JP2000350525A - Growth and rearing of taro seedling for setting - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a method for growing and rearing taro seedling for setting, capable of harvesting and feeding taro before October period which is real harvesting stage oppositely to stage at which taro begins to appear on the market from other warm area. SOLUTION: The meristem of taro is taken out in a germ-free state and the taro is subjected to shoot apex culture. As a result, many virus-free tubers of multiple shoots are reared and proliferated and tubers of these multiple shoots are taken out from a culturing chamber and divided by one by one and each one tuber is directly planted into soil by shallow planting every each cell of a cell tray 4 to which soil is packed and in a warm bed tunnel 8, water control and a prescribed temperature control by bottom water absorption are practiced for a prescribed period every cell tray 4 to promote rooting of tubers of taro and then, the taro is gradually acclimatized to the outside air to adapt the taro to setting condition and warm bed tunnel 8 is completely removed and the taro is reared to setting seedling while properly carrying out watering control.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The object of the present invention is to provide a pot in the Tohoku region, especially in the area of Yamagata Prefecture, especially in the early autumn. Add beef or pork to your favorite ingredients such as taro, konjac, leeks, carrots, etc. In a holiday event where you can enjoy nature at the same time, a large amount of freshly harvested taro, which is indispensable in soy sauce or miso, is harvested in the homeland of `` Imonikai '' where the harvest season is delayed due to climatic conditions Will also be able to produce and supply fresh local taro in time, and at the same time to meet the nationwide demand for taro as a taste of autumn by using other than the "Imonikai" In contrast to the timing of starting to go out from other temperate regions, we will be able to harvest and supply before the October season, which is the full-fledged harvesting period in the Tohoku region, where conventional cultivation methods will be delayed. It is an object of the present invention to provide a new method of growing and growing taro seedlings for planting, which enables cultivation farmers to make taro cultivation advantageous in terms of supply unit price.

[0002]

[Prior art] Agricultural products produced in the Tohoku region are:
It is not limited to specific crops such as fruits such as apples and cherries, such as mustard vegetables, green vegetables, safflower, etc. Not a few types are cultivated and harvested in temperate regions, and even if they are cultivated and harvested nationwide, most of them are produced from temperate regions. Not only is it not inferior in quality compared to other products, there are also many agricultural and horticultural products that boast higher quality, so even though it is a snowfall area, it is never a place of production of various agricultural and horticultural products. Although not inferior, due to its climatic conditions, the harvest season is generally delayed, and the time to go to the market is forced to fall behind, depending on the type of agricultural horticultural product. Because of its harvest time delay, it is also found that extremely difficult to benefited specific producers benefit high rate that would be obtained as the season.

[0003] One of them is "taro", which is a central ingredient that is indispensable in the "Imonikai", which is becoming famous throughout the country as one of Yamagata Prefecture's special events. Can be This taro is originally from tropical Asia such as India and China, is brought to Japan as the southern people move, and is one of the very old root vegetables that have been indigenous since ancient times. About 2 at most
It is said that there are as many as 00 varieties, but in Japan, varieties are broadly divided into "rice varieties", "parent potatoes and varieties used for potatoes", "parent varieties for potatoes", and "varieties for petioles". In the mainstream, in particular, as common foods, "variety for potatoes", for example, early Ishikawa, Todori, crow sowing, and those that draw the flow have been cultivated many.

[0004] The general method of cultivating and harvesting taro is to select seeds that are well preserved over the years and have a low consumption of about 40 to 50 grams, and are laid from mid-April to late April, and are difficult to dry in summer. The field is selected and ridged, seeds are planted at an interval of 25 to 40 cm (depending on the kind of seeds) in a planting groove of about 10 cm with the seeds facing upward, and the soil is covered relatively thinly of about 5 to 6 cm. When the buds began to emerge, a polyethylene mulch was used to raise the soil temperature. One month ago, excellent seeds are arranged in a frame, and the soil is 3 ~
After irrigating to a thickness of 5 cm, the entire frame is covered with a vinyl sheet to raise the temperature during the day, and germination work is performed, such as using a como at night, and then transplanted to a predetermined ridged field. After cultivation, cultivation management is carried out several times at the optimal time according to the growth, and careful management of water is performed to prevent abnormal drying. In the southwestern temperate region, early harvesting in mid-August, and direct planting, depending on the region, will reach the full harvest season in late October or mid-October. It depends on the way.

[0005] Therefore, according to this general direct planting cultivation, Yamagata boasts the highest consumption expenditure per household in Japan (Hokkaido Household Survey Annual Report, 1997) and is the main cause of consumption. I couldn't make it in September
Although it is only possible to make it from other prefectures and it is a mass consumption area, it has killed the willingness of producers in the prefecture to grow, and the planted area is only about 1% of the national market share. In order to break the situation, the company plans to expand the market share and adjust the harvest time, sprout and transplant cultivation is not instructed, but it manages the above-mentioned conventional seed potato as it is, The cultivation method to be transplanted has been quite successful so far, in addition to the climatic conditions of cold climates, as well as the problems of competition with other crop cultivation periods and the special circumstances of farmers in the prefecture suffering from labor shortages. Because there is no leverage, in the leading research and research institutions such as the Yamagata Prefectural Horticultural Experiment Station, which plays a part in agricultural administration,
Over the years, there has been a search for more effective measures to increase taro production in order to foster production farmers by taking advantage of the nation's best location for consumption.

[0006] It has been well known among related parties that sprouting and transplanting cultivation methods can be an important decisive factor as one of the effective cultivation methods for adjusting the taro harvest time and increasing yield. However, as long as the cultivation method relies on seed potatoes, as in the conventional direct planting cultivation method, problems of securing a large number of excellent seed potatoes and over-year management, securing work labor for sprouting management and cultivating other crops There are many difficulties and problems that are far from improving the production efficiency, such as problems of work adjustments between the plants. Tests and research on the possibility of producing robust and excellent taro planting seedlings were started.

[0007] In order to improve the cultivation effect by utilizing this shoot tip culture technology, it is necessary to develop a system capable of supplying a large amount of virus-free seedlings in a short period of time.
While adopting nutrient cultivation as a system for that,
Production is limited to bio-factories that can be controlled intensively, such as complete control of light, temperature, and humidity. Because of the considerable difficulty in terms of point, even with the use of shoot apex culture, various trial and error and repeated trial production experiments have been repeated, based on the judgment that simpler techniques for making planted seedlings are not feasible. As a result, we focused on the seed-specific properties of taro, namely its rooting power and high water resistance (the ability of tubers to survive without rot under flooded conditions), and efficient stems utilizing these properties The possibility of top culture has emerged.

That is, in the case of a general plant, it is necessary to strictly manage the rooting using a rooting medium in a culture bottle, and then take out the same and gradually adjust it to an environment such as temperature, light and humidity. In contrast to this, management cultivation was inevitably performed in a well-developed biofactory, but in the present invention, rooting management in a culture bottle was achieved by taking advantage of the characteristics of "taro". It was confirmed for the first time that rooting and acclimatization could be easily performed without
Even without the need for a large-scale biofactory as before, the clonal seedling of "Taro" is genetically uniform and virus-free by vegetative propagation, in which the shoot apex is grown to grow foliage and roots. Has succeeded in realizing a new method of growing and growing taro seedlings for permanent planting for seedling production technology that enables efficient mass production of taro.The details of the composition will be described in order below. To

[0009]

The gist of the method for growing and growing taro seedlings for planting according to the present invention is as follows. That is, the taro growth points are aseptically removed and cultivated in the shoot apex to grow and multiply a large number of virus-free polysprouts from one growth point, and then divide the individual multibuds into tubers. Each of the tubers was directly planted as a shallow plant in soil in a container for storing water that can absorb water from the bottom in an environment free from aseptic culture, and planted in a facility capable of controlling temperature and light quantity. For each plant, water management, temperature management and light amount management by bottom water supply are carried out for a predetermined period to promote rooting from taro tubers, and to gradually grow into constant seedlings while gradually acclimating to outside air. This is a method of growing and growing taro seedlings.

