JP2001501527A - 改良されたろ過システム及び方法 - Google Patents
改良されたろ過システム及び方法Info
- Publication number
- JP2001501527A JP2001501527A JP10514893A JP51489398A JP2001501527A JP 2001501527 A JP2001501527 A JP 2001501527A JP 10514893 A JP10514893 A JP 10514893A JP 51489398 A JP51489398 A JP 51489398A JP 2001501527 A JP2001501527 A JP 2001501527A
- Authority
- JP
- Japan
- Prior art keywords
- filter
- filter basket
- basket
- vacuum
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000001914 filtration Methods 0.000 title claims abstract description 19
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 29
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 29
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 28
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 47
- 239000000463 material Substances 0.000 claims description 28
- 239000000377 silicon dioxide Substances 0.000 claims description 24
- 239000006166 lysate Substances 0.000 claims description 20
- 238000005119 centrifugation Methods 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 238000005406 washing Methods 0.000 claims description 5
- 230000027455 binding Effects 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 230000006978 adaptation Effects 0.000 claims 2
- 239000000126 substance Substances 0.000 claims 2
- 239000012149 elution buffer Substances 0.000 claims 1
- 230000002934 lysing effect Effects 0.000 claims 1
- 230000001737 promoting effect Effects 0.000 claims 1
- 108020004414 DNA Proteins 0.000 description 120
- 239000013612 plasmid Substances 0.000 description 96
- 239000000243 solution Substances 0.000 description 28
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 22
- 241000588724 Escherichia coli Species 0.000 description 20
- 239000007984 Tris EDTA buffer Substances 0.000 description 17
- 238000000746 purification Methods 0.000 description 17
- 230000001580 bacterial effect Effects 0.000 description 15
- 239000000203 mixture Substances 0.000 description 12
- 108091005658 Basic proteases Proteins 0.000 description 11
- 239000003242 anti bacterial agent Substances 0.000 description 11
- 229940088710 antibiotic agent Drugs 0.000 description 11
- 238000002955 isolation Methods 0.000 description 10
- 239000006228 supernatant Substances 0.000 description 10
- 230000000694 effects Effects 0.000 description 9
- 101150012763 endA gene Proteins 0.000 description 9
- 239000002244 precipitate Substances 0.000 description 9
- 238000012163 sequencing technique Methods 0.000 description 9
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000013049 sediment Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 238000003828 vacuum filtration Methods 0.000 description 6
- 102000004190 Enzymes Human genes 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 5
- 230000009089 cytolysis Effects 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 108091005804 Peptidases Proteins 0.000 description 4
- 239000004365 Protease Substances 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 239000000872 buffer Substances 0.000 description 4
- 238000010828 elution Methods 0.000 description 4
- 230000010076 replication Effects 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 229920000936 Agarose Polymers 0.000 description 3
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 description 3
- 239000005695 Ammonium acetate Substances 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 3
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 3
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 3
- 229940043376 ammonium acetate Drugs 0.000 description 3
- 235000019257 ammonium acetate Nutrition 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000004140 cleaning Methods 0.000 description 3
- 239000000356 contaminant Substances 0.000 description 3
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 3
- 229960005542 ethidium bromide Drugs 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 238000007399 DNA isolation Methods 0.000 description 2
- 108010042407 Endonucleases Proteins 0.000 description 2
- 102000004533 Endonucleases Human genes 0.000 description 2
- ZRALSGWEFCBTJO-UHFFFAOYSA-N Guanidine Chemical compound NC(N)=N ZRALSGWEFCBTJO-UHFFFAOYSA-N 0.000 description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 230000006037 cell lysis Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012869 ethanol precipitation Methods 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 229910052749 magnesium Inorganic materials 0.000 description 2
- 239000011777 magnesium Substances 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 229920002477 rna polymer Polymers 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000013382 DNA quantification Methods 0.000 description 1
- 230000004568 DNA-binding Effects 0.000 description 1
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 1
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 1
- 241001123946 Gaga Species 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- CHJJGSNFBQVOTG-UHFFFAOYSA-N N-methyl-guanidine Natural products CNC(N)=N CHJJGSNFBQVOTG-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- 241001600434 Plectroglyphidodon lacrymatus Species 0.000 description 1
