JP2002534986A5 - - Google Patents

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Publication number
JP2002534986A5
JP2002534986A5 JP2000594952A JP2000594952A JP2002534986A5 JP 2002534986 A5 JP2002534986 A5 JP 2002534986A5 JP 2000594952 A JP2000594952 A JP 2000594952A JP 2000594952 A JP2000594952 A JP 2000594952A JP 2002534986 A5 JP2002534986 A5 JP 2002534986A5
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JP
Japan
Prior art keywords
primer
extended
sequence
target
oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2000594952A
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English (en)
Japanese (ja)
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JP2002534986A (ja
JP4657454B2 (ja
Filing date
Publication date
Priority claimed from US09/233,413 external-priority patent/US6200757B1/en
Application filed filed Critical
Publication of JP2002534986A publication Critical patent/JP2002534986A/ja
Publication of JP2002534986A5 publication Critical patent/JP2002534986A5/ja
Application granted granted Critical
Publication of JP4657454B2 publication Critical patent/JP4657454B2/ja
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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JP2000594952A 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法 Expired - Fee Related JP4657454B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/233,413 US6200757B1 (en) 1999-01-19 1999-01-19 Method for controlling the extension of an oligonucleotide
US09/233,413 1999-01-19
PCT/US1999/029253 WO2000043544A1 (en) 1999-01-19 1999-12-10 Method for controlling the extension of an oligonucleotide

Publications (3)

Publication Number Publication Date
JP2002534986A JP2002534986A (ja) 2002-10-22
JP2002534986A5 true JP2002534986A5 (https=) 2007-01-25
JP4657454B2 JP4657454B2 (ja) 2011-03-23

Family

ID=22877144

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2000594952A Expired - Fee Related JP4657454B2 (ja) 1999-01-19 1999-12-10 オリゴヌクレオチドの伸長の制御方法

Country Status (8)

Country Link
US (1) US6200757B1 (https=)
EP (1) EP1147230B1 (https=)
JP (1) JP4657454B2 (https=)
AT (1) ATE322557T1 (https=)
CA (1) CA2358992C (https=)
DE (1) DE69930765T2 (https=)
HK (1) HK1042324B (https=)
WO (1) WO2000043544A1 (https=)

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US6274353B1 (en) 1999-09-22 2001-08-14 Genecopoeia, Inc. Method and compositions for improved polynucleotide synthesis
US7033763B2 (en) * 2000-02-23 2006-04-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP)
AU2001241621B2 (en) * 2000-02-23 2005-08-25 City Of Hope Pyrophosphorolysis activated polymerization (PAP): application to allele-specific amplification and nucleic acid sequence determination
EP1275735A1 (en) * 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US6667165B2 (en) 2001-11-13 2003-12-23 Eppendorf Ag Method and compositions for reversible inhibition of thermostable polymerases
US7211382B2 (en) * 2002-04-09 2007-05-01 Orchid Cellmark Inc. Primer extension using modified nucleotides
WO2004090153A2 (en) * 2003-04-01 2004-10-21 Eragen Biosciences,Inc. Polymerase inhibitor and method of using same
US8293472B2 (en) * 2005-06-07 2012-10-23 Luminex Corporation Methods for detection and typing of nucleic acids
US20070048758A1 (en) * 2005-06-09 2007-03-01 Epoch Biosciences, Inc. Improved primer-based amplification methods
AU2006287548A1 (en) * 2005-09-06 2007-03-15 Gen-Probe Incorporated Methods, compositions and kits for isothermal amplification of nucleic acids
ES2357746T3 (es) * 2006-03-28 2011-04-29 Commonwealth Scientific And Industrial Research Organisation Amplificación de fragmentos de adn.
DE102008005667A1 (de) 2008-01-19 2009-07-30 Olfert Landt Basenmodifizierte Primeroligomere für die Multiplex-RT-PCR
ES2527653T3 (es) * 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
CA2777446A1 (en) * 2009-10-13 2011-04-21 Syntezza Molecular Detection Israel Ltd. Methods and compositions for amplifying target sequences from nucleic acid samples
US9238832B2 (en) 2009-12-11 2016-01-19 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids
US8614071B2 (en) 2009-12-11 2013-12-24 Roche Molecular Systems, Inc. Preferential amplification of mRNA over DNA using chemically modified primers
DK3109326T3 (da) 2010-06-21 2022-02-14 Life Technologies Corp Sammensætning og fremgangsmåde til nukleinsyresyntese og amplifikation under anvendelse af rt
EP2582850B1 (en) 2010-06-21 2016-11-09 Life Technologies Corporation Compositions, kits, and methods for synthesis and/or detection of nucleic acids
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
EP4026912A1 (en) 2012-05-10 2022-07-13 The General Hospital Corporation Methods for determining a nucleotide sequence
JP7016215B2 (ja) 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
US9909169B2 (en) 2014-12-17 2018-03-06 Roche Molecular Systems, Inc. Allele-specific amplification of nucleic acids using blocking oligonucleotides for wild type suppression
WO2018053362A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation
CA3037190A1 (en) 2016-09-15 2018-03-22 ArcherDX, Inc. Methods of nucleic acid sample preparation for analysis of cell-free dna
WO2019231568A1 (en) * 2018-05-31 2019-12-05 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
US11180794B2 (en) 2018-05-31 2021-11-23 Omniome, Inc. Methods and compositions for capping nucleic acids
WO2025007089A1 (en) 2023-06-30 2025-01-02 Life Technologies Corporation Compositions, methods and kits for nucleic acids synthesis and amplification

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US5508178A (en) 1989-01-19 1996-04-16 Rose; Samuel Nucleic acid amplification using single primer
ATE142272T1 (de) * 1989-01-19 1996-09-15 Behringwerke Ag Nukleinsäure-amplifikation unter verwendung eines einzelprimers
US5043272A (en) 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
DE69131891T2 (de) 1990-02-16 2000-06-15 F. Hoffmann-La Roche Ag, Basel Verbesserungen in der spezifität und zweckmässigkeit der polymerase-kettenreaktion
US5348853A (en) 1991-12-16 1994-09-20 Biotronics Corporation Method for reducing non-specific priming in DNA amplification
US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5565339A (en) 1992-10-08 1996-10-15 Hoffmann-La Roche Inc. Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents
US5599660A (en) 1993-01-19 1997-02-04 Pharmacia Biotech Inc. Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents
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US5705366A (en) 1994-09-15 1998-01-06 Johnson & Johnson Clinical Diagnostics, Inc. Coamplification of target nucleic acids using volume exclusion agent in reaction composition, test kit and test device useful therefor
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CA2217143A1 (en) * 1996-02-01 1997-08-07 Behringwerke Aktiengesellschaft Positive controls for use in polynucleotide amplification
JP3545158B2 (ja) * 1996-03-14 2004-07-21 シスメックス株式会社 遺伝子検出法
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification
ATE280177T1 (de) * 1997-03-20 2004-11-15 Hoffmann La Roche Modifizierte primer

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