JP2003342149A - Skin care preparation - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a skin care preparation which has an antioxidizing action and an action for activating dermal fibroblasts and exhibits effects for preventing and improving ageing symptoms such as wrinkles, flabbiness, the deterioration of skin tenseness, and drabness. <P>SOLUTION: This skin care preparation contains a Pemphis plant extract having an antioxidizing action and an action for activating dermal fibroblasts effects for preventing and improving ageing symptoms such as wrinkles, flabbiness, the deterioration of skin tenseness, and drabness. <P>COPYRIGHT: (C)2004,JPO

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin having an excellent effect in preventing or ameliorating skin aging symptoms such as generation of wrinkles and spots and reduction of elasticity of skin, and more specifically, dermal fibroblasts. The present invention relates to a skin external preparation containing a plant extract of the genus Pemphis having a cell activating action and an antioxidant action.

[0002]

2. Description of the Related Art Deterioration of dermal fibroblast function due to aging causes a decrease and degeneration of dermal matrix such as collagen and elastin, which is an important factor for aging symptoms such as wrinkles and deterioration of skin elasticity. . In addition, oxidative damage due to external stress such as ultraviolet rays is also a cause of aging symptoms such as wrinkles, stains, and reduction in skin elasticity. In the field of external preparations for skin up to now, various cell activating agents and antioxidants have been searched and studied in combination in order to prevent or ameliorate such aging symptoms due to functional deterioration of cells and oxidative damage. Examples of the cell activating agent include poncan essence (Japanese Unexamined Patent Publication No. 2001-131045), Genus Ginseng, Heron and extracts thereof (Japanese Unexamined Patent Publication No.
0-178198), a chlorella extract with an organic solvent (JP-A-11-335293), and the like are known as antioxidants, for example, a plant extract of the genus Heteroteca of the Asteraceae (JP-A-11-180886) and Cayuangin. (Japanese Patent Application Laid-Open No. 10-182413) and the like are known.

However, the already reported physiologically active substances often act only on a part of the process of aging phenomenon, which is insufficient as an essential improving effect. Further, when blended in the base of an external preparation for skin, it is necessary to blend a considerably high concentration in order to obtain an effective effect, which may impart an undesired color or odor to the formulation. At present, there are few things that can satisfy all of the effects and stability.

[0004]

Therefore, in the present invention, it has a dermal fibroblast activating action and an antioxidant action,
It is an object of the present invention to provide an external preparation for skin which is excellent in preventing and improving skin aging symptoms.

[0005]

[Means for Solving the Problems] In order to find an active ingredient excellent in prevention and improvement of skin aging symptoms, the present inventors have
The dermal fibroblast activation and antioxidant effects of various substances were investigated. As a result, the genus Mizuganpi ( P
The present invention has been completed by finding excellent dermal fibroblast activating action and antioxidant action in plant extracts.

[0006]

DETAILED DESCRIPTION OF THE INVENTION Mizuganpi genus (Pemphis)
The plant is a plant of the family Liliaceae, widely distributed in India, Malaysia, and the Pacific Islands. Genus Mizugumpi ( Pe
mphis ) plants include the mizugumpi ( Pemphi)
s acidula Forst. ), Madagascar Ms. Ganpi (Pemphis madagascarie
nsis (Bak.) H. Perr.) And the like are known.

These genus Pemphis
When using plants, it is common to use extracts. For the extraction, any part of the stem, branch, fruit, leaf, flower, seed, bark, sap, root, bud, etc. of the genus Pemphis may be used. Use leaves and seeds. When extracting, it may be used as it is, but in consideration of extraction efficiency, it is preferable to perform extraction after performing processes such as shredding, drying and crushing. The extraction can also be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or subcritical fluid. In order to improve extraction efficiency, stirring or homogenization in an extraction solvent may be performed. As the extraction temperature, it is appropriate to set the temperature to about 5 ° C. to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 1 hour to 14 days.

