JP2000500342A - ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキット - Google Patents
ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキットInfo
- Publication number
- JP2000500342A JP2000500342A JP9519172A JP51917297A JP2000500342A JP 2000500342 A JP2000500342 A JP 2000500342A JP 9519172 A JP9519172 A JP 9519172A JP 51917297 A JP51917297 A JP 51917297A JP 2000500342 A JP2000500342 A JP 2000500342A
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- JP
- Japan
- Prior art keywords
- seq
- probe
- nucleic acid
- sequence
- oligonucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| PCT/US1996/018711 WO1997018334A2 (en) | 1995-11-15 | 1996-11-12 | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
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| JP2000500342A true JP2000500342A (ja) | 2000-01-18 |
| JP2000500342A5 JP2000500342A5 (enExample) | 2004-10-21 |
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| US (6) | US6583278B1 (enExample) |
| EP (1) | EP0774518A3 (enExample) |
| JP (1) | JP2000500342A (enExample) |
| AU (1) | AU726047B2 (enExample) |
| CA (1) | CA2237891C (enExample) |
| WO (1) | WO1997018334A2 (enExample) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004248678A (ja) * | 2000-09-26 | 2004-09-09 | National Institute Of Advanced Industrial & Technology | 新規核酸プローブ並びにそれを用いる核酸測定方法、及びその方法によって得られるデータを解析する方法 |
| JP2004267215A (ja) * | 2000-08-03 | 2004-09-30 | National Institute Of Advanced Industrial & Technology | 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法 |
| WO2007148762A1 (ja) * | 2006-06-22 | 2007-12-27 | Masahiko Kuroda | ヒトパピローマウイルス（ｈｐｖ）の検出方法、それに用いるプライマーセットおよび検出キット |
| JP2008524983A (ja) * | 2004-10-04 | 2008-07-17 | グッジェン インク | ヒトパピローマウイルスのプローブおよびそれを使用するｄｎａチップ |
| WO2020050350A1 (ja) * | 2018-09-07 | 2020-03-12 | 株式会社ヘルスケアシステムズ | ヒトパピローマウイルスの検出方法 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1997018334A2 (en) * | 1995-11-15 | 1997-05-22 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| US8048629B2 (en) | 1996-03-15 | 2011-11-01 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
| US6156504A (en) | 1996-03-15 | 2000-12-05 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays |
| US6511805B1 (en) * | 1996-03-15 | 2003-01-28 | The Penn State Research Foundation | Methods for detecting papillomavirus DNA in blood plasma and serum |
| BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
| JP4724380B2 (ja) * | 1999-04-20 | 2011-07-13 | 独立行政法人産業技術総合研究所 | 核酸の測定方法に用いる核酸プローブおよびデータを解析する方法 |
| KR100382703B1 (ko) | 2000-03-15 | 2003-05-09 | 주식회사 바이오메드랩 | 인유두종바이러스의 유전형 진단키트 및 상기 진단키트의제조방법 |
| US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| WO2003057914A2 (en) * | 2002-01-07 | 2003-07-17 | Norchip A/S | METHOD FOR DETECTING HUMAN PAPILLOMAVIRUS mRNA |
| GB0200258D0 (en) * | 2002-01-07 | 2002-02-20 | Norchip As | Detection of human papillomavirus |
| KR100452103B1 (ko) * | 2002-07-16 | 2004-10-08 | (주)차웰빙스 | 중합효소연쇄반응에 의한 종양원성 인유두종바이러스를검출하기 위한 프라이머와 종양원성 인유두종바이러스를검출하는 방법 |
| EP1439227A1 (en) * | 2003-01-15 | 2004-07-21 | Deutsches Krebsforschungszentrum Stiftung des öffentlichen Rechts | HPV-E6-specific siRNAs for the treatment of HPV-related tumors |
| JP4498357B2 (ja) * | 2003-09-25 | 2010-07-07 | サード・ウェーブ・テクノロジーズ・インク | Ｈｐｖの検出法 |
| EP1935992A3 (en) * | 2003-12-23 | 2008-12-03 | Autogenomics, Inc. | Multiplexed nucleic acid analysis with high specificity for human papilloma virus |
