JP2003510249A - ヒトエラスチンおよび他の繊維状タンパク質から誘導される自己アライニング型ペプチド - Google Patents
ヒトエラスチンおよび他の繊維状タンパク質から誘導される自己アライニング型ペプチドInfo
- Publication number
- JP2003510249A JP2003510249A JP2001507072A JP2001507072A JP2003510249A JP 2003510249 A JP2003510249 A JP 2003510249A JP 2001507072 A JP2001507072 A JP 2001507072A JP 2001507072 A JP2001507072 A JP 2001507072A JP 2003510249 A JP2003510249 A JP 2003510249A
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- polypeptide
- sequence shown
- mfu
- elastin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/28—Materials for coating prostheses
- A61L27/34—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L31/00—Materials for other surgical articles, e.g. stents, stent-grafts, shunts, surgical drapes, guide wires, materials for adhesion prevention, occluding devices, surgical gloves, tissue fixation devices
- A61L31/08—Materials for coatings
- A61L31/10—Macromolecular materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/78—Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/70—Biological properties of the composition as a whole
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/86—Products or compounds obtained by genetic engineering
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S977/00—Nanotechnology
- Y10S977/902—Specified use of nanostructure
- Y10S977/904—Specified use of nanostructure for medical, immunological, body treatment, or diagnosis
- Y10S977/915—Therapeutic or pharmaceutical composition
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Epidemiology (AREA)
- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
- Birds (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Vascular Medicine (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Surgery (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Heart & Thoracic Surgery (AREA)
- Oral & Maxillofacial Surgery (AREA)
- Transplantation (AREA)
- Peptides Or Proteins (AREA)
- Materials For Medical Uses (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cosmetics (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/340,736 US6489446B1 (en) | 1996-08-07 | 1999-06-29 | Self-aligning peptides modeled on human elastin and other fibrous proteins |
| US09/340,736 | 1999-06-29 | ||
| PCT/US2000/017829 WO2001000666A2 (en) | 1999-06-29 | 2000-06-29 | Self-aligning peptides derived from human elastin and other fibrous proteins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2003510249A true JP2003510249A (ja) | 2003-03-18 |
| JP2003510249A5 JP2003510249A5 (OSRAM) | 2005-06-02 |
Family
ID=23334714
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2001507072A Pending JP2003510249A (ja) | 1999-06-29 | 2000-06-29 | ヒトエラスチンおよび他の繊維状タンパク質から誘導される自己アライニング型ペプチド |
Country Status (7)
| Country | Link |
|---|---|
| US (2) | US6489446B1 (OSRAM) |
| EP (1) | EP1206492A2 (OSRAM) |
| JP (1) | JP2003510249A (OSRAM) |
| AU (1) | AU5775400A (OSRAM) |
| CA (1) | CA2377229A1 (OSRAM) |
| NZ (1) | NZ516263A (OSRAM) |
| WO (1) | WO2001000666A2 (OSRAM) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008087945A1 (ja) | 2007-01-16 | 2008-07-24 | Prime Polymer Co., Ltd. | 中空成形体用エチレン系樹脂組成物及びそれからなる中空成形体 |
| JP2012126713A (ja) * | 2010-11-25 | 2012-07-05 | Kyushu Institute Of Technology | ペプチドおよびその自己集合方法、その集合体、これらを用いた細胞培養基材、並びに、細胞シートの製造方法 |
Families Citing this family (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPO811797A0 (en) * | 1997-07-18 | 1997-08-14 | University Of Sydney, The | Tropoelastin derivatives |
| US20050204408A1 (en) * | 1998-07-17 | 2005-09-15 | Weiss Anthony S. | Tropoelastin derivatives |
