KR20220121530A - Hydroponics method of tissue cultured taro - Google Patents

Abstract

The present invention relates to a hydroponic cultivation method of tissue cultured Colocasia esculenta, which plants mother bulb in bed soil to induce bud production, after removing the outer skin of the bud produced, forms shoots in a medium to which BAP, kinetin, IAA and the like are added, grows seedlings in a medium to which BAP, kinetin and the like are added, uproots the grown seedlings, and after transplantation into a culture bed, supplies a nutrient solution with pH 5.7 to the seedlings six times a day every day for 120 to 150 days at 80 to 120 mL per week at 80 to 100 minute intervals. The hydroponic cultivation method of the tissue cultured Colocasia esculenta shows excellent productivity of an underground part of the Colocasia esculenta.

Description

Hydroponics method of tissue cultured taro {HYDROPONICS METHOD OF TISSUE CULTURED TARO}

The present invention relates to a method for hydroponic cultivation of tissue cultured taro, and more particularly, to a method for hydroponically cultured tissue cultured taro formed using taro seedlings.

Taro (土卵, Colocasia esculenta ) is a perennial plant of the family Cheonnamaceae, and its origin is tropical Asia such as India and Indonesia, and it is widely cultivated in tropical regions such as India, Sri Lanka, Sumatra, and the Malay Peninsula. It is treated as a staple food in many islands in the Pacific region. It is mainly vegetatively propagated. It is an annual in Korea. Even if it blooms, it does not bear fruit due to the low temperature and dry climate, but it is a perennial in the tropics. The scientific names are generally variants of Colocasia , C. var esculenta and C. var. classified as antiquorum .

Looking at the botanical characteristics of taro, the stem hardly grows in the ground and becomes enlarged to become a kohlrabi or tuber. The leaves are 1-1.5m long, and the leaves are lip-shaped, egg-shaped, or heart-shaped, 30-50cm long and 25-30cm wide. The surface is slippery. The flowering habit is disappearing during cultivation after a long period of time, but sometimes flowers bloom in the fall of the hottest year. Flowers hang at the end of stalks that emerge from between petioles in a stalk inflorescence, and are wrapped in yellow flames of 25-30cm in length and 6cm in width. A female flower hangs at the bottom of the inflorescence, a male flower above it, and a silent flower at the end.

Native species of Korea are usually early-growing, green stems, several young taro and small eggs. Tubers are divided into baby taro and mother taro, and the mother taro has large eggs and a strong astringent taste, so it is almost impossible to eat. The cultivation characteristic of taro is a thermophilic plant and grows well in high temperature and high humidity. There are very few pests and diseases, and although it blooms occasionally, it does not produce seeds, so the kohlrabi is planted around April~May. In addition, the use of chemical fertilizers or pesticides is low, moisture resistance is strong, it is relatively well tolerated in shade, weak to drying, and can withstand low temperatures of about 5 ℃ but weak to frost. It exhibits its original characteristics in high temperature and humidity conditions, and the minimum temperature for germination is 15℃ and the optimum temperature for growth is 25~30℃. Mogu has little edible value and is mainly cultivated as jagu. In Korea, research on cultivation methods such as mulching is in progress, but classification and breeding projects of varieties are scarce. .

The above-ground growth characteristics of taro can be investigated through leaf length, leaf width, leaf length and number of leaf blades. Referring to FIG. 1, the petiole is the part that attaches the leaf body to the stem or branch, and the petiole represents the length from the ground surface to the petiole, and the leaf width means the width of the widest part perpendicular to the growth direction of the leaf length. is the length of the leaf, excluding the petiole, from the top of the leaf to the tip of the bottom.

The nutritional component of taro is rich in various trace elements and minerals such as calcium and potassium, so consumption is increasing as it is known as a functional food such as antipyretic, inflammation treatment, neuralgia relief, eohyeol, high blood pressure, constipation, and anti-aging.

