JP3429297B1 - External preparation for skin - Google Patents
External preparation for skinInfo
- Publication number
- JP3429297B1 JP3429297B1 JP2002043904A JP2002043904A JP3429297B1 JP 3429297 B1 JP3429297 B1 JP 3429297B1 JP 2002043904 A JP2002043904 A JP 2002043904A JP 2002043904 A JP2002043904 A JP 2002043904A JP 3429297 B1 JP3429297 B1 JP 3429297B1
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- extract
- skin
- livistona
- genus
- external preparation
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Abstract
【要約】
【課題】 真皮線維芽細胞の賦活作用と抗酸
化作用とを有し、シワ、タルミ、肌のハリの低下、及び
クスミなどの老化症状の予防や改善に優れた効果を発揮
する皮膚外用剤を提供する。
【解決手段】 シワ、タルミ、肌のハリの低下、
及びクスミなどの老化症状の予防や改善のために、皮膚
外用剤に真皮線維芽細胞の賦活作用と抗酸化作用とを有
するビロウ属(Livistona R. Br.)植物
抽出物を配合する。Kind Code: A1 The skin has an activating effect on dermal fibroblasts and an antioxidant effect, and exhibits excellent effects for preventing and improving aging symptoms such as wrinkles, tarmi, skin firmness, and dark spots. Provide external preparations. [Solution] Wrinkles, tarumi, decrease in skin firmness,
In order to prevent or improve aging symptoms such as scabs and the like, an external preparation for skin is mixed with a plant extract of the genus Livistona ( Livistona R. Br.) Having an activating action of dermal fibroblasts and an antioxidant action.
Description
【0001】[0001]
【発明の属する技術分野】本発明は、シワやシミの発
生、皮膚の弾性の低下といった皮膚の老化症状の防止或
いは改善において、優れた効果を有する皮膚外用剤に関
し、さらに詳しくは、真皮線維芽細胞賦活作用と抗酸化
作用を有するビロウ属(LivistonaR. B
r.)植物抽出物を配合した皮膚外用剤に関する。TECHNICAL FIELD The present invention relates to an external preparation for skin having an excellent effect in preventing or ameliorating skin aging symptoms such as generation of wrinkles and spots and reduction of elasticity of skin, and more specifically, dermal fibroblasts. The genus Virowa ( Livistona R. B.) having a cell activating action and an antioxidant action
r.) It relates to a skin external preparation containing a plant extract.
【0002】[0002]
【従来の技術】紫外線等の外来ストレス等の酸化障害に
より、シワやシミの発生、皮膚の弾性の低下といった皮
膚の老化現象が生じることが知られている。また、乾燥
や皮膚の真皮線維芽細胞の機能低下や、コラーゲン、エ
ラスチン等の真皮マトリックスの減少,変性も老化現象
の重要な要因となっている。これまでの皮膚外用剤の分
野では、かかる皮膚の老化を防止するために、抗酸化剤
や細胞賦活剤、コラーゲン産生促進剤やエラスターゼ活
性阻害剤といった種々の生理活性物質の検索及び配合検
討がなされてきた。例えば、抗酸化剤としては、キク科
へテロテカ属植物抽出物(特開平11−180886)
が知られており、真皮線維芽細胞賦活剤としては、ビワ
抽出物(特公平5−17206)やコラーゲン又はゼラ
チンのコラゲナーゼによる分解物(特開2000−30
9521)などが知られている。また、コラーゲン産生
促進剤としては、モモの種子の抽出物(特開平11−3
35235)、ブナ科ブナ植物の木の芽からの抽出物
(特開平10−203952)などが知られている。エ
ラスターゼ活性阻害剤としては、植物抽出物とグルタチ
オンとの併用(特開平11−263720)、チャノキ
の溶媒抽出物(特開平11−246388)などが知ら
れている。2. Description of the Related Art It is known that skin aging phenomena such as generation of wrinkles and spots and reduction of elasticity of skin occur due to oxidative damage such as external stress such as ultraviolet rays. In addition, dryness and functional deterioration of dermal fibroblasts, and reduction and degeneration of dermal matrix such as collagen and elastin are also important factors in the aging phenomenon. In the field of external skin preparations to date, in order to prevent such skin aging, various physiologically active substances such as antioxidants, cell activating agents, collagen production promoters, and elastase activity inhibitors have been searched and studied for compounding. Came. For example, as an antioxidant, an extract of a plant belonging to the genus Heterotheca (Asteraceae) (JP-A-11-180886)
Are known, and examples of the dermal fibroblast activator include loquat extract (Japanese Patent Publication No. 5-17206) and a degradation product of collagen or gelatin with collagenase (JP-A-2000-30).
9521) and the like are known. Further, as a collagen production promoter, peach seed extract (JP-A-11-3
35235), an extract from a tree bud of a beech plant of the family Beech (Japanese Unexamined Patent Publication No. 10-203952), and the like are known. As an elastase activity inhibitor, a combination of a plant extract and glutathione (JP-A-11-263720), a solvent extract of tea tree (JP-A-11-246388) and the like are known.
【0003】しかし、すでに報告されている生理活性物
質の中には、作用効果が不十分であるため、皮膚外用剤
の基剤中に配合した場合、有効な効果を得るにはかなり
の高濃度を配合しなければならず、製剤に好ましくない
色や臭いを付与してしまうなど、作用効果や安定性の面
ですべてを満足できるものが少ないのが現状であった。However, among the physiologically active substances that have already been reported, the action and effect are insufficient, and therefore, when incorporated into the base of the skin external preparation, a considerably high concentration is required to obtain an effective effect. However, the present situation is that there are few that can satisfy all of the effects and stability, such as imparting an unfavorable color or odor to the preparation.
【0004】[0004]
【発明が解決しようとする課題】そこで、本発明におい
ては、真皮線維芽細胞の賦活作用と抗酸化作用とを有
し、皮膚の老化症状の予防や改善効果に優れた皮膚外用
剤を提供することを目的とした。Therefore, in the present invention, there is provided a skin external preparation which has a dermal fibroblast activating action and an antioxidant action and is excellent in the prevention and amelioration effect of skin aging symptoms. It was intended.