| US6808707B2 (en) * | 2000-02-04 | 2004-10-26 | Matrix Design | Wound healing compositions and methods using tropoelastin and lysyl oxidase |
| US7794490B2 (en) | 2004-06-22 | 2010-09-14 | Boston Scientific Scimed, Inc. | Implantable medical devices with antimicrobial and biodegradable matrices |
| JP5204646B2 (ja) | 2005-04-25 | 2013-06-05 | マサチューセッツ インスティテュート オブ テクノロジー | 止血および他の生理学的活性を促進するための組成物および方法 |
| EP1919938B1 (en) * | 2005-08-29 | 2012-06-06 | AMSilk GmbH | Modified spider silk proteins |
| ES2638242T3 (es) * | 2006-02-16 | 2017-10-19 | Sederma | Polipéptidos KXK y su uso |
| ES2673947T3 (es) | 2006-04-25 | 2018-06-26 | Massachusetts Institute Of Technology | Composiciones y métodos para afectar al movimiento de contaminantes, líquidos corporales u otras entidades y/o afectar a otras condiciones fisiológicas |
| CN100364621C (zh) * | 2006-08-30 | 2008-01-30 | 黄福华 | 人造血管丝素蛋白预凝涂层 |
| CN100364619C (zh) * | 2006-08-30 | 2008-01-30 | 郑军 | 人造血管丝素、胶原蛋白共混预凝涂层 |
| US8574461B2 (en) | 2006-11-03 | 2013-11-05 | Tufts University | Electroactive biopolymer optical and electro-optical devices and method of manufacturing the same |
| CA2704768A1 (en) * | 2006-11-03 | 2008-10-23 | Trustees Of Tufts College | Biopolymer sensor and method of manufacturing the same |
| EP2650112B1 (en) | 2006-11-03 | 2016-08-24 | Trustees Of Tufts College | Nanopatterned biopolymer optical device and method of manufacturing the same |
| CA2669114C (en) * | 2006-11-13 | 2014-12-16 | The University Of Sydney | Use of tropoelastin for repair or restoration of tissue |
| CA2686895A1 (en) * | 2007-05-10 | 2008-11-20 | Elastin Specialties, Inc. | Synthetic peptide materials for joint reconstruction, repair and cushioning |
| EP2244563B1 (en) | 2008-02-01 | 2019-08-28 | Bogin, Robert | Methods, compositions, and systems for production of recombinant spider silk polypeptides |
| US10576123B2 (en) | 2008-10-06 | 2020-03-03 | 3-D Matrix, Ltd. | Tissue occluding agent comprising an IEIKIEIKIEIKI peptide |
| WO2010102337A1 (en) | 2009-03-10 | 2010-09-16 | The University Of Sydney | Injectable biomaterials |
| US9439941B2 (en) | 2009-12-14 | 2016-09-13 | The University Of Hong Kong | Nano cancer barrier device (NCBD) to immobilize and inhibit the division of metastic cancer stem cells |
| US8609621B2 (en) | 2010-11-15 | 2013-12-17 | E I Du Pont De Nemours And Company | Acid-cleavable linkers exhibiting altered rates of acid hydrolysis |
| WO2014008400A2 (en) | 2012-07-06 | 2014-01-09 | 3-D Matrix, Inc. | Fill-finish process for peptide solutions |
| US9688741B2 (en) | 2012-10-23 | 2017-06-27 | Elastagen Pty Ltd | Elastic hydrogel |
| EP3116524B1 (en) | 2014-03-10 | 2018-11-28 | 3-D Matrix Ltd. | Autoassembling peptides for the treatment of pulmonary bulla |
| BR112016020584A2 (pt) | 2014-03-10 | 2017-10-03 | 3 D Matrix Ltd | Composições de peptídeo auto-organizável |
| US10369237B2 (en) | 2014-03-10 | 2019-08-06 | 3-D Matrix, Ltd. | Sterilization and filtration of peptide compositions |
| WO2017120092A1 (en) | 2016-01-06 | 2017-07-13 | 3-D Matrix, Ltd. | Combination compositions |
| CN117085140A (zh) | 2017-12-15 | 2023-11-21 | 立美基股份有限公司 | 表面活性剂肽纳米结构及在药物递送中的用途 |
| CN114569790B (zh) * | 2022-03-14 | 2022-09-02 | 新乡医学院 | 具有促内皮化及抗凝双功能的人工血管及制备方法、用途 |
| CN118064468B (zh) * | 2024-04-12 | 2024-07-23 | 山东兴瑞生物科技有限公司 | 一种重组弹性蛋白、制备方法及其应用 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998005685A2 (en) * | 1996-08-07 | 1998-02-12 | Protein Specialties, Ltd. | Self-aligning peptides derived from elastin and other fibrous proteins |
Family Cites Families (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4132746A (en) | 1976-07-09 | 1979-01-02 | University Of Alabama, Birmingham Medical & Education Foundation | Synthetic elastomeric insoluble cross-linked polypentapeptide |
