JP2002501102A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2002501102A5 JP2002501102A5 JP2000528627A JP2000528627A JP2002501102A5 JP 2002501102 A5 JP2002501102 A5 JP 2002501102A5 JP 2000528627 A JP2000528627 A JP 2000528627A JP 2000528627 A JP2000528627 A JP 2000528627A JP 2002501102 A5 JP2002501102 A5 JP 2002501102A5
- Authority
- JP
- Japan
- Prior art keywords
- dye
- reporter
- quencher
- compound
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000975 dye Substances 0.000 description 48
- 108091034117 Oligonucleotide Proteins 0.000 description 24
- 150000001875 compounds Chemical class 0.000 description 20
- 238000000034 method Methods 0.000 description 12
- 150000007523 nucleic acids Chemical group 0.000 description 8
- 108091028043 Nucleic acid sequence Proteins 0.000 description 7
- 125000000449 nitro group Chemical group [O-][N+](*)=O 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 5
- 125000003118 aryl group Chemical group 0.000 description 5
- 239000002751 oligonucleotide probe Substances 0.000 description 5
- 229910052739 hydrogen Inorganic materials 0.000 description 4
- 239000001257 hydrogen Substances 0.000 description 4
- 125000005647 linker group Chemical group 0.000 description 4
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- BZTDTCNHAFUJOG-UHFFFAOYSA-N 6-carboxyfluorescein Chemical group C12=CC=C(O)C=C2OC2=CC(O)=CC=C2C11OC(=O)C2=CC=C(C(=O)O)C=C21 BZTDTCNHAFUJOG-UHFFFAOYSA-N 0.000 description 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
- 125000005157 alkyl carboxy group Chemical group 0.000 description 2
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 2
- 150000002431 hydrogen Chemical class 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000001018 xanthene dye Substances 0.000 description 2
- 108010037497 3'-nucleotidase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical group [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 150000003973 alkyl amines Chemical class 0.000 description 1
- ZYGHJZDHTFUPRJ-UHFFFAOYSA-N benzo-alpha-pyrone Natural products C1=CC=C2OC(=O)C=CC2=C1 ZYGHJZDHTFUPRJ-UHFFFAOYSA-N 0.000 description 1
- 235000001671 coumarin Nutrition 0.000 description 1
- 150000004775 coumarins Chemical class 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 150000005002 naphthylamines Chemical class 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 239000001022 rhodamine dye Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 150000003732 xanthenes Chemical class 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/012,525 US6080868A (en) | 1998-01-23 | 1998-01-23 | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| US09/012,525 | 1998-01-23 | ||
| PCT/US1999/001163 WO1999037717A1 (en) | 1998-01-23 | 1999-01-19 | Asymmetric cyanine dye quenchers |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004114829A Division JP4471711B2 (ja) | 1998-01-23 | 2004-04-08 | 非対称シアニン色素クエンチャー |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2002501102A JP2002501102A (ja) | 2002-01-15 |
| JP2002501102A5 true JP2002501102A5 (https=) | 2005-11-17 |
| JP3946443B2 JP3946443B2 (ja) | 2007-07-18 |
Family
ID=21755376
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2000528627A Expired - Lifetime JP3946443B2 (ja) | 1998-01-23 | 1999-01-19 | 非対称シアニン色素クエンチャー |
| JP2004114829A Expired - Lifetime JP4471711B2 (ja) | 1998-01-23 | 2004-04-08 | 非対称シアニン色素クエンチャー |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2004114829A Expired - Lifetime JP4471711B2 (ja) | 1998-01-23 | 2004-04-08 | 非対称シアニン色素クエンチャー |
Country Status (8)
| Country | Link |
|---|---|
| US (5) | US6080868A (https=) |