【0005】[0005]
【課題を解決するための手段】本発明者らは以上のよう
な現況に鑑み、広く種々の物質について真皮線維芽細胞
の賦活作用と抗酸化作用に基づく皮膚の老化症状の予防
や改善効果について検討を行った。その結果、ビロウ属
(Livistona R. Br.)植物抽出物に優れ
た真皮線維芽細胞の賦活作用と抗酸化作用を見出し、本
発明を完成するに至った。SUMMARY OF THE INVENTION In view of the above-mentioned circumstances, the present inventors have investigated a wide variety of substances for the prevention and improvement of skin aging symptoms based on the dermal fibroblast activation and antioxidant effects. Study was carried out. As a result, they have found an excellent dermal fibroblast activating action and antioxidant action in a plant extract of the genus Livistona R. Br. And have completed the present invention.
【0006】[0006]
【発明の実施の形態】ビロウ属(Livistona
R. Br.)植物は、亜熱帯地域に広く分布しているヤ
シ科の植物である。日本近郊では、中国、台湾北部から
南西諸島を経て、九州から四国の南部まで分布してい
る。海岸近くに生育し、扇形葉をもつ常緑の高木であ
る。ビロウ属(Livistona R. Br.)植物
としては、ビロウ(Livistona chinen
sis (Jacq.) R. Br.var. subg
lobosa (Hassk.) Becc.)、トウビ
ロウ(Livistona chinensis R.
Br. var. chinensis)、ジャワビロ
ウ(Livistona rotundifolia
Mart.)、オーストラリアビロウ(Livist o
na australis)、オガサワラビロウ(Li
vistona chinensis R.Br. va
r. boninensis Becc.)などが知ら
れている。BEST MODE FOR CARRYING OUT THE INVENTION
R. Br.) Plant is a plant of the family Palmidae widely distributed in the subtropical region. In the suburbs of Japan, it is distributed from China and northern Taiwan through the Nansei Islands to Kyushu and the southern part of Shikoku. It is an evergreen tree that grows near the coast and has fan-shaped leaves. Examples of plants belonging to the genus ( Livistona R. Br.) Include:
sis (Jacq.) R. Br. var. subg
lobosa (Hassk.) Becc.), Toubirou (Livistona chinensis R.
Br. Var. chinensis ), Javabill ( Livistona rotundifolia)
Mart.), Australia Below (Livist o
na australis), Ogasawarabiro (Li
vistona chinensis R. Br. va
r. Boninensis Becc. ) Is known.
【0007】これらビロウ属(Livistona
R. Br.)植物を使用する際は、抽出物を用いるのが
一般的である。抽出には、ビロウ属(Liviston
a R. Br.)植物の幹、枝、果実、葉、花、種子、
樹皮、樹液、根、芽などのいずれの部位を用いても構わ
ないが、簡便に利用するには、葉や果実を用いるとよ
い。抽出の際は、生のまま用いてもよいが、抽出効率を
考えると、細切,乾燥,粉砕等の処理を行った後に抽出
を行うことが好ましい。抽出は、抽出溶媒に浸漬する
か、超臨界流体や亜臨界流体を用いた抽出方法でも行う
ことができる。抽出効率を上げるため、撹拌や抽出溶媒
中でホモジナイズしてもよい。抽出温度としては、5℃
程度から抽出溶媒の沸点以下の温度とするのが適切であ
る。抽出時間は抽出溶媒の種類や抽出温度によっても異
なるが、1時間〜14日間程度とするのが適切である。[0007] These Below genera (Livistona
When using R. Br.) Plants, it is common to use extracts. For extraction, the genus Liviston
a R. Br.) plant trunks, branches, fruits, leaves, flowers, seeds,
Although any part such as bark, sap, root, bud and the like may be used, it is preferable to use leaves or fruits for easy use. When extracting, it may be used as it is, but in consideration of extraction efficiency, it is preferable to perform extraction after performing processes such as shredding, drying and crushing. The extraction can also be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or subcritical fluid. In order to improve extraction efficiency, stirring or homogenization in an extraction solvent may be performed. Extraction temperature is 5 ℃
It is appropriate to adjust the temperature to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 1 hour to 14 days.
【0008】抽出溶媒としては、水の他、メタノール,
エタノール,プロパノール,イソプロパノール等の低級
アルコール、1,3−ブチレングリコール,プロピレン
グリコール,ジプロピレングリコール,グリセリン等の
多価アルコール、エチルエーテル,プロピルエーテル等
のエーテル類、酢酸エチル,酢酸ブチル等のエステル
類、アセトン,エチルメチルケトン等のケトン類などの
極性有機溶媒を用いることができ、これらより1種又は
2種以上を選択して用いる。また、生理食塩水,リン酸
緩衝液,リン酸緩衝生理食塩水等を用いてもよい。さら
に、水や二酸化炭素、エチレン、プロピレン、エタノー
ル、メタノール、アンモニアなどの1種又は2種以上の
超臨界流体や亜臨界流体を用いてもよい。As the extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, and esters such as ethyl acetate and butyl acetate. It is possible to use polar organic solvents such as acetone, ketones such as ethyl methyl ketone, etc., and one kind or two or more kinds are selected from these and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used. Further, one or more supercritical fluids or subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol and ammonia may be used.
【0009】ビロウ属(Livistona R. B
r.)植物の上記溶媒による抽出物は、そのままでも使
用することができるが、濃縮,乾固した物を水や極性溶
媒に再度溶解したり、或いはこれらの生理作用を損なわ
ない範囲で脱色,脱臭,脱塩等の精製処理を行ったり、
カラムクロマトグラフィー等による分画処理を行った後
に用いてもよい。ビロウ属(Livistona R.