In the method for growing and growing taro seedlings for planting according to the present invention having the above-mentioned basic constitution, the taro growing points are isolated under aseptic conditions and cultured at the shoot apex to remove viruses from one growing point. After growing and growing a large number of shoots, each individual shoot is divided for each tuber, and each of them is
In a facility where the cell tray filled with soil in an environment free from aseptic culture conditions is planted directly in the soil as a shallow planting form, and in a facility where temperature and light quantity can be controlled, the water content of each planted cell tray and bottom surface water supply A taro seedling for planting with a configuration that promotes rooting from a taro tuber by performing management, temperature control, and light amount control for a predetermined period, and gradually grows to a planting seedling while gradually acclimating to outside air. And the method of growing and growing.

Similarly, the taro growing point is aseptically removed and cultivated in the shoot apex to grow and proliferate a large number of virus-free polyblasts from one growing point. Each of them, and each of them is directly planted in the soil as a shallow planting form for each cell of the cell tray filled with the soil under the environment released from the aseptic culture,
In the hotbed tunnel, for each planted cell tray, water management, temperature management and light quantity management by bottom water supply are carried out for a predetermined period to promote rooting from taro tubers, and after finishing management to gradually acclimate to outside air Therefore, the method also encompasses a method of growing and growing taro seedlings for fixed planting, in which the hotbed tunnel is completely removed, and the seedlings are grown to fixed planting seedlings while appropriately managing irrigation.

If the method of growing and growing taro seedlings for planting having the basic structure is described as a more specific structure, it can be said that the gist is a structure comprising the following five steps. First, the taro growing point was aseptically removed and cultivated in the shoot apex to obtain a virus-free multi-bud, and the tubers of the multi-bud were divided into individual tubers and then sterile-cultured again. A step of further growing and growing a large number of multiple shoots from one growth point by further growing the split and raised multiple shoots.

The shoots obtained from the above step are divided for each tuber, and each of them is placed in each cell of a cell tray filled with soil, which is placed in an environment freed from aseptic culture. The process of planting the growing point directly in the soil as a shallow planting shape that will not be buried.

Each of the planted cell trays is set in a water supply container capable of supplying water to the bottom surface, and is placed in a closed hotbed tunnel for a predetermined period under the control of temperature, light quantity, moisture and the like. The process of promoting rooting.

Thereafter, the sealed state of the hot-bed tunnel is gradually released, and the foliage is gradually adapted to the outside air while maintaining the temperature control at about 20 to 25 ° C., the light quantity control, and the water supply control over a predetermined period. .

In this step, the hotbed tunnel is completely removed, and the seedlings are cultivated to constant planting while appropriately managing irrigation.

If the method of growing and growing taro seedlings for planting consisting of these five steps is shown as a more specific structure, the taro growing points are aseptically extracted, shoot-top-cultured, and virus-free. After obtaining the buds, the tubers of the buds are divided into individual pieces, and then germ-free cultivation is performed again. A process of growing and multiplying a large number.

The multiple shoots obtained from the above step are divided into tubers, and each of them is placed in each cell of a cell tray filled with soil, which is placed in an environment free from aseptic culture. The process of planting the growing point directly in the soil as a shallow planting shape that will not be buried. Each planted cell tray is set in a water supply container that allows water to be supplied to the bottom surface, and then installed in a sealed hotbed tunnel prepared in a pipe house, under temperature control of about 25 to 30 ° C, light quantity control, and water supply control. Growing for about 2 weeks to promote rooting from taro tubers

Thereafter, the sealed state of the hot-bed tunnel is gradually released, and the foliage is gradually adapted to the outside air while maintaining the temperature control at about 20 to 25 ° C., the light quantity control, and the water supply control over a predetermined period. . Remove the hotbed tunnel completely,
This is a method of growing and growing taro seedlings for fixed planting, which comprises the above five steps of growing the seeds to fixed planting seedlings while appropriately managing irrigation.

In the shoot apex culture, the shoots of taro are cut off, disinfected, and then dissected under a stereoscopic microscope on a sterile worktable.
Growth point is about 0.2-0.5mm, preferably 0.3m
m, in a conical beaker or a primary medium in a culture bottle, for example, LS medium (3% sucrose, pH5.
8) Put on the top and culture the shoot apex under aseptic condition,
By this aseptic cultivation, virus-free multibud tubers are grown to tubers, and the multibuds are further divided into tubers one by one, and then placed in a conical beaker or a growth medium in a culture bottle. Then, the culture is subjected to aseptic culture by shaking culture or static culture, and over a predetermined period of time, is grown into a large number of polyblasts, thereby multiplying 5 to 7, and in some cases, 7 or 8 tubers. By growing the tuber and further dividing the tuber into tubers, a large number of genetically uniform cloned tubers are grown and propagated from one growth point. During this time, the shoot apex culture technique is basically the same as the conventional means.

The tubers of the virus-free taro multi-buds multiplied from one growth point in this way are all removed from the conical beaker or the culture bottle, and after the aseptic growth culture of FIG. As shown in the process diagram showing the process of dividing and placing the plant, the tuber is divided into tubers, and the leaves and roots are desirably removed, and the tuber (potato) portion and the petiole are formed. Each of them is placed in an environment free from aseptic culture, filled with soil, and placed in a storage container with a structure capable of absorbing water on the bottom, and is preferably partitioned so that each tuber can be planted. The cell tray is filled with the medium, and the growing point is directly planted in the medium as a shallow planting shape that does not bury the growing point. After that, the planted container, for example, for each cell tray, is set in a water supply container capable of supplying water to the bottom surface, and the entire container for the floor is provided with a facility capable of controlling the temperature and light amount, for example, a sterile room. If there is room in the bio factory, it can be inside the bio factory, but if not, a general greenhouse such as a pipe house is prepared separately and installed in a simple facility such as a closed hotbed tunnel inside it In addition, root management from taro tubers is promoted by continuing temperature control and water supply control at about 25 to 30 ° C. for about two weeks as a whole. This rooting promotion step can be realized for the first time due to the characteristics of taro having high rooting power and high water resistance.

In order to make this rooting promotion process more reliable, it does not mean that there is no influence on the efficiency of the practical application stage, but it is necessary to make the rooting promotion process about one week immediately after installation in the hotbed tunnel. Covered with wrap over the period, so as to minimize the drying of the initial stage of each tuber planted for each cell, and then gradually remove the wrap,
If the management is carried out, the promotion of rooting becomes more certain, which is extremely advantageous.

As described above, the step of promoting rooting of the tubers of the virus-free taro multi-bud is divided for each tuber, and is released from a sterile culture state, which has not been performed before. It is planted in the manner of "cutting" directly on the soil underneath, and in a sealed hotbed tunnel, 25
After continuing the temperature management of -30 ° C, light quantity management and water supply management for about 2 weeks, the sealed state of the hotbed tunnel is gradually released for about 2 weeks. Therefore, the habitat should be adapted to the environment of about 20 to 25 ° C.
Temperature management of about 20 ° C, light quantity management and water supply management should be continued for about one month, and over about two months, the foliage of taro tubers should be completely acclimated to the open air to satisfy the planting conditions. Thus, the steps for the method for growing and growing a new taro seedling for permanent planting of the present invention are completed.