As the extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, and esters such as ethyl acetate and butyl acetate. Solvents such as ketones such as acetone, acetone, and ethyl methyl ketone can be used, and one or more selected from these solvents are used. Also, saline, phosphate buffer,
Phosphate buffered saline may be used. Further, one or more supercritical fluids or subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol and ammonia may be used.

The extract of the Pemphis plant with the above solvent can be used as it is, but the concentrated and dried product is redissolved in water or a polar solvent, or its physiological action is impaired. It may be used after purification treatment such as decolorization, deodorization, and desalting within a range not present, or after fractionation treatment by column chromatography or the like. The above-mentioned extract of the Pemphis plant and its treated products and fractions can be freeze-dried after each treatment and fractionation, and dissolved in a solvent before use. It can also be used by encapsulating it in vesicles such as liposomes or in microcapsules.

In the present invention, the genus Mizugumpi ( Pem
By adding a phis ) plant extract to a skin external preparation, it exerts an excellent effect in preventing and improving aging symptoms such as wrinkles, tarmi, skin firmness and dullness.

The external preparation for skin having a dermal fibroblast activating action and an antioxidant action according to the present invention is a genus Mizugumpi ( Pe
mphis ) plant extract as an active ingredient.

[0012] Mizuganpi genus in the present invention (Pemph
is ) The blending amount of the plant extract can be adjusted depending on the type and purpose of the skin external preparation, but is preferably 0.0001 to 10.0% by weight based on the total amount of the skin external preparation,
More preferably, it is 0.001 to 5.0% by weight.

The external preparation for skin according to the present invention is a lotion,
It can be provided in various dosage forms such as emulsion, gel, cream, ointment, powder and granules.

The external preparation for skin according to the present invention is compounded in ordinary pharmaceutical products, quasi-drugs, skin cosmetics, hair cosmetics and cleansers in addition to the plant extract of the genus Pemphis. , Oily ingredients, moisturizers, powders, pigments, emulsifiers, solubilizers, detergents, UV absorbers, thickeners, drugs,
Fragrances, resins, antibacterial and antifungal agents, alcohols and the like can be appropriately mixed. Further, it may be used in combination with other cell activators, antioxidants and plant extracts as long as the effects of the present invention are not impaired.

[0015]

EXAMPLES The features of the present invention will be described in more detail with reference to examples. First, the genus Mizugumpi of the present invention ( Pem
phis ) A method for preparing a plant extract will be described.

[Preparation Method 1] Genus Mizugumpi ( Pemph)
is ) 10 liters of a 50% by weight aqueous ethanol solution was added to 1 kg of the dried and pulverized plant material, and the mixture was immersed at room temperature for 7 days. The extract was filtered and collected, and the solvent was removed to obtain a Pemphis plant extract.

[Preparation Method 2] Genus Mizugumpi ( Pemph)
is ) Add 9 liters of water to 1 kg of dried and ground plant,
Reflux for 6 hours at 90 ° C. for extraction. The extract was recovered by filtration, after removal of the solvent, Mizuganpi genus (Pemph
is ) A plant extract was obtained.

[Preparation Method 3] Genus Mizugumpi ( Pemph)
is ) To 1 kg of the dried and pulverized plant material, 9 liters of methanol was added and immersed at room temperature for 7 days. The extract was recovered by filtration, after removal of the solvent, Mizuganpi genus (Pemphi
s ) A plant extract was obtained.

[Preparation Method 4] Plants of the genus Pemphis were put into a supercritical extraction apparatus and extracted with a supercritical fluid of carbon dioxide at 40 ° C. under an atmospheric pressure of 15 MPa. The extract was collected, and the genus Mizugumpi ( Pemp
his ) plant extract was obtained.

Next, the activating effect on the dermal fibroblasts of the Pemphis plant extract will be shown.