Br.)植物の前記抽出物やその処理物及び分画物は、
各処理及び分画の後凍結乾燥し、用時に溶媒に溶解して
用いることもできる。また、リポソーム等のベシクルや
マイクロカプセル等に内包させて用いることもできる。The genus Birowa ( Livistona R. B)
r.) The extract of the plant with the above solvent can be used as it is, but the concentrated and dried product is redissolved in water or a polar solvent, or decolorized within a range not impairing these physiological actions, Purification processing such as deodorization and desalination,
You may use it, after performing a fractionation process by column chromatography etc. The genus Billow ( Livistona R.
Br.) The above-mentioned extracts of plants, their processed products and fractions,
It is also possible to freeze-dry after each treatment and fractionation and dissolve in a solvent before use. It can also be used by encapsulating it in vesicles such as liposomes or in microcapsules.
【0010】本発明においては、ビロウ属(Livis
tona R. Br.)植物抽出物を皮膚外用剤に配合
することにより、シワ、タルミ、肌のハリの低下、及び
クスミ等の老化症状の予防や改善に優れた効果を発揮す
る。In the present invention, the genus Livis ( Livis
Tona R. Br.) plant extract, when added to a skin external preparation, exerts an excellent effect in preventing and improving aging symptoms such as wrinkles, tarmi, skin firmness and dullness.
【0011】本発明における真皮線維芽細胞賦活作用と
抗酸化作用を有する皮膚外用剤は、ビロウ属(Livi
stona R. Br.)植物抽出物を有効成分とす
る。The external preparation for skin having a dermal fibroblast activating action and an antioxidant action according to the present invention is a genus of the genus Virou ( Liv.
stona R. Br.) plant extract as an active ingredient.
【0012】本発明におけるビロウ属(Livisto
na R. Br.)植物抽出物の配合量は、皮膚外用剤
の種類や目的等によって調整することができるが、好適
な配合量は、皮膚外用剤の全量に対して、0.0001
〜10.0重量%である。The genus Livisto in the present invention
na R. Br.) The amount of the plant extract can be adjusted according to the type and purpose of the skin external preparation, but a suitable amount is 0.0001 based on the total amount of the skin external preparation.
~ 10.0% by weight.
【0013】本発明に係る皮膚外用剤は、ローション,
乳液,ゲル,クリーム,軟膏剤,粉末,顆粒等、種々の
剤型で提供することができる。The external preparation for skin according to the present invention is a lotion,
It can be provided in various dosage forms such as emulsion, gel, cream, ointment, powder and granules.
【0014】なお、本発明に係る皮膚外用剤には、ビロ
ウ属(Livistona R.Br.)植物抽出物の他
に、油性成分,界面活性剤,保湿剤,顔料,紫外線吸収
剤,抗酸化剤,香料,防菌防黴剤等の一般的な医薬品及
び化粧料用原料や、生理活性成分を含有させることがで
きる。また、本発明の効果を損なわない範囲において、
他の細胞賦活剤、抗酸化剤、植物抽出物との併用も可能
である。The external preparation for skin according to the present invention includes oily components, surfactants, humectants, pigments, UV absorbers, antioxidants, in addition to plant extracts of the genus Livistona R. Br. It is possible to add general raw materials for pharmaceuticals and cosmetics such as fragrances and antifungal agents, and physiologically active ingredients. Further, in the range that does not impair the effects of the present invention,
It is also possible to use it in combination with other cell activators, antioxidants and plant extracts.
【0015】[0015]
【実施例】さらに実施例により、本発明の特徴について
詳細に説明する。まず、本発明のビロウ属(Livis
tona R. Br.)植物抽出物の調製例について示
す。EXAMPLES The features of the present invention will be described in more detail with reference to examples. First, the genus Livis of the present invention ( Livis
tona R. Br.) A plant extract is prepared.
【0016】[調製例1]ビロウ抽出物1
ビロウ属(Livistona R. Br.)植物のビ
ロウ(Livistona chinensis (J
acq.) R. Br. var. subglobo
sa (Hassk.) Becc.)の果実1kgに5
0重量%エタノール水溶液を5リットル加え、室温で7
日間浸漬した。抽出液をろ過して回収し、溶媒を除去し
た後、ビロウ抽出物1を得た。[Preparation Example 1] Billow Extract 1 Livistona chinensis (J) of a genus ( Livistona R. Br.) Plant.
acq. ) R. Br. Var. subglobo
sa (Hassk.) Becc.) 5 per 1 kg of fruit
Add 5 liters of 0% by weight ethanol aqueous solution, and add 7% at room temperature.
Soaked for a day. The extract was filtered and recovered, and the solvent was removed to obtain a velvet extract 1.
【0017】[調製例2]ジャワビロウ抽出物
ビロウ属(Livistona R. Br.)植物のジ
ャワビロウ(Livistona rotundifo
lia Mart.)の葉1kgに水を9リットル加
え、90℃にて6時間還流して抽出した。抽出液をろ過
して回収し、溶媒を除去した後、ジャワビロウ抽出物を
得た。[Preparation Example 2] Javanese wax extract Javanese wax ( Livistona rotundifo) of the genus ( Livistona R. Br.) Plant
(1 Lia Mart.) leaves (1 kg) were added with 9 liters of water and refluxed at 90 ° C. for 6 hours for extraction. The extract was filtered and collected, and after removing the solvent, a Javanese wax extract was obtained.
【0018】[調製例3]オガサワラビロウ抽出物
ビロウ属(Livistona R. Br.)植物のオ
ガサワラビロウ(Livistona chinens
is R. Br. var. boninensis
Becc.)の葉と枝の混合物1kgにエタノールを9
リットル加え、室温で7日間浸漬した。抽出液をろ過し
て回収し、溶媒を除去した後、オガサワラビロウ抽出物
を得た。[Preparation Example 3] Ogasawara berow extract [Livistona chinens] of the genus ( Livistona R. Br.) Plant
is R. Br. var. boninensis
Becc. ) Ethanol is added to 1 kg of a mixture of leaves and branches.
1 liter was added and it was immersed at room temperature for 7 days. The extract was filtered and collected, and the solvent was removed to obtain an Ogasawara wax extract.
