JP2002512252A5 - - Google Patents
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- Publication number
- JP2002512252A5 JP2002512252A5 JP2000544678A JP2000544678A JP2002512252A5 JP 2002512252 A5 JP2002512252 A5 JP 2002512252A5 JP 2000544678 A JP2000544678 A JP 2000544678A JP 2000544678 A JP2000544678 A JP 2000544678A JP 2002512252 A5 JP2002512252 A5 JP 2002512252A5
- Authority
- JP
- Japan
- Prior art keywords
- solution
- silica
- matrix
- nucleic acid
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 83
- 239000011159 matrix material Substances 0.000 description 40
- 239000000377 silicon dioxide Substances 0.000 description 37
- 238000000034 method Methods 0.000 description 36
- 230000003196 chaotropic effect Effects 0.000 description 16
- 239000002158 endotoxin Substances 0.000 description 16
- 108020004707 nucleic acids Proteins 0.000 description 16
- 150000007523 nucleic acids Chemical class 0.000 description 16
- 102000039446 nucleic acids Human genes 0.000 description 16
- 239000003153 chemical reaction reagent Substances 0.000 description 15
- 239000006167 equilibration buffer Substances 0.000 description 8
- 239000013612 plasmid Substances 0.000 description 6
- 230000027455 binding Effects 0.000 description 5
- 239000011230 binding agent Substances 0.000 description 5
- 239000000741 silica gel Substances 0.000 description 5
- 229910002027 silica gel Inorganic materials 0.000 description 5
- 239000005909 Kieselgur Substances 0.000 description 4
- 239000012149 elution buffer Substances 0.000 description 4
- 239000005337 ground glass Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000001914 filtration Methods 0.000 description 3
- 239000006249 magnetic particle Substances 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 150000003839 salts Chemical group 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- 230000004568 DNA-binding Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000002738 chelating agent Substances 0.000 description 1
- 239000005289 controlled pore glass Substances 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical group [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 239000005373 porous glass Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/064,449 US6194562B1 (en) | 1998-04-22 | 1998-04-22 | Endotoxin reduction in nucleic acid purification |
| US09/064,449 | 1998-04-22 | ||
| PCT/US1999/008491 WO1999054340A1 (en) | 1998-04-22 | 1999-04-22 | Endotoxin reduction in nucleic acid purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2002512252A JP2002512252A (ja) | 2002-04-23 |
| JP2002512252A5 true JP2002512252A5 (enExample) | 2006-01-05 |
Family
ID=22056061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000544678A Pending JP2002512252A (ja) | 1998-04-22 | 1999-04-22 | 核酸の精製における内毒素の低減 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6194562B1 (enExample) |
| EP (1) | EP1071695A4 (enExample) |
| JP (1) | JP2002512252A (enExample) |
| AU (1) | AU740145B2 (enExample) |
| CA (1) | CA2329067C (enExample) |
| WO (1) | WO1999054340A1 (enExample) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59505786D1 (de) * | 1994-02-07 | 1999-06-02 | Qiagen Gmbh | Verfahren zur abreicherung oder entfernung von endotoxinen |
| GB9425138D0 (en) | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| US6914137B2 (en) * | 1997-12-06 | 2005-07-05 | Dna Research Innovations Limited | Isolation of nucleic acids |
| ATE386044T1 (de) * | 1997-12-06 | 2008-03-15 | Invitrogen Corp | Isolierung von nukleinsäuren |
| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| WO2000044928A2 (en) * | 1999-01-27 | 2000-08-03 | Halaka Folim G | Materials and methods for the purification of polyelectrolytes |
| DE19903507A1 (de) * | 1999-01-29 | 2000-08-10 | Roche Diagnostics Gmbh | Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung |
| WO2002000342A2 (en) * | 2000-06-23 | 2002-01-03 | Irm, Llc | Method and apparatus for performing an assay |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| WO2002044414A2 (en) * | 2000-11-28 | 2002-06-06 | Promega Corporation | Purification of dna sequencing reactions using silica magnetic particles |
| KR100463415B1 (ko) * | 2001-12-12 | 2004-12-23 | 씨제이 주식회사 | 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법 |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| US7183104B1 (en) | 2002-08-23 | 2007-02-27 | Duane Morris Llp | Separator and particle detection system |
| WO2005012487A2 (en) * | 2003-08-01 | 2005-02-10 | Invitrogen Corporation | Compositions and methods for preparing short rna molecules and other nucleic acids |
| EP1709197A4 (en) * | 2003-12-30 | 2007-07-04 | Sigma Aldrich Co | RAPID PREPARATION OF NUCLEIC ACIDS BY ENZYMATIC DEPENDENCE |
| US7531308B2 (en) * | 2004-04-23 | 2009-05-12 | Sigma-Aldrich Co. | Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography |
| JP2008510456A (ja) | 2004-07-30 | 2008-04-10 | アジェンコート バイオサイエンス コーポレーション | 多官能基コート化固相担体を用いる核酸の単離方法 |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| JP2009500019A (ja) * | 2005-07-01 | 2009-01-08 | プロメガ・コーポレーション | 生体分子の精製のための浮遊性粒子のネットワーク、及び生体分子の精製のための浮遊性粒子又は浮遊性粒子のネットワークの使用 |
| DE102005059217B4 (de) * | 2005-12-07 | 2011-03-17 | Aj Innuscreen Gmbh | Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren |
| EP1963526A4 (en) | 2005-12-09 | 2009-11-18 | Promega Corp | PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX |
| CN101091797B (zh) * | 2006-06-23 | 2012-08-01 | 上海海规生物科技有限公司 | 去除初纯质粒或蛋白质溶液中内毒素的方法及试剂盒 |
| US8163535B2 (en) * | 2006-06-26 | 2012-04-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| US7608399B2 (en) * | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| CN103255130B (zh) * | 2008-04-30 | 2015-12-23 | 斯特莱科生物有限公司 | 高纯度质粒dna制备物及其制备方法 |
| DE102008029356A1 (de) * | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| KR20110101143A (ko) * | 2008-11-04 | 2011-09-15 | 블러드 셀 스토리지 인코퍼레이티드 | 굴곡 유리 표면 상에서의 핵산 추출 |
| DE102008061714A1 (de) | 2008-12-12 | 2010-06-17 | Siemens Healthcare Diagnostics Inc., Deerfield | Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| GB2477752A (en) * | 2010-02-11 | 2011-08-17 | Arab Biotechnology Company | Detection of bacteria |
| US11274292B2 (en) | 2011-03-29 | 2022-03-15 | Phynexus, Inc. | Devices and methods for plasmid purification |
| WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| CA3111723A1 (en) | 2012-01-30 | 2013-08-08 | Exact Sciences Development Company, Llc | Sulfonated small dna compositions and methods for sulfonating and desulfonating small dnas |
| US9206469B2 (en) * | 2012-07-18 | 2015-12-08 | Zymo Research Corporation | Nucleic acid purification |
| EP3054008A4 (en) * | 2013-10-03 | 2017-04-12 | Kyowa Hakko Bio Co., Ltd. | Method for purifying double-stranded ribonucleic acid |
| CN104370997B (zh) * | 2014-09-24 | 2018-07-31 | 陈辉 | 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法 |
| DK3906982T4 (da) | 2020-05-08 | 2025-11-10 | Axagarius Gmbh & Co Kg | Fremgangsmåde til plasmidrengøring under samtidig reduktion af endotoksiner |
