CA2318160C - Asymmetric cyanine dye quenchers - Google Patents

Asymmetric cyanine dye quenchers Download PDF

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Publication number
CA2318160C
CA2318160C CA002318160A CA2318160A CA2318160C CA 2318160 C CA2318160 C CA 2318160C CA 002318160 A CA002318160 A CA 002318160A CA 2318160 A CA2318160 A CA 2318160A CA 2318160 C CA2318160 C CA 2318160C
Authority
CA
Canada
Prior art keywords
dye
quencher
reporter
oligonucleotide
labelled oligonucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002318160A
Other languages
English (en)
French (fr)
Other versions
CA2318160A1 (en
Inventor
Linda G. Lee
Ronald J. Graham
Khairuzzaman B. Mullah
Francis T. Haxo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Applera Corp
Applied Biosystems LLC
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp, Applied Biosystems LLC, Applied Biosystems Inc filed Critical Applera Corp
Publication of CA2318160A1 publication Critical patent/CA2318160A1/en
Application granted granted Critical
Publication of CA2318160C publication Critical patent/CA2318160C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Engineering & Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA002318160A 1998-01-23 1999-01-19 Asymmetric cyanine dye quenchers Expired - Fee Related CA2318160C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US09/012525 1998-01-23
US09/012,525 US6080868A (en) 1998-01-23 1998-01-23 Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
PCT/US1999/001163 WO1999037717A1 (en) 1998-01-23 1999-01-19 Asymmetric cyanine dye quenchers

Publications (2)

Publication Number Publication Date
CA2318160A1 CA2318160A1 (en) 1999-07-29
CA2318160C true CA2318160C (en) 2005-11-29

Family

ID=21755376

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002318160A Expired - Fee Related CA2318160C (en) 1998-01-23 1999-01-19 Asymmetric cyanine dye quenchers

Country Status (8)

Country Link
US (5) US6080868A (https=)
EP (1) EP1049744B1 (https=)
JP (2) JP3946443B2 (https=)
AT (1) ATE212367T1 (https=)
AU (1) AU740989B2 (https=)
CA (1) CA2318160C (https=)
DE (1) DE69900813T2 (https=)
WO (1) WO1999037717A1 (https=)

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Publication number Publication date
US20030207305A1 (en) 2003-11-06
EP1049744B1 (en) 2002-01-23
US6541618B1 (en) 2003-04-01
AU2238399A (en) 1999-08-09
JP4471711B2 (ja) 2010-06-02
CA2318160A1 (en) 1999-07-29
AU740989B2 (en) 2001-11-22
US6348596B1 (en) 2002-02-19
US6080868A (en) 2000-06-27
US7166715B2 (en) 2007-01-23
US6750024B2 (en) 2004-06-15
DE69900813T2 (de) 2002-10-02
JP2004323839A (ja) 2004-11-18
EP1049744A1 (en) 2000-11-08
JP2002501102A (ja) 2002-01-15
JP3946443B2 (ja) 2007-07-18
ATE212367T1 (de) 2002-02-15
DE69900813D1 (de) 2002-03-14
US20050009058A1 (en) 2005-01-13
WO1999037717A1 (en) 1999-07-29

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