【0019】[調製例4]ビロウ抽出物2
超臨界抽出装置にビロウ属(Livistona R.
Br.)植物のビロウ(Livistona chin
ensis (Jacq.) R. Br. var. s
ubglobosa (Hassk.) Becc.)の
種子を投入し、二酸化炭素の超臨界流体によって抽出し
た。抽出物を回収し、ビロウ抽出物2を得た。[0019] [Preparation Example 4] Below extract 2 Below genus supercritical extractor (Livistona R.
Br.) Plant Birow (Livistona chin)
ensis (Jacq.) R. Br. var. s
ubgloosa (Hassk.) Becc.) seeds were introduced and extracted with a supercritical fluid of carbon dioxide. The extract was recovered to obtain a velvet extract 2.
【0020】次に、ビロウ抽出物1及びビロウ抽出物2
の真皮線維芽細胞賦活作用を示す。Next, velvet extract 1 and velvet extract 2
Shows the dermal fibroblast activation effect of.
【0021】評価は、以下の手順で行った。正常ヒト真
皮線維芽細胞を1ウェル当たり2.0×104個となる
ように96穴マイクロプレートに播種した。播種培地に
は、市販のクラボウ社製Humedia−KG2を用い
た。24時間培養後、ビロウ抽出物を添加した試験培地
に交換し、さらに24時間培養した。次いで3−(4,5
−ジメチル−2−チアゾリル)−2,5−ジフェニルテト
ラゾリウムブロミド(MTT)を100μg/ml含有
する培地に交換して2時間培養し、テトラゾリウム環の
開環により生じるフォルマザンを2−プロパノールにて
抽出し、マイクロプレートリーダーにて550nmの吸
光度を測定した。同時に濁度として650nmにおける
吸光度を測定し、両測定値の差により細胞賦活作用を評
価した。結果を、ビロウ抽出物無添加の場合の細胞賦活
作用を100とした場合の相対値にて表1に示す。The evaluation was carried out by the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 4 cells per well. Commercially available Humedia-KG2 manufactured by Kurabo Industries was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with a test medium to which a betel extract was added, and the cells were further cultivated for 24 hours. Then 3- (4,5
-Dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 µg / ml and cultured for 2 hours, and formazan generated by ring opening of tetrazolium ring was extracted with 2-propanol. The absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as the turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The results are shown in Table 1 as a relative value when the cell activating effect without addition of the betel extract is 100.
【0022】[0022]
【表1】 [Table 1]
【0023】表1より、ビロウ抽出物1では、ビロウ抽
出物1を0.25〜0.5mg/ml添加した場合に、
無添加の場合と比較して、危険率1%未満で有意な真皮
線維芽細胞賦活作用が認められていた。また、ビロウ抽
出物2でも、ビロウ抽出物2を1.0mg/ml添加し
た場合に、無添加の場合と比較して、危険率1%未満で
有意な真皮線維芽細胞賦活作用が認められていた。これ
らのことから、ビロウ抽出物は、優れた真皮線維芽細胞
賦活作用を有することが明らかとなった。From Table 1, in the case of the velvet extract 1, when 0.25 to 0.5 mg / ml of the velvet extract 1 was added,
Compared with the case of no addition, a significant dermal fibroblast activation effect was recognized at a risk rate of less than 1%. In addition, in velvet extract 2, when velvet extract 2 was added at 1.0 mg / ml, a significant dermal fibroblast activating effect was observed at a risk rate of less than 1% as compared with the case without addition. It was From these, it was revealed that the velvet extract has an excellent dermal fibroblast activation effect.
【0024】次に、ビロウ抽出物の抗酸化作用について
示す。Next, the antioxidant action of the velvet extract will be described.
【0025】評価は、以下の手順で行った。50重量%
エタノール水溶液にて0.1mg/mlに希釈したビロ
ウ抽出物溶液を96穴マイクロプレートに100μl添
加した。次に0.2mMの濃度になるようエタノールに
て調製した1,1−ジフェニル−2−ピクリルヒドラジ
ル(DPPH)溶液を、96穴マイクロプレートに10
0μl添加した。攪拌しながら暗所にて10分間放置し
た後、516nmの吸光度を測定した。ビロウ抽出物無
添加の場合の吸光度を(A)、ビロウ抽出物溶液の吸光
度を(B)としたとき、式(1)の値をラジカル消去率
とした。
式(1) {1−(B)/(A)}×100(%)
ビロウ抽出物1の実験結果を表2に示す。The evaluation was carried out by the following procedure. 50% by weight
100 μl of a velvet extract solution diluted to 0.1 mg / ml with an aqueous ethanol solution was added to a 96-well microplate. Next, a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was placed on a 96-well microplate at 10
0 μl was added. After leaving for 10 minutes in the dark with stirring, the absorbance at 516 nm was measured. Letting (A) be the absorbance without addition of the bevel extract and (B) be the absorbance of the bevel extract solution, the value of the formula (1) was taken as the radical scavenging rate. Table 2 shows the experimental results of the formula (1) {1- (B) / (A)} × 100 (%) velvet extract 1.
【0026】[0026]
【表2】 [Table 2]
【0027】表2より明らかなように、ビロウ抽出物に
は優れた抗酸化作用があることが分かった。As is clear from Table 2, it was found that the velvet extract has an excellent antioxidant effect.
【0028】続いて、本発明に係るビロウ属(Livi
stona R. Br.)植物抽出物を配合した実施例
の処方を示す。Next, the genus Virou ( Livi according to the present invention
(Stona R. Br.) plant extract.
