JP2004535189A - 組換えタンパク質の高レベルの大規模生成のための組成物および方法 - Google Patents

組換えタンパク質の高レベルの大規模生成のための組成物および方法 Download PDF

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JP2004535189A
JP2004535189A JP2003502199A JP2003502199A JP2004535189A JP 2004535189 A JP2004535189 A JP 2004535189A JP 2003502199 A JP2003502199 A JP 2003502199A JP 2003502199 A JP2003502199 A JP 2003502199A JP 2004535189 A JP2004535189 A JP 2004535189A
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polypeptide
vector
host cell
ucoe
composition
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JP2004535189A5 (enExample
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トリッシュ ベントン，
クリストファー ロバート ベビントン，
カーラ アン ヘニング，
デービッド ジェイ． キング，
ロバート クロンビー，
シャオ シャン，
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エムエル ラボラトリーズ ピーエルシー
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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    • C12N2800/00—Nucleic acids vectors
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    • C12N2800/108—Plasmid DNA episomal vectors
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
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    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
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    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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JP2003502199A 2001-06-04 2002-06-04 組換えタンパク質の高レベルの大規模生成のための組成物および方法 Pending JP2004535189A (ja)

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US29596101P 2001-06-04 2001-06-04
US33362001P 2001-11-26 2001-11-26
US35240402P 2002-01-29 2002-01-29
PCT/US2002/017763 WO2002099089A1 (en) 2001-06-04 2002-06-04 Compositions and methods for high-level, large-scale production of recombinant proteins

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JP2004535189A true JP2004535189A (ja) 2004-11-25
JP2004535189A5 JP2004535189A5 (enExample) 2006-01-05

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US (1) US20040161817A1 (enExample)
EP (1) EP1402006A4 (enExample)
JP (1) JP2004535189A (enExample)
KR (1) KR20040032105A (enExample)
CN (1) CN1533432A (enExample)
AU (1) AU2002310321A1 (enExample)
CA (1) CA2463310A1 (enExample)
WO (2) WO2002099089A1 (enExample)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539781A (ja) * 2005-05-17 2008-11-20 ミリポア・コーポレイション 改良された発現エレメント
JP2008544750A (ja) * 2005-06-30 2008-12-11 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2012515922A (ja) * 2009-01-22 2012-07-12 モメンタ ファーマシューティカルズ インコーポレイテッド ＣＨＯ細胞由来の糖タンパク質産物におけるガラクトース−α−１，３−ガラクトース含有Ｎ−グリカン
WO2019225372A1 (ja) * 2018-05-24 2019-11-28 国立大学法人北海道大学 新規ベクターおよびその利用