Table 1 below shows the nutrient content of taro (boiled standard, tuber 100g).

carbohydrate protein lipid calcium sign vitamin calorie 12.5g 2.6g 0.2g 17mg 32mg 3mg 60 kcal

And, Table 2 below shows the nutrient content of taro root (100 g).

carbohydrate protein lipid calcium sign steel potassium calorie 3.5g 0.5g 0.1g 270mg 19mg 1.3mg 240mg 15 kcal

As can be seen in Tables 1 and 2 above, despite the nutritional and various functional characteristics of taro, there are not many domestic cultivars and continuous cultivation is performed through vegetative propagation using locust. This has an impact on quality and productivity.

An object of the present invention is to provide a tissue culture method and a hydroponic culture method of taro having excellent underground growth of taro and excellent productivity of taro seed bulb.

In the hydroponic cultivation method of tissue cultured taro according to an embodiment of the present invention, (S1) taro seedlings are planted in sterilized medium at 25±2° C. for 5 to 7 days, for 15 to 17 hours a day, 2,000 to 3,000 lux Inducing bud formation by irradiating with light, collecting the eye, removing the integument of the collected eye, and disinfecting the eye from which the integument has been removed, BAP 0.5-10 mg, kinetin 0.5-10 The sterilized eyes are cultured in MS (Murashige & Skoog) basal medium or LS (Linsmaier & Skoog) medium to which one or more selected from the group consisting of mg and IAA 0.05 to 1 mg is added to form shoots, and BAP and kinetin After the seedlings are grown by propagating the shoots in LS medium to which one or more selected from the group consisting of preparing tissue cultured taro seedlings; (S2) planting the tissue cultured taro seedlings in a culture bed; (S3) supplying a nutrient solution having a pH of 5.7 6 times/day at intervals of 80 to 100 minutes at 80 to 120 mL per week of the taro seedlings every day for 120 to 150 days after planting; and (S4) harvesting after 120 to 150 days have elapsed after the planting.

According to the hydroponic cultivation method of tissue-cultured taro according to the present invention, it is different from conventional taro in the above-ground growth characteristics, and in particular, taro having excellent productivity in terms of the number of seeds and total weight in the underground part of taro can be cultivated.

1 shows a method for measuring the growth of taro above ground.
2 is a photograph showing taro seedlings rooted in a medium according to an embodiment of the present invention.
Figure 3 is a photograph showing the state of the tissue cultured taro that is grown hydroponically according to an embodiment of the present invention.
4 is a photograph taken at the same height of taro of Comparative Example (a) and Example (b), respectively.
5 is a graph showing the results of examining the leaf length, leaf length, and side width of taro grown according to Comparative Examples 1 and 2 and Examples 2 months after planting.
6 is a graph showing the results of examining the leaf length, leaf length, and side width of taro grown according to Comparative Examples 1 and 2 and Examples 5 months after planting.
7 is a graph showing the results of investigating the number of leaf blades 2 months after planting (1st) and 5 months (2nd) after planting of taro grown according to Comparative Examples 1 and 2 and Examples.
8 is a graph showing the yield per week 5 months after planting of taro grown according to Comparative Examples 1 and 2 and Examples.

Hereinafter, each configuration of the present invention will be described in more detail so that those of ordinary skill in the art can easily implement it with reference to the drawings, but this is only an example, and the scope of the present invention is not Not limited by the following:

In the hydroponic cultivation method of tissue cultured taro according to the present invention, (S1) taro bulbs are planted in sterilized medium at 25±2° C. Irradiation induces bud formation, collects the eye, removes the integument of the collected eye, and disinfects the eye from which the integument has been removed, BAP 0.5-10 mg, kinetin 0.5-10 mg, and IAA A shoot is formed by culturing the sterilized eyes in MS (Murashige & Skoog) basal medium or LS (Linsmaier & Skoog) medium to which one or more selected from the group consisting of 0.05 to 1 mg is added, and a group consisting of BAP and kinetin After the seedlings are grown by propagating the shoots in LS medium to which one or more selected from preparing egg seedlings; (S2) planting the tissue cultured taro seedlings in a culture bed; (S3) Every day for 120 to 150 days after planting, at 80 to 120 mL per week of the taro seedlings, at 80 to 90 minute intervals, a nutrient solution having an EC (Electrical Conductance) of 1.5 ds/m and a pH of 5.7 is 6 supplying times/day; and (S4) harvesting after 120 to 150 days have elapsed after the planting.