【0029】
[実施例1]化粧水
(1)エタノール 15.0(重量%)
(2)ポリオキシエチレン(40E.O.)硬化ヒマシ油 0.3
(3)香料 0.1
(4)精製水 79.38
(5)クエン酸 0.02
(6)クエン酸ナトリウム 0.1
(7)グリセリン 3.0
(8)ヒドロキシエチルセルロース 0.1
(9)ビロウ抽出物1 1.0
(10)ビロウ抽出物2 1.0
製法:(1)に(2)及び(3)を溶解する。溶解後、
(4)〜(8)を順次添加した後、十分に攪拌し、
(9)と(10)を加え、均一に混合する。[Example 1] Lotion (1) Ethanol 15.0 (wt%) (2) Polyoxyethylene (40 EO) hydrogenated castor oil 0.3 (3) Perfume 0.1 (4) Purification Water 79.38 (5) Citric acid 0.02 (6) Sodium citrate 0.1 (7) Glycerin 3.0 (8) Hydroxyethylcellulose 0.1 (9) Bevel extract 1 1.0 (10) Bevel Extract 2 1.0 Manufacturing method: (2) and (3) are dissolved in (1). After dissolution
After sequentially adding (4) to (8), thoroughly stir,
Add (9) and (10) and mix evenly.
【0030】
[実施例2]乳液
(1)スクワラン 10.0(重量%)
(2)メチルフェニルポリシロキサン 4.0
(3)水素添加パーム核油 0.5
(4)水素添加大豆リン脂質 0.1
(5)モノステアリン酸ポリオキシエチレン
ソルビタン(20E.O.) 1.3
(6)モノステアリン酸ソルビタン 1.0
(7)グリセリン 10.0
(8)パラオキシ安息香酸メチル 0.1
(9)カルボキシビニルポリマー 0.15
(10)精製水 52.83
(11)アルギニン(10重量%水溶液) 20.0
(12)ジャワビロウ抽出物 0.01
(13)ビロウ抽出物2 0.01
製法:(1)〜(6)の油相成分を80℃にて加熱溶解
する。一方(7)〜(10)の水相成分を80℃にて加熱
溶解する。これに前記油相成分を攪拌しながら加え、ホ
モジナイザーにより均一に乳化する。乳化終了後、冷却
を開始し、(11)〜(13)を順次加え、均一に混合す
る。[Example 2] Emulsion (1) Squalane 10.0 (wt%) (2) Methylphenylpolysiloxane 4.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0 1 (5) Polyoxyethylene sorbitan monostearate (20 EO) 1.3 (6) Sorbitan monostearate 1.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9 ) Carboxyvinyl polymer 0.15 (10) Purified water 52.83 (11) Arginine (10% by weight aqueous solution) 20.0 (12) Java wax extract 0.01 (13) Bevel extract 2 0.01 Production method: ( The oil phase components 1) to 6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After the emulsification is completed, cooling is started, and (11) to (13) are sequentially added and mixed uniformly.
【0031】
[実施例3]クリーム
(1)スクワラン 10.0(重量%)
(2)ステアリン酸 2.0
(3)水素添加パーム核油 0.5
(4)水素添加大豆リン脂質 0.1
(5)セタノール 3.6
(6)親油型モノステアリン酸グリセリン 2.0
(7)グリセリン 10.0
(8)パラオキシ安息香酸メチル 0.1
(9)カルボキシビニルポリマー 0.15
(10)精製水 56.35
(11)アルギニン(20重量%水溶液) 15.0
(12)ビロウ抽出物1 0.1
(13)オガサワラビロウ抽出物 0.1
製法:(1)〜(6)の油相成分を80℃にて加熱溶解
する。一方(7)〜(10)の水相成分を80℃にて加熱
溶解する。これに前記油相成分を攪拌しながら加え、ホ
モジナイザーにより均一に乳化する。乳化終了後、冷却
を開始し、50℃にて(11)を加え、40℃にて(12)
と(13)を加え、均一に混合する。Example 3 Cream (1) Squalane 10.0 (wt%) (2) Stearic Acid 2.0 (3) Hydrogenated Palm Kernel Oil 0.5 (4) Hydrogenated Soybean Phospholipid 0.1 (5) Cetanol 3.6 (6) Lipophilic glyceryl monostearate 2.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9) Carboxyvinyl polymer 0.15 (10) Purification Water 56.35 (11) Arginine (20 wt% aqueous solution) 15.0 (12) Bevel extract 1 0.1 (13) Ogasawara berow extract 0.1 Process: Oil phase components of (1) to (6) Is dissolved by heating at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After completion of emulsification, start cooling, add (11) at 50 ° C, and (12) at 40 ° C.
Add (13) and mix evenly.
【0032】
[実施例4]水性ジェル
(1)カルボキシビニルポリマー 0.5(重量%)
(2)精製水 85.7
(3)水酸化ナトリウム(10重量%水溶液) 0.5
(4)エタノール 10.0
(5)パラオキシ安息香酸メチル 0.1
(6)香料 0.1
(7)ポリオキシエチレン(60E.O.)硬化ヒマシ油 0.1
(8)グリセリン 2.0
(9)ジャワビロウ抽出物 0.5
(10)ビロウ抽出物1 0.5
製法:(1)を(2)に加え、均一に攪拌した後、
(3)を加える。均一に攪拌した後,(4)に予め溶解
した(5)を加える。均一に攪拌した後、予め混合して
おいた(6)と(7)を加える。均一に攪拌した後、
(8)〜(10)を順次加え、均一に混合する。[Example 4] Aqueous gel (1) Carboxyvinyl polymer 0.5 (wt%) (2) Purified water 85.7 (3) Sodium hydroxide (10 wt% aqueous solution) 0.5 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Perfume 0.1 (7) Polyoxyethylene (60 EO) hydrogenated castor oil 0.1 (8) Glycerin 2.0 (9) Java wax extraction Product 0.5 (10) Bevel Extract 1 0.5 Manufacturing method: (1) was added to (2), and after stirring uniformly,
Add (3). After stirring uniformly, (5) dissolved in advance in (4) is added. After stirring uniformly, (6) and (7) that have been mixed in advance are added. After stirring evenly,
(8) to (10) are sequentially added and mixed uniformly.