The evaluation was carried out by the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. As the seeding medium, Dulbecco's modified Eagle medium (DMEM) supplemented with 1% fetal bovine serum was used. After culturing for 24 hours, the culture medium was replaced with a test medium supplemented with a Pemphis plant extract, and further cultivated for 48 hours. Next, add 3- (4,5-dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) to 400 μm.
The medium was replaced with a medium containing g / mL, and the cells were cultured for 2 hours. The formazan generated by the ring opening of the tetrazolium ring was extracted with 2-propanol, and the cells were mixed with a microplate reader 5
Absorbance at 50 nm was measured. At the same time turbidity is 650
The absorbance at nm was measured, and the cell activation effect was evaluated by the difference between both measured values. Table 1 shows the evaluation results of the extract obtained by the preparation method 1 from the mixed part of leaves and branches of Mizuganpi, in which the cell activation action when the extract was not added was 100.

[0022]

[Table 1]

From Table 1, it can be seen that in the extract of Mizuganpi, significant addition of 0.25 to 1.0 mg / mL of Mizugunpi extract resulted in significant fibroblasts with a risk rate of less than 1% as compared with the case of no addition. The activating effect of was confirmed. From this, it was revealed that the plant extract of the genus Pemphis has an excellent fibroblast activating effect.

Next, the antioxidant effect of the extract of Pemphis plant will be shown.

The evaluation was carried out by the following procedure. 50% by weight
100 µL of a Mizuganpi extract solution diluted to a concentration of 0.1 mg / mL with an aqueous ethanol solution was added to a 96-well microplate as a sample. Next, 100 μL of a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was added to a 96-well microplate. The mixture was left in the dark with stirring, and the absorbance at 516 nm was measured after 10 minutes and 17 hours. When the absorbance when the sample was not added was (A) and the absorbance when the sample was added was (B), the value of the formula (1) was defined as the radical scavenging rate. Formula (1) {1- (B) / (A)} × 100 (%) Table 2 shows the experimental results of the extract obtained by the preparation method 1 from the mixed part of leaves and branches of Mizuganpi.

[0026]

[Table 2]

As is clear from Table 2, it was found that the plant extract of the genus Pemphis has an excellent antioxidant effect.

Then, the genus Mizugumpi ( Pe according to the present invention)
mphis ) The formulation of the example which mix | blended the plant extract is shown, but the Mizuganpi extract and Madagascar Mizuganpi extract which were mix | blended with the formulation in Examples 7-19 are extracts from the mixing part of a leaf and a branch. .

Examples 1 to 6 Emulsion (1) Squalane 10.0 (% by weight) (2) Methylphenylpolysiloxane 4.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phosphorus Lipid 0.1 (5) Polyoxyethylene sorbitan monostearate (20 EO) 1.3 (6) Sorbitan monostearate 1.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9) Carboxyvinyl polymer 0.15 (10) Purified water 50.85 (11) Arginine (1% by weight aqueous solution) 20.0 (12) Orchugi plant extract 2.0 shown in Table 3 Production method: (1) The oil phase components (6) to (6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After the emulsification is completed, cooling is started, and (11) and (12) are added in order and mixed uniformly.

[0030]

[Table 3]

[Example 7] Lotion (1) Ethanol 15.0 (wt%) (2) Polyoxyethylene (40EO) hydrogenated castor oil 0.3 (3) Perfume 0.1 (4) Purification Water 81.36 (5) Citric acid 0.02 (6) Sodium citrate 0.1 (7) Glycerin 3.0 (8) Hydroxyethyl cellulose 0.1 (9) Mizuganpi extract [Preparation method 3] 0.02 Manufacturing method: (2) and (3) are dissolved in (1). After dissolution
After sequentially adding (4) to (8), thoroughly stir,
Add (9) and mix evenly.

[Example 8] Cream (1) Squalane 10.0 (wt%) (2) Stearic acid 2.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0.1 (5) Cetanol 3.6 (6) Lipophilic type glyceryl monostearate 2.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9) Arginine (20% by weight aqueous solution) 15.0 (10) Purified water 40.7 (11) Carboxyvinyl polymer (1% by weight aqueous solution) 15.0 (12) Mizuganpi extract [Preparation method 1] 1.0 Production method: Oil phase components of (1) to (6) Is dissolved by heating at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After completion of emulsification, (1
Add 1), start cooling, add (12) at 40 ° C,
Mix evenly.