Hereinafter, the hydroponic cultivation method of tissue cultured taro according to the present invention will be described by dividing each step.

Prior to this, a method for preparing tissue cultured soil seedlings will be described in detail.

(S1) Steps to prepare tissue cultured soil seedlings

1) Inducing bud formation

This step is a step to induce eye production by planting taro bulbs in sterile soil. After planting the taro bulbs harvested in the same year in sterilized soil, it induces the formation of snow around the cotyledons grown in the center of the taro bulbs. At a temperature of 25±2℃, for 5-7 days. , for 15 to 17 hours a day, irradiating light at 2,000 to 3,000 lux to promote eye development. Centering on the cotyledon, when the annular eye no longer develops or when the cotyledon grows more than 3 cm, the cotyledon is removed to induce the development of additional eyes. Eyes that have grown more than 5 mm are collected using a sterilized scalpel and used as a culture material.

The reason for inducing eye development in the above way is the part that contains meristem that can be a material for in-flight culture of the eye. Therefore, the generation of eyes around the hair bulb is suppressed. In addition, this is to induce the generation of as many eyes as possible while minimizing the contaminants transmitted from the outside.

2) Disinfecting the eyes from which the integument has been removed

In this step, the eye generated from the taro bulb is collected, the skin of the collected eye is peeled and removed, and then the eye from which the skin has been removed is disinfected. Remove the outer skin by peeling 2-3 skins close to the dark green of Secondary disinfection, followed by washing with distilled water, secondary disinfection with alcohol, and final sterilization using sodium hypochlorite again.

The reason for sterilizing the eyes by the above method is to increase the production rate of shoots generated from the eyes and to ensure healthy culture by disinfecting and sterilizing external contaminants buried in the eyes collected from taro hairballs to remove contaminants. .

The specific method of disinfecting and sterilizing the eyes is disinfected by immersing the collected eyes in a commercially available general commercial seed disinfectant solution (hypochlorite, hypochlorite) for 5 to 30 minutes, and then washing with distilled water 3 to 5 times. , again immersed in 70±5% alcohol for 1 to 3 minutes, and then final sterilized in 0.1 to 5% sodium hypochlorite.

3) Forming shoots by culturing sterilized eyes

As described above, the sterilized eyes should be cultured in shoot formation medium, in which case the shoot formation medium for forming shoots in the eyes is MS (Murashige & Skoog) basic medium or LS (Linsmaier & Skoog) medium, BAP (benzylaminopurine) , kinetin, and any one or more selected from the group consisting of IAA may be added. Specifically, any one of BAP and kinetin is added to any one of MS basal medium or LS medium, or BAP or kinetin is added to any one of MS basal medium or LS medium, and IAA is added After preparing a shoot formation medium by adding to it, it can be prepared by adding sucrose and phyta gel.