| EP (1) | EP1049744B1 (https=) |
| JP (2) | JP3946443B2 (https=) |
| AT (1) | ATE212367T1 (https=) |
| AU (1) | AU740989B2 (https=) |
| CA (1) | CA2318160C (https=) |
| DE (1) | DE69900813T2 (https=) |
| WO (1) | WO1999037717A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8865904B2 (en) | 2005-05-11 | 2014-10-21 | Life Technologies Corporation | Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use |
Families Citing this family (101)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5801155A (en) * | 1995-04-03 | 1998-09-01 | Epoch Pharmaceuticals, Inc. | Covalently linked oligonucleotide minor grove binder conjugates |
| US6583168B1 (en) * | 1997-11-25 | 2003-06-24 | Applera Corporation | Sulfonated diarylrhodamine dyes |
| US5936087A (en) * | 1997-11-25 | 1999-08-10 | The Perkin-Elmer Corporation | Dibenzorhodamine dyes |
| US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| SE9902565D0 (sv) * | 1999-07-05 | 1999-07-05 | Jonas Karlsson | Probe for analysis of nucleic acids |
| US20070134686A1 (en) * | 1999-10-29 | 2007-06-14 | Stratagene California | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3' flap |
| US7625705B2 (en) * | 1999-10-29 | 2009-12-01 | Hologic, Inc. | Methods and compositions for detection of a target nucleic acid sequence utilizing a probe with a 3′ flap |
| JP2001204497A (ja) * | 2000-01-20 | 2001-07-31 | Japan Science & Technology Corp | 蛍光共鳴エネルギー移動を利用した、ｄｎａハイブリダイゼーション法によるｄｎａ検出方法 |
| TW572969B (en) * | 2000-02-10 | 2004-01-21 | Hayashibara Biochem Lab | Trimethine cyanine dye, light absorbent, light-resistant improver and optical recording medium containing same, and process for producing same |
| US6323337B1 (en) | 2000-05-12 | 2001-11-27 | Molecular Probes, Inc. | Quenching oligonucleotides |
| US6887664B2 (en) | 2000-06-06 | 2005-05-03 | Applera Corporation | Asynchronous primed PCR |
| GB0024351D0 (en) * | 2000-10-04 | 2000-11-22 | Amersham Pharm Biotech Uk Ltd | Dye-labelled peptide and method |
| AU1312502A (en) * | 2000-10-11 | 2002-04-22 | Pe Corp Ny | Fluorescent nucleobase conjugates having anionic linkers |
| EP1412517B1 (en) * | 2001-03-09 | 2006-11-29 | Boston Probes, Inc. | Methods, kits and compositions of combination oligomers |
| JP4676654B2 (ja) * | 2001-07-19 | 2011-04-27 | 富士フイルム株式会社 | 蛍光ヌクレオチド及びそれを用いた標識法 |
| US20030082547A1 (en) | 2001-08-27 | 2003-05-01 | Ewing Gregory J. | Non-fluorescent quencher compounds and biomolecular assays |
| US8323903B2 (en) * | 2001-10-12 | 2012-12-04 | Life Technologies Corporation | Antibody complexes and methods for immunolabeling |
| US20050069962A1 (en) * | 2001-10-12 | 2005-03-31 | Archer Robert M | Antibody complexes and methods for immunolabeling |
| US7166478B2 (en) * | 2002-03-12 | 2007-01-23 | Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. | Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses |
| CA2492048A1 (en) * | 2002-07-18 | 2004-01-29 | The Board Of Trustees Of The Leland Stanford Junior University | Detection of chemical ligation using fluorescence quenching leaving groups |
| US20040137469A1 (en) * | 2002-09-08 | 2004-07-15 | Casale Ralph A | Methods, compositions and libraries pertaining PNA dimer and PNA oligomer synthesis |
| AU2003275018B2 (en) | 2002-09-20 | 2009-10-01 | Integrated Dna Technologies, Inc. | Anthraquinone quencher dyes, their methods of preparation and use |
| BRPI0408967B8 (pt) | 2003-03-31 | 2021-07-27 | Hoffmann La Roche | kit e métodos para detecção de um ácido nucléico de vários membros do sorogrupo do vírus da encefalite japonesa em uma amostra biológica sob condições de hibridização rigorosas |
