JP3429297B1 - External preparation for skin - Google Patents

Abstract

Kind Code: A1 The skin has an activating effect on dermal fibroblasts and an antioxidant effect, and exhibits excellent effects for preventing and improving aging symptoms such as wrinkles, tarmi, skin firmness, and dark spots. Provide external preparations. [Solution] Wrinkles, tarumi, decrease in skin firmness,
In order to prevent or improve aging symptoms such as scabs and the like, an external preparation for skin is mixed with a plant extract of the genus Livistona ( Livistona R. Br.) Having an activating action of dermal fibroblasts and an antioxidant action.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin having an excellent effect in preventing or ameliorating skin aging symptoms such as generation of wrinkles and spots and reduction of elasticity of skin, and more specifically, dermal fibroblasts. The genus Virowa ( Livistona R. B.) having a cell activating action and an antioxidant action
r.) It relates to a skin external preparation containing a plant extract.

[0002]

2. Description of the Related Art It is known that skin aging phenomena such as generation of wrinkles and spots and reduction of elasticity of skin occur due to oxidative damage such as external stress such as ultraviolet rays. In addition, dryness and functional deterioration of dermal fibroblasts, and reduction and degeneration of dermal matrix such as collagen and elastin are also important factors in the aging phenomenon. In the field of external skin preparations to date, in order to prevent such skin aging, various physiologically active substances such as antioxidants, cell activating agents, collagen production promoters, and elastase activity inhibitors have been searched and studied for compounding. Came. For example, as an antioxidant, an extract of a plant belonging to the genus Heterotheca (Asteraceae) (JP-A-11-180886)
Are known, and examples of the dermal fibroblast activator include loquat extract (Japanese Patent Publication No. 5-17206) and a degradation product of collagen or gelatin with collagenase (JP-A-2000-30).
9521) and the like are known. Further, as a collagen production promoter, peach seed extract (JP-A-11-3
35235), an extract from a tree bud of a beech plant of the family Beech (Japanese Unexamined Patent Publication No. 10-203952), and the like are known. As an elastase activity inhibitor, a combination of a plant extract and glutathione (JP-A-11-263720), a solvent extract of tea tree (JP-A-11-246388) and the like are known.

However, among the physiologically active substances that have already been reported, the action and effect are insufficient, and therefore, when incorporated into the base of the skin external preparation, a considerably high concentration is required to obtain an effective effect. However, the present situation is that there are few that can satisfy all of the effects and stability, such as imparting an unfavorable color or odor to the preparation.

[0004]

Therefore, in the present invention, there is provided a skin external preparation which has a dermal fibroblast activating action and an antioxidant action and is excellent in the prevention and amelioration effect of skin aging symptoms. It was intended.

[0005]

SUMMARY OF THE INVENTION In view of the above-mentioned circumstances, the present inventors have investigated a wide variety of substances for the prevention and improvement of skin aging symptoms based on the dermal fibroblast activation and antioxidant effects. Study was carried out. As a result, they have found an excellent dermal fibroblast activating action and antioxidant action in a plant extract of the genus Livistona R. Br. And have completed the present invention.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION
R. Br.) Plant is a plant of the family Palmidae widely distributed in the subtropical region. In the suburbs of Japan, it is distributed from China and northern Taiwan through the Nansei Islands to Kyushu and the southern part of Shikoku. It is an evergreen tree that grows near the coast and has fan-shaped leaves. Examples of plants belonging to the genus ( Livistona R. Br.) Include:
sis (Jacq.) R. Br. var. subg
lobosa (Hassk.) Becc.), Toubirou (Livistona chinensis R.
Br. Var. chinensis ), Javabill ( Livistona rotundifolia)
Mart.), Australia Below (Livist o
na australis), Ogasawarabiro (Li
vistona chinensis R. Br. va
r. Boninensis Becc. ) Is known.