[Example 9] Beauty liquid (1) Purified water 32.35 (wt%) (2) Glycerin 10.0 (3) Sucrose fatty acid ester 1.3 (4) Carboxyvinyl polymer (1 wt% aqueous solution) ) 17.5 (5) Sodium alginate (1% by weight aqueous solution) 15.0 (6) Polyglyceryl monolaurate 1.0 (7) Macadamia nut oil fatty acid phytosteryl 3.0 (8) N-lauroyl-L-glutamate di (phytosteryl) -2-octyldodecyl) 2.0 (9) hydrogenated palm oil 2.0 (10) squalane (derived from olive) 1.0 (11) behenyl alcohol 0.75 (12) beeswax 1.0 (13) jojoba oil 1. 0 (14) 1,3-butylene glycol 10.0 (15) L-arginine (10% by weight aqueous solution) 2.0 (16) Mizuganpi extract [Preparation method 3] 0 Process 1: (1) mixing the aqueous phase component to (6), dissolved by heating at 75 ° C.. On the other hand, the oil phase components (7) to (14) are mixed and heated and dissolved at 75 ° C. Next, the oil phase component is added to the above water phase component to carry out preliminary emulsification, and then uniformly emulsified with a homomixer. Start cooling after the emulsification is completed, and
Add (15) at ℃. Further cool to 40 ℃, (1
Add 6) and mix evenly.

Example 10 Aqueous Gel (1) Carboxyvinyl Polymer 0.5 (wt%) (2) Purified Water 86.1 (3) Sodium Hydroxide (10 wt% Aqueous Solution) 0.5 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Perfume 0.1 (7) Mizuganpi extract [Preparation method 4] 0.2 (8) Polyoxyethylene (60 EO) hydrogenated castor oil 0 .1 (9) Glycerin 2.0 (10) Mizuganpi Extract [Preparation Method 3] 0.2 (11) Madagascar Mizugunpi Extract [Preparation Method 1] 0.2 Preparation Method: (1) was added to (2) , After stirring evenly,
Add (3). After stirring uniformly, (5) dissolved in advance in (4) is added. After uniformly stirring, (6) to (8) that have been mixed in advance are added. After stirring evenly,
(9) to (11) are sequentially added and mixed uniformly.

[Example 11] Cleansing agent (1) Squalane 81.95 (2) Polyoxyethylene glyceryl isostearate 15.0 (3) Purified water 3.0 (4) Mizuganpi extract [Preparation method 4] 02 (5) Madagascar mizugumpi extract [Preparation method 4] 0.03 Production method: (1) and (2) are uniformly dissolved. to this,
(3) to (5) are sequentially added and mixed uniformly.

[Example 12] Facial cleansing foam (1) Stearic acid 16.0 (% by weight) (2) Myristic acid 16.0 (3) Lipophilic glyceryl monostearate 2.0 (4) Glycerin 20.0 (5) Sodium hydroxide 7.5 (6) Coconut oil fatty acid amide propyl betaine 1.0 (7) Purified water 36.5 (8) Mizuganpi extract [Preparation method 3] 0.5 (9) Madagascar Mizuganpi extract [Preparation method 2] 0.5 Manufacturing method: The oil phase components of (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and uniformly mixed with the oil phase component and stirred. Start cooling, add (8) and (9) at 40 ° C., and mix uniformly.