| US7052412B2 (en) * | 2003-05-07 | 2006-05-30 | Bonneau Michael D | Load transfer arm for putter |
| US7619059B2 (en) * | 2003-07-29 | 2009-11-17 | Life Technologies Corporation | Bimolecular optical probes |
| US7727752B2 (en) | 2003-07-29 | 2010-06-01 | Life Technologies Corporation | Kinase and phosphatase assays |
| CA2445420A1 (en) * | 2003-07-29 | 2005-01-29 | Invitrogen Corporation | Kinase and phosphatase assays |
| US7271265B2 (en) * | 2003-08-11 | 2007-09-18 | Invitrogen Corporation | Cyanine compounds and their application as quenching compounds |
| JP2007505315A (ja) * | 2003-09-12 | 2007-03-08 | インヴィトロジェン コーポレーション | 多重結合および活性アッセイ |
| US7282339B2 (en) * | 2003-09-17 | 2007-10-16 | Invitrogen Corporation | Competitive immunoassay |
| US20050118619A1 (en) * | 2003-09-22 | 2005-06-02 | Wensheng Xia | Dark quenchers for fluorescence resonance energy transfer (FRET) in bioassays |
| US7439341B2 (en) | 2003-11-14 | 2008-10-21 | Integrated Dna Technologies, Inc. | Fluorescence quenching azo dyes, their methods of preparation and use |
| US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
| US7446202B2 (en) | 2003-12-05 | 2008-11-04 | Molecular Probes, Inc. | Cyanine dye compounds |
| US20050186606A1 (en) * | 2004-02-11 | 2005-08-25 | Schroeder Benjamin G. | Methods and compositions for detecting nucleic acids |
| US8883486B2 (en) | 2004-05-04 | 2014-11-11 | Universite De Montreal | Arrestin biosensor |
| US7939251B2 (en) | 2004-05-06 | 2011-05-10 | Roche Molecular Systems, Inc. | SENP1 as a marker for cancer |
| WO2006014680A1 (en) | 2004-07-19 | 2006-02-09 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
| US7935479B2 (en) | 2004-07-19 | 2011-05-03 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
| US20060292558A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for protein assay diagnostics |
| US7846676B2 (en) * | 2004-07-19 | 2010-12-07 | Cell Biosciences, Inc. | Methods and devices for analyte detection |
| US20060292649A1 (en) * | 2004-07-19 | 2006-12-28 | Cell Biosciences Inc. | Methods and apparatus for reference lab diagnostics |
| US7767834B2 (en) * | 2004-08-13 | 2010-08-03 | Elitech Holding B.V. | Phosphonylated fluorescent dyes and conjugates |
| EP1781675B1 (en) | 2004-08-13 | 2014-03-26 | Epoch Biosciences, Inc. | Phosphonate fluorescent dyes and conjugates |
| US7910753B2 (en) * | 2004-09-10 | 2011-03-22 | Anaspec Incorporated | Cyanine dyes and their applications as luminescence quenching compounds |
| US8084260B2 (en) * | 2004-11-24 | 2011-12-27 | Applied Biosystems, Llc | Spectral calibration method and system for multiple instruments |
| US20060140203A1 (en) * | 2004-12-28 | 2006-06-29 | Sanjeev Jain | System and method for packet queuing |
| US20070015176A1 (en) * | 2005-02-18 | 2007-01-18 | Applera Corporation | Small nucleic acid detection probes and uses thereof |
| WO2006094200A2 (en) * | 2005-03-03 | 2006-09-08 | The Trustees Of Boston College | Method of obtaining a desired localization for cellular imaging with the use of peptidoconjugates |
| JP2008533167A (ja) | 2005-03-15 | 2008-08-21 | アプレラ コーポレイション | 分析物の検出のための、抗体の代替抗原系の使用 |
| US8021611B2 (en) * | 2005-04-09 | 2011-09-20 | ProteinSimple | Automated micro-volume assay system |
| EP2409980B1 (en) | 2005-04-14 | 2015-06-03 | Applied Biosystems, LLC | 3'modified oligonucleotides containing pseudoisocytosine nucleobase derivatives and applications thereof as primers or probes |
| EP1886145A2 (en) | 2005-05-03 | 2008-02-13 | Applera Corporation | Fluorescent detection system and dye set for use therewith |