Tables 3 and 4 below show the number of shoots formed according to the shoot formation medium.

division MS Basic Medium 1L + BAP 0.5~10mg MS basic medium 1L + kinetin 0.5~10mg MS Basic Medium 1L
+BAP 0.5~10mg
+IAA 0.05~1mg MS Basic Medium 1L
+ kinetin 0.5~10mg
+IAA 0.05~1mg shoot
formation
Count increase
ceremony
Work 3 weeks 0.9 ±0.3 0.8 ± 0.3 1.1 ± 0.6 0.9 ± 0.6 4 weeks 1.4 ±0.5 1.2 ± 1.4 1.8 ± 0.6 1.6 ± 1.2 5 weeks 2.2 ±1.2 1.8 ± 0.6 2.8 ± 0.5 2.1 ± 0.5

division LS medium 1L +BAP 0.5~10mg LS medium 1L +kinetin 0.5~10mg LS Medium 1L
+BAP 0.5~10mg
+IAA 0.05~1mg LS Medium 1L
+ kinetin 0.5~10mg
+IAA 0.05~1mg shoot
formation
Count increase
ceremony
Work 3 weeks 1.5 ± 0.1 1.2 ± 0.5 1.6 ± 0.2 1.0 ± 0.4 4 weeks 2.3 ± 1.2 2.0 ± 0.8 2.8 ± 1.3 2.6 ± 1.0 5 weeks 3.4 ± 1.5 3.2 ± 0.3 3.9 ± 0.4 3.6 ± 1.5

The BAP and kinetin mainly play the role of growth regulators necessary for the growth of shoots of seedlings. In 1L of either MS basal medium or LS medium, each of BAP and kinetin is added in an amount of 0.5 to 10 mg, and the amount of IAA added is 0.05 to 1 mg. The sucrose serves as an energy source (growth hormone) necessary for the formation of new shoots in the eyes, and Phyta gel serves to gel (solidify) the shoot formation medium.

In 1L of either MS basal medium or LS medium, the amount of sucrose added is 20-50 g, and the amount of phyta gel added may be 2-5 g. If the amount of sucrose added is less than 20 g, the eyes or shoots are normally If the energy source (carbon source) required for growth is insufficient, so that it cannot grow normally, and when it exceeds 50 g, the energy source required for the normal growth of the germinated eye is excessive and the problem of overgrowth may occur.

In addition, phyta gel is a liquid basic medium, and the liquid is gelled (solidified) so that the eyes or shoots do not flow and grow stably fixed in place. When less than 2 g is used, the liquid MS basic medium Alternatively, the LS medium does not gel, and if more than 5 g is used, the liquid MS basic medium or LS medium becomes too hard, which may cause problems that interfere with the growth of eyes or shoots.

4) Step to grow seedlings by proliferating shoots

The shoots formed by the above method are divided one by one and transferred to the shoot growth medium and propagated. At this time, the shoot growth medium for propagating the shoots is as shown in Table 5 below, in the LS medium, BAP (benzylaminopurine) or It can be prepared by adding any one of kinetin.

division LS Medium 1L LS Medium 1L BAP 1mg BAP 5mg BAP 10mg kinetin 1mg kinetin 5mg kinetin 10mg 3 weeks 1.51±0.2cm 1.53±0.28cm 1.42±0.3cm 1.10±0.5cm 0.51±0.4cm 0.44±0.3cm 4 weeks 2.58±0.1cm 2.77±0.5cm 2.32±0.2cm 2.05±0.4cm 1.68±0.5cm 0.70±0.2cm 5 weeks 3.72±0.3cm 3.52±0.2cm 2.92±0.1cm 3.12±0.5cm 2.05±0.8cm 1.15±0.3cm

The BAP and kinetin mainly serve as growth regulators necessary for shoot growth of seedlings, and the amounts of each of BAP and kinetin added to 1 L of LS medium are 1 to 10 mg.

If less than the above amount is used or used in excess, shoot formation and proliferation of seedlings do not occur normally, resulting in slow formation and proliferation of shoots or abnormal growth, and then leaves and roots of seedlings There may be a problem in that growth cannot be maintained in a balanced state.

The shoot growth medium may be prepared by adding only BAP or kinetin to the LS medium.

When the shoot growth medium is prepared in the same way as above, each shoot node divided into units of each node is transferred to the prepared shoot growth medium and at a temperature of 22 to 27 ° C, for 3 to 5 weeks, 15 to 17 hours a day By irradiating light at 2,000-3,000 lux during the period, the shoots are multiplied and the seedlings are grown.