【0033】
[実施例5]美容液
(1)精製水 32.35(重量%)
(2)グリセリン 10.0
(3)ショ糖脂肪酸エステル 1.3
(4)カルボキシビニルポリマー(1重量%水溶液) 17.5
(5)アルギン酸ナトリウム(1重量%水溶液) 15.0
(6)モノラウリン酸ポリグリセリル 1.0
(7)マカデミアナッツ油脂肪酸フィトステリル 3.0
(8)N-ラウロイル-L-グルタミン酸
ジ(フィトステリル−2−オクチルドデシル) 2.0
(9)硬化パーム油 2.0
(10)スクワラン(オリーブ由来) 1.0
(11)ベヘニルアルコール 0.75
(12)ミツロウ 1.0
(13)ホホバ油 1.0
(14)1,3−ブチレングリコール 10.0
(15)L−アルギニン(10重量%水溶液) 2.0
(16)オガサワラビロウ抽出物 0.05
(17)ビロウ抽出物2 0.05
製法:(1)〜(6)の水相成分を混合し、75℃にて
加熱溶解する。一方、(7)〜(14)の油相成分を混合
し、75℃にて加熱溶解する。次いで、上記水相成分に
油相成分を添加して予備乳化を行った後、ホモミキサー
にて均一に乳化する。乳化終了後に冷却を開始し、50
℃にて(15)を加える。さらに40℃まで冷却し、(1
6)と(17)を加え、均一に混合する[Example 5] Beauty liquid (1) Purified water 32.35 (wt%) (2) Glycerin 10.0 (3) Sucrose fatty acid ester 1.3 (4) Carboxyvinyl polymer (1 wt% aqueous solution) ) 17.5 (5) Sodium alginate (1% by weight aqueous solution) 15.0 (6) Polyglyceryl monolaurate 1.0 (7) Macadamia nut oil fatty acid phytosteryl 3.0 (8) N-lauroyl-L-glutamate di (phytosteryl) -2-octyldodecyl) 2.0 (9) hydrogenated palm oil 2.0 (10) squalane (derived from olive) 1.0 (11) behenyl alcohol 0.75 (12) beeswax 1.0 (13) jojoba oil 1. 0 (14) 1,3-butylene glycol 10.0 (15) L-arginine (10% by weight aqueous solution) 2.0 (16) Ogasawara wax extract 0.05 (1 7) Birow extract 2 0.05 Manufacturing method: The aqueous phase components of (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and heated and dissolved at 75 ° C. Next, the oil phase component is added to the above water phase component to carry out preliminary emulsification, and then uniformly emulsified with a homomixer. Start cooling after the emulsification is completed, and
Add (15) at ℃. Further cool to 40 ℃, (1
Add 6) and (17) and mix evenly
【0034】
[実施例6]クレンジング料
(1)スクワラン 80.0
(2)イソステアリン酸ポリオキシエチレングリセリル 15.0
(3)精製水 3.0
(4)ビロウ抽出物1 1.0
(5)ビロウ抽出物2 1.0
製法:(1)と(2)を均一に溶解する。これに、
(3)〜(5)を順次加え、均一に溶解する。[Example 6] Cleansing agent (1) Squalane 80.0 (2) Polyoxyethylene glyceryl isostearate 15.0 (3) Purified water 3.0 (4) Bevel extract 1 1.0 (5) Billow extract 2 1.0 Manufacturing method: (1) and (2) are uniformly dissolved. to this,
(3) to (5) are added in sequence and dissolved uniformly.
【0035】
[実施例7]洗顔フォーム
(1)ステアリン酸 16.0(重量%)
(2)ミリスチン酸 16.0
(3)親油型モノステアリン酸グリセリン 2.0
(4)グリセリン 20.0
(5)水酸化ナトリウム 7.5
(6)ヤシ油脂肪酸アミドプロピルベタイン 1.0
(7)精製水 36.5
(8)ジャワビロウ抽出物 0.5
(9)オガサワラビロウ抽出物 0.5
製法:(1)〜(4)の油相成分を80℃にて加熱溶解
する。一方(5)〜(7)の水相成分を80℃にて加熱
溶解し、油相成分と均一に混合撹拌する。冷却を開始
し、45℃にて(8)と(9)を加え、均一に混合す
る。Example 7 Face Wash Foam (1) Stearic Acid 16.0 (wt%) (2) Myristic Acid 16.0 (3) Lipophilic Monostearic Acid Glycerin 2.0 (4) Glycerin 20.0 (5) Sodium hydroxide 7.5 (6) Coconut oil fatty acid amide propyl betaine 1.0 (7) Purified water 36.5 (8) Java wax extract 0.5 (9) Ogasawara wax extract 0.5 Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and uniformly mixed with the oil phase component and stirred. Start cooling, add (8) and (9) at 45 ° C., and mix evenly.
【0036】
[実施例8]メイクアップベースクリーム
(1)スクワラン 10.0(重量%)
(2)セタノール 2.0
(3)グリセリントリ−2−エチルヘキサン酸エステル 2.5
(4)親油型モノステアリン酸グリセリル 1.0
(5)プロピレングリコール 11.0
(6)ショ糖脂肪酸エステル 1.3
(7)精製水 70.4
(8)酸化チタン 1.0
(9)ベンガラ 0.1
(10)黄酸化鉄 0.4
(11)香料 0.1
(12)ビロウ抽出物1 0.1
(13)ジャワビロウ抽出物 0.1
製法:(1)〜(4)の油相成分を混合し、75℃にて
加熱溶解後する。一方、(5)〜(7)の水相成分を混
合し、75℃にて加熱溶解し、これに(8)〜(10)の
顔料を加え、ホモミキサーにて均一に分散させる。この
水相成分に前記油相成分を加え、ホモミキサーにて乳化
する。乳化終了後に冷却を開始し、40℃にて(11)〜
(13)の成分を加え、均一に混合する。Example 8 Makeup Base Cream (1) Squalane 10.0 (wt%) (2) Cetanol 2.0 (3) Glycerin Tri-2-ethylhexanoate 2.5 (4) Lipophilic Glyceryl monostearate 1.0 (5) Propylene glycol 11.0 (6) Sucrose fatty acid ester 1.3 (7) Purified water 70.4 (8) Titanium oxide 1.0 (9) Red iron oxide 0.1 ( 10) Yellow iron oxide 0.4 (11) Perfume 0.1 (12) Bevel extract 1 0.1 (13) Java beeswax extract 0.1 Production method: Mix the oil phase components of (1) to (4) After heating and melting at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, heated and dissolved at 75 ° C., the pigments (8) to (10) are added thereto, and the components are uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component and emulsified with a homomixer. After the emulsification is completed, cooling is started, and at 40 ° C (11) ~
Add the component (13) and mix evenly.