| US20060286378A1 (en) * | 2005-05-23 | 2006-12-21 | Shivkumar Chiruvolu | Nanostructured composite particles and corresponding processes |
| US7737281B2 (en) * | 2005-05-24 | 2010-06-15 | Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. | Purine based fluorescent dyes |
| US8357801B2 (en) | 2005-05-24 | 2013-01-22 | Enzo Life Sciences, Inc. | Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits |
| US7569695B2 (en) * | 2005-05-24 | 2009-08-04 | Enzo Life Sciences, Inc. | Dyes for the detection or quantification of desirable target molecules |
| US20060292574A1 (en) * | 2005-05-26 | 2006-12-28 | Center for Disease Control, Department of Health, Taiwan, R.O.C. | Automated VNTR genotyping method |
| US8227621B2 (en) | 2005-06-30 | 2012-07-24 | Li-Cor, Inc. | Cyanine dyes and methods of use |
| US7630849B2 (en) * | 2005-09-01 | 2009-12-08 | Applied Biosystems, Llc | Method of automated calibration and diagnosis of laboratory instruments |
| CN100361990C (zh) * | 2005-09-06 | 2008-01-16 | 武汉大学 | 含吲哚基团的二阶非线性光学发色团及其制法 |
| US8945361B2 (en) * | 2005-09-20 | 2015-02-03 | ProteinSimple | Electrophoresis standards, methods and kits |
| AU2005234696B2 (en) | 2005-11-18 | 2012-02-16 | Phanos Technologies, Inc. | Fluorescent membrane intercalating probes and methods for their use |
| WO2007100392A2 (en) * | 2005-11-30 | 2007-09-07 | Biotium, Inc. | Enzyme substrate comprising a functional dye and associated technology and methods |
| WO2007106690A2 (en) | 2006-03-15 | 2007-09-20 | Siemens Healthcare Diagnostics Inc. | Degenerate nucleobase analogs |
| US9115389B2 (en) | 2006-06-30 | 2015-08-25 | Rosetta Genomics Ltd. | Method for detecting a target nucleic acid comprising two portions using probes having a first portion complementary to the first portion of the target nucleic acid and a second portion substantially complementary to the second portion of the target nucleic acid |
| CA2984820C (en) * | 2007-04-04 | 2021-12-07 | Ande Corporation | Plastic microfluidic separation and detection platforms |
| CN101343420B (zh) * | 2007-07-12 | 2013-10-16 | 大连理工大学 | 一类不对称菁类荧光染料 |
| US20090023156A1 (en) * | 2007-07-20 | 2009-01-22 | Voss Karl O | Methods and reagents for quantifying analytes |
| US8017338B2 (en) * | 2007-11-20 | 2011-09-13 | Life Technologies Corporation | Reversible di-nucleotide terminator sequencing |
| WO2009067632A1 (en) * | 2007-11-20 | 2009-05-28 | Applied Biosystems Inc. | Method of sequencing nucleic acids using elaborated nucleotide phosphorothiolate compounds |
| AU2009212165A1 (en) | 2008-02-07 | 2009-08-13 | Pacific Biosciences Of California, Inc. | CIS reactive oxygen quenchers integrated into linkers |
| US7943320B2 (en) * | 2008-12-30 | 2011-05-17 | Canon U.S. Life Sciences, Inc. | Unsymmetrical cyanine dyes for high resolution nucleic acid melting analysis |
| US8332007B2 (en) * | 2009-01-12 | 2012-12-11 | The Board Of Trustees Of The University Of Illinois | Quantitative three-dimensional mapping of oxygen tension |
| WO2010117420A2 (en) | 2009-03-30 | 2010-10-14 | Pacific Biosciences Of California, Inc. | Fret-labeled compounds and uses therefor |
| GB0915664D0 (en) | 2009-09-08 | 2009-10-07 | Enigma Diagnostics Ltd | Reaction method |
| US9506057B2 (en) | 2010-03-26 | 2016-11-29 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| AU2011230496B2 (en) | 2010-03-26 | 2015-09-17 | Integrated Dna Technologies, Inc. | Methods for enhancing nucleic acid hybridization |
| AU2011299233B2 (en) | 2010-09-07 | 2016-09-15 | Integrated Dna Technologies, Inc. | Modifications for antisense compounds |