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| CN101730847B (zh) * | 2007-04-05 | 2015-03-18 | 分类生物系统有限公司 | 组合物及检测方法 |
| EP1997914A1 (en) * | 2007-06-01 | 2008-12-03 | Università Degli Studi Di Milano - Bicocca | Identification and quantification of oncogenic HPV nucleic acids by means of real-time PCR assays |
| JP2011518333A (ja) * | 2008-04-17 | 2011-06-23 | キアジェン ゲイサーズバーグ インコーポレイテッド | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット |
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Family Cites Families (51)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3687645T2 (de) | 1986-01-07 | 1993-05-27 | Gen Probe Inc | Verfahren der beschleunigten wiederassoziation der nukleinsaeure. |
| EP0272009B2 (en) | 1986-11-24 | 2007-10-03 | Gen-Probe Incorporated | Nucleic acid probes for detection and/or quantitation of non-viral organisms |
| US5079351A (en) | 1986-11-26 | 1992-01-07 | Cetus Corporation | Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization |
| ATE121794T1 (de) | 1987-02-26 | 1995-05-15 | Univ Sydney | Verfahren zum nachweis des karzinogenen menschlichen papillomavirus. |
| FR2629458B2 (fr) | 1987-07-31 | 1991-08-09 | Ire Celltarg Sa | Nouvelles sondes d'acides nucleiques specifiques de differents types de virus de papillome humain |
| KR970005898B1 (ko) | 1987-09-21 | 1997-04-21 | 젠- 프로우브 인코퍼레이티드 | 뉴클레오티드 프로브에 대한 비-뉴클레오티드 연결시약 |
| EP0338067A4 (en) | 1987-10-02 | 1991-05-02 | Microprobe Corporation | Human papillomavirus type diagnosis with nucleotide probes |
| US5185439A (en) | 1987-10-05 | 1993-02-09 | Gen-Probe Incorporated | Acridinium ester labelling and purification of nucleotide probes |
| PT88665B (pt) * | 1987-10-05 | 1992-12-31 | Ml Tecnology Ventures Lp | Metodo para a marcacao com ester de acridinio e purificacao de sondas nucleotidicas |
| US5030557A (en) | 1987-11-24 | 1991-07-09 | Ml Technology Venture | Means and method for enhancing nucleic acid hybridization |
| US4886741A (en) * | 1987-12-09 | 1989-12-12 | Microprobe Corporation | Use of volume exclusion agents for the enhancement of in situ hybridization |
| WO1989009940A1 (en) | 1988-04-04 | 1989-10-19 | Oncor, Inc. | Human papilloma virus typing method and nucleic acid probes used therein |
| US5182377A (en) | 1988-09-09 | 1993-01-26 | Hoffmann-La Roche Inc. | Probes for detection of human papillomavirus |
| US5447839A (en) | 1988-09-09 | 1995-09-05 | Hoffmann-La Roche Inc. | Detection of human papillomavirus by the polymerase chain reaction |
| US5639871A (en) | 1988-09-09 | 1997-06-17 | Roche Molecular Systems, Inc. | Detection of human papillomavirus by the polymerase chain reaction |
| SE8803870D0 (sv) | 1988-10-28 | 1988-10-28 | Medscand Ab | Method for detection of human papillomavirus (hpv) for diagnostic purposes |
| DE3838269A1 (de) | 1988-11-11 | 1990-05-17 | Behringwerke Ag | Nachweis humaner papillomavirus dna und ihrer expression in zervix-abstrichen |
| US4882243A (en) | 1988-11-22 | 1989-11-21 | Polytechnic University | Preparation of metallic cation conducting polymers based on sterically hindered phenols containing polymeric systems |
| JP2791685B2 (ja) * | 1989-06-08 | 1998-08-27 | 寳酒造株式会社 | パピローマウイルスの検出方法 |
| CA2020958C (en) | 1989-07-11 | 2005-01-11 | Daniel L. Kacian | Nucleic acid sequence amplification methods |
| US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
