JP2004535820A5 - - Google Patents
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- JP2004535820A5 JP2004535820A5 JP2003515654A JP2003515654A JP2004535820A5 JP 2004535820 A5 JP2004535820 A5 JP 2004535820A5 JP 2003515654 A JP2003515654 A JP 2003515654A JP 2003515654 A JP2003515654 A JP 2003515654A JP 2004535820 A5 JP2004535820 A5 JP 2004535820A5
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- cells
- cardiomyocytes
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- cardiomyocyte
- endoderm
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Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPR6560A AUPR656001A0 (en) | 2001-07-24 | 2001-07-24 | Methods of inducing differentiation of stem cells |
| AUPR6560 | 2001-07-24 | ||
| AUPS1180 | 2002-03-18 | ||
| AUPS1180A AUPS118002A0 (en) | 2002-03-18 | 2002-03-18 | Methods of inducing differentiation of stem cells |
| PCT/AU2002/000978 WO2003010303A1 (en) | 2001-07-24 | 2002-07-23 | Methods of inducing differentiation of stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004535820A JP2004535820A (ja) | 2004-12-02 |
| JP2004535820A5 true JP2004535820A5 (enExample) | 2011-06-02 |
| JP5479661B2 JP5479661B2 (ja) | 2014-04-23 |
Family
ID=25646758
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003515654A Expired - Fee Related JP5479661B2 (ja) | 2001-07-24 | 2002-07-23 | 幹細胞の分化を誘導する方法 |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US8728457B2 (enExample) |
| EP (1) | EP1421182A4 (enExample) |
| JP (1) | JP5479661B2 (enExample) |
| AU (1) | AU2002317039B2 (enExample) |
| CA (1) | CA2452256A1 (enExample) |
| GB (2) | GB2394958B (enExample) |
| WO (1) | WO2003010303A1 (enExample) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1379869A4 (en) * | 2001-03-15 | 2004-08-11 | Xiao Yong Fu | METHOD FOR THERAPEUTICALLY TREATING A CLINICALLY DETECTED CARDIOPATHOLOGICAL FORM IN A MAMMAL |
| GB2393734B (en) * | 2001-07-12 | 2005-07-27 | Geron Corp | Cells of the cardiomyocyte lineage produced from human pluripotent stem cells |
| US7732199B2 (en) | 2001-07-12 | 2010-06-08 | Geron Corporation | Process for making transplantable cardiomyocytes from human embryonic stem cells |
| EP1572984B8 (en) | 2002-12-16 | 2016-06-01 | Technion Research & Development Foundation Ltd. | Feeder-free, xeno-free culture system for human embryonic stem cells |
| AU2003901099A0 (en) * | 2003-03-11 | 2003-03-27 | Es Cell International Pte Ltd. | Methods of inducing differentiation of stem cells |
| WO2004113515A2 (en) * | 2003-06-20 | 2004-12-29 | Axiogenesis Ag | Tissue modeling in embryonic stem (es) cell system |
| JP4667241B2 (ja) * | 2003-06-27 | 2011-04-06 | 旭化成株式会社 | 細胞分化抑制剤、これを用いた細胞培養方法、培養液及び培養された細胞株 |
| US7452718B2 (en) * | 2004-03-26 | 2008-11-18 | Geron Corporation | Direct differentiation method for making cardiomyocytes from human embryonic stem cells |
| US20050214938A1 (en) * | 2004-03-26 | 2005-09-29 | Gold Joseph D | Cardiac bodies: clusters of spontaneously contracting cells for regenerating cardiac function |
| EP1740945B1 (en) | 2004-04-07 | 2018-09-19 | Ncardia AG | Non-invasive, in vitro functional tissue assay systems |
| DK1745144T3 (da) | 2004-05-11 | 2011-02-07 | Axiogenesis Ag | Analysefremgangsmåde til opsporing af lægemidler på grundlag af in vitro-differentierede celler |
| CA2569242A1 (en) | 2004-06-01 | 2005-12-15 | Es Cell International Pte Ltd | Improved cardiomyocyte differentiation |
| US20050277124A1 (en) * | 2004-06-10 | 2005-12-15 | White Steven M | Cardiac conduction system cells and uses thereof |