Therefore, unlike the conventional multiplication cultivation method using the shoot apex culture, the management of rooting promotion and acclimation is not limited to a limited space such as a bio factory, and temperature and light amount can be controlled. Since it can be carried out even in a simple facility such as a pipe house, it is possible to mass-produce the proliferating seedling itself, greatly reduce the economic burden for improving production efficiency, and adjust the harvest time and the accompanying advantageous production price. Thus, a method for growing and growing taro seedlings for planting can be realized, which is extremely advantageous in increasing farmers' willingness to produce taro. Hereinafter, representative examples will be described in detail so that the above-described method for growing and growing taro seedlings for planting according to the present invention can be more clearly understood.

[0025]

FIG. 1 is a schematic cross-sectional view for explaining the cultivation state in the process of promoting rooting and acclimatization, FIG. 2 is a process diagram for explaining a process of cutting and disinfecting a taro bud, and FIG. Is a schematic whole perspective view showing the cutting of the growing point and the primary aseptic culture, FIG. 4 is a process diagram showing a process of dividing from multiple shoots into tubers and placing them after the aseptic growth culture process, Temperature control,
As a facility capable of controlling the amount of light, the method of growing and growing a representative taro seedling for regular planting according to the present invention, which is implemented using a hotbed tunnel in a simple pipe house instead of a bio factory as in the past, will be specifically described. To do
This embodiment is as follows.

A potato with low consumption is selected, divided into about 20 g or a little larger, and immersed in a bleached powder solution diluted 300 times for about 1 minute, and then Benrate T wettable powder (trade name) 20 times Soak in the solution, after about 15 minutes, take out and shade, put the seed potatoes in a connection pot, fill with commercially available soil, and take 7-10 days at a temperature of about 25 ° C It is used as a taro for sprouting and cutting out the shoot apex.

As shown in the process diagram of FIG. 2, the germinated taro T is first thoroughly washed with a neutral detergent D by rubbing the entire surface thereof by hand as a preparatory operation before cutting off the shoot apex. After dropping, wash off the neutral detergent D with enough water and drain well in a Petri dish covered with two layers of filter paper. Then, the sprouted taro T is spread on aluminum foil and held firmly with tweezers on the filter paper. With a scalpel using a double-edged razor, the bud tip is about 2c below its base
The potato portion T1 is cut out so as to have a potato portion of about m, and the tip portion T1 is immersed in a 1% sodium hypochlorite solution N for about 15 minutes for sterilization and disinfection. ,
After the operation of washing with sterile water is repeated about three times, the operation of draining is completed, and preparation is made for the next cutting off of the shoot apex.

The sprout portion T1, which has been completely sterilized, is then dissected at the bud tip under a stereoscopic microscope on a sterile worktable.
A growth point (stem tip) 1 of about 3 mm is cut out, and as shown in a schematic whole perspective view showing the primary aseptic culture in FIG. 3, a primary culture medium K in a culture bottle 2, for example, an LS medium (3% sucrose,
pH 5.8) and the hormone condition was NAA 0.1 mg / L x BA 0.
Carefully transfer above 02mg / l at 25 ° C, 1
After aseptic cultivation for about 40 days with a photoperiod of 6 hours (6,000 Lux), the young leaves are grown to about 5 cm, and are grown into virus-free tubers.

The virus-free tubers grown by primary aseptic culture are further placed on a growth medium K1 in a conical beaker or culture bottle 2 for each tuber.
Aseptic cultivation is continued again for about 1 to 2 months so that the virus-free polyblasts 3 each having 5 to 7 tubers 31, 31,... The growth culture at this stage can be carried out by either shaking culture in a liquid medium or static culture in a solid medium.

In the shaking culture using the liquid medium, the basic medium is, for example, an LS medium (sucrose 3%, pH5.
8) A medium having a hormonal condition of 0.25 mg BA per liter was placed in a 100 ml conical beaker.
About 0 milliliter is injected, and about 1 month is applied for aseptic culturing while shaking at 100 rpm. In still culture using a solid medium, for example, LS is used as a basic medium.
Medium (3% sucrose, pH 5.8), support is agar 0.8
% Or gerritite 0.15% BA
In a culture bottle filled with 0.5 mg / liter medium, about 2 months, 25 ° C., 16 hours photoperiod (6,000 hours)
By sterile culturing under the condition of 0 Lux), virus-free polyblasts having 5 to 7 tubers necessary for the next step can be obtained. In addition, various instruments such as petri dishes, filter paper, aluminum foil, scalpels for cutting out shoot tips, culture bottles, conical beakers, etc., which are necessary for aseptic culture in the previous steps, must be used after being strictly sterilized and disinfected. The same applies to petri dishes and filter paper used in the sprouting operation.

Thus, the polyblasts 3, 3,..., Which are grown from 5 times to 14 to 16 times as large as the tuber through the primary culture, the primary culture, and the subsequent multiplication culture, and become virus-free, Removed from the culture bottle or conical beaker up to that time, each one tuber 3
1, 31, .... At that time, each tuber 31,3
1, remove roots and leaves that grow from “Potato + Petiole”
When handled in a state, the rooting and acclimatization steps of the virus-free tuber 31, which is one of the characteristic constitutions of the present invention, in the soil can be further assured, which is extremely convenient. It will be.

Each of the virus-free tubers 31, 31,..., Which has been divided into a “potato + stalk” shape, is filled with soil 5 for root acclimation in a cell tray 4 prepared in advance as a container for placing on a floor. The plant is planted for each of the cells 41. The planting is performed by making a hole in the root acclimating soil 5 in the cell 41, and shallow planting such that the growing point is not hidden, that is, in the manner of “insert bud”, and then lightly press the planting hole. . Virus-free tuber 31, in all cells 41, 41, ...
The cell tray 4 in which each one of 31,... Has been planted, as shown in a schematic cross-sectional view for explaining the cultivation state in the rooting promotion and acclimatization process of FIG. The cell tray 4 is set in a water supply container 6 into which water has been injected 7 to a predetermined depth so that water can be absorbed from the bottom surface of the cell tray 4.
The root acclimatization soil 5 is Metromix 35.
0 (trade name) and vermiculite in a ratio of 1: 1 and mixed with Micro Long Total 10 as fertilizer.
A product obtained by adding a 0-day type (trade name) at a rate of 1.5 g / liter is used.

The cell tray 4 set in the water supply container 6
Preferably, for about one week in the initial stage, it is installed in a sealed hotbed tunnel 8 provided in a pipe house, for example, by covering it with wrap to surely prevent drying immediately after placing on the floor,
While keeping the temperature at 25 to 30 ° C., the light amount was adjusted by covering with non-woven fabric 9, sufficient moisture management was performed, and the wrap was gradually removed around one week after the lapse of about one week. Each virus-free tuber 31,3 in the period of about
Promotes rooting from 1, ....

After 2 weeks of the rooting promotion step,
While gradually removing the non-woven fabric 9, the sealed state of the hotbed tunnel 8 is gradually opened over a period of about two weeks, during which the temperature control at 20 to 25 ° C. and the case of the cell tray 4 are performed. In order to facilitate drying, sufficient water supply management is performed. After about one month, the hotbed tunnel 8 is completely removed and exposed to the outside air, and further about 15 months in the pipe house for about 1 month. By adjusting to the outside environment while maintaining the temperature range of ~ 20 ° C,
After the stems and leaves of the taro tuber 31 are gradually acclimated to the open air, irrigation management is also appropriately performed in the finishing stage to adjust to the planting conditions, and the virus-free tubers 31, 31,... In two months, the plant will eventually grow into a large number of excellent seedlings.