As shown in Table 5 above, the best results for shoots of seedlings were that BAP was added to the LS medium, and the addition of kinetin to the LS medium showed that shoot growth was inhibited as the concentration increased too much. When BAP was added to the LS medium, it was most preferable to grow for 4 to 5 weeks.

5) Rooting the grown seedlings

After the node seedlings are grown by propagating the shoots by the above-described method, the grown seedlings are transferred to the seedling rooting medium to induce rooting. In this case, the seedling rooting medium may be prepared by adding any one of indole butyric acid (IBA) and naphthalene acetic acid (NAA) to the LS medium.

Both of the IBA and NAA serve to promote rooting and growth of node seedlings, and it is preferable to add 1 to 10 mg of any one of IBA and NAA to 1 L of LS medium.

When the seedling rooting medium is prepared, if the amount of the additive is used less than or exceeding the numerical range, the rooting of the seedlings grows too slowly or overgrowth, so that healthy rooting is not possible. It is preferable to satisfy the numerical range.

Through the above process, tissue cultured taro seedlings are prepared. 2 shows a state in which taro according to an embodiment of the present invention is rooted in the seedling rooting medium. And Table 6 below shows the length of the seedlings grown according to the type of seedling rooting medium.

division LS medium + IBA (mg/L) LS medium + NAA (mg/L) One 2 5 10 One 2 5 10 6 weeks 3.5±
0.4cm 2.83±
0.6cm 2.32±
0.3cm 1.50±
0.5cm 2.6±
0.3cm 2.15±
0.2cm 1.92±
1.2cm 1.40±
0.6cm 8 weeks 7.2±
1.2cm 4.92±
0.3cm 3.50±
0.3cm 1.85±
0.4cm 5.3±
0.8cm 3.52±
0.2cm 2.50±
0.1cm 1.65±
0.2cm

(S2) planting tissue cultured taro seedlings in the culture bed

The rooted taro seedlings are planted in a culture bed, and the culture bed may utilize, for example, a strawberry cultivation bed.

It is preferable to plant the taro at a wide interval of 30 cm or more in the process of planting. For example, it can be planted at intervals of 30 cm. In the case of planting at an interval of less than 30 nm, the leaves overlap between neighboring taro, which may cause a problem that the taro does not receive sufficient sunlight.

The planting can be done, for example, around the beginning of June.

(S3) Cultivation stage after planting

For 120 to 150 days after planting, 80 to 120 mL, preferably 100 mL, of the taro seedling per week every day for 120 to 150 days after planting, a nutrient solution having a pH of 5.7 is supplied 6 times/day at intervals of 80 to 100 minutes, preferably 90 minutes.

It is desirable to apply different environments by dividing the period from planting to harvest into four quarters from the cultivation stage.

As for the temperature, in one embodiment, the average daily minimum temperature for the first quarter is 14.8°C, the average daily maximum temperature is 40.1°C, and the average daily temperature for the first quarter is 26.99°C; The average daily minimum temperature for the second quarter was 19.6℃, the average daily maximum temperature was 40.8℃, and the average daily temperature for the second quarter was 26.58℃; The average daily minimum temperature for the third quarter was 23.7℃, the average daily maximum temperature was 47.8℃, and the average daily temperature for the third quarter was 31.48℃; The average daily minimum temperature for four quarters may be 13.5°C, the average daily maximum temperature may be 45.9°C, and the average daily average temperature for four quarters may be set to 25.25°C, but the present invention is not limited to the above embodiment. From planting to harvest before harvest, the appropriate temperatures required depend on the degree of growth of taro, and when the temperature is controlled as described above, the underground growth of taro may be the best.