| AU6966991A (en) | 1989-12-01 | 1991-06-26 | Gene-Trak Systems | Detection of hpv transcripts |
| NL9000134A (nl) | 1990-01-19 | 1991-08-16 | Stichting Res Fonds Pathologie | Primers en werkwijze voor het detecteren van humaan papilloma virus genotypen m.b.v. pcr. |
| US5427930A (en) | 1990-01-26 | 1995-06-27 | Abbott Laboratories | Amplification of target nucleic acids using gap filling ligase chain reaction |
| GB9015845D0 (en) * | 1990-07-19 | 1990-09-05 | Emery Vincent C | Diagnostic method |
| JP2785164B2 (ja) * | 1990-08-20 | 1998-08-13 | 寳酒造株式会社 | ヒトパピローマウイルスの検出方法 |
| US5253171A (en) * | 1990-09-21 | 1993-10-12 | General Electric Company | Parallel processing method and apparatus based on the algebra reconstruction technique for reconstructing a three-dimensional computerized tomography (CT) image from cone beam projection data |
| CA2052413C (en) * | 1990-09-28 | 2004-07-13 | Jeffrey L. Joseph | Nucleotides sequences useful as type-specific probes, pcr primers and lcr probes for the amplifications and detection of human papilloma virus, and related kits and methods |
| US5484699A (en) * | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
| IT1244462B (it) | 1990-12-06 | 1994-07-15 | Sclavo Spa | Oligonucleotidi sintetici utili per la diagnosi di infezione da tipi diversi di virus del gruppo papilloma e loro utilizzazione |
| IL97226A0 (en) | 1991-02-13 | 1992-05-25 | Orgenics Ltd | Detection of high and low risk human papillomavirus by enzymatic amplification of dna |
| FR2679254B1 (fr) | 1991-07-17 | 1993-11-19 | Clonatec | Sondes oligonucleotidiques pour le typage et la detection des papillomavirus humains. |
| US5346811A (en) | 1991-07-22 | 1994-09-13 | Cerveceria Polar | Method and products for human papillomavirus detection |
| US5474796A (en) * | 1991-09-04 | 1995-12-12 | Protogene Laboratories, Inc. | Method and apparatus for conducting an array of chemical reactions on a support surface |
| ZA929351B (en) * | 1991-12-11 | 1993-06-04 | Igen Inc | Electrochemiluminescent label for DNA assays. |
| ZA929319B (en) * | 1991-12-11 | 1993-05-24 | Igen Inc | Method for exponential amplification of nucleic acid by a single unpaired primer. |
| WO1993022460A1 (en) * | 1992-05-06 | 1993-11-11 | Gen-Probe Incorporated | Nucleic acid amplification oligonucleotides and probes to human hepatitis b virus |
| US5428803A (en) | 1992-07-10 | 1995-06-27 | Cray Research, Inc. | Method and apparatus for a unified parallel processing architecture |
| JPH07508891A (ja) * | 1993-05-06 | 1995-10-05 | デイド、インターナショナル、インコーポレイテッド | ヒト乳頭種ウイルス検出アッセイ |
| SG63589A1 (en) | 1993-05-14 | 1999-03-30 | Johnson & Johnson Clin Diag | Diagnostic compositions elements methods and test kits for amplification and detection of two or more dna's using primers having matched melting temperatures |
| US6174668B1 (en) * | 1993-05-14 | 2001-01-16 | Johnson & Johnson Clinical Diagnostics, Inc. | Diagnostic compositions, elements, methods and test kits for amplification and detection of two or more target DNA's using primers having matched melting temperatures |
| US5679509A (en) * | 1993-09-28 | 1997-10-21 | University Of New Mexico | Methods and a diagnostic aid for distinguishing a subset of HPV that is associated with an increased risk of developing cervical dysplasia and cervical cancer |