| CN101228264B (zh) | 2005-06-22 | 2017-12-26 | 阿斯特利亚斯生物治疗股份公司 | 使灵长类多能干细胞分化成心肌细胞系细胞 |
| US20070054397A1 (en) * | 2005-08-26 | 2007-03-08 | Harald Ott | Adult cardiac uncommitted progenitor cells |
| US8476070B2 (en) | 2005-08-29 | 2013-07-02 | Technion Research & Development Foundation Limited | Media for culturing stem cells |
| US7732202B2 (en) * | 2005-10-21 | 2010-06-08 | International Stem Cell Corporation | Oxygen tension for the parthenogenic activation of human oocytes for the production of human embryonic stem cells |
| TW200734462A (en) | 2006-03-08 | 2007-09-16 | In Motion Invest Ltd | Regulating stem cells |
| ES2704401T3 (es) | 2006-08-02 | 2019-03-18 | Technion Res & Dev Foundation | Métodos de expansión de células madre embrionarias en un cultivo en suspensión |
| JP5230969B2 (ja) * | 2007-06-14 | 2013-07-10 | 公益財団法人先端医療振興財団 | 心筋芽細胞を含むシート |
| CN105400735A (zh) * | 2008-01-30 | 2016-03-16 | 阿斯特利亚斯生物治疗股份公司 | 用于培养干细胞衍生的心肌细胞的合成表面 |
| RU2417052C2 (ru) * | 2008-08-06 | 2011-04-27 | Владимир Михайлович Музалевский | Способ стимулирования зоны роста стволовых клеток по методу музалевского |
| KR101109125B1 (ko) | 2009-03-24 | 2012-02-15 | 한국과학기술연구원 | 줄기세포를 혈관세포로 분화시키는 방법 및 이를 이용한 생체 내 혈관신생 유도 |
| WO2010110596A2 (en) * | 2009-03-24 | 2010-09-30 | Korea Institute Of Science And Technology | Method for differentiation of stem cells into vascular cells and the induction of angiogenesis using the same |
| US9018010B2 (en) | 2009-11-12 | 2015-04-28 | Technion Research & Development Foundation Limited | Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state |
| EP2521587B1 (en) * | 2010-01-08 | 2020-04-08 | Wake Forest University Health Sciences | Delivery system |
| US9428735B2 (en) | 2010-02-25 | 2016-08-30 | The Johns Hopkins University | Smooth muscle-like cells (SMLCs) dervided from human pluripotent stem cells |
| US8895291B2 (en) | 2010-10-08 | 2014-11-25 | Terumo Bct, Inc. | Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions |
| EP2683328B1 (en) | 2011-03-07 | 2017-11-08 | Wake Forest University Health Sciences | Delivery system |
| WO2013033213A1 (en) | 2011-08-30 | 2013-03-07 | The J. David Gladstone Institutes | Methods for generating cardiomyocytes |
| US9045038B2 (en) | 2011-12-22 | 2015-06-02 | Eaton Corporation | Liquid trap with integral jet pump |
| WO2013130820A1 (en) * | 2012-02-29 | 2013-09-06 | The Johns Hopkins University | Method for guiding the derivation of endothelial cells from human pluripotent stem cells employing two-dimensional, feeder-free differentiation |
| US9506037B2 (en) | 2013-03-15 | 2016-11-29 | The Johns Hopkins University | Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix |
| US9994825B2 (en) | 2013-03-15 | 2018-06-12 | The Johns Hopkins University | Self-organized vascular networks from human pluripotent stem cells in a synthetic matrix |
| JP6612227B2 (ja) | 2013-11-16 | 2019-11-27 | テルモ ビーシーティー、インコーポレーテッド | バイオリアクターにおける細胞増殖 |
| US11008547B2 (en) | 2014-03-25 | 2021-05-18 | Terumo Bct, Inc. | Passive replacement of media |
| WO2016049421A1 (en) | 2014-09-26 | 2016-03-31 | Terumo Bct, Inc. | Scheduled feed |
| WO2017004592A1 (en) | 2015-07-02 | 2017-01-05 | Terumo Bct, Inc. | Cell growth with mechanical stimuli |
| WO2017205667A1 (en) | 2016-05-25 | 2017-11-30 | Terumo Bct, Inc. | Cell expansion |
| US11104874B2 (en) | 2016-06-07 | 2021-08-31 | Terumo Bct, Inc. | Coating a bioreactor |
| US11685883B2 (en) | 2016-06-07 | 2023-06-27 | Terumo Bct, Inc. | Methods and systems for coating a cell growth surface |