- 101710141795 Ribonuclease inhibitor Proteins 0.000 description 1
- 229940122208 Ribonuclease inhibitor Drugs 0.000 description 1
- 102100037968 Ribonuclease inhibitor Human genes 0.000 description 1
- 108010056079 Subtilisins Proteins 0.000 description 1
- 102000005158 Subtilisins Human genes 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- SWSQBOPZIKWTGO-UHFFFAOYSA-N dimethylaminoamidine Natural products CN(C)C(N)=N SWSQBOPZIKWTGO-UHFFFAOYSA-N 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 230000002093 peripheral effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 239000003161 ribonuclease inhibitor Substances 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- -1 silica Nucleic acids Chemical class 0.000 description 1
- 230000003595 spectral effect Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
- 238000013024 troubleshooting Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5021—Test tubes specially adapted for centrifugation purposes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- General Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Clinical Laboratory Science (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Centrifugal Separators (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US2658296P | 1996-09-18 | 1996-09-18 | |
| US60/026,582 | 1996-09-18 | ||
| PCT/US1997/016634 WO1998011989A1 (en) | 1996-09-18 | 1997-09-18 | Improved filtration system and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JP2001501527A true JP2001501527A (ja) | 2001-02-06 |
Family
ID=21832621
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10514893A Ceased JP2001501527A (ja) | 1996-09-18 | 1997-09-18 | 改良されたろ過システム及び方法 |
Country Status (6)
| Country | Link |
|---|---|
| EP (1) | EP0927077A4 (ko) |
| JP (1) | JP2001501527A (ko) |
| KR (1) | KR100486402B1 (ko) |
| AU (1) | AU730718B2 (ko) |
| CA (1) | CA2265702A1 (ko) |
| WO (1) | WO1998011989A1 (ko) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106179075A (zh) * | 2015-04-30 | 2016-12-07 | 余家昌 | 一种物料分存式混合装置 |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2312102C (en) * | 1997-12-24 | 2007-09-04 | Cepheid | Integrated fluid manipulation cartridge |
| US6958392B2 (en) | 1998-10-09 | 2005-10-25 | Whatman, Inc. | Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products |
| US20020010323A1 (en) * | 1998-10-09 | 2002-01-24 | Andrew Martin Mitchell | Isolation method and apparatus |
| US7556733B2 (en) | 2001-06-15 | 2009-07-07 | Mds Analytical Technologies (Us) Inc. | Low volume filtration column devices and methods of filtering therewith |
| US7229595B2 (en) | 2001-06-15 | 2007-06-12 | Molecular Devices Corporation | Filtration column devices and methods of filtering therewith |
| US7749388B2 (en) | 2001-06-15 | 2010-07-06 | Life Technologies Corporation | Low volume filtration column devices and methods of filtering therewith |
| KR100454869B1 (ko) * | 2001-07-13 | 2004-11-03 | 주식회사 인트론바이오테크놀로지 | Ｄｎａ 추출용 세포 용해 버퍼 및 이를 이용한 ｄｎａ 추출방법 |
| US20030087293A1 (en) * | 2001-10-23 | 2003-05-08 | Decode Genetics Ehf. | Nucleic acid isolation method and apparatus for performing same |
| KR100486179B1 (ko) * | 2004-03-02 | 2005-05-03 | 피경태 | 핵산을 분리하고 정제하기 위한 세포 용해 조성물, 방법 및 키트 |
| KR101030848B1 (ko) | 2008-11-07 | 2011-04-22 | 연세대학교 산학협력단 | 동물조직의 실시간 유전자 추출 휴대장치 및 방법 |
| EP2260943A1 (en) | 2009-06-11 | 2010-12-15 | Innosieve Diagnostics B.V. | Microfilter centrifuge tube |
| KR20090129387A (ko) * | 2009-11-05 | 2009-12-16 | 주식회사 인트론바이오테크놀로지 | 생물학적 시료로부터의 생화학적 물질의 추출장치 |
| ES2358699B1 (es) | 2011-03-09 | 2012-03-14 | Zf Biotox, S.L. | Microplaca para ensayos biológicos. |
| EP2992958A1 (en) * | 2014-09-03 | 2016-03-09 | STAT-Diagnostica D Innovation SL | Nucleic acid purification cartridge |
| CN108554469B (zh) * | 2018-04-18 | 2020-06-30 | 中南大学湘雅医院 | 一种医药实验用的多功能试管装置 |
| US11591591B2 (en) * | 2019-08-21 | 2023-02-28 | New England Biolabs, Inc. | Isolation of high molecular weight DNA using beads |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4251366A (en) * | 1979-06-18 | 1981-02-17 | Simon Timothy M | Adapter for laboratory filter |
| US4675110A (en) * | 1986-01-31 | 1987-06-23 | Biomedical Polymers, Inc. | Filter device and apparatus with multiple gas return passages |
-
1997
- 1997-09-18 KR KR10-1999-7002337A patent/KR100486402B1/ko not_active IP Right Cessation
- 1997-09-18 WO PCT/US1997/016634 patent/WO1998011989A1/en active IP Right Grant
- 1997-09-18 JP JP10514893A patent/JP2001501527A/ja not_active Ceased
- 1997-09-18 AU AU44255/97A patent/AU730718B2/en not_active Ceased
- 1997-09-18 CA CA002265702A patent/CA2265702A1/en not_active Abandoned
- 1997-09-18 EP EP97942583A patent/EP0927077A4/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106179075A (zh) * | 2015-04-30 | 2016-12-07 | 余家昌 | 一种物料分存式混合装置 |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2265702A1 (en) | 1998-03-26 |
| EP0927077A1 (en) | 1999-07-07 |
| AU4425597A (en) | 1998-04-14 |
| AU730718B2 (en) | 2001-03-15 |
| EP0927077A4 (en) | 2000-07-19 |
| KR100486402B1 (ko) | 2005-04-29 |
| WO1998011989A1 (en) | 1998-03-26 |
| KR20000036247A (ko) | 2000-06-26 |
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