| US8486311B1 (en) | 2012-03-21 | 2013-07-16 | Revision Military, Ltd. | Eyewear including catechol functionalized boron pyrromethene dye for neutralizing laser threat |
| US8968875B2 (en) | 2012-03-21 | 2015-03-03 | Larry Takiff | Eyewear including nitrophenyl functionalized boron pyrromethene dye for neutralizing laser threat |
| EP2769204B1 (en) | 2012-05-02 | 2016-02-17 | Charles River Laboratories, Inc. | Cell capture system and use thereof |
| AU2013256174B2 (en) | 2012-05-02 | 2017-03-02 | Charles River Laboratories, Inc. | Viability staining method |
| EP2780707B1 (en) | 2012-05-02 | 2016-02-24 | Charles River Laboratories, Inc. | Method of detecting viable cells in a cell sample |
| WO2016069922A1 (en) | 2014-10-29 | 2016-05-06 | Biotium, Inc. | Nucleic acid modifying agents and uses thereof |
| NL2013786B1 (en) * | 2014-11-13 | 2016-10-07 | Original G B V | Quenched coating. |
| US9957393B2 (en) | 2015-03-30 | 2018-05-01 | Enzo Biochem, Inc. | Monoazo dyes with cyclic amine as fluorescence quenchers |
| JP6967006B2 (ja) | 2016-01-15 | 2021-11-17 | クアンタポール， インコーポレイテッド | 低減されたバックグラウンドの光学に基づくナノポア分析 |
| BR112018073378A2 (pt) | 2016-05-20 | 2019-03-06 | Quantum-Si Incorporated | composições de nucleotídeos marcados e métodos para sequenciamento de ácidos nucleicos |
| AU2017312746A1 (en) * | 2016-08-19 | 2019-01-31 | Quantapore, Inc. | Optically-based nanopore sequencing using quenching agents |
| US10738346B2 (en) | 2017-03-09 | 2020-08-11 | Elitechgroup, Inc. | Nitrodiarylethenes as fluorescence quenchers for nucleic acid probes |
| US10677728B2 (en) | 2017-08-17 | 2020-06-09 | Elitechgroup B.V. | Duplex stabilizing fluorescence quenchers for nucleic acid probes |
| CN109053778A (zh) * | 2018-06-14 | 2018-12-21 | 忻州师范学院 | 特异性检测氰根的试剂及其合成方法和应用 |
| US20210310002A1 (en) * | 2018-08-31 | 2021-10-07 | City Of Hope | Cationic compounds for delivery of nucleic acids |
| JP7475445B2 (ja) * | 2019-11-19 | 2024-04-26 | エスエフシー カンパニー リミテッド | 消光子およびこの用途 |
| US20230075533A1 (en) | 2021-09-03 | 2023-03-09 | ProteinSimple | Methods and systems for analysis of samples containing particles used for gene delivery |
| JP7765627B2 (ja) | 2021-10-26 | 2025-11-06 | カリブー・バイオサイエンシーズ・インコーポレイテッド | エキソヌクレアーゼ連動式リアルタイムエンドヌクレアーゼ活性アッセイ |
| CN116003448B (zh) * | 2022-11-07 | 2024-06-11 | 淮阴工学院 | 一种含吲哚啉多烯键识别Aβ纤维的BODIPY近红外荧光探针及其制备方法 |
| EP4665402A1 (en) | 2023-02-17 | 2025-12-24 | Danmarks Tekniske Universitet | Copolymer-drug conjugate for treatment of tumours |
| CN119707923B (zh) * | 2023-09-28 | 2025-10-17 | 中国科学院大连化学物理研究所 | 一种衍生化试剂及其在鉴定氨基代谢物方法中的应用 |
| WO2026050087A1 (en) | 2024-08-27 | 2026-03-05 | Caribou Biosciences, Inc. | Real-time crispr endonuclease activity assay |
Family Cites Families (44)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2078233A (en) * | 1934-09-14 | 1937-04-27 | Eastman Kodak Co | Photographic emulsion |
| GB455710A (en) * | 1935-03-21 | 1936-10-21 | Kodak Ltd | Improvements in the manufacture of cyanine and related dyes |
| US2152615A (en) * | 1936-08-29 | 1939-03-28 | Agfa Ansco Corp | Pentamethine-omega-aldehydes of heterocyclic bases |
| US2269234A (en) | 1940-10-18 | 1942-01-06 | Eastman Kodak Co | Polymethine dye intermediates |
| US4230558A (en) | 1978-10-02 | 1980-10-28 | Coulter Electronics, Inc. | Single drop separator |
| US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
| US4439356A (en) | 1981-03-03 | 1984-03-27 | Syva Company | Unsymmetrical fluorescein derivatives |
| US4415732A (en) | 1981-03-27 | 1983-11-15 | University Patents, Inc. | Phosphoramidite compounds and processes |
| US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