| CN116157519A (zh) | 2020-07-29 | 2023-05-23 | 精密科学公司 | 经磺化dna的纯化 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3897309A (en) | 1974-02-15 | 1975-07-29 | Merck & Co Inc | Process for removing pyrogenic material from aqueous solutions |
| US4059512A (en) | 1974-12-27 | 1977-11-22 | Preventive Systems, Inc. | Process for removing endotoxin from biological fluids |
| US4491660A (en) | 1980-01-10 | 1985-01-01 | Abbott Laboratories | Matrix polymers for binding endotoxins |
| US4866034A (en) * | 1982-05-26 | 1989-09-12 | Ribi Immunochem Research Inc. | Refined detoxified endotoxin |
| JPH0738880B2 (ja) | 1983-06-24 | 1995-05-01 | 東レ株式会社 | エンドトキシン血症治療剤 |
| KR940005589B1 (ko) | 1986-04-02 | 1994-06-21 | 다이닛뽄세이야꾸 가부시끼가이샤 | 핵산 또는 엔도톡신의 제거제 및 제거방법 |
| DK255887D0 (da) | 1987-05-20 | 1987-05-20 | Claus Koch | Immunoassay |
| US4808314A (en) | 1987-09-18 | 1989-02-28 | Scripps Clinic And Research Foundation | Method for reducing bacterial endotoxin contamination in solutions of macromolecules |
| US5169535A (en) | 1989-09-22 | 1992-12-08 | Kurita Water Industries Ltd. | Method of removing endotoxin |
| US5059527A (en) | 1989-12-08 | 1991-10-22 | White David C | Detection of endotoxins of gram negative bacteria |
| US5371186A (en) | 1991-02-11 | 1994-12-06 | Biosynth S.R.L. | Synthetic peptides for detoxification of bacterial endotoxins and for the prevention and treatment of septic shock |
| US5346994A (en) | 1992-01-28 | 1994-09-13 | Piotr Chomczynski | Shelf-stable product and process for isolating RNA, DNA and proteins |
| DE69324716T2 (de) | 1992-02-13 | 1999-09-09 | Becton Dickinson And Co. | Celithydrat und Reinigung von DNS |
| DE4331358A1 (de) | 1992-10-12 | 1994-04-14 | Braun Melsungen Ag | Verfahren zur quantitativen selektiven Entfernung oder präparativen Gewinnung von Tumor-Nekrose-Faktor (TNF) oder/und Lipopolysacchariden (LPS) aus wäßrigen Flüssigkeiten |
| CA2170604C (en) | 1993-08-30 | 2007-03-13 | Vikas V. Padhye | Nucleic acid purification compositions and methods |
| US5503816A (en) | 1993-09-27 | 1996-04-02 | Becton Dickinson And Company | Silicate compounds for DNA purification |
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| US5990301A (en) * | 1994-02-07 | 1999-11-23 | Qiagen Gmbh | Process for the separation and purification of nucleic acids from biological sources |
| DE59505786D1 (de) * | 1994-02-07 | 1999-06-02 | Qiagen Gmbh | Verfahren zur abreicherung oder entfernung von endotoxinen |
| US5576185A (en) | 1994-04-15 | 1996-11-19 | Coulter Corporation | Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation |
| DE19520398B4 (de) | 1995-06-08 | 2009-04-16 | Roche Diagnostics Gmbh | Magnetisches Pigment |
| JP2965131B2 (ja) * | 1995-07-07 | 1999-10-18 | 東洋紡績株式会社 | 核酸結合用磁性担体およびそれを用いる核酸単離方法 |
| GB9604921D0 (en) * | 1996-03-08 | 1996-05-08 | Nat Blood Authority | Purification method |
| US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
-
1998
- 1998-04-22 US US09/064,449 patent/US6194562B1/en not_active Expired - Fee Related
-
1999
- 1999-04-22 WO PCT/US1999/008491 patent/WO1999054340A1/en not_active Ceased
- 1999-04-22 JP JP2000544678A patent/JP2002512252A/ja active Pending
- 1999-04-22 CA CA002329067A patent/CA2329067C/en not_active Expired - Fee Related
- 1999-04-22 AU AU36513/99A patent/AU740145B2/en not_active Ceased
- 1999-04-22 EP EP99918650A patent/EP1071695A4/en not_active Withdrawn
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