【0037】
[実施例9]乳液状ファンデーション
(1)メチルポリシロキサン 2.0(重量%)
(2)スクワラン 5.0
(3)ミリスチン酸オクチルドデシル 5.0
(4)セタノール 1.0
(5)ポリオキシエチレン(20E.O.)
ソルビタンモノステアリン酸エステル 1.3
(6)モノステアリン酸ソルビタン 0.7
(7)1,3−ブチレングリコール 8.0
(8)キサンタンガム 0.1
(9)パラオキシ安息香酸メチル 0.1
(10)精製水 58.52
(11)酸化チタン 9.0
(12)タルク 7.4
(13)ベンガラ 0.5
(14)黄酸化鉄 1.1
(15)黒酸化鉄 0.1
(16)香料 0.1
(17)ジャワビロウ抽出物 0.04
(18)オガサワラビロウ抽出物 0.04
製法:(1)〜(6)の油相成分を混合し、75℃にて
加熱溶解する。一方、(7)〜(10)の水相成分を混合
し、75℃にて加熱溶解し、これに(11)〜(15)の顔
料を加え、ホモミキサーにて均一に分散する。油相成分
を加え、乳化を行う。乳化終了後に冷却を開始し、40
℃にて(16)〜(18)の成分を加え、均一に混合する。[Example 9] Emulsion foundation (1) Methyl polysiloxane 2.0 (wt%) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5 ) Polyoxyethylene (20EO) sorbitan monostearate 1.3 (6) sorbitan monostearate 0.7 (7) 1,3-butylene glycol 8.0 (8) xanthan gum 0.1 (9) Methyl paraoxybenzoate 0.1 (10) Purified water 58.52 (11) Titanium oxide 9.0 (12) Talc 7.4 (13) Red iron oxide 0.5 (14) Yellow iron oxide 1.1 (15) Black Iron oxide 0.1 (16) Fragrance 0.1 (17) Java wax extract 0.04 (18) Ogasawara wax extract 0.04 Process: Mix the oil phase components of (1) to (6) at 75 ° C. Melted at To. On the other hand, the aqueous phase components (7) to (10) are mixed, heated and dissolved at 75 ° C., the pigments (11) to (15) are added thereto, and the components are uniformly dispersed with a homomixer. Add the oil phase component and emulsify. Cooling is started after the emulsification is completed, and 40
Add the components (16) to (18) at ℃ and mix evenly.
【0038】
[実施例10]油中水型エモリエントクリーム
(1)流動パラフィン 30.0(重量%)
(2)マイクロクリスタリンワックス 2.0
(3)ワセリン 5.0
(4)ジグリセリンオレイン酸エステル 5.0
(5)塩化ナトリウム 1.3
(6)塩化カリウム 0.1
(7)プロピレングリコール 3.0
(8)1,3−ブチレングリコール 5.0
(9)パラオキシ安息香酸メチル 0.1
(10)オガサワラビロウ抽出物 2.0
(11)ビロウ抽出物1 0.5
(12)精製水 45.9
(13)香料 0.1
製法:(5)と(6)を(12)の一部に溶解して50℃
とし、50℃に加熱した(4)に撹拌しながら徐々に加
える。これを混合した後、70℃にて加熱溶解した
(1)〜(3)に均一に分散する。これに(7)〜(1
1)を(12)の残部に70℃にて加熱溶解したものを撹
拌しながら加え、ホモミキサーにて乳化する。乳化終了
後に冷却を開始し、40℃にて(13)を加え、均一に混
合する。[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglycerin oleate 5.0 (5) Sodium chloride 1.3 (6) Potassium chloride 0.1 (7) Propylene glycol 3.0 (8) 1,3-Butylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 ( 10) Ogasawara Below Extract 2.0 (11) Below Extract 1 0.5 (12) Purified Water 45.9 (13) Perfume 0.1 Manufacturing Method: Part (12) of (5) and (6) Dissolved in 50 ℃
And heated gradually to (4) heated to 50 ° C. with stirring. After mixing this, it melt | dissolves by heating at 70 degreeC and it disperses uniformly in (1)-(3). To this (7) ~ (1
What was heated and dissolved at 70 ° C. was added to the rest of (12) with stirring, and the mixture was emulsified with a homomixer. After the completion of emulsification, cooling is started, and (13) is added at 40 ° C. and mixed uniformly.
【0039】 [実施例11]ヘアートニック (1)エタノール 50.0(重量%) (2)精製水 49.898 (3)オガサワラビロウ抽出物 0.001 (4)ビロウ抽出物2 0.001 (5)香料 0.1 製法:(1)〜(5)の成分を混合,均一化する。[0039] [Example 11] Heart nick (1) Ethanol 50.0 (wt%) (2) Purified water 49.898 (3) Ogasawara wax extract 0.001 (4) Bevel extract 2 0.001 (5) Perfume 0.1 Manufacturing method: Components (1) to (5) are mixed and homogenized.
【0040】本発明の実施例1〜5について使用試験を
行い、シワ、タルミ、肌のハリ、及びクスミの改善効果
を評価した。その際、実施例1において、配合したビロ
ウ抽出物を精製水に代替し、比較例1として同時に使用
試験を行った。A use test was conducted on Examples 1 to 5 of the present invention to evaluate the effects of improving wrinkles, tarmi, skin firmness, and dullness. At that time, in Example 1, the blended betel extract was replaced with purified water, and a use test was simultaneously performed as Comparative Example 1.