[0007] These Below genera (Livistona
When using R. Br.) Plants, it is common to use extracts. For extraction, the genus Liviston
a R. Br.) plant trunks, branches, fruits, leaves, flowers, seeds,
Although any part such as bark, sap, root, bud and the like may be used, it is preferable to use leaves or fruits for easy use. When extracting, it may be used as it is, but in consideration of extraction efficiency, it is preferable to perform extraction after performing processes such as shredding, drying and crushing. The extraction can also be performed by immersing in an extraction solvent or by an extraction method using a supercritical fluid or subcritical fluid. In order to improve extraction efficiency, stirring or homogenization in an extraction solvent may be performed. Extraction temperature is 5 ℃
It is appropriate to adjust the temperature to a temperature not higher than the boiling point of the extraction solvent. The extraction time varies depending on the type of extraction solvent and the extraction temperature, but it is suitable to be about 1 hour to 14 days.

As the extraction solvent, in addition to water, methanol,
Lower alcohols such as ethanol, propanol and isopropanol, polyhydric alcohols such as 1,3-butylene glycol, propylene glycol, dipropylene glycol and glycerin, ethers such as ethyl ether and propyl ether, and esters such as ethyl acetate and butyl acetate. It is possible to use polar organic solvents such as acetone, ketones such as ethyl methyl ketone, etc., and one kind or two or more kinds are selected from these and used. Alternatively, physiological saline, phosphate buffer, phosphate buffered saline, etc. may be used. Further, one or more supercritical fluids or subcritical fluids such as water, carbon dioxide, ethylene, propylene, ethanol, methanol and ammonia may be used.

The genus Birowa ( Livistona R. B)
r.) The extract of the plant with the above solvent can be used as it is, but the concentrated and dried product is redissolved in water or a polar solvent, or decolorized within a range not impairing these physiological actions, Purification processing such as deodorization and desalination,
You may use it, after performing a fractionation process by column chromatography etc. The genus Billow ( Livistona R.
Br.) The above-mentioned extracts of plants, their processed products and fractions,
It is also possible to freeze-dry after each treatment and fractionation and dissolve in a solvent before use. It can also be used by encapsulating it in vesicles such as liposomes or in microcapsules.

In the present invention, the genus Livis ( Livis
Tona R. Br.) plant extract, when added to a skin external preparation, exerts an excellent effect in preventing and improving aging symptoms such as wrinkles, tarmi, skin firmness and dullness.

The external preparation for skin having a dermal fibroblast activating action and an antioxidant action according to the present invention is a genus of the genus Virou ( Liv.
stona R. Br.) plant extract as an active ingredient.

The genus Livisto in the present invention
na R. Br.) The amount of the plant extract can be adjusted according to the type and purpose of the skin external preparation, but a suitable amount is 0.0001 based on the total amount of the skin external preparation.
~ 10.0% by weight.

The external preparation for skin according to the present invention is a lotion,
It can be provided in various dosage forms such as emulsion, gel, cream, ointment, powder and granules.

The external preparation for skin according to the present invention includes oily components, surfactants, humectants, pigments, UV absorbers, antioxidants, in addition to plant extracts of the genus Livistona R. Br. It is possible to add general raw materials for pharmaceuticals and cosmetics such as fragrances and antifungal agents, and physiologically active ingredients. Further, in the range that does not impair the effects of the present invention,
It is also possible to use it in combination with other cell activators, antioxidants and plant extracts.

[0015]

EXAMPLES The features of the present invention will be described in more detail with reference to examples. First, the genus Livis of the present invention ( Livis
tona R. Br.) A plant extract is prepared.

[Preparation Example 1] Billow Extract 1 Livistona chinensis (J) of a genus ( Livistona R. Br.) Plant.
acq. ) R. Br. Var. subglobo
sa (Hassk.) Becc.) 5 per 1 kg of fruit
Add 5 liters of 0% by weight ethanol aqueous solution, and add 7% at room temperature.
Soaked for a day. The extract was filtered and recovered, and the solvent was removed to obtain a velvet extract 1.