Example 13 Makeup Base Cream (1) Squalane 10.0 (wt%) (2) Cetanol 2.0 (3) Glycerin Tri-2-ethylhexanoate 2.5 (4) Lipophilic Glyceryl monostearate 1.0 (5) Propylene glycol 11.0 (6) Sucrose fatty acid ester 1.3 (7) Purified water 70.4 (8) Titanium oxide 1.0 (9) Red iron oxide 0.1 ( 10) Yellow iron oxide 0.4 (11) Perfume 0.1 (12) Mizuganpi extract [Preparation method 2] 0.1 (13) Madagascar Mizugunpi extract [Preparation method 1] 0.1 Manufacturing method: (1) The oil phase components (4) to (4) are mixed and heated to dissolve at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, heated and dissolved at 75 ° C., the pigments (8) to (10) are added thereto, and the components are uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component and emulsified with a homomixer. After the emulsification is completed, cooling is started, and at 40 ° C (11) ~
Add the component (13) and mix evenly.

Example 14 Emulsion Foundation (1) Methylpolysiloxane 2.0 (wt%) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5 ) Polyoxyethylene (20EO) sorbitan monostearate 1.3 (6) sorbitan monostearate 0.7 (7) 1,3-butylene glycol 8.0 (8) xanthan gum 0.1 (9) Methyl paraoxybenzoate 0.1 (10) Purified water 58.52 (11) Titanium oxide 9.0 (12) Talc 7.4 (13) Red iron oxide 0.5 (14) Yellow iron oxide 1.1 (15) Black Iron oxide 0.1 (16) Perfume 0.1 (17) Mizuganpi extract [Preparation method 4] 0.04 (18) Madagascar Mizuganpi extract [Preparation method 3] 0.04 Production method: (1) to (6) )of The phase components were mixed, heated and dissolved at 75 ° C.. On the other hand, the aqueous phase components (7) to (10) are mixed, heated and dissolved at 75 ° C., the pigments (11) to (15) are added thereto, and the components are uniformly dispersed with a homomixer. Add the oil phase component and emulsify. Cooling is started after the emulsification is completed, and 40
The components (16) to (18) are sequentially added at ℃ and mixed uniformly.

[Example 15] Water-in-oil type emollient cream (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglycerin oleic acid ester 5.0 (5) Sodium chloride 1.3 (6) Potassium chloride 0.1 (7) Propylene glycol 3.0 (8) 1,3-Butylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 ( 10) Mizuganpi extract [Preparation method 1] 0.5 (11) Purified water 45.9 (12) Perfume 0.1 (13) Madagascar Mizugunpi extract [Preparation method 4] 2.0 Manufacturing method: (5) Dissolve (6) in a part of (11), 50 ℃
And heated gradually to (4) heated to 50 ° C. with stirring. After mixing this, it melt | dissolves by heating at 70 degreeC and it disperses uniformly in (1)-(3). To this (7) ~ (1
What was heated and dissolved at 70 ° C. was added to the rest of (11) with stirring, and the mixture was emulsified with a homomixer. Start cooling after the emulsification, add (12) and (13) at 40 ° C,
Mix evenly.

[0040]   [Example 16] Heart nick (1) Ethanol 50.0 (wt%) (2) Purified water 49.898 (3) Mizuganpi Extract [Preparation Method 3] 0.001 (4) Madagascar mizugumpi extract [Preparation method 4] 0.001 (5) Perfume 0.1 Manufacturing method: Components (1) to (5) are mixed and homogenized.

Example 17 Pack (1) Purified water 68.9 (% by weight) (2) Polyvinyl alcohol 12.0 (3) Ethanol 10.0 (4) Glycerin 5.0 (5) Polyethylene glycol (average) Molecular weight 1000) 2.0 (6) Mizugumpi extract [Preparation method 1] 1.0 (7) Madagascar Mizugumpi extract [Preparation method 2] 1.0 (8) Perfume 0.1 Manufacturing method: (2) and ( After mixing 3) and heating to 80 ° C,
It is dissolved in (1) heated to 80 ° C. After uniformly dissolving, (4) to (5) are added, and cooling is started while stirring. Cool to 40 ° C., add (6) to (8), and mix uniformly.