[0035]

[Effects] The method of growing and growing taro seedlings for planting according to the present invention, which is carried out by the above-described steps, is carried out from early to late September of the taro harvesting season. Examining the quality (tasting) and other factors, selecting seeds of superior strains, and extracting the shoot apex from the selected seeds at the optimal time calculated backward from the end of April to the beginning of May according to the production of fixed seedlings, Perform a primary aseptic culture by placing the bed,
Subsequently, after the growth of aseptic culture, 4
During about two months to the end of the month, the virus-free multibud was divided into tubers, and the tubers were directly inserted into the soil in an environment free of aseptic culture in the manner of “insert buds”. Planting shallowly to promote rooting, and then gradually acclimated to the external environment to grow into excellent fixed planting seedlings, and from the end of April to the beginning of May, set up ridges in the field, set up cultivation management and pest control In addition, since the drying in the July-August season of the fattening season has a great effect on the harvest, appropriate furrow irrigation is carried out at this time, so that during the early and mid-September, the early early digging taro harvesting season Taro can be reliably harvested at the time of losing.

The taro seedling for planting produced by the method of growing and growing according to the present invention is a compact seedling having no seed potato portion.
In particular, those produced by using a cell tray as a container for placing are difficult to remove soil and hair roots around the seedlings and are extremely excellent as fixed planting seedlings. Although the plant height is rather small in comparison, the potatoes grow faster and have a strong tendency to enlarge,
It is very suitable for early digging and high harvesting. Yamagata shows that the test cultivation results show that the yield is 1.8 times larger than that of conventional seed potato seedlings, and the ratio of large balls is considerably higher. It has been proved by the test results of the prefectural horticultural testing station.

The results of this test show that, in the case of a conventional seed potato seedling, the growth of the foliage takes precedence as the vegetative growth.
In the case of the virus-free planting method of the present invention, a large number of Coulomb seedlings are produced from the selected excellent seeds, while the number of the seedlings is delayed and the number does not increase so much. Coulomb seedlings are all of excellent quality,
Because there is no potato (nutrition) part, plant instinct to leave offspring, prioritize the way to make potatoes before nutrients from the roots are passed on to the growth of foliage, as a result, The yield increases,
Conversely, it is expected that the plant height will be inferior to that of conventional seed potato seedlings.

The method of growing and growing taro seedlings for planting according to the present invention differs from the conventional root acclimation process in that many tubers, which have been virus-free by shoot apex culture, are released from the aseptic culture environment. It is a plant that is directly shallowly planted in the rooting acclimation soil below, and can adopt an innovative root acclimation process that controls water supply, prescribed temperature control, and light intensity. It is not necessarily limited to the limited facility environment inside, so it is also possible to produce planted seedlings in a simple facility such as a pipe house. It is not necessary to prepare suitable facilities, and the great advantage that the economic burden to increase production efficiency can be greatly reduced is obtained, and it is extremely easy to provide planted seedlings at the harvest time. Thereby achieving the Xiu features.

As a result, for example, as a new taro digging in early September, which coincides with the beginning of the “Imoni-kai” event in Yamagata Prefecture, which is a large consumption area of taro, it is possible to make adjustment shipments from local farmers. It is no longer necessary to rely on the early pick-up of new taro from the region, and the meaning of the hometown event is strengthened as a `` potato stewed by local taro '', which contributes to the promotion of tourism and contributes to the promotion of the region, and above all, As a result, it is possible to secure a favorable production price as a fast-digging new taro, stimulate the willingness of local farmers to produce, stabilize farm management as a new production item, and greatly contribute to regional revitalization. You can expect it.

In particular, according to the method of growing and growing taro seedlings for planting, which is representative of the present invention described in the embodiments, high-quality planting can be performed by a simple facility such as a pipe house without preparing special facilities. It is possible to ensure the large-scale cultivation and propagation of seedlings, not only for traditional taro producers, but also for producers who are going to newly enter, without excessive burden on initial investment. It will be possible to work on attractive taro production that can be dug and harvested, and that the increase in yield will be almost guaranteed, thereby increasing the number of taro producers and exerting its power in stabilizing the supply system of high quality taro .

As described above, the method for growing and growing taro seedlings for planting according to the present invention is based on an extremely novel structure that focuses on the unique plant characteristics of taro, and enables the intended purpose to be achieved universally. Yes, liberalization of agricultural products, change of agricultural administration, etc.
This technology is extremely effective in stabilizing the management base of farmers facing a severe environment due to various factors, and has been highly evaluated by many farmers under the guidance of the responsible administrative organization and related research and research institutes, and taro production It is expected to greatly contribute to the spread and expansion of.

[Brief description of the drawings]

The drawings are for describing a typical embodiment of the method for growing and growing taro seedlings for planting of the present invention.

FIG. 1 is a schematic cross-sectional view for explaining a cultivation state in a rooting promotion and acclimatization process.

FIG. 2 is a process diagram for explaining a process of cutting and disinfecting a taro bud tip.

FIG. 3 is a schematic whole perspective view showing cutting of a growing point and primary aseptic culture.

FIG. 4 is a process chart showing a process of dividing from multiple buds into tubers and placing them on a bed after a sterile growth culture process.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Growth point (Stem tip) 2 Culture bottle 3 Virus-free polyblast 31 Tuber 4 Same as above 4 Container for bed (cell tray) 41 Same cell 5 Soil for root acclimation 6 Water supply container 6 7 Water injection 8 Hotbed tunnel 9 Nonwoven fabric D Medium Detergent N 1% sodium hypochlorite solution T Taro T1 Same bud tip

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[Procedure amendment]

[Submission date] June 16, 1999 (1999.6.1
6)

[Procedure amendment 2]

[Document name to be amended] Statement

[Correction target item name] Full text

[Correction method] Change

[Correction contents]

[Document Name] Statement

[Title of the Invention] Method of growing and growing taro seedlings for planting

[Claims]

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The object of the present invention is to provide a pot in the Tohoku region, especially in the area of Yamagata Prefecture, especially in the early autumn. Add beef or pork to your favorite ingredients such as taro, konjac, leeks, carrots, etc. In a holiday event where you can enjoy nature at the same time, a large amount of freshly harvested taro, which is indispensable in soy sauce or miso, is harvested in the homeland of `` Imonikai '' where the harvest season is delayed due to climatic conditions Will also be able to produce and supply fresh local taro in time to meet the national demand for taro as a taste of autumn by using other than the “Imoni-kai”. However, in response to the time when it starts to go out from other temperate regions, it is possible to harvest and supply before the October season, which is the full-fledged harvesting season in the Tohoku region, where conventional cultivation methods are delayed. It is an object of the present invention to provide a new method of growing and growing taro seedlings for planting so that cultivation farmers in the region can make taro cultivation advantageous in terms of supply unit price.

[0002]

[Prior art] Agricultural products produced in the Tohoku region are:
It is not limited to specific crops such as fruits such as apples and cherries, such as mustard vegetables, green vegetables, safflower, etc. Not a few types are cultivated and harvested in temperate regions, and even if they are cultivated and harvested nationwide, most of them are produced from temperate regions. Not only is it not inferior in quality compared to other products, there are also many agricultural and horticultural products that boast higher quality, so even though it is a snowfall area, it is never a place of production of various agricultural and horticultural products. Although not inferior, due to its climatic conditions, the harvest season is generally delayed, and the time to go to the market is forced to fall behind, depending on the type of agricultural horticultural product. Because of its harvest time delay, it is also found that extremely difficult to benefited specific producers benefit high rate that would be obtained as the season.

[0003] One of them is "taro", which is a central ingredient that is indispensable in the "Imonikai", which is becoming famous throughout the country as one of Yamagata Prefecture's special events. Can be This taro is originally from tropical Asia such as India and China, is brought to Japan as the southern people move, and is one of the very old root vegetables that have been indigenous since ancient times. About 2 at most
It is said that there are as many as 00 varieties, but in Japan, varieties are broadly divided into "rice varieties", "parent potatoes and varieties used for potatoes", "parent varieties for potatoes", and "varieties for petioles". In the mainstream, in particular, as common foods, "variety for potatoes", for example, early Ishikawa, Todori, crow sowing, and those that draw the flow have been cultivated many.

