WO2002093131A2/en not_active Ceased
-
2003
- 2003-07-07 US US10/615,320 patent/US20040110186A1/en not_active Abandoned
-
2009
- 2009-05-07 JP JP2009113092A patent/JP2009175157A/ja not_active Withdrawn
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