[Preparation Example 2] Javanese wax extract Javanese wax ( Livistona rotundifo) of the genus ( Livistona R. Br.) Plant
(1 Lia Mart.) leaves (1 kg) were added with 9 liters of water and refluxed at 90 ° C. for 6 hours for extraction. The extract was filtered and collected, and after removing the solvent, a Javanese wax extract was obtained.

[Preparation Example 3] Ogasawara berow extract [Livistona chinens] of the genus ( Livistona R. Br.) Plant
is R. Br. var. boninensis
Becc. ) Ethanol is added to 1 kg of a mixture of leaves and branches.
1 liter was added and it was immersed at room temperature for 7 days. The extract was filtered and collected, and the solvent was removed to obtain an Ogasawara wax extract.

[0019] [Preparation Example 4] Below extract 2 Below genus supercritical extractor (Livistona R.
Br.) Plant Birow (Livistona chin)
ensis (Jacq.) R. Br. var. s
ubgloosa (Hassk.) Becc.) seeds were introduced and extracted with a supercritical fluid of carbon dioxide. The extract was recovered to obtain a velvet extract 2.

Next, velvet extract 1 and velvet extract 2
Shows the dermal fibroblast activation effect of.

The evaluation was carried out by the following procedure. Normal human dermal fibroblasts were seeded in a 96-well microplate at 2.0 × 10 ⁴ cells per well. Commercially available Humedia-KG2 manufactured by Kurabo Industries was used as the seeding medium. After culturing for 24 hours, the culture medium was replaced with a test medium to which a betel extract was added, and the cells were further cultivated for 24 hours. Then 3- (4,5
-Dimethyl-2-thiazolyl) -2,5-diphenyltetrazolium bromide (MTT) was replaced with a medium containing 100 µg / ml and cultured for 2 hours, and formazan generated by ring opening of tetrazolium ring was extracted with 2-propanol. The absorbance at 550 nm was measured with a microplate reader. At the same time, the absorbance at 650 nm was measured as the turbidity, and the cell activation effect was evaluated by the difference between the two measured values. The results are shown in Table 1 as a relative value when the cell activating effect without addition of the betel extract is 100.

[0022]

[Table 1]

From Table 1, in the case of the velvet extract 1, when 0.25 to 0.5 mg / ml of the velvet extract 1 was added,
Compared with the case of no addition, a significant dermal fibroblast activation effect was recognized at a risk rate of less than 1%. In addition, in velvet extract 2, when velvet extract 2 was added at 1.0 mg / ml, a significant dermal fibroblast activating effect was observed at a risk rate of less than 1% as compared with the case without addition. It was From these, it was revealed that the velvet extract has an excellent dermal fibroblast activation effect.

Next, the antioxidant action of the velvet extract will be described.

The evaluation was carried out by the following procedure. 50% by weight
100 μl of a velvet extract solution diluted to 0.1 mg / ml with an aqueous ethanol solution was added to a 96-well microplate. Next, a 1,1-diphenyl-2-picrylhydrazyl (DPPH) solution prepared with ethanol to a concentration of 0.2 mM was placed on a 96-well microplate at 10
0 μl was added. After leaving for 10 minutes in the dark with stirring, the absorbance at 516 nm was measured. Letting (A) be the absorbance without addition of the bevel extract and (B) be the absorbance of the bevel extract solution, the value of the formula (1) was taken as the radical scavenging rate. Table 2 shows the experimental results of the formula (1) {1- (B) / (A)} × 100 (%) velvet extract 1.

[0026]

[Table 2]

As is clear from Table 2, it was found that the velvet extract has an excellent antioxidant effect.

Next, the genus Virou ( Livi according to the present invention
(Stona R. Br.) plant extract.