[Example 18] Hair wax (1) Stearic acid 3.0 (wt%) (2) Microcrystalline wax 2.0 (3) Cetyl alcohol 3.0 (4) Highly polymerized methylpolysiloxane 2.0 (5) Methyl polysiloxane 5.0 (6) Poly (oxyethylene / oxypropylene) methyl polysiloxane copolymer 1.0 (7) Methyl paraoxybenzoate 0.1 (8) 1,3-butylene glycol 7. 5 (9) Arginine 0.7 (10) Purified water 71.6 (11) Mizuganpi Extract [Preparation Method 1] 2.0 (12) Madagascar Mizugunpi Extract [Preparation Method 1] 2.0 (13) Fragrance 0.1 Manufacturing method: The oil phase components of (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the water phase components of (7) to (10) are
It is heated and dissolved at 5 ° C., the above oil phase component is added, and the mixture is emulsified with a homomixer. After completion of the emulsification, cooling is started, and the components (11) to (13) are added at 40 ° C. and mixed uniformly.

[0043]   [Example 19] Bath salt (1) Fragrance 0.3 (wt%) (2) Mizuganpi Extract [Preparation Method 1] 0.5 (3) Madagascar mizugumpi extract [Preparation method 3] 0.5 (4) Sodium hydrogen carbonate 50.0 (5) Sodium sulfate 48.7 Manufacturing method: (1) to (5) are uniformly mixed.

A use test was conducted on Examples 1 to 10 of the present invention to evaluate the effect of improving wrinkles, tarmi, firmness of skin, and dullness. At that time, in Example 1, the blended Mizugumpi extract was replaced with purified water, and a use test was simultaneously performed as Comparative Example 1.

For each sample, 20 male and female panelists in their 50's to 60's, in which aging symptoms such as wrinkles, tarmi, reduction of skin firmness, and dullness were noticeably observed, were used blindly for 1 month before and after use. The change in the skin condition was observed and evaluated. As an index of skin aging symptoms, wrinkles,
About Talmi, skin firmness, and dullness, "improvement",
The evaluation was made in three stages of "slightly improved" and "no change", and Table 4 shows the number of panelists who obtained each evaluation.

[0046]

[Table 4]

From Table 4, it was found that wrinkles, tarmi, skin firmness, and dullness were not observed in more than half of the panelists in the group of Comparative Example using no Pemphis plant extract. In the group used in the example in which the extract of the genus Pemphis was mixed, a clear improvement was observed in 60% or more of the panelists.

As described above, the examples of the present invention were more effective than the conventional comparative examples in improving skin aging symptoms such as wrinkles, tarmi, skin firmness, and dullness.

[0049]

INDUSTRIAL APPLICABILITY As described in detail above, according to the present invention, it has a fibroblast activating action and an antioxidant action, and prevents or improves aging symptoms such as wrinkles, tarmi, skin firmness and dullness. It was possible to obtain an external preparation for skin that exhibits excellent effects.

─────────────────────────────────────────────────── ─── Continuation of front page (51) Int.Cl. ⁷ Identification code FI theme code (reference) A61P 43/00 107 A61P 43/00 107 F term (reference) 4C083 AA082 AA111 AA112 AA122 AB032 AB232 AB242 AB312 AB332 AB352 AB432 AC012 AC022 AC072 AC102 AC122 AC242 AC302 AC352 AC422 AC432 AC442 AC482 AC582 AC712 AD042 AD092 AD112 AD152 AD162 AD222 AD282 AD302 AD352 AD492 AD572 BB47 BB51 CC02 CC04 CC05 CC07 CC11 CC12 CC22 CC23 CC25 CC33 DD08 DD17 DD22 DD23C27 CC25 CC33 DD08 DD17 DD22 DD23C27 CC12 CC12 AC05 BA08 BA09 BA10 CA03 NA14 ZA89 ZB21 ZB22

Claims (4)

[Claims] 1. An external preparation for skin, comprising an extract of Pemphis plant. 2. The external preparation for skin according to claim 1, which has an effect of preventing and improving aging symptoms. 3. The external preparation for skin according to claim 1, which has a dermal fibroblast activation effect. 4. The external preparation for skin according to claim 1, which has an antioxidant effect.