CA2447874C - Methods for isolating and labeling sample molecules - Google Patents

Methods for isolating and labeling sample molecules Download PDF

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Publication number
CA2447874C
CA2447874C CA2447874A CA2447874A CA2447874C CA 2447874 C CA2447874 C CA 2447874C CA 2447874 A CA2447874 A CA 2447874A CA 2447874 A CA2447874 A CA 2447874A CA 2447874 C CA2447874 C CA 2447874C
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CA
Canada
Prior art keywords
group
tag
polypeptide
sample molecule
composition
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2447874A
Other languages
English (en)
French (fr)
Other versions
CA2447874A1 (en
Inventor
Rudolf H. Aebersold
Huilin Zhou
Beate Rist
George J. Vella
Subhasish Purkayastha
Sasi Pillai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DH Technologies Development Pte Ltd
University of Washington
Institute for Systems Biology
Original Assignee
DH Technologies Development Pte Ltd
University of Washington
Institute for Systems Biology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DH Technologies Development Pte Ltd, University of Washington, Institute for Systems Biology filed Critical DH Technologies Development Pte Ltd
Publication of CA2447874A1 publication Critical patent/CA2447874A1/en
Application granted granted Critical
Publication of CA2447874C publication Critical patent/CA2447874C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/15—Non-radioactive isotope labels, e.g. for detection by mass spectrometry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/24—Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Peptides Or Proteins (AREA)
CA2447874A 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules Expired - Lifetime CA2447874C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/858,198 US7183116B2 (en) 2001-05-14 2001-05-14 Methods for isolation and labeling of sample molecules
US09/858,198 2001-05-14
PCT/US2002/015500 WO2002093131A2 (en) 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules

Publications (2)

Publication Number Publication Date
CA2447874A1 CA2447874A1 (en) 2002-11-21
CA2447874C true CA2447874C (en) 2010-08-31

Family

ID=25327723

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2447874A Expired - Lifetime CA2447874C (en) 2001-05-14 2002-05-14 Methods for isolating and labeling sample molecules

Country Status (9)

Country Link
US (3) US7183116B2 (https=)
EP (1) EP1456632B1 (https=)
JP (2) JP4414654B2 (https=)
CN (1) CN1602422B (https=)
AT (1) ATE495436T1 (https=)
AU (1) AU2002303760B2 (https=)
CA (1) CA2447874C (https=)
DE (1) DE60238940D1 (https=)
WO (1) WO2002093131A2 (https=)

