CA2329067C - Endotoxin reduction in nucleic acid purification - Google Patents

Endotoxin reduction in nucleic acid purification Download PDF

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Publication number
CA2329067C
CA2329067C CA002329067A CA2329067A CA2329067C CA 2329067 C CA2329067 C CA 2329067C CA 002329067 A CA002329067 A CA 002329067A CA 2329067 A CA2329067 A CA 2329067A CA 2329067 C CA2329067 C CA 2329067C
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CA
Canada
Prior art keywords
solution
silica
matrix
nucleic acid
endotoxins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002329067A
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English (en)
French (fr)
Other versions
CA2329067A1 (en
Inventor
Craig E. Smith
Donald A. Creswell
Rex M. Bitner
Douglas H. White
Braeden L. Butler
Scott A. Lesley
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Promega Corp
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Promega Corp
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Publication date
Application filed by Promega Corp filed Critical Promega Corp
Publication of CA2329067A1 publication Critical patent/CA2329067A1/en
Application granted granted Critical
Publication of CA2329067C publication Critical patent/CA2329067C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
CA002329067A 1998-04-22 1999-04-22 Endotoxin reduction in nucleic acid purification Expired - Fee Related CA2329067C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/064,449 1998-04-22
US09/064,449 US6194562B1 (en) 1998-04-22 1998-04-22 Endotoxin reduction in nucleic acid purification
PCT/US1999/008491 WO1999054340A1 (en) 1998-04-22 1999-04-22 Endotoxin reduction in nucleic acid purification

Publications (2)

Publication Number Publication Date
CA2329067A1 CA2329067A1 (en) 1999-10-28
CA2329067C true CA2329067C (en) 2003-08-12

Family

ID=22056061

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002329067A Expired - Fee Related CA2329067C (en) 1998-04-22 1999-04-22 Endotoxin reduction in nucleic acid purification

Country Status (6)

Country Link
US (1) US6194562B1 (enExample)
EP (1) EP1071695A4 (enExample)
JP (1) JP2002512252A (enExample)
AU (1) AU740145B2 (enExample)
CA (1) CA2329067C (enExample)
WO (1) WO1999054340A1 (enExample)

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US7078224B1 (en) * 1999-05-14 2006-07-18 Promega Corporation Cell concentration and lysate clearance using paramagnetic particles
AU2862100A (en) * 1999-01-27 2000-08-18 Folim G. Halaka Materials and methods for the purification of polyelectrolytes
DE19903507A1 (de) * 1999-01-29 2000-08-10 Roche Diagnostics Gmbh Verfahren zur Herstellung endotoxinfreier oder an Endotoxin abgereicherter Nukleinsäuren und deren Verwendung
AU2001276830A1 (en) * 2000-06-23 2002-01-08 Irm, Llc Method and apparatus for performing an assay
DE10033991A1 (de) * 2000-07-12 2002-01-24 Qiagen Gmbh Verfahren zur Isolierung von Nukleinsäuren
US20030228600A1 (en) * 2000-07-14 2003-12-11 Eppendorf 5 Prime, Inc. DNA isolation method and kit
EP1339876A2 (en) * 2000-11-28 2003-09-03 Promega Corporation Purification of dna sequencing reactions using silica magnetic particles
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
CA2473376A1 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US7183104B1 (en) 2002-08-23 2007-02-27 Duane Morris Llp Separator and particle detection system
WO2005012487A2 (en) * 2003-08-01 2005-02-10 Invitrogen Corporation Compositions and methods for preparing short rna molecules and other nucleic acids
WO2005068662A1 (en) * 2003-12-30 2005-07-28 Sigma-Aldrich Co. Rapid preparation of nucleic acids by enzymatic digestion
US7531308B2 (en) * 2004-04-23 2009-05-12 Sigma-Aldrich Co. Process for the reduction of endotoxins in a plasmid preparation using a carbohydrate non-ionic detergent with silica chromatography
CA2575140A1 (en) 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
US20060166223A1 (en) * 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
CA2613094A1 (en) * 2005-07-01 2007-01-11 Promega Corporation Network of buoyant particles for biomolecule purification
DE102005059217B4 (de) 2005-12-07 2011-03-17 Aj Innuscreen Gmbh Verfahren und Testkit zur Trennung, Aufreinigung und Wiedergewinnung von lang- und kurzkettigen Nukleinsäuren
EP1963526A4 (en) * 2005-12-09 2009-11-18 Promega Corp PURIFICATION OF NUCLEIC ACID WITH A BINDING MATRIX
CN101091797B (zh) * 2006-06-23 2012-08-01 上海海规生物科技有限公司 去除初纯质粒或蛋白质溶液中内毒素的方法及试剂盒
US7608399B2 (en) * 2006-06-26 2009-10-27 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
WO2009117167A1 (en) * 2008-01-02 2009-09-24 Blood Cell Storage, Inc. Devices and processes for nucleic acid extraction
CN102171341A (zh) * 2008-04-30 2011-08-31 格兰达利斯有限公司 高纯度质粒dna制备物及其制备方法
DE102008029356A1 (de) * 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
CN102264899A (zh) * 2008-11-04 2011-11-30 血细胞保存公司 弯曲的玻璃表面上的核酸提取
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US8039613B2 (en) 2009-08-28 2011-10-18 Promega Corporation Methods of purifying a nucleic acid and formulation and kit for use in performing such methods
US8222397B2 (en) * 2009-08-28 2012-07-17 Promega Corporation Methods of optimal purification of nucleic acids and kit for use in performing such methods
GB2477752A (en) * 2010-02-11 2011-08-17 Arab Biotechnology Company Detection of bacteria
US11274292B2 (en) 2011-03-29 2022-03-15 Phynexus, Inc. Devices and methods for plasmid purification
WO2012159063A2 (en) 2011-05-19 2012-11-22 Blood Cell Strorage, Inc. Gravity flow fluidic device for nucleic acid extraction
CA2863215C (en) 2012-01-30 2021-05-04 Exact Sciences Corporation Modification of dna on magnetic beads
US9206469B2 (en) * 2012-07-18 2015-12-08 Zymo Research Corporation Nucleic acid purification
WO2015050191A1 (ja) * 2013-10-03 2015-04-09 協和発酵バイオ株式会社 二重鎖リボ核酸の精製方法
CN104370997B (zh) * 2014-09-24 2018-07-31 陈辉 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法
EP3906982B2 (de) 2020-05-08 2025-08-13 AXAGARIUS GmbH & Co. KG Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen
CA3186515A1 (en) 2020-07-29 2022-02-24 Evan M. RAGLAND Purification of sulfonated dna