[0004] The general method of cultivating and harvesting taro is to select seeds that are well preserved over the years and have a low consumption of about 40 to 50 grams, and are laid from mid-April to late April, and are difficult to dry in summer. The field is selected and ridged, seeds are planted at an interval of 25 to 40 cm (depending on the kind of seeds) in a planting groove of about 10 cm with the seeds facing upward, and the soil is covered relatively thinly of about 5 to 6 cm. When the buds began to emerge, a polyethylene mulch was used to raise the soil temperature. One month ago, excellent seeds are arranged in a frame, and the soil is 3 ~
After irrigating to a thickness of 5 cm, the entire frame is covered with a vinyl sheet to raise the temperature during the day, and germination work is performed, such as using a como at night, and then transplanted to a predetermined ridged field. After cultivation, cultivation management is carried out several times at the optimal time according to the growth, and careful management of water is performed to prevent abnormal drying. In the southwestern temperate region, early harvesting in mid-August, and direct planting, depending on the region, will reach the full harvest season in late October or mid-October. It depends on the way.

[0005] Therefore, according to this general direct planting cultivation, Yamagata boasts the highest consumption expenditure per household in Japan (Hokkaido Household Survey Annual Report, 1997) and is the main cause of consumption. I couldn't make it in September
Although it is only possible to make it from other prefectures and it is a mass consumption area, it has killed the willingness of producers in the prefecture to grow, and the planted area is only about 1% of the national market share. In order to break the situation, the company plans to expand the market share and adjust the harvest time, sprout and transplant cultivation is not instructed, but it manages the above-mentioned conventional seed potato as it is, The cultivation method to be transplanted has been quite successful so far, in addition to the climatic conditions of cold climates, as well as the problems of competition with other crop cultivation periods and the special circumstances of farmers in the prefecture suffering from labor shortages. Because there is no leverage, in the leading research and research institutions such as the Yamagata Prefectural Horticultural Experiment Station, which plays a part in agricultural administration,
Over the years, there has been a search for more effective measures to increase taro production in order to foster production farmers by taking advantage of the nation's best location for consumption.

[0006] It has been well known among related parties that sprouting and transplanting cultivation methods can be an important decisive factor as one of the effective cultivation methods for adjusting the taro harvest time and increasing yield. However, as long as the cultivation method relies on seed potatoes, as in the conventional direct planting cultivation method, problems of securing a large number of excellent seed potatoes and over-year management, securing work labor for sprouting management and cultivating other crops There are many difficulties and problems that are far from improving the production efficiency, such as problems of work adjustments between the plants. Tests and research on the possibility of producing robust and excellent taro planting seedlings were started.

[0007] In order to improve the cultivation effect by utilizing this shoot tip culture technology, it is necessary to develop a system capable of supplying a large amount of virus-free seedlings in a short period of time.
While adopting nutrient cultivation as a system for that,
Production is limited to bio-factories that can be controlled intensively, such as complete control of light, temperature, and humidity. Because of the considerable difficulty in terms of point, even with the use of shoot apex culture, various trial and error and repeated trial production experiments have been repeated, based on the judgment that simpler techniques for making planted seedlings are not feasible. As a result, we focused on the seed-specific properties of taro, namely its rooting power and high water resistance (the ability of tubers to survive without rot under flooded conditions), and efficient stems utilizing these properties The possibility of top culture has emerged.

That is, in the case of a general plant, it is necessary to strictly manage the rooting using a rooting medium in a culture bottle, and then take out the same and gradually adjust it to an environment such as temperature, light and humidity. In contrast to this, management cultivation was inevitably performed in a well-developed biofactory, but in the present invention, rooting management in a culture bottle was achieved by taking advantage of the characteristics of "taro". It was confirmed for the first time that rooting and acclimatization could be easily performed without
Even without the need for a large-scale biofactory as before, the clonal seedling of "Taro" is genetically uniform and virus-free by vegetative propagation, in which the shoot apex is grown to grow foliage and roots. Has succeeded in realizing a new method of growing and growing taro seedlings for permanent planting for seedling production technology that enables efficient mass production of taro.The details of the composition will be described in order below. To

[0009]

The gist of the method for growing and growing taro seedlings for planting according to the present invention is as follows. That is, the taro growth points are aseptically removed and cultivated in the shoot apex to grow and multiply a large number of virus-free polysprouts from one growth point, and then divide the individual multibuds into tubers. Each of the tubers was directly planted as a shallow plant in soil in a container for storing water that can absorb water from the bottom in an environment free from aseptic culture, and planted in a facility capable of controlling temperature and light quantity. For each plant, water management, temperature management and light amount management by bottom water supply are carried out for a predetermined period to promote rooting from taro tubers, and to gradually grow into constant seedlings while gradually acclimating to outside air. This is a method of growing and growing taro seedlings.

In the method for growing and growing taro seedlings for planting according to the present invention having the above-mentioned basic constitution, the taro growing points are isolated under aseptic conditions and cultured at the shoot apex to remove viruses from one growing point. After growing and growing a large number of shoots, each individual shoot is divided for each tuber, and each of them is
In a facility where the cell tray filled with soil in an environment free from aseptic culture conditions is planted directly in the soil as a shallow planting form, and in a facility where temperature and light quantity can be controlled, the water content of each planted cell tray and bottom surface water supply A taro seedling for planting with a configuration that promotes rooting from a taro tuber by performing management, temperature control, and light amount control for a predetermined period, and gradually grows to a planting seedling while gradually acclimating to outside air. And the method of growing and growing.

Similarly, the taro growing point is aseptically removed and cultivated in the shoot apex to grow and proliferate a large number of virus-free polyblasts from one growing point. Each of them, and each of them is directly planted in the soil as a shallow planting form for each cell of the cell tray filled with the soil under the environment released from the aseptic culture,
In the hotbed tunnel, for each planted cell tray, water management, temperature management and light quantity management by bottom water supply are carried out for a predetermined period to promote rooting from taro tubers, and after finishing management to gradually acclimate to outside air Therefore, the method also encompasses a method of growing and growing taro seedlings for fixed planting, in which the hotbed tunnel is completely removed, and the seedlings are grown to fixed planting seedlings while appropriately managing irrigation.

If the method of growing and growing taro seedlings for planting having the basic structure is described as a more specific structure, it can be said that the gist is a structure comprising the following five steps. First, the taro growing point was aseptically removed and cultivated in the shoot apex to obtain a virus-free multi-bud, and the tubers of the multi-bud were divided into individual tubers and then sterile-cultured again. A step of further growing and growing a large number of multiple shoots from one growth point by further growing the split and raised multiple shoots.

The shoots obtained from the above step are divided for each tuber, and each of them is placed in each cell of a cell tray filled with soil, which is placed in an environment freed from aseptic culture. The process of planting the growing point directly in the soil as a shallow planting shape that will not be buried.

Each of the planted cell trays is set in a water supply container capable of supplying water to the bottom surface, and is placed in a closed hotbed tunnel for a predetermined period under the control of temperature, light quantity, moisture and the like. The process of promoting rooting.

Thereafter, the sealed state of the hot-bed tunnel is gradually released, and the foliage is gradually adapted to the outside air while maintaining the temperature control at about 20 to 25 ° C., the light quantity control, and the water supply control over a predetermined period. .

In this step, the hotbed tunnel is completely removed, and the seedlings are cultivated to constant planting while appropriately managing irrigation.

If the method of growing and growing taro seedlings for planting consisting of these five steps is shown as a more specific structure, the taro growing points are aseptically extracted, shoot-top-cultured, and virus-free. After obtaining the buds, the tubers of the buds are divided into individual pieces, and then germ-free cultivation is performed again. A process of growing and multiplying a large number.