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812148B2 (en) 2001-04-05 2010-10-12 Millipore Corporation Vectors comprising CpG islands without position effect varigation and having increased expression
EP1572994B1 (en) 2002-12-20 2007-02-21 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
JP4709743B2 (ja) 2003-02-01 2011-06-22 ミリポア・コーポレイション 改善された発現エレメント
BRPI0408246A (pt) * 2003-03-11 2006-03-01 Applied Research Systems vetores de expressão que compreendem o promotor ie2 do mcmv
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
CN101090968B (zh) 2004-11-08 2011-10-26 科罗迈吉尼科斯公司 高水平表达蛋白质的宿主细胞的选择
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
GB0504587D0 (en) 2005-03-05 2005-04-13 Ml Lab Plc Vectors comprising guinea pig CMV regulatory elements
ES2330784T3 (es) * 2005-03-05 2009-12-15 Millipore Corporation Vectores que comprenden nuevos elementos reguladores.
WO2007107578A1 (en) 2006-03-20 2007-09-27 Chromagenics B.V. Expression augmenting dna fragments, use thereof, and methods for finding thereof
CN105121715B (zh) 2012-12-11 2018-10-26 艾伯特叶史瓦大学爱因斯坦医学院 高通量受体:配体鉴定方法
ES2750550T3 (es) 2013-03-01 2020-03-26 Univ Minnesota Corrección de gen a base de TALEN
CN104341503A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗cd20的人抗体
CN104341505A (zh) * 2013-07-29 2015-02-11 西藏海思科药业集团股份有限公司 对蒙古人种和高加索人种低免疫原性的、抗egfr的人鼠嵌合抗体
SG11201605632SA (en) * 2014-01-21 2016-08-30 Einstein Coll Med Cellular platform for rapid and comprehensive t-cell immunomonitoring
CN109475628A (zh) 2016-05-18 2019-03-15 库尔生物制药有限公司 T细胞调节性多聚体多肽及其使用方法
CA3022331A1 (en) 2016-05-18 2017-11-23 Albert Einstein College Of Medicine, Inc. Variant pd-l1 polypeptides, t-cell modulatory multimeric polypeptides, and methods of use thereof
AU2017379900B2 (en) 2016-12-22 2024-12-05 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
WO2018129474A1 (en) 2017-01-09 2018-07-12 Cue Biopharma, Inc. T-cell modulatory multimeric polypeptides and methods of use thereof
EP3596118B1 (en) 2017-03-15 2024-08-21 Cue Biopharma, Inc. Combination of multimeric fusion polypeptides and immune checkpoint inhibitor for treating hpv-associated cancer
WO2019139896A1 (en) 2018-01-09 2019-07-18 Cue Biopharma, Inc. Multimeric t-cell modulatory polypeptides and methods of use thereof
EP4149534A4 (en) 2020-05-12 2024-09-04 Cue Biopharma, Inc. MULTIMERIC POLYPEPTIDES MODULATING T CELLS AND METHODS OF USE THEREOF
WO2022056014A1 (en) 2020-09-09 2022-03-17 Cue Biopharma, Inc. Mhc class ii t-cell modulatory multimeric polypeptides for treating type 1 diabetes mellitus (t1d) and methods of use thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO162160C (no) * 1987-01-09 1989-11-15 Medi Cult As Serumfritt vekstmedium, samt anvendelse derav.
IL87737A (en) * 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
SE9303601D0 (sv) * 1993-11-01 1993-11-01 Kabi Pharmacia Ab Improved cell cultivation method and medium
WO2000005393A2 (en) * 1998-07-21 2000-02-03 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2008539781A (ja) * 2005-05-17 2008-11-20 ミリポア・コーポレイション 改良された発現エレメント
JP2008544750A (ja) * 2005-06-30 2008-12-11 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2016154537A (ja) * 2005-06-30 2016-09-01 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2019058173A (ja) * 2005-06-30 2019-04-18 オクタフアルマ・アー・ゲー ヒト細胞系における組換えヒトタンパク質の無血清安定トランスフェクションおよび製造
JP2012515922A (ja) * 2009-01-22 2012-07-12 モメンタ ファーマシューティカルズ インコーポレイテッド ＣＨＯ細胞由来の糖タンパク質産物におけるガラクトース−α−１，３−ガラクトース含有Ｎ−グリカン
WO2019225372A1 (ja) * 2018-05-24 2019-11-28 国立大学法人北海道大学 新規ベクターおよびその利用
JPWO2019225372A1 (ja) * 2018-05-24 2021-06-10 国立大学法人北海道大学 新規ベクターおよびその利用
JP7264353B2 (ja) 2018-05-24 2023-04-25 国立大学法人北海道大学 新規ベクターおよびその利用
US11781146B2 (en) 2018-05-24 2023-10-10 National University Corporation Hokkaido University Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
AU2019273200B2 (en) * 2018-05-24 2024-10-31 Fuso Pharmaceutical Industries,Ltd. Novel vector and use thereof

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WO2002099070A3 (en) 2007-11-15
AU2002310321A8 (en) 2008-01-10
EP1402006A4 (en) 2005-11-23
WO2002099070A2 (en) 2002-12-12
CN1533432A (zh) 2004-09-29
EP1402006A1 (en) 2004-03-31
WO2002099089A1 (en) 2002-12-12
KR20040032105A (ko) 2004-04-14
AU2002310321A1 (en) 2002-12-16
US20040161817A1 (en) 2004-08-19
CA2463310A1 (en) 2002-12-12

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