[Example 1] Lotion (1) Ethanol 15.0 (wt%) (2) Polyoxyethylene (40 EO) hydrogenated castor oil 0.3 (3) Perfume 0.1 (4) Purification Water 79.38 (5) Citric acid 0.02 (6) Sodium citrate 0.1 (7) Glycerin 3.0 (8) Hydroxyethylcellulose 0.1 (9) Bevel extract 1 1.0 (10) Bevel Extract 2 1.0 Manufacturing method: (2) and (3) are dissolved in (1). After dissolution
After sequentially adding (4) to (8), thoroughly stir,
Add (9) and (10) and mix evenly.

[Example 2] Emulsion (1) Squalane 10.0 (wt%) (2) Methylphenylpolysiloxane 4.0 (3) Hydrogenated palm kernel oil 0.5 (4) Hydrogenated soybean phospholipid 0 1 (5) Polyoxyethylene sorbitan monostearate (20 EO) 1.3 (6) Sorbitan monostearate 1.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9 ) Carboxyvinyl polymer 0.15 (10) Purified water 52.83 (11) Arginine (10% by weight aqueous solution) 20.0 (12) Java wax extract 0.01 (13) Bevel extract 2 0.01 Production method: ( The oil phase components 1) to 6) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After the emulsification is completed, cooling is started, and (11) to (13) are sequentially added and mixed uniformly.

Example 3 Cream (1) Squalane 10.0 (wt%) (2) Stearic Acid 2.0 (3) Hydrogenated Palm Kernel Oil 0.5 (4) Hydrogenated Soybean Phospholipid 0.1 (5) Cetanol 3.6 (6) Lipophilic glyceryl monostearate 2.0 (7) Glycerin 10.0 (8) Methyl paraoxybenzoate 0.1 (9) Carboxyvinyl polymer 0.15 (10) Purification Water 56.35 (11) Arginine (20 wt% aqueous solution) 15.0 (12) Bevel extract 1 0.1 (13) Ogasawara berow extract 0.1 Process: Oil phase components of (1) to (6) Is dissolved by heating at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added to this with stirring, and the mixture is uniformly emulsified with a homogenizer. After completion of emulsification, start cooling, add (11) at 50 ° C, and (12) at 40 ° C.
Add (13) and mix evenly.

[Example 4] Aqueous gel (1) Carboxyvinyl polymer 0.5 (wt%) (2) Purified water 85.7 (3) Sodium hydroxide (10 wt% aqueous solution) 0.5 (4) Ethanol 10.0 (5) Methyl paraoxybenzoate 0.1 (6) Perfume 0.1 (7) Polyoxyethylene (60 EO) hydrogenated castor oil 0.1 (8) Glycerin 2.0 (9) Java wax extraction Product 0.5 (10) Bevel Extract 1 0.5 Manufacturing method: (1) was added to (2), and after stirring uniformly,
Add (3). After stirring uniformly, (5) dissolved in advance in (4) is added. After stirring uniformly, (6) and (7) that have been mixed in advance are added. After stirring evenly,
(8) to (10) are sequentially added and mixed uniformly.

[Example 5] Beauty liquid (1) Purified water 32.35 (wt%) (2) Glycerin 10.0 (3) Sucrose fatty acid ester 1.3 (4) Carboxyvinyl polymer (1 wt% aqueous solution) ) 17.5 (5) Sodium alginate (1% by weight aqueous solution) 15.0 (6) Polyglyceryl monolaurate 1.0 (7) Macadamia nut oil fatty acid phytosteryl 3.0 (8) N-lauroyl-L-glutamate di (phytosteryl) -2-octyldodecyl) 2.0 (9) hydrogenated palm oil 2.0 (10) squalane (derived from olive) 1.0 (11) behenyl alcohol 0.75 (12) beeswax 1.0 (13) jojoba oil 1. 0 (14) 1,3-butylene glycol 10.0 (15) L-arginine (10% by weight aqueous solution) 2.0 (16) Ogasawara wax extract 0.05 (1 7) Birow extract 2 0.05 Manufacturing method: The aqueous phase components of (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and heated and dissolved at 75 ° C. Next, the oil phase component is added to the above water phase component to carry out preliminary emulsification, and then uniformly emulsified with a homomixer. Start cooling after the emulsification is completed, and
Add (15) at ℃. Further cool to 40 ℃, (1
Add 6) and (17) and mix evenly

[Example 6] Cleansing agent (1) Squalane 80.0 (2) Polyoxyethylene glyceryl isostearate 15.0 (3) Purified water 3.0 (4) Bevel extract 1 1.0 (5) Billow extract 2 1.0 Manufacturing method: (1) and (2) are uniformly dissolved. to this,
(3) to (5) are added in sequence and dissolved uniformly.