The multiple shoots obtained from the above step are divided into tubers, and each of them is placed in each cell of a cell tray filled with soil, which is placed in an environment free from aseptic culture. The process of planting the growing point directly in the soil as a shallow planting shape that will not be buried. Each planted cell tray is set in a water supply container that allows water to be supplied to the bottom surface, and then installed in a sealed hotbed tunnel prepared in a pipe house, under temperature control of about 25 to 30 ° C, light quantity control, and water supply control. A process of growing for about 2 weeks to promote rooting from taro tubers.

Thereafter, the sealed state of the hot-bed tunnel is gradually released, and the foliage is gradually adapted to the outside air while maintaining the temperature control at about 20 to 25 ° C., the light quantity control, and the water supply control over a predetermined period. . Remove the hotbed tunnel completely,
This is a method of growing and growing taro seedlings for fixed planting, which comprises the above five steps of growing the seeds to fixed planting seedlings while appropriately managing irrigation.

In the shoot apex culture, the shoots of taro are cut off, disinfected, and then dissected under a stereoscopic microscope on a sterile worktable.
Growth point is about 0.2-0.5mm, preferably 0.3m
m, and cut into a conical beaker or a primary culture medium in a culture bottle, for example, LS medium (3% sucrose, pH5.
8) Put on the top and culture the shoot apex under aseptic condition,
By this aseptic culture, virus-free multibud tubers were grown to tubers, and the multibuds were further divided into tubers one by one, and then placed in a conical beaker or a growth medium in a culture bottle. By performing aseptic cultivation by shaking culture or static culture and growing it into a large number of multibuds over a predetermined period of time, 5-7, and sometimes 7,8 multi-buds can be obtained. By growing the tuber and dividing it into tubers, a large number of genetically uniform cloned tubers are grown and proliferated from one growing point. During this time, the shoot apex culture technique is basically the same as the conventional means.

The tubers of the virus-free taro multi-buds multiplied from one growth point in this way are all removed from the conical beaker or the culture bottle, and after the aseptic growth culture of FIG. As shown in the process diagram showing the process of dividing and placing the plant, the tuber is divided into tubers, and the leaves and roots are desirably removed, and the tuber (potato) portion and the petiole are formed. Each of them is placed in an environment free from aseptic culture, filled with soil, and is divided so as to be able to be implanted per tuber, preferably with a bottom-absorbing structure. In the case of cell trays that are filled with soil in the cell tray, the growth points are planted directly in the soil as a shallow planting shape that does not bury the growth point, that is, planting in a manner that `` cuts and buds '' After that, the planted storage containers, for example, each cell tray, are set in a water supply container capable of supplying water to the bottom surface, and the entire storage container is provided with a facility capable of controlling the temperature and light amount, such as a sterile room. If there is room in the bio factory, it can be inside the bio factory, but if not, a general greenhouse such as a pipe house is prepared separately and installed in a simple facility such as a closed hotbed tunnel inside it In addition, root management from taro tubers is promoted by continuing temperature control and water supply control at about 25 to 30 ° C. for about two weeks as a whole. This rooting promotion step can be realized for the first time due to the characteristics of taro having high rooting power and high water resistance.

In order to make this rooting promotion process more reliable, it does not mean that there is no influence on the efficiency of the practical application stage, but it is necessary to make the rooting promotion process about one week immediately after installation in the hotbed tunnel. Covered with wrap over the period, so as to minimize the drying of the initial stage of each tuber planted for each cell, and then gradually remove the wrap,
If the management is carried out, the promotion of rooting becomes more certain, which is extremely advantageous.

As described above, the step of promoting rooting of the tubers of the virus-free taro multi-bud is divided for each tuber, and is released from a sterile culture state, which has not been performed before. It is planted in the manner of "cutting" directly on the soil underneath, and in a sealed hotbed tunnel, 25
After continuing the temperature management of -30 ° C, light quantity management and water supply management for about 2 weeks, the sealed state of the hotbed tunnel is gradually released for about 2 weeks. Therefore, the habitat should be adapted to the environment of about 20 to 25 ° C.
Temperature management of about 20 ° C, light quantity management and water supply management should be continued for about one month, and over about two months, the foliage of taro tubers should be completely acclimated to the open air to satisfy the planting conditions. Thus, the steps for the method for growing and growing a new taro seedling for permanent planting of the present invention are completed.

Therefore, unlike the conventional multiplication cultivation method using the shoot apex culture, the management of rooting promotion and acclimation is not limited to a limited space such as a bio factory, and temperature and light amount can be controlled. Since it can be carried out even in a simple facility such as a pipe house, it is possible to mass-produce the proliferating seedling itself, greatly reduce the economic burden for improving production efficiency, and adjust the harvest time and the accompanying advantageous production price. Thus, a method for growing and growing taro seedlings for planting can be realized, which is extremely advantageous in increasing farmers' willingness to produce taro. Hereinafter, representative examples will be described in detail so that the above-described method for growing and growing taro seedlings for planting according to the present invention can be more clearly understood.

[0025]

FIG. 1 is a schematic cross-sectional view for explaining the cultivation state in the process of promoting rooting and acclimatization, FIG. 2 is a process diagram for explaining a process of cutting and disinfecting a taro bud, and FIG. Is a schematic whole perspective view showing the cutting of the growing point and the primary aseptic culture, FIG. 4 is a process diagram showing a process of dividing from multiple shoots into tubers and placing them after the aseptic growth culture process, Temperature control,
As a facility capable of controlling the amount of light, the method of growing and growing a representative taro seedling for regular planting according to the present invention, which is implemented using a hotbed tunnel in a simple pipe house instead of a bio factory as in the past, will be specifically described. To do
This embodiment is as follows.

A potato with low consumption is selected, divided into about 20 g or a little larger, and immersed in a bleached powder solution diluted 300 times for about 1 minute, and then Benrate T wettable powder (trade name) 20 times Soak in the solution, after about 15 minutes, take out and shade, put the seed potatoes in a connection pot, fill with commercially available soil, and take 7-10 days at a temperature of about 25 ° C It is used as a taro for sprouting and cutting out the shoot apex.

As shown in the process diagram of FIG. 2, the germinated taro T is first thoroughly washed with a neutral detergent D by rubbing the entire surface thereof by hand as a preparatory operation before cutting off the shoot apex. After dropping, wash off the neutral detergent D with enough water and drain well in a Petri dish covered with two layers of filter paper. Then, the sprouted taro T is spread on aluminum foil and held firmly with tweezers on the filter paper. With a scalpel using a double-edged razor, the bud tip is about 2c below its base
The potato portion T1 is cut out so as to have a potato portion of about m, and the tip portion T1 is immersed in a 1% sodium hypochlorite solution N for about 15 minutes for sterilization and disinfection. ,
After the operation of washing with sterile water is repeated about three times, the operation of draining is completed, and preparation is made for the next cutting off of the shoot apex.

The sprout portion T1, which has been completely sterilized, is then dissected at the bud tip under a stereoscopic microscope on a sterile worktable.
A growth point (stem tip) 1 of about 3 mm is cut out, and as shown in a schematic whole perspective view showing the primary aseptic culture in FIG. 3, a primary culture medium K in a culture bottle 2, for example, an LS medium (3% sucrose,
pH 5.8) and the hormone condition was NAA 0.1 mg / L x BA 0.
Carefully transfer above 02mg / l at 25 ° C, 1
After aseptic cultivation for about 40 days with a photoperiod of 6 hours (6,000 Lux), the young leaves are grown to about 5 cm, and are grown into virus-free tubers.

The virus-free tubers grown by primary aseptic culture are further placed on a growth medium K1 in a conical beaker or culture bottle 2 for each tuber.
Aseptic cultivation is continued again for about 1 to 2 months so that the virus-free polyblasts 3 each having 5 to 7 tubers 31, 31,... The growth culture at this stage can be carried out by either shaking culture in a liquid medium or static culture in a solid medium.