| NL7713618A (nl) | 1976-12-11 | 1978-06-13 | Bayer Ag | Werkwijze voor de bereiding van een cosmetisch preparaat. |
| DE2705670C3 (de) | 1977-02-11 | 1983-11-17 | Fa. Carl Freudenberg, 6940 Weinheim | Verfahren zur Herstellung von wasserlöslichen Elastin-Hydrolysaten |
| US4419288A (en) | 1981-08-27 | 1983-12-06 | Seton Company | Elastin hydrolyzate |
| US4474851A (en) | 1981-10-02 | 1984-10-02 | The University Of Alabama In Birmingham | Elastomeric composite material comprising a polypeptide |
| US4474763A (en) | 1982-07-07 | 1984-10-02 | Lubowe Irwin I | Skin preparation |
| US4960423A (en) | 1982-11-17 | 1990-10-02 | Smith Donald W | Method of enhancing the attachment of endothelial cells on a matrix and vascular prosthesis with enhanced anti-thrombogenic characteristics |
| US4589882A (en) | 1983-09-19 | 1986-05-20 | Urry Dan W | Enzymatically crosslinked bioelastomers |
| US4659740A (en) | 1985-02-14 | 1987-04-21 | Polydex Pharmaceuticals Ltd. | Cosmetic preparations comprising novel elastin derivatives |
| US5223420A (en) | 1985-03-01 | 1993-06-29 | Institut National De La Sante Et De La Recherche Medicale | Elastin-based product, a procedure for its preparation and its biological applications; in particular as biomaterials and artificial supports |
| JPH0664234B2 (ja) * | 1985-08-29 | 1994-08-22 | 株式会社ニコン | 低倍率投影対物レンズ |
| US4870055A (en) | 1986-04-17 | 1989-09-26 | University Of Alabama At Birmingham | Segmented polypeptide bioelastomers to modulate elastic modulus |
| US5336256A (en) | 1986-04-17 | 1994-08-09 | Uab Research Foundation | Elastomeric polypeptides as vascular prosthetic materials |
| US5250516A (en) | 1986-04-17 | 1993-10-05 | Uab Research Foundation | Bioelastomeric materials suitable for the protection of burn areas or the protection of wound repair sites from the occurrence of adhesions |
| US4783523A (en) | 1986-08-27 | 1988-11-08 | Urry Dan W | Temperature correlated force and structure development of elastin polytetrapeptides and polypentapeptides |
| GB8618374D0 (en) | 1986-07-28 | 1986-09-03 | Hsc Res Dev Corp | Biological vascular prostheses |
| US4979959A (en) | 1986-10-17 | 1990-12-25 | Bio-Metric Systems, Inc. | Biocompatible coating for solid surfaces |
| US5243038A (en) | 1986-11-04 | 1993-09-07 | Protein Polymer Technologies, Inc. | Construction of synthetic DNA and its use in large polypeptide synthesis |
| JPH01501755A (ja) | 1986-11-04 | 1989-06-22 | シントロ コーポレイション | 合成dnaの構成および大ポリペプチドの合成におけるその使用 |
| JP2549118B2 (ja) | 1987-05-30 | 1996-10-30 | 三省製薬株式会社 | 乳化組成物 |
| US5606019A (en) | 1987-10-29 | 1997-02-25 | Protien Polymer Technologies, Inc. | Synthetic protein as implantables |
| IT1219454B (it) | 1988-02-17 | 1990-05-18 | Serono Cesare Ist Ricerca | Metodo di rottura alla metionina di polipeptidi contenenti pontidisolfuro |
| AU630643B2 (en) | 1988-11-09 | 1992-11-05 | Protein Polymers Technologies, Inc. | Functional recombinantly prepared synthetic protein polymer |
| FR2649982B1 (fr) | 1989-07-20 | 1991-09-27 | Inst Nat Sante Rech Med | Membrane biologique artificielle |
| DE3942580A1 (de) * | 1989-12-22 | 1991-06-27 | Basf Ag | Verfahren zur herstellung von hirudin |
| JPH05507276A (ja) | 1990-05-03 | 1993-10-21 | ニュー・イングランド・ディーコネス・ホスピタル・コーポレイション | 動脈造影用合成ペプチド類 |
| CA2095861C (en) | 1990-11-28 | 2002-06-11 | Ronald H. Hoess | Structural proteins from artificial genes |
| CA2151883C (en) | 1992-12-22 | 2009-04-07 | Anthony Steven Weiss | Synthetic tropoelastin |