| CA1190838A (en) | 1981-07-17 | 1985-07-23 | Cavit Akin | Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US5258538A (en) | 1985-08-26 | 1993-11-02 | Applied Biosystems, Inc. | 2,3-disubstituted-1,3,2-oxazaphosphacycloalkanes as nucleic acid linking agents |
| US4883867A (en) * | 1985-11-01 | 1989-11-28 | Becton, Dickinson And Company | Detection of reticulocytes, RNA or DNA |
| EP0232967B1 (en) | 1986-01-10 | 1993-04-28 | Amoco Corporation | Competitive homogeneous assay |
| US4855225A (en) | 1986-02-07 | 1989-08-08 | Applied Biosystems, Inc. | Method of detecting electrophoretically separated oligonucleotides |
| US4811218A (en) | 1986-06-02 | 1989-03-07 | Applied Biosystems, Inc. | Real time scanning electrophoresis apparatus for DNA sequencing |
| JP2527340B2 (ja) | 1986-12-15 | 1996-08-21 | アプライド バイオシステムズ インコーポレイテッド | ロ―ダミン染料の５−及び６−スクシニミジルカルボキシレ―ト異性体 |
| US5231191A (en) | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
| US5188934A (en) | 1989-11-14 | 1993-02-23 | Applied Biosystems, Inc. | 4,7-dichlorofluorescein dyes as molecular probes |
| US5994056A (en) | 1991-05-02 | 1999-11-30 | Roche Molecular Systems, Inc. | Homogeneous methods for nucleic acid amplification and detection |
| WO1993006482A1 (en) * | 1991-09-16 | 1993-04-01 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes |
| US5321130A (en) * | 1992-02-10 | 1994-06-14 | Molecular Probes, Inc. | Unsymmetrical cyanine dyes with a cationic side chain |
| EP0601889A2 (en) | 1992-12-10 | 1994-06-15 | Maine Medical Center Research Institute | Nucleic acid probes |
| US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
| US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
| US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
| US5658751A (en) * | 1993-04-13 | 1997-08-19 | Molecular Probes, Inc. | Substituted unsymmetrical cyanine dyes with selected permeability |
| US5538848A (en) * | 1994-11-16 | 1996-07-23 | Applied Biosystems Division, Perkin-Elmer Corp. | Method for detecting nucleic acid amplification using self-quenching fluorescence probe |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| US5597696A (en) * | 1994-07-18 | 1997-01-28 | Becton Dickinson And Company | Covalent cyanine dye oligonucleotide conjugates |
| US5565554A (en) * | 1994-07-29 | 1996-10-15 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
| JP3189000B2 (ja) * | 1994-12-01 | 2001-07-16 | 東ソー株式会社 | 特定核酸配列の検出方法 |
| US5656449A (en) * | 1995-03-06 | 1997-08-12 | Molecular Probes, Inc. | Neutral unsymmetrical cyanine dyes |
| EP0826066B1 (en) | 1995-05-05 | 2000-09-13 | The Perkin-Elmer Corporation | Methods and reagents for combined pcr amplification and hybridization probing assay |
| US5658735A (en) * | 1995-11-09 | 1997-08-19 | Biometric Imaging, Inc. | Cyclized fluorescent nucleic acid intercalating cyanine dyes and nucleic acid detection methods |
| JPH09288326A (ja) * | 1996-04-23 | 1997-11-04 | Fuji Photo Film Co Ltd | 熱現像感光材料 |
| US5847162A (en) | 1996-06-27 | 1998-12-08 | The Perkin Elmer Corporation | 4, 7-Dichlororhodamine dyes |
| US5863727A (en) | 1996-05-03 | 1999-01-26 | The Perkin-Elmer Corporation | Energy transfer dyes with enhanced fluorescence |
| SE506700C2 (sv) * | 1996-05-31 | 1998-02-02 | Mikael Kubista | Sond och förfaranden för analys av nukleinsyra |
| US5736333A (en) | 1996-06-04 | 1998-04-07 | The Perkin-Elmer Corporation | Passive internal references for the detection of nucleic acid amplification products |
| US5821356A (en) | 1996-08-12 | 1998-10-13 | The Perkin Elmer Corporation | Propargylethoxyamino nucleotides |
| US5770716A (en) | 1997-04-10 | 1998-06-23 | The Perkin-Elmer Corporation | Substituted propargylethoxyamido nucleosides, oligonucleotides and methods for using same |