In the shaking culture using the liquid medium, the basic medium is, for example, an LS medium (sucrose 3%, pH5.
8) A medium having a hormonal condition of 0.25 mg BA per liter was placed in a 100 ml conical beaker.
About 0 milliliter is injected, and about 1 month is applied for aseptic culturing while shaking at 100 rpm. In still culture using a solid medium, for example, LS is used as a basic medium.
Medium (3% sucrose, pH 5.8), support is agar 0.8
% Or gerritite 0.15% BA
In a culture bottle filled with 0.5 mg / liter medium, about 2 months, 25 ° C., 16 hours photoperiod (6,000 hours)
By sterile culturing under the condition of 0 Lux), virus-free polyblasts having 5 to 7 tubers necessary for the next step can be obtained. In addition, various instruments such as petri dishes, filter paper, aluminum foil, scalpels for cutting out shoot tips, culture bottles, conical beakers, etc., which are necessary for aseptic culture in the previous steps, must be used after being strictly sterilized and disinfected. The same applies to petri dishes and filter paper used in the sprouting operation.

Thus, the polyblasts 3, 3,..., Which are grown from 5 times to 14 to 16 times as large as the tuber through the primary culture, the primary culture, and the subsequent multiplication culture, and become virus-free, Removed from the culture bottle or conical beaker up to that time, each one tuber 3
1, 31, .... At that time, each tuber 31,3
1, remove roots and leaves that grow from “Potato + Petiole”
When handled in a state, the rooting and acclimatization steps of the virus-free tuber 31, which is one of the characteristic constitutions of the present invention, in the soil can be further assured, which is extremely convenient. It will be.

Each of the virus-free tubers 31, 31,..., Which has been divided into a “potato + stalk” shape, is filled with soil 5 for root acclimation in a cell tray 4 prepared in advance as a container for placing on a floor. The plant is planted for each of the cells 41. The planting is performed by making a hole in the root acclimating soil 5 in the cell 41, and shallowly plant the growing point so that the growing point is not hidden, that is, in the manner of “insertion”, and then lightly press the planting hole. Virus-free tuber 31, in all cells 41, 41, ...
The cell tray 4 in which each one of 31,... Has been planted, as shown in a schematic cross-sectional view for explaining the cultivation state in the rooting promotion and acclimatization process of FIG. The cell tray 4 is set in a water supply container 6 into which water has been injected 7 to a predetermined depth so that water can be absorbed from the bottom surface of the cell tray 4.
The root acclimatization soil 5 is Metromix 35.
0 (trade name) and vermiculite in a ratio of 1: 1 and mixed with Micro Long Total 10 as fertilizer.
A product obtained by adding a 0-day type (trade name) at a rate of 1.5 g / liter is used.

The cell tray 4 set in the water supply container 6
Preferably, for about one week in the initial stage, it is installed in a sealed hotbed tunnel 8 provided in a pipe house, for example, by covering it with wrap to surely prevent drying immediately after placing on the floor,
While keeping the temperature at 25 to 30 ° C., the light amount was adjusted by covering with non-woven fabric 9, sufficient moisture management was performed, and the wrap was gradually removed around one week after the lapse of about one week. Each virus-free tuber 31,3 in the period of about
Promotes rooting from 1, ....

After 2 weeks of the rooting promotion step,
While gradually removing the non-woven fabric 9, the sealed state of the hotbed tunnel 8 is gradually opened over a period of about two weeks, during which the temperature control at 20 to 25 ° C. and the case of the cell tray 4 are performed. In order to facilitate drying, sufficient water supply management is performed. After about one month, the hotbed tunnel 8 is completely removed and exposed to the outside air, and further about 15 months in the pipe house for about 1 month. By adjusting to the outside environment while maintaining the temperature range of ~ 20 ° C,
After the stems and leaves of the taro tuber 31 are gradually acclimated to the open air, irrigation management is also appropriately performed in the finishing stage to adjust to the planting conditions, and the virus-free tubers 31, 31,... In two months, the plant will eventually grow into a large number of excellent seedlings.

[0035]

[Effects] The method of growing and growing taro seedlings for planting according to the present invention, which is carried out by the above-described steps, is carried out from early to late September of the taro harvesting season. Examining the quality (tasting) and other factors, selecting seeds of superior strains, and extracting the shoot apex from the selected seeds at the optimal time calculated backward from the end of April to the beginning of May according to the production of fixed seedlings, Perform a primary aseptic culture by placing the bed,
Subsequently, after the growth of aseptic culture, 4
During about 2 months to the end of the month, the virus-free multi-bud was divided into tubers, and the tubers were directly shallowed in the manner of “cutting buds” on a medium placed in an environment free from aseptic culture. Planting to promote rooting, and then gradually acclimated to the external environment to grow into excellent fixed seedlings, planted in the field from late April to early May, planted in the field, prescribed cultivation management and pest control, In addition, since the dry season of the fattening season in July-August greatly affects the harvest, by performing appropriate furrow irrigation at this time, it is as competitive as the early digging taro harvesting season from early September to mid-season. During the season, taro can be reliably harvested.

The taro seedling for planting produced by the method of growing and growing according to the present invention is a compact seedling having no seed potato portion.
In particular, those produced by using a cell tray as a container for placing are difficult to remove soil and hair roots around the seedlings and are extremely excellent as fixed planting seedlings. Although the plant height is rather small in comparison, the potatoes grow faster and have a strong tendency to enlarge,
It is very suitable for early digging and high harvesting. Yamagata shows that the test cultivation results show that the yield is 1.8 times larger than that of conventional seed potato seedlings, and the ratio of large balls is considerably higher. It has been proved by the test results of the prefectural horticultural testing station.

The results of this test show that, in the case of a conventional seed potato seedling, the growth of the foliage takes precedence as the vegetative growth.
In the case of the virus-free planting method of the present invention, a large number of Coulomb seedlings are produced from the selected excellent seeds, while the number of the seedlings is delayed and the number does not increase so much. Coulomb seedlings are all of excellent quality,
Because there is no potato (nutrition) part, plant instinct to leave offspring, prioritize the way to make potatoes before nutrients from the roots are passed on to the growth of foliage, as a result, The yield increases,
Conversely, it is expected that the plant height will be inferior to that of conventional seed potato seedlings.

The method of growing and growing taro seedlings for planting according to the present invention differs from the conventional root acclimation process in that many tubers, which have been virus-free by shoot apex culture, are released from the aseptic culture environment. It is a plant that is directly shallowly planted in the rooting acclimation soil below, and can adopt an innovative root acclimation process that controls water supply, prescribed temperature control, and light intensity. It is not necessarily limited to the limited facility environment inside, so it is also possible to produce planted seedlings in a simple facility such as a pipe house. It is not necessary to prepare suitable facilities, and the great advantage that the economic burden to increase production efficiency can be greatly reduced is obtained, and it is extremely easy to provide planted seedlings at the harvest time. Thereby achieving the Xiu features.

As a result, for example, as a new taro digging in early September, which coincides with the beginning of the “Imoni-kai” event in Yamagata Prefecture, which is a large consumption area of taro, it is possible to make adjustment shipments from local farmers. It is no longer necessary to rely on the early pick-up of new taro from the region, and the meaning of the hometown event is strengthened as a `` potato stewed by local taro '', which contributes to the promotion of tourism and contributes to the promotion of the region, and above all, As a result, it is possible to secure a favorable production price as a fast-digging new taro, stimulate the willingness of local farmers to produce, stabilize farm management as a new production item, and greatly contribute to regional revitalization. You can expect it.

