AU740145B2 - Endotoxin reduction in nucleic acid purification - Google Patents
Endotoxin reduction in nucleic acid purification Download PDFInfo
- Publication number
- AU740145B2 AU740145B2 AU36513/99A AU3651399A AU740145B2 AU 740145 B2 AU740145 B2 AU 740145B2 AU 36513/99 A AU36513/99 A AU 36513/99A AU 3651399 A AU3651399 A AU 3651399A AU 740145 B2 AU740145 B2 AU 740145B2
- Authority
- AU
- Australia
- Prior art keywords
- solution
- silica
- matrix
- nucleic acid
- endotoxins
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
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- XMBWDFGMSWQBCA-UHFFFAOYSA-N hydrogen iodide Chemical compound I XMBWDFGMSWQBCA-UHFFFAOYSA-N 0.000 description 1
- SZVJSHCCFOBDDC-UHFFFAOYSA-N iron(II,III) oxide Inorganic materials O=[Fe]O[Fe]O[Fe]=O SZVJSHCCFOBDDC-UHFFFAOYSA-N 0.000 description 1
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- VLTRZXGMWDSKGL-UHFFFAOYSA-N perchloric acid Chemical compound OCl(=O)(=O)=O VLTRZXGMWDSKGL-UHFFFAOYSA-N 0.000 description 1
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- 229910001488 sodium perchlorate Inorganic materials 0.000 description 1
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/064,449 US6194562B1 (en) | 1998-04-22 | 1998-04-22 | Endotoxin reduction in nucleic acid purification |
| US09/064449 | 1998-04-22 | ||
| PCT/US1999/008491 WO1999054340A1 (en) | 1998-04-22 | 1999-04-22 | Endotoxin reduction in nucleic acid purification |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| AU3651399A AU3651399A (en) | 1999-11-08 |
| AU740145B2 true AU740145B2 (en) | 2001-11-01 |
Family
ID=22056061
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU36513/99A Ceased AU740145B2 (en) | 1998-04-22 | 1999-04-22 | Endotoxin reduction in nucleic acid purification |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US6194562B1 (enExample) |
| EP (1) | EP1071695A4 (enExample) |
| JP (1) | JP2002512252A (enExample) |
| AU (1) | AU740145B2 (enExample) |
| CA (1) | CA2329067C (enExample) |
| WO (1) | WO1999054340A1 (enExample) |
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| DE59505786D1 (de) * | 1994-02-07 | 1999-06-02 | Qiagen Gmbh | Verfahren zur abreicherung oder entfernung von endotoxinen |
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| US7078224B1 (en) * | 1999-05-14 | 2006-07-18 | Promega Corporation | Cell concentration and lysate clearance using paramagnetic particles |
| WO2000044928A2 (en) * | 1999-01-27 | 2000-08-03 | Halaka Folim G | Materials and methods for the purification of polyelectrolytes |
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| WO2002000342A2 (en) * | 2000-06-23 | 2002-01-03 | Irm, Llc | Method and apparatus for performing an assay |
| DE10033991A1 (de) * | 2000-07-12 | 2002-01-24 | Qiagen Gmbh | Verfahren zur Isolierung von Nukleinsäuren |
| US20030228600A1 (en) * | 2000-07-14 | 2003-12-11 | Eppendorf 5 Prime, Inc. | DNA isolation method and kit |
| WO2002044414A2 (en) * | 2000-11-28 | 2002-06-06 | Promega Corporation | Purification of dna sequencing reactions using silica magnetic particles |
| KR100463415B1 (ko) * | 2001-12-12 | 2004-12-23 | 씨제이 주식회사 | 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법 |
| AU2003202026A1 (en) | 2002-01-16 | 2003-09-02 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
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| WO2005012487A2 (en) * | 2003-08-01 | 2005-02-10 | Invitrogen Corporation | Compositions and methods for preparing short rna molecules and other nucleic acids |
| EP1709197A4 (en) * | 2003-12-30 | 2007-07-04 | Sigma Aldrich Co | RAPID PREPARATION OF NUCLEIC ACIDS BY ENZYMATIC DEPENDENCE |
| US7531308B2 (en) * | 2004-04-23 | 2009-05-12 | Sigma-Aldrich Co. | Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography |
| JP2008510456A (ja) | 2004-07-30 | 2008-04-10 | アジェンコート バイオサイエンス コーポレーション | 多官能基コート化固相担体を用いる核酸の単離方法 |