| WO2018076060A1 (en) | 2016-10-26 | 2018-05-03 | Genea Ip Holdings Pty Ltd | Improved generation of muscle lineage cells and therapeutic uses thereof |
| US11746331B2 (en) * | 2017-01-27 | 2023-09-05 | Kaneka Corporation | Endodermal cell population, and method for producing cell population of any of three germ layers from pluripotent cell |
| US12234441B2 (en) | 2017-03-31 | 2025-02-25 | Terumo Bct, Inc. | Cell expansion |
| WO2018184028A2 (en) | 2017-03-31 | 2018-10-04 | Terumo Bct, Inc. | Cell expansion |
| US11624046B2 (en) | 2017-03-31 | 2023-04-11 | Terumo Bct, Inc. | Cell expansion |
| US12263275B2 (en) | 2018-06-13 | 2025-04-01 | Miromatrix Medical Inc. | Fistula filler and deployment system |
| EP3696617B1 (fr) * | 2019-02-14 | 2023-07-05 | Glashütter Uhrenbetrieb GmbH | Mecanisme d'affichage de mois et d'annee bissextile pour piece d'horlogerie |
| EP3976069A4 (en) | 2019-05-31 | 2023-07-19 | Figene, LLC | FIBROBLAST THERAPY FOR THE TREATMENT OF DUCHENNE MUSCULAR DYSTROPHY |
| GB2619893A (en) | 2021-03-23 | 2023-12-20 | Terumo Bct Inc | Cell capture and expansion |
| US12209689B2 (en) | 2022-02-28 | 2025-01-28 | Terumo Kabushiki Kaisha | Multiple-tube pinch valve assembly |
| USD1099116S1 (en) | 2022-09-01 | 2025-10-21 | Terumo Bct, Inc. | Display screen or portion thereof with a graphical user interface for displaying cell culture process steps and measurements of an associated bioreactor device |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6331406B1 (en) * | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
| CA2324591A1 (en) * | 1998-04-09 | 1999-10-21 | Bresagen Limited | Cell differentiation/proliferation and maintenance factor and uses thereof |
| US6667176B1 (en) * | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| IL129966A (en) * | 1999-05-14 | 2009-12-24 | Technion Res & Dev Foundation | ISOLATED HUMAN EMBRYOID BODIES (hEB) DERIVED FROM HUMAN EMBRYONIC STEM CELLS |
| US6280718B1 (en) * | 1999-11-08 | 2001-08-28 | Wisconsin Alumni Reasearch Foundation | Hematopoietic differentiation of human pluripotent embryonic stem cells |
| WO2001048151A1 (en) * | 1999-12-28 | 2001-07-05 | Kyowa Hakko Kogyo Co., Ltd. | Cells capable of differentiating into heart muscle cells |
| WO2002014469A2 (en) * | 2000-08-15 | 2002-02-21 | Geron Corporation | Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells |
| GB2393734B (en) * | 2001-07-12 | 2005-07-27 | Geron Corp | Cells of the cardiomyocyte lineage produced from human pluripotent stem cells |
| MXPA04005010A (es) * | 2001-11-26 | 2005-04-08 | Advanced Cell Tech Inc | METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS. |
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2002
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- 2002-07-23 CA CA002452256A patent/CA2452256A1/en not_active Abandoned
- 2002-07-23 GB GB0404097A patent/GB2394958B/en not_active Expired - Fee Related
- 2002-07-23 WO PCT/AU2002/000978 patent/WO2003010303A1/en not_active Ceased
- 2002-07-23 EP EP02744938A patent/EP1421182A4/en not_active Withdrawn
- 2002-07-23 AU AU2002317039A patent/AU2002317039B2/en not_active Ceased
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2004
- 2004-01-14 US US10/758,554 patent/US8728457B2/en not_active Expired - Fee Related
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2005
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2014
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