In particular, according to the method of growing and growing taro seedlings for planting, which is representative of the present invention described in the embodiments, high-quality planting can be performed by a simple facility such as a pipe house without preparing special facilities. It is possible to ensure the large-scale cultivation and propagation of seedlings, not only for traditional taro producers, but also for producers who are going to newly enter, without excessive burden on initial investment. It will be possible to work on attractive taro production that can be dug and harvested and can expect a sufficient increase in yield, thereby increasing the number of taro producers and exerting its power in stabilizing the supply system of high quality taro. .

As described above, the method for growing and growing taro seedlings for planting according to the present invention is based on an extremely novel structure that focuses on the unique plant characteristics of taro, and enables the intended purpose to be achieved universally. Yes, liberalization of agricultural products, change of agricultural administration, etc.
This technology is extremely effective in stabilizing the management base of farmers facing a severe environment due to various factors, and has been highly evaluated by many farmers under the guidance of the responsible administrative organization and related research and research institutes, and taro production It is expected to greatly contribute to the spread and expansion of.

BRIEF DESCRIPTION OF THE DRAWINGS The drawings are for illustrating a typical embodiment of the method for growing and growing taro seedlings for planting of the present invention.

FIG. 1 is a schematic cross-sectional view for explaining a cultivation state in a rooting promotion and acclimatization process.

FIG. 2 is a process diagram for explaining a process of cutting and disinfecting a taro bud tip.

FIG. 3 is a schematic whole perspective view showing cutting of a growing point and primary aseptic culture.

FIG. 4 is a process chart showing a process of dividing from multiple buds into tubers and placing them on a bed after a sterile growth culture process.

[Description of Signs] 1 Growth Point (Stalk Apex) 2 Culture Bottle 3 Virus-Free Multi Buds 31 Same Tuber 4 Container for Placement (Cell Tray) 41 Same Cell 5 Soil for Root Conditioning 6 Water Supply Container 7 Water Injection 8 Hotbed Tunnel 9 Non-woven fabric D Neutral detergent N 1% sodium hypochlorite solution T Taro T1 Same bud tip

 ──────────────────────────────────────────────────続 き Continuing from the front page (72) Inventor Hiroshi Isuzugawa 434, Yamagata Prefectural Horticultural Testing Center, Shimae-shi, Yamagata Prefecture (72) Inventor Narumi Matsuda 434 Yamagata Prefectural Horticultural Testing Center, Sagae-shi, Yamagata Prefecture (72) Inventor Yamakawa Ryuhei 2-8-1, Matsunami, Yamagata City Agriculture, Forestry and Fisheries Department, Agriculture and Livestock Production Promotion Division, Yamagata Prefecture (72) Inventor Mitsuharu 1366, Kadozawa, Shinjo City, Yamagata Prefecture Yamagata Agricultural Research and Training Center (72) Inventor Nari Sato Yamagata 2-8-1, Ichimatsunami, Agriculture, Forestry and Fisheries Division, Agriculture, Forestry and Fisheries Department, Yamagata Prefecture (72) Atsushi Oki, 2034 Kanazawa, Shinjo City, Yamagata Prefecture F-term (reference) in Shinjo Agricultural Improvement Center, Yamagata Prefecture 2B022 AA01 AB13 BA11 BA21 BB10 DA19 EA10 2B030 AA02 AB03 AD05 CB02 CD03 CD06 CD10 CD13 CD15 CD21

Claims (6)

[Claims] Claims: 1. A taro growing point is aseptically removed and cultivated in a shoot apex to grow and proliferate a large number of virus-free polyblasts from one growing point, and then to divide each individual bud into tubers. In a facility where each tuber is individually planted in a shallow plant form in a soil in a container that can absorb water on the bottom surface in an environment free from aseptic culture, For each planted container, perform water management, temperature management, and light intensity management by bottom water supply for a predetermined period to promote rooting from taro tubers, and then gradually grow into a constant seedling while gradually acclimating to outside air. A method for growing and growing taro seedlings for planting, characterized by having done this. Bed 2. A method of cultivating and growing a large number of polyblasts virus-free from one growth point by removing the taro growth points under aseptic conditions and cultivating the shoot apex, and separating the individual buds from each tuber. And each tuber is directly planted in the soil as a shallow planting for each cell of the cell tray filled with the soil under the environment released from the aseptic culture, temperature,
In a facility where light quantity can be controlled, for each planted cell tray, moisture control and temperature control by bottom water supply, light quantity control are performed for a predetermined period to promote rooting from taro tubers, while gradually acclimating to outside air A method for growing and growing taro seedlings for constant planting, wherein the method is adapted to grow to constant planting seedlings. 3. The taro growing point is aseptically removed and cultivated in the shoot apex to grow and proliferate a large number of virus-free polysprouts from one growing point, and to separate the individual buds from each tuber. And each of the tubers is directly planted in the soil as a shallow plant for each cell of the cell tray filled with the soil under an environment freed from aseptic culture, and the cell tray planted in the hotbed tunnel Every time, after performing water management, temperature management, and light quantity management by bottom water supply for a predetermined period to promote rooting from taro tubers, after finishing the management to gradually acclimate to the outside air, completely remove the hotbed tunnel, A method for growing and growing taro seedlings for fixed planting, wherein the plant is grown to a fixed planting seedling with proper irrigation management. 4. The taro growing point is aseptically removed and cultivated in the shoot apex to obtain a virus-free multibud, and the tuber of the multibud is divided into individual tubers and then sterilized again. By further multiplying the split shoots, the
A process in which many buds are grown and proliferated from the growth point. The buds obtained from the above process are divided into tubers, and each of them is released from an aseptic culture. In each cell of the cell tray filled with the soil, the process of planting the growing point directly in the soil as a shallow planting shape that does not bury the growing point, for each planted cell tray, set in a water supply container that can supply water to the bottom , A process of promoting rooting from taro tubers by installing it in a sealed hotbed tunnel for a predetermined period under the control of temperature, light quantity, moisture, etc., and then gradually releasing the sealed state of the hotbed tunnel ,
The process of gradually acclimating the foliage to the outside air while maintaining the temperature management, light amount management and water supply management over a predetermined period, the process of completely removing the hotbed tunnel, and growing the plant to a fixed seedling while appropriately controlling irrigation. A method for growing and growing taro seedlings for planting, comprising the steps of: 5. A taro growing point is aseptically removed and cultivated in a shoot apex to obtain a virus-free multibud, and the tubers of the multibud are divided into individual tubers and then sterilized again. By further multiplying the split shoots, the
A process in which many buds are grown and proliferated from the growth point. The buds obtained from the above process are divided into tubers, and each of them is released from an aseptic culture. In each cell of the cell tray filled with the soil, the process of planting the growing point directly in the soil as a shallow planting shape that does not bury the growing point, for each planted cell tray, set in a water supply container that can supply water to the bottom 25, installed in a closed hotbed tunnel prepared in a pipe house.
Approximately 2 under temperature control of about 30 ° C, light quantity control, and water supply control
Growing for about a week to promote rooting from taro tubers,
After that, the sealed state of the hotbed tunnel is gradually released, and the process of gradually acclimating the foliage to the outside air while maintaining the temperature control of about 20 to 25 ° C., the light quantity control, and the water supply control over a predetermined period, completely. A method for growing and growing taro seedlings for fixed planting, comprising the steps of: removing the hotbed tunnel and growing the planted seedlings while maintaining proper irrigation; 6. A shoot-free cultivation for virus-free, multiple buds from one growing point, take out from the culture room, divide it again into individual pieces, and add In the process of planting the tubers directly on the soil in a shallow planting where the growth points are not buried, the tubers are divided and planted one by one so that rooting from the tubers is further promoted in the next process A method for growing and growing taro seedlings for planting according to any one of claims 1 to 5, wherein the leaves and roots are removed from the seedlings, the tuber and the petiole are removed, and the seeds are inserted into the soil and shallowly planted in the manner of buds. .