| US6080868A (en) | 1998-01-23 | 2000-06-27 | The Perkin-Elmer Corporation | Nitro-substituted non-fluorescent asymmetric cyanine dye compounds |
| JP2003501540A (ja) | 1999-06-09 | 2003-01-14 | カーネギー メロン ユニヴァーシティ | 反応性蛍光試薬としてのｐＨ官能性シアニン染料 |
-
1998
- 1998-01-23 US US09/012,525 patent/US6080868A/en not_active Expired - Lifetime
-
1999
- 1999-01-19 AT AT99902392T patent/ATE212367T1/de not_active IP Right Cessation
- 1999-01-19 CA CA002318160A patent/CA2318160C/en not_active Expired - Fee Related
- 1999-01-19 EP EP99902392A patent/EP1049744B1/en not_active Expired - Lifetime
- 1999-01-19 DE DE69900813T patent/DE69900813T2/de not_active Expired - Lifetime
- 1999-01-19 AU AU22383/99A patent/AU740989B2/en not_active Ceased
- 1999-01-19 WO PCT/US1999/001163 patent/WO1999037717A1/en not_active Ceased
- 1999-01-19 JP JP2000528627A patent/JP3946443B2/ja not_active Expired - Lifetime
- 1999-07-20 US US09/357,740 patent/US6348596B1/en not_active Expired - Lifetime
-
2000
- 2000-06-21 US US09/602,544 patent/US6541618B1/en not_active Expired - Lifetime
-
2003
- 2003-03-31 US US10/404,404 patent/US6750024B2/en not_active Expired - Lifetime
-
2004
- 2004-04-08 JP JP2004114829A patent/JP4471711B2/ja not_active Expired - Lifetime
- 2004-05-05 US US10/840,189 patent/US7166715B2/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8865904B2 (en) | 2005-05-11 | 2014-10-21 | Life Technologies Corporation | Fluorescent chemical compounds having high selectivity for double stranded DNA, and methods for their use |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2002501102A5 (https=) | ||
| US4766064A (en) | Displacement polynucleotide assay employing polyether and diagnostic kit | |
| JP4471711B2 (ja) | 非対称シアニン色素クエンチャー | |
| EP0543942B2 (en) | Homogeneous assay system | |
| EP2496705B1 (en) | Sequence-specific methods for homogenous, real-time detection of lamp products | |
| DE69736667T2 (de) | Verfahren zum nachweis und amplifikation von nukleinsäuresequenzen unter verbrauch von modifizierten oligonukleotiden mit erhöhter zielschmelztemperatur (tm) | |
| EP1689764B1 (en) | Oligonucleotides labeled with a plurality of fluorophores | |
| CA2255531C (en) | Labeled primer for use in and detection of target nucleic acids | |
| ES2294862T5 (es) | Procedimiento de síntesis de ácido nucleico. | |
| EP1002877B1 (en) | Gene amplifying method | |
| CN103114131B (zh) | 一种引物中部序列干扰pcr技术 | |
| US9194007B2 (en) | Double-stranded probes for the fluorescent detection of nucleic acids | |
| EP0379369A2 (en) | Nucleic acid amplification using single primer | |
| JPH0437720B2 (https=) | ||
| WO1991006626A2 (en) | Triple helix formation in oligonucleotide therapy | |
| WO2000070095A2 (en) | Homogeneous isothermal amplification and detection of nucleic acids using a template switch oligonucleotide | |
| JP2009240332A (ja) | 移動度改変ヌクレオ塩基ポリマーおよび同一物を使用する方法 | |
| EP0726962A4 (en) | HIV PROBE FOR SOLUTION SANDWICH HIBRIDATION PROCESS | |
| JPH09511658A (ja) | 鳥型結核菌複合種の増幅および検出 | |
| CN106868111A (zh) | 利用通用TaqMan探针检测SNP的方法和试剂盒 | |
| US20160215334A1 (en) | Hybridization probes and methods | |
| Kumaresan et al. | Structure of the DNA interstrand crosslink of 4, 5', 8-trimethylpsoralen | |
| JP7391321B2 (ja) | アクネ菌のｄｎａタイプの判別用オリゴヌクレオチドセット、キット及びアクネ菌のｄｎａタイプの判別方法 | |
| US7135284B1 (en) | Primer extension methods for production of high specific activity nucleic acid probes | |
| Chowdhury et al. | Pseudo-dumbbell-type molecular beacon probes bearing modified deoxyuridine derivatives and a silylated pyrene as a fluorophore |