【0041】各試料について、シワ、タルミ、肌のハ
リ、及びクスミといった老化症状が顕著に認められる5
0〜60才代の男女パネラー各20名にブラインドにて
1カ月間使用させ、使用前後の皮膚状態の変化を観察し
て評価した。皮膚の老化症状の指標として、シワ、タル
ミ、肌のハリ、及びクスミについて、「改善」,「やや
改善」,「変化なし」の三段階で評価し、表3に各評価
を得たパネラー数にて示した。Aging symptoms such as wrinkles, tarmi, skin firmness, and dullness are noticeable in each sample. 5
Twenty male and female panelists in their 0s to 60s were blindly used for 1 month, and changes in skin condition before and after use were observed and evaluated. As an index of skin aging symptoms, wrinkles, tarmi, skin firmness, and dullness were evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and the number of panelists who obtained each evaluation in Table 3 It showed in.
【0042】[0042]
【表3】 [Table 3]
【0043】表3より、シワ、タルミ、肌のハリ、及び
クスミについて、ビロウ属(Livistona R.
Br.)植物抽出物を含有しない比較例使用群において
は、ほとんど改善が認められなかったが、ビロウ属(L
ivistona R. Br.)植物抽出物を配合した
実施例使用群においては、6割以上のパネラーに明確な
改善が認められた。From Table 3, wrinkles, tarmi, skin firmness, and dullness are listed in the genus Livistona R.
Br.) In the comparative example using a group not containing a plant extract, although little improvement was observed, Below genus (L
Ivistona R. Br.) In the use group of the Example containing the plant extract, a clear improvement was observed in 60% or more of the panelists.
【0044】以上のように、本発明の実施例において
は、従来の比較例よりも、シワ、タルミ、肌のハリ、及
びクスミといった皮膚の老化症状の改善に優れた効果を
有していた。As described above, the examples of the present invention were more effective than the conventional comparative examples in the improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness and dullness.
【0045】[0045]
【発明の効果】以上詳述したように、本発明により、線
維芽細胞賦活作用と抗酸化作用を有し、シワ、タルミ、
肌のハリの低下、及びクスミなどの老化症状の予防や改
善に優れた効果を発揮する皮膚外用剤を得ることが出来
た。INDUSTRIAL APPLICABILITY As described in detail above, according to the present invention, it has a fibroblast activating effect and an antioxidant effect, and has wrinkles, tarmi,
It was possible to obtain a skin external preparation that exhibits excellent effects in reducing skin firmness and preventing and improving aging symptoms such as dullness.
───────────────────────────────────────────────────── フロントページの続き (72)発明者 奥村 由美子 兵庫県神戸市中央区港島中町6丁目13番 地の1 株式会社ノエビア 神戸研究所 内 (72)発明者 山下 由貴 兵庫県神戸市中央区港島中町6丁目13番 地の1 株式会社ノエビア 神戸研究所 内 (72)発明者 京谷 大毅 兵庫県神戸市中央区港島中町6丁目13番 地の1 株式会社ノエビア 神戸研究所 内 (56)参考文献 特開2001−220313(JP,A) 特開2001−10926(JP,A) 特開 平7−17849(JP,A) 特開 平6−271446(JP,A) 特表2002−501501(JP,A) 日本栄養・食糧学会総会講演要旨集, 1999年,Vol.53,p.142 Chemical Abstract s,1996年,Vol.124,No.3, p.432−433,Abs.No.124: 24541 奥田拓男編,天然薬物事典,廣川書 店,1986年 4月15日,第359頁 (58)調査した分野(Int.Cl.7,DB名) A61K 7/00 A61K 35/78 BIOSIS(DIALOG) CA(STN) JICSTファイル(JOIS) MEDLINE(STN)─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Yumiko Okumura 1-13-6 Minatojima Nakamachi, Chuo-ku, Kobe-shi, Hyogo Noevir Co., Ltd. Kobe Research Institute (72) Inventor Yuki Yamashita Nakajima, Minato-jima, Chuo-ku, Kobe-shi, Hyogo Noevir Kobe Research Institute, No. 1 13-6, Machi Nobeer Kobe Research Laboratory, Inc. (72) Inventor, Daiki Kyotani Noevir Kobe Research, No. 1-13-6, Minatojima Nakamachi, Chuo-ku, Kobe, Hyogo Prefecture (56) References JP 2001-220313 (JP, A) JP 2001-10926 (JP, A) JP 7-17849 (JP, A) JP 6-271446 (JP, A) JP 2002-501501 (JP, A) Proceedings of the Annual Meeting of the Japanese Society of Nutrition and Food Science, 1999, Vol. 53, p. 142 Chemical Abstracts, 1996, Vol. 124, No. 3, p. 432-433, Abs. No. 124: 24541 Okuda Takuo, Natural Drug Encyclopedia, Hirokawa Shoten, April 15, 1986, page 359 (58) Fields investigated (Int.Cl. 7 , DB name) A61K 7/00 A61K 35/78 BIOSIS (DIALOG) CA (STN) JISST file (JOIS) MEDLINE (STN)
Claims (2)
Br.)植物抽出物を配合することを特徴とする老化防
止及び改善用皮膚外用剤。1. A genus of genus Livistona R.
Br.) A skin external preparation for preventing and improving aging, which contains a plant extract.
Br.)植物抽出物を配合することを特徴とする真皮線
維芽細胞賦活用皮膚外用剤。 2. The genus Livistona R.
Br.) A dermis fibroblast-stimulating external preparation for skin, which contains a plant extract.
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Non-Patent Citations (3)
Title |
---|
Chemical Abstracts,1996年,Vol.124,No.3,p.432−433,Abs.No.124:24541 |
奥田拓男編,天然薬物事典,廣川書店,1986年 4月15日,第359頁 |
日本栄養・食糧学会総会講演要旨集,1999年,Vol.53,p.142 |
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US20210196620A1 (en) * | 2011-03-28 | 2021-07-01 | Mary Kay Inc. | Topical skin care formulations comprising plant extracts |
US11833240B2 (en) * | 2011-03-28 | 2023-12-05 | Mary Kay Inc. | Topical skin care formulations comprising plant extracts |
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