| US5527610A (en) | 1994-05-20 | 1996-06-18 | The Uab Research Foundation | Elastomeric polypeptide matrices for preventing adhesion of biological materials |
-
1999
- 1999-06-29 US US09/340,736 patent/US6489446B1/en not_active Expired - Fee Related
-
2000
- 2000-06-29 CA CA002377229A patent/CA2377229A1/en not_active Abandoned
- 2000-06-29 JP JP2001507072A patent/JP2003510249A/ja active Pending
- 2000-06-29 WO PCT/US2000/017829 patent/WO2001000666A2/en not_active Ceased
- 2000-06-29 NZ NZ516263A patent/NZ516263A/en not_active IP Right Cessation
- 2000-06-29 EP EP00943258A patent/EP1206492A2/en not_active Withdrawn
- 2000-06-29 AU AU57754/00A patent/AU5775400A/en not_active Abandoned
-
2001
- 2001-09-28 US US09/964,662 patent/US6765086B2/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1998005685A2 (en) * | 1996-08-07 | 1998-02-12 | Protein Specialties, Ltd. | Self-aligning peptides derived from elastin and other fibrous proteins |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008087945A1 (ja) | 2007-01-16 | 2008-07-24 | Prime Polymer Co., Ltd. | 中空成形体用エチレン系樹脂組成物及びそれからなる中空成形体 |
| JP2012126713A (ja) * | 2010-11-25 | 2012-07-05 | Kyushu Institute Of Technology | ペプチドおよびその自己集合方法、その集合体、これらを用いた細胞培養基材、並びに、細胞シートの製造方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US6765086B2 (en) | 2004-07-20 |
| US20030166846A1 (en) | 2003-09-04 |
| EP1206492A2 (en) | 2002-05-22 |
| US6489446B1 (en) | 2002-12-03 |
| AU5775400A (en) | 2001-01-31 |
| WO2001000666A2 (en) | 2001-01-04 |
| WO2001000666A3 (en) | 2001-05-03 |
| CA2377229A1 (en) | 2001-01-04 |
| NZ516263A (en) | 2004-04-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2003510249A (ja) | ヒトエラスチンおよび他の繊維状タンパク質から誘導される自己アライニング型ペプチド | |
| AU728480B2 (en) | Self-aligning peptides derived from elastin and other fibrous proteins | |
| CA2124583C (en) | Synthetic compounds and compositions with enhanced cell binding | |
| US5958428A (en) | Synthetic compounds and compositions with enhanced cell binding | |
| CN1989152A (zh) | 贝类生物粘合剂 | |
| US8673986B2 (en) | Coacervate having an ionic polymer mixed with the adhesive protein of a mussel or of a species of the variome thereof | |
| WO2008150101A2 (en) | Chimeric polypeptide including a mussel adheisve protein and extracellular matrix | |
| GB2485385A (en) | Trimeric fusion protein comprising collagen and a prokaryotic/ viral trimerisation domain | |
| WO2004085606A1 (ja) | 細胞培養基質および細胞接着蛋白質またはペプチドの固相化標品 | |
| US7897727B2 (en) | Bioactive peptide for cell adhesion | |
| WO2007081943A2 (en) | Methods and compositions for promoting attachment of cells of endothelial cell lineage to medical devices | |
| Del Prado Audelo et al. | Recent advances in elastin-based biomaterials | |
| CN116102637B (zh) | 一种稳定的i型重组胶原蛋白及其应用 | |
| Audelo et al. | Recent advances in elastin-based biomaterial | |
| WO2007048115A2 (en) | Method of using and producing tropoelastin and tropoelastin biomaterials | |
| JPH10504551A (ja) | 真核細胞の相互間の接着能力を改変するための細胞接着ペプチド | |
| Ramshaw et al. | Applications of collagen in medical devices | |
| JP5687829B2 (ja) | I型−iv型コラーゲン混成ゲル | |
| US8119598B2 (en) | Synthetic peptide materials for joint reconstruction, repair and cushioning | |
| CN111961137A (zh) | 仿生内皮细胞功能的融合蛋白及其应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20070627 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20100309 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20100526 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20100602 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20100908 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20110426 |
|
| A02 | Decision of refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A02 Effective date: 20111025 |