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EP0963435B1 (en) 1997-01-08 2008-05-28 Invitrogen Corporation Methods for production of proteins
EP1311858B1 (en) * 2000-05-05 2006-11-02 Purdue Research Foundation Affinity selected signature peptides for protein identification and quantification
US20030017507A1 (en) * 2001-05-30 2003-01-23 Johnson Richard S. Relative protein quantitation phosphoproteins using stable isotope labeling
WO2003092581A2 (en) 2001-07-16 2003-11-13 Hk Pharmaceuticals, Inc. Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2003027682A2 (en) * 2001-09-27 2003-04-03 Purdue Research Foundation Controlling isotope effects during fractionation of analytes
US8614303B2 (en) * 2001-12-11 2013-12-24 University Of Medicine And Dentistry Diagnostic methods for ubiquinated protein profiling
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
AU2003220188A1 (en) * 2002-03-11 2003-09-29 President And Fellows Of Harvard College Detection and quantification of modified proteins
AU2003224674A1 (en) * 2002-03-11 2003-09-29 Hk Pharmaceuticals, Inc. Compounds and methods for analyzing the proteome
EP1514107B1 (en) * 2002-06-03 2013-05-15 The Institute for Systems Biology Methods for quantitative proteome analysis of glycoproteins
US20070269895A1 (en) * 2002-06-03 2007-11-22 The Institute For Systems Biology Methods for quantitative proteome analysis of glycoproteins
JP2004170195A (ja) * 2002-11-19 2004-06-17 Reverse Proteomics Research Institute Co Ltd タンパク質の固定化方法
GB0229287D0 (en) * 2002-12-16 2003-01-22 Dna Res Innovations Ltd Polyfunctional reagents
WO2004064972A2 (en) * 2003-01-16 2004-08-05 Hk Pharmaceuticals, Inc. Capture compounds, collections thereof and methods for analyzing the proteome and complex compositions
WO2005007677A2 (en) * 2003-07-10 2005-01-27 The Institute For Systems Biology Affinity capture of peptides by microarray and related methods
US20050113584A1 (en) * 2003-07-14 2005-05-26 Irm Llc Methods for the preparation of rhodamine
WO2005007663A2 (en) * 2003-07-14 2005-01-27 Irm Llc Fluorogenic enzyme substrates and uses thereof
WO2005015239A2 (en) * 2003-08-07 2005-02-17 Cornell Research Foundation, Inc. Method for n-terminal labeling of proteins
JP4363935B2 (ja) * 2003-09-17 2009-11-11 株式会社豊田中央研究所 生体物質の光固定化・回収方法及び表面プラズモン共鳴センサー
EP1668146B1 (en) 2003-09-25 2014-11-12 Life Technologies Corporation Homogeneous populations of molecules
WO2006017208A1 (en) * 2004-07-12 2006-02-16 Applera Corporation Mass tags for quantitative analyses
US20070048752A1 (en) * 2004-07-12 2007-03-01 Applera Corporation Mass tags for quantitative analyses
WO2006011673A1 (ja) * 2004-07-30 2006-02-02 Reverse Proteomics Research Institute Co., Ltd. 光切断型リンカーを利用したリガンド固定化固相担体
JP4635532B2 (ja) * 2004-09-14 2011-02-23 株式会社ニコン 生体分子の分析および回収方法
US20060134694A1 (en) * 2004-12-22 2006-06-22 Intel Corporation Methods of protein profiling by thiolation
WO2006094185A2 (en) * 2005-03-03 2006-09-08 Dmi Biosciences Inc. Quantification of proteins
WO2007000669A2 (en) * 2005-06-07 2007-01-04 Centre National De La Recherche Scientifique (Cnrs) Use of conjugates with linkers cleavable by photodissociation or fragmentation for mass spectrometry analysis of tissue sections
GB0515323D0 (en) * 2005-07-26 2005-08-31 Electrophoretics Ltd Mass labels
US7906341B2 (en) * 2006-06-30 2011-03-15 Dh Technologies Development Pte, Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
EP2069386A4 (en) 2006-07-21 2009-10-28 Life Technologies Corp PROTEIN MOLECULAR WEIGHT STANDARDS WITH HIGH DISINFECTION
DK2108111T3 (da) * 2006-12-07 2019-07-15 Definitek Inc Fastfase- og katalyseaktiveret automatiseret isotopfortynding og specieret isotopfortyndingsmassespektrometri
US7728287B2 (en) * 2007-03-01 2010-06-01 Lawrence Livermore National Security, Llc Imaging mass spectrometer with mass tags
EP2456910B1 (en) * 2009-07-22 2016-05-04 Agency For Science, Technology And Research Differentiation of isobaric amino acids and other species
WO2012027717A2 (en) 2010-08-27 2012-03-01 The Texas A&M University System Flourescence labeling reagents and uses thereof
US8492163B2 (en) 2011-01-31 2013-07-23 Dh Technologies Development Pte. Ltd. Methods, mixtures, kits and compositions pertaining to analyte determination
GB2528643A (en) * 2014-07-08 2016-02-03 Adc Biotechnology Ltd Method of synthesising ADCs
JP2017020896A (ja) * 2015-07-10 2017-01-26 国立大学法人北陸先端科学技術大学院大学 Ｎ末端標識剤、これを用いた蛍光標識タンパク質及びその製造方法
US20180217156A1 (en) * 2015-07-29 2018-08-02 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Means and methods for a sample preparation, especially for mass spectrometry
CN110687297B (zh) * 2018-07-06 2023-08-22 复旦大学 基于稳定同位素半胱氨酸代谢标记的棕榈酰化修饰蛋白质定量分析方法
GB201913598D0 (en) * 2019-09-20 2019-11-06 Univ Birmingham Labelling of biomolecules
CN113075114B (zh) * 2019-12-17 2022-07-01 北京大学 一种用于单细胞分析的有机质谱流式分析方法
US20230384314A1 (en) * 2020-10-16 2023-11-30 Lmx Medtech Llc Oxidative Mass Labeling
CN119569971B (zh) * 2025-02-07 2025-05-27 吉林大学 一种用于蛋白质磷酸化和棕榈酰化修饰同时筛选的Janus材料的制备方法

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US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5986076A (en) 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
EP0776330B1 (en) 1994-06-23 2003-08-20 Affymax Technologies N.V. Photolabile compounds and methods for their use
EP1002128A1 (en) 1997-07-11 2000-05-24 Brax Genomics Limited Characterising nucleic acids
US6670194B1 (en) 1998-08-25 2003-12-30 University Of Washington Rapid quantitative analysis of proteins or protein function in complex mixtures
AU6689401A (en) 2000-06-12 2001-12-24 Univ Washington Selective labeling and isolation of phosphopeptides and applications to proteomeanalysis
AU4338502A (en) 2000-10-23 2002-06-24 Genetics Inst Acid-labile isotope-coded extractant (alice) and its use in quantitative mass spectrometric analysis of protein mixtures

Also Published As

Publication number Publication date
US20040110186A1 (en) 2004-06-10
EP1456632A2 (en) 2004-09-15
EP1456632A4 (en) 2005-04-20
AU2002303760B2 (en) 2008-04-10
US20020168644A1 (en) 2002-11-14
CN1602422B (zh) 2011-06-08
US7364911B2 (en) 2008-04-29
CA2447874A1 (en) 2002-11-21
US20040265810A1 (en) 2004-12-30
EP1456632B1 (en) 2011-01-12
WO2002093131A3 (en) 2004-05-27
WO2002093131A2 (en) 2002-11-21
JP2005503540A (ja) 2005-02-03
JP2009175157A (ja) 2009-08-06
ATE495436T1 (de) 2011-01-15
CN1602422A (zh) 2005-03-30
JP4414654B2 (ja) 2010-02-10
DE60238940D1 (de) 2011-02-24
US7183116B2 (en) 2007-02-27

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