| US20060166223A1 (en) * | 2005-01-26 | 2006-07-27 | Reed Michael W | DNA purification and analysis on nanoengineered surfaces |
| JP2009500019A (ja) * | 2005-07-01 | 2009-01-08 | プロメガ・コーポレーション | 生体分子の精製のための浮遊性粒子のネットワーク、及び生体分子の精製のための浮遊性粒子又は浮遊性粒子のネットワークの使用 |
| DE102005059217B4 (de) * | 2005-12-07 | 2011-03-17 | Aj Innuscreen Gmbh | Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren |
| EP1963526A4 (en) | 2005-12-09 | 2009-11-18 | Promega Corp | PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX |
| CN101091797B (zh) * | 2006-06-23 | 2012-08-01 | 上海海规生物科技有限公司 | 去除初纯质粒或蛋白质溶液中内毒素的方法及试剂盒 |
| US8163535B2 (en) * | 2006-06-26 | 2012-04-24 | Blood Cell Storage, Inc. | Devices and processes for nucleic acid extraction |
| US7608399B2 (en) * | 2006-06-26 | 2009-10-27 | Blood Cell Storage, Inc. | Device and method for extraction and analysis of nucleic acids from biological samples |
| CN103255130B (zh) * | 2008-04-30 | 2015-12-23 | 斯特莱科生物有限公司 | 高纯度质粒dna制备物及其制备方法 |
| DE102008029356A1 (de) * | 2008-06-20 | 2009-12-24 | Siemens Healthcare Diagnostics Gmbh | Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe |
| KR20110101143A (ko) * | 2008-11-04 | 2011-09-15 | 블러드 셀 스토리지 인코퍼레이티드 | 굴곡 유리 표면 상에서의 핵산 추출 |
| DE102008061714A1 (de) | 2008-12-12 | 2010-06-17 | Siemens Healthcare Diagnostics Inc., Deerfield | Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe |
| US8039613B2 (en) | 2009-08-28 | 2011-10-18 | Promega Corporation | Methods of purifying a nucleic acid and formulation and kit for use in performing such methods |
| US8222397B2 (en) * | 2009-08-28 | 2012-07-17 | Promega Corporation | Methods of optimal purification of nucleic acids and kit for use in performing such methods |
| GB2477752A (en) * | 2010-02-11 | 2011-08-17 | Arab Biotechnology Company | Detection of bacteria |
| US11274292B2 (en) | 2011-03-29 | 2022-03-15 | Phynexus, Inc. | Devices and methods for plasmid purification |
| WO2012159063A2 (en) | 2011-05-19 | 2012-11-22 | Blood Cell Strorage, Inc. | Gravity flow fluidic device for nucleic acid extraction |
| CA3111723A1 (en) | 2012-01-30 | 2013-08-08 | Exact Sciences Development Company, Llc | Sulfonated small dna compositions and methods for sulfonating and desulfonating small dnas |
| US9206469B2 (en) * | 2012-07-18 | 2015-12-08 | Zymo Research Corporation | Nucleic acid purification |
| EP3054008A4 (en) * | 2013-10-03 | 2017-04-12 | Kyowa Hakko Bio Co., Ltd. | Method for purifying double-stranded ribonucleic acid |
| CN104370997B (zh) * | 2014-09-24 | 2018-07-31 | 陈辉 | 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法 |
| DK3906982T4 (da) | 2020-05-08 | 2025-11-10 | Axagarius Gmbh & Co Kg | Fremgangsmåde til plasmidrengøring under samtidig reduktion af endotoksiner |
| CN116157519A (zh) | 2020-07-29 | 2023-05-23 | 精密科学公司 | 经磺化dna的纯化 |
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| US5981235A (en) * | 1996-07-29 | 1999-11-09 | Promega Corporation | Methods for isolating nucleic acids using alkaline protease |
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1998
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1999
- 1999-04-22 WO PCT/US1999/008491 patent/WO1999054340A1/en not_active Ceased
- 1999-04-22 JP JP2000544678A patent/JP2002512252A/ja active Pending
- 1999-04-22 CA CA002329067A patent/CA2329067C/en not_active Expired - Fee Related
- 1999-04-22 AU AU36513/99A patent/AU740145B2/en not_active Ceased
- 1999-04-22 EP EP99918650A patent/EP1071695A4/en not_active Withdrawn
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| Publication number | Publication date |
|---|---|
| CA2329067C (en) | 2003-08-12 |
| EP1071695A4 (en) | 2004-11-24 |
| US6194562B1 (en) | 2001-02-27 |
| WO1999054340A1 (en) | 1999-10-28 |
| CA2329067A1 (en) | 1999-10-28 |
| EP1071695A1 (en) | 2001-01-31 |
| AU3651399A (en) | 1999-11-08 |
| JP2002512252A (ja) | 2002-04-23 |
| WO1999054340A8 (en) | 1999-12-09 |
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