Example 7 Face Wash Foam (1) Stearic Acid 16.0 (wt%) (2) Myristic Acid 16.0 (3) Lipophilic Monostearic Acid Glycerin 2.0 (4) Glycerin 20.0 (5) Sodium hydroxide 7.5 (6) Coconut oil fatty acid amide propyl betaine 1.0 (7) Purified water 36.5 (8) Java wax extract 0.5 (9) Ogasawara wax extract 0.5 Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and uniformly mixed with the oil phase component and stirred. Start cooling, add (8) and (9) at 45 ° C., and mix evenly.

Example 8 Makeup Base Cream (1) Squalane 10.0 (wt%) (2) Cetanol 2.0 (3) Glycerin Tri-2-ethylhexanoate 2.5 (4) Lipophilic Glyceryl monostearate 1.0 (5) Propylene glycol 11.0 (6) Sucrose fatty acid ester 1.3 (7) Purified water 70.4 (8) Titanium oxide 1.0 (9) Red iron oxide 0.1 ( 10) Yellow iron oxide 0.4 (11) Perfume 0.1 (12) Bevel extract 1 0.1 (13) Java beeswax extract 0.1 Production method: Mix the oil phase components of (1) to (4) After heating and melting at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed, heated and dissolved at 75 ° C., the pigments (8) to (10) are added thereto, and the components are uniformly dispersed by a homomixer. The oil phase component is added to the aqueous phase component and emulsified with a homomixer. After the emulsification is completed, cooling is started, and at 40 ° C (11) ~
Add the component (13) and mix evenly.

[Example 9] Emulsion foundation (1) Methyl polysiloxane 2.0 (wt%) (2) Squalane 5.0 (3) Octyldodecyl myristate 5.0 (4) Cetanol 1.0 (5 ) Polyoxyethylene (20EO) sorbitan monostearate 1.3 (6) sorbitan monostearate 0.7 (7) 1,3-butylene glycol 8.0 (8) xanthan gum 0.1 (9) Methyl paraoxybenzoate 0.1 (10) Purified water 58.52 (11) Titanium oxide 9.0 (12) Talc 7.4 (13) Red iron oxide 0.5 (14) Yellow iron oxide 1.1 (15) Black Iron oxide 0.1 (16) Fragrance 0.1 (17) Java wax extract 0.04 (18) Ogasawara wax extract 0.04 Process: Mix the oil phase components of (1) to (6) at 75 ° C. Melted at To. On the other hand, the aqueous phase components (7) to (10) are mixed, heated and dissolved at 75 ° C., the pigments (11) to (15) are added thereto, and the components are uniformly dispersed with a homomixer. Add the oil phase component and emulsify. Cooling is started after the emulsification is completed, and 40
Add the components (16) to (18) at ℃ and mix evenly.

[Example 10] Water-in-oil emollient cream (1) Liquid paraffin 30.0 (% by weight) (2) Microcrystalline wax 2.0 (3) Vaseline 5.0 (4) Diglycerin oleate 5.0 (5) Sodium chloride 1.3 (6) Potassium chloride 0.1 (7) Propylene glycol 3.0 (8) 1,3-Butylene glycol 5.0 (9) Methyl paraoxybenzoate 0.1 ( 10) Ogasawara Below Extract 2.0 (11) Below Extract 1 0.5 (12) Purified Water 45.9 (13) Perfume 0.1 Manufacturing Method: Part (12) of (5) and (6) Dissolved in 50 ℃
And heated gradually to (4) heated to 50 ° C. with stirring. After mixing this, it melt | dissolves by heating at 70 degreeC and it disperses uniformly in (1)-(3). To this (7) ~ (1
What was heated and dissolved at 70 ° C. was added to the rest of (12) with stirring, and the mixture was emulsified with a homomixer. After the completion of emulsification, cooling is started, and (13) is added at 40 ° C. and mixed uniformly.