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US3897309A (en) 1974-02-15 1975-07-29 Merck & Co Inc Process for removing pyrogenic material from aqueous solutions
US4059512A (en) 1974-12-27 1977-11-22 Preventive Systems, Inc. Process for removing endotoxin from biological fluids
US4491660A (en) 1980-01-10 1985-01-01 Abbott Laboratories Matrix polymers for binding endotoxins
US4866034A (en) * 1982-05-26 1989-09-12 Ribi Immunochem Research Inc. Refined detoxified endotoxin
JPH0738880B2 (ja) 1983-06-24 1995-05-01 東レ株式会社 エンドトキシン血症治療剤
KR940005589B1 (ko) 1986-04-02 1994-06-21 다이닛뽄세이야꾸 가부시끼가이샤 핵산 또는 엔도톡신의 제거제 및 제거방법
DK255887D0 (da) 1987-05-20 1987-05-20 Claus Koch Immunoassay
US4808314A (en) 1987-09-18 1989-02-28 Scripps Clinic And Research Foundation Method for reducing bacterial endotoxin contamination in solutions of macromolecules
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US5059527A (en) 1989-12-08 1991-10-22 White David C Detection of endotoxins of gram negative bacteria
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DE4331358A1 (de) 1992-10-12 1994-04-14 Braun Melsungen Ag Verfahren zur quantitativen selektiven Entfernung oder präparativen Gewinnung von Tumor-Nekrose-Faktor (TNF) oder/und Lipopolysacchariden (LPS) aus wäßrigen Flüssigkeiten
DE69433425T2 (de) 1993-08-30 2004-10-07 Promega Corp Zusammensetzungen und verfahren zur reinigung von nukleinsäuren
US5503816A (en) 1993-09-27 1996-04-02 Becton Dickinson And Company Silicate compounds for DNA purification
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
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DE59505786D1 (de) * 1994-02-07 1999-06-02 Qiagen Gmbh Verfahren zur abreicherung oder entfernung von endotoxinen
US5576185A (en) 1994-04-15 1996-11-19 Coulter Corporation Method of positive or negative selection of a population or subpopulation of a sample utilizing particles and gravity sedimentation
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JP2965131B2 (ja) * 1995-07-07 1999-10-18 東洋紡績株式会社 核酸結合用磁性担体およびそれを用いる核酸単離方法
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Also Published As

Publication number Publication date
AU3651399A (en) 1999-11-08
US6194562B1 (en) 2001-02-27
WO1999054340A8 (en) 1999-12-09
EP1071695A1 (en) 2001-01-31
CA2329067A1 (en) 1999-10-28
AU740145B2 (en) 2001-11-01
WO1999054340A1 (en) 1999-10-28
EP1071695A4 (en) 2004-11-24
JP2002512252A (ja) 2002-04-23

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