| WO1995024499A1 (en) * | 1994-03-10 | 1995-09-14 | Gen-Probe Incorporated | Method for suppressing inhibition of enzyme-mediated reactions by ionic detergents |
| AU2584395A (en) * | 1994-04-26 | 1995-11-16 | Genta Incorporated | Antisense oligomers for inhibiting human papillomaviruses |
| JPH10504448A (ja) | 1994-06-15 | 1998-05-06 | ザ・トラスティーズ・オブ・コランビア・ユニバーシティー・イン・ザ・シティー・オブ・ニューヨーク | 腫瘍抑制遺伝子を同定する方法 |
| US5681702A (en) | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| DE4431174A1 (de) * | 1994-09-01 | 1996-03-07 | Deutsches Krebsforsch | Nachweisverfahren für tumorspezifische mRNA |
| JPH11508685A (ja) * | 1995-07-04 | 1999-07-27 | ドイチェス クレブスフォルシュンクスツェントルム スチフトゥング デス エッフェントリヒェン レヒツ | Ｈｐｖ蛋白質に対する特異的抗体の検出方法 |
| WO1997018334A2 (en) | 1995-11-15 | 1997-05-22 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| WO2006063065A2 (en) | 2004-12-08 | 2006-06-15 | Gen-Probe Incorporated | Detection of nucleic acids from multiple types of human papillomaviruses |
| WO2008138003A1 (en) | 2007-05-08 | 2008-11-13 | The Regents Of The University Of California | High-discharge-rate lithium ion battery |
-
1996
- 1996-11-12 WO PCT/US1996/018711 patent/WO1997018334A2/en not_active Ceased
- 1996-11-12 JP JP9519172A patent/JP2000500342A/ja not_active Ceased
- 1996-11-12 AU AU11621/97A patent/AU726047B2/en not_active Expired
- 1996-11-12 CA CA2237891A patent/CA2237891C/en not_active Expired - Lifetime
- 1996-11-14 US US08/749,955 patent/US6583278B1/en not_active Expired - Fee Related
- 1996-11-15 EP EP96308264A patent/EP0774518A3/en not_active Ceased
-
2003
- 2003-06-23 US US10/601,913 patent/US7470512B2/en not_active Expired - Fee Related
- 2003-06-26 US US10/607,416 patent/US7355034B2/en not_active Expired - Fee Related
-
2007
- 2007-10-30 US US11/929,753 patent/US7875441B2/en not_active Expired - Fee Related
-
2010
- 2010-12-10 US US12/965,728 patent/US8501410B2/en not_active Expired - Fee Related
-
2013
- 2013-06-21 US US13/924,087 patent/US9194008B2/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2004267215A (ja) * | 2000-08-03 | 2004-09-30 | National Institute Of Advanced Industrial & Technology | 核酸の測定方法、それに用いる核酸プローブ及びその方法によって得られるデータを解析する方法 |
| JP2004248678A (ja) * | 2000-09-26 | 2004-09-09 | National Institute Of Advanced Industrial & Technology | 新規核酸プローブ並びにそれを用いる核酸測定方法、及びその方法によって得られるデータを解析する方法 |
| JP2008524983A (ja) * | 2004-10-04 | 2008-07-17 | グッジェン インク | ヒトパピローマウイルスのプローブおよびそれを使用するｄｎａチップ |
| WO2007148762A1 (ja) * | 2006-06-22 | 2007-12-27 | Masahiko Kuroda | ヒトパピローマウイルス（ｈｐｖ）の検出方法、それに用いるプライマーセットおよび検出キット |
| WO2020050350A1 (ja) * | 2018-09-07 | 2020-03-12 | 株式会社ヘルスケアシステムズ | ヒトパピローマウイルスの検出方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| US7470512B2 (en) | 2008-12-30 |
| US9194008B2 (en) | 2015-11-24 |
| US20080311560A1 (en) | 2008-12-18 |
| CA2237891A1 (en) | 1997-05-22 |
| WO1997018334A3 (en) | 1997-10-02 |
| US7875441B2 (en) | 2011-01-25 |
| AU1162197A (en) | 1997-06-05 |
| CA2237891C (en) | 2013-07-30 |
| US6583278B1 (en) | 2003-06-24 |
| EP0774518A2 (en) | 1997-05-21 |
| US20050260562A1 (en) | 2005-11-24 |
| US7355034B2 (en) | 2008-04-08 |
| WO1997018334A2 (en) | 1997-05-22 |
| AU726047B2 (en) | 2000-10-26 |
| EP0774518A3 (en) | 2000-11-22 |
| US20110223584A1 (en) | 2011-09-15 |
| US20040018539A1 (en) | 2004-01-29 |
| US20150037785A1 (en) | 2015-02-05 |
| US8501410B2 (en) | 2013-08-06 |
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