[0039]   [Example 11] Heart nick (1) Ethanol 50.0 (wt%) (2) Purified water 49.898 (3) Ogasawara wax extract 0.001 (4) Bevel extract 2 0.001 (5) Perfume 0.1 Manufacturing method: Components (1) to (5) are mixed and homogenized.

A use test was conducted on Examples 1 to 5 of the present invention to evaluate the effects of improving wrinkles, tarmi, skin firmness, and dullness. At that time, in Example 1, the blended betel extract was replaced with purified water, and a use test was simultaneously performed as Comparative Example 1.

Aging symptoms such as wrinkles, tarmi, skin firmness, and dullness are noticeable in each sample. 5
Twenty male and female panelists in their 0s to 60s were blindly used for 1 month, and changes in skin condition before and after use were observed and evaluated. As an index of skin aging symptoms, wrinkles, tarmi, skin firmness, and dullness were evaluated in three stages of “improvement”, “slight improvement”, and “no change”, and the number of panelists who obtained each evaluation in Table 3 It showed in.

[0042]

[Table 3]

From Table 3, wrinkles, tarmi, skin firmness, and dullness are listed in the genus Livistona R.
Br.) In the comparative example using a group not containing a plant extract, although little improvement was observed, Below genus (L
Ivistona R. Br.) In the use group of the Example containing the plant extract, a clear improvement was observed in 60% or more of the panelists.

As described above, the examples of the present invention were more effective than the conventional comparative examples in the improvement of skin aging symptoms such as wrinkles, tarmi, skin firmness and dullness.

[0045]

INDUSTRIAL APPLICABILITY As described in detail above, according to the present invention, it has a fibroblast activating effect and an antioxidant effect, and has wrinkles, tarmi,
It was possible to obtain a skin external preparation that exhibits excellent effects in reducing skin firmness and preventing and improving aging symptoms such as dullness.

─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Yumiko Okumura 1-13-6 Minatojima Nakamachi, Chuo-ku, Kobe-shi, Hyogo Noevir Co., Ltd. Kobe Research Institute (72) Inventor Yuki Yamashita Nakajima, Minato-jima, Chuo-ku, Kobe-shi, Hyogo Noevir Kobe Research Institute, No. 1 13-6, Machi Nobeer Kobe Research Laboratory, Inc. (72) Inventor, Daiki Kyotani Noevir Kobe Research, No. 1-13-6, Minatojima Nakamachi, Chuo-ku, Kobe, Hyogo Prefecture (56) References JP 2001-220313 (JP, A) JP 2001-10926 (JP, A) JP 7-17849 (JP, A) JP 6-271446 (JP, A) JP 2002-501501 (JP, A) Proceedings of the Annual Meeting of the Japanese Society of Nutrition and Food Science, 1999, Vol. 53, p. 142 Chemical Abstracts, 1996, Vol. 124, No. 3, p. 432-433, Abs. No. 124: 24541 Okuda Takuo, Natural Drug Encyclopedia, Hirokawa Shoten, April 15, 1986, page 359 (58) Fields investigated (Int.Cl. ⁷ , DB name) A61K 7/00 A61K 35/78 BIOSIS (DIALOG) CA (STN) JISST file (JOIS) MEDLINE (STN)

Claims (2)

(57) [Claims] 1. A genus of genus Livistona R.
Br.) A skin external preparation for preventing and improving aging, which contains a plant extract. 2. The genus Livistona R.
Br.) A dermis fibroblast-stimulating external preparation for skin, which contains a plant extract.