In one embodiment, the average daily minimum humidity of the first quarter is 30.2%, the average daily maximum humidity is 92.8%, the average daily average humidity is 73.31%; The average daily minimum humidity for the second quarter was 35.2%, the average daily maximum humidity was 91.8%, and the average daily average humidity for the second quarter was 78.72%; The average daily minimum humidity in the third quarter was 27.3%, the average daily maximum humidity was 92.0%, and the average daily average humidity was 70.53% in the third quarter; The average daily minimum humidity of 4 quarters may be 27.4%, the average daily maximum humidity may be 90.1%, and the 4 quarter average of the daily average humidity may be 74.97%, but the present invention is not limited to the above embodiment. Appropriate humidity is different depending on the growth degree of taro after planting until harvest, and when the humidity is controlled as described above, the growth of the underground part of taro may be the best.

The EC (Electrical Conductance, electrical conductivity) of the nutrient solution supplied to the taro may be varied and supplied for each quarter. The EC is the reciprocal of the electrical resistance of the nutrient solution and represents the total concentration of solute dissolved in the nutrient solution. In one embodiment, the EC of the nutrient solution supplied to taro is an average EC of 1.91 ds/m in the first quarter, an average EC of 1.57 ds/m in the second quarter, an average EC of 1.63 ds/m in the third quarter, and an average EC of the fourth quarter. is 0.96 ds/m, but the present invention is not limited to the above embodiment, and all of the taro growth effects are excellent in the range of ±0.20 ds/m for each average EC. The appropriate EC of the nutrient solution required varies depending on the growth degree of taro after planting until harvest, and if the EC of the nutrient solution supplied to the taro is adjusted every quarter as in the above embodiment, the growth of the underground part of the taro may be excellent.

In one embodiment, the composition of the nutrient solution is nitrogen (N) 5.8 me/L, phosphorus (P) 1.7 me/L, potassium (K) 3.4 me/L, calcium (Ca) 2.2 me/L, regardless of the branching, It may include magnesium (Mg) 1.1 me/L and sulfur (S) 1.2 me/L, but is not limited to the above example, and the concentration can be adjusted within ±0.1 me/L. When the nutrient solution of the same composition as in the above embodiment is supplied to taro, the growth of the underground part of taro may be particularly excellent.

It is helpful to promote taro growth by arbitrarily controlling the daily average cumulative solar radiation that is irradiated to the taro during the cultivation process. As an example, the daily average cumulative insolation can be divided into ⁴ quarters from planting to harvest and applied differently for each quarter. ² and 3 quarters of 662.19 J/cm ² and the daily average cumulative insolation of the 4th quarter may be made to be 641.15 J/cm ² , but it is not limited to the above example. In contrast, within the range of ±50 J/cm ² , the growth of the underground part of taro is excellent.

(S4) Harvesting after planting

The hydroponically cultivated taro can be harvested after 120 to 150 days from the date of planting, preferably after 120 to 150 days. The taro planted in the open field is the most nutrient-rich, and a large yield can be obtained by harvesting when the length of the petiole is preferably 55-85 cm among 120 to 150 days from the planting date.

Figure 3 is a photograph showing the appearance of the tissue cultured taro cultured hydroponically according to an embodiment of the present invention.

[Experimental Example 1: Comparison of growth characteristics of taro above ground]

According to the present invention, 120 strains of tissue cultured taro (Example) cultivated in the open field and 120 strains of Gokseong Jonggu (Comparative Example 1) and Goesan Jonggu (Comparative Example 2), each of which are one of the conventional varieties, were grown in a generally cultivated method. Thus, the leaf length, leaf length, leaf width, and number of leaf blades were measured twice for growth. The first growth survey was carried out 2 months after planting, and the second growth survey was carried out 5 months after planting. Tables 7 and 5 below show the average values of the primary growth investigation of the above-ground parts of taro of the Examples and Comparative Examples that were hydroponically grown, and Tables 8 and 6 below show the secondary above-ground parts of the hydroponically grown taro of the Examples and Comparative Examples. The average value of the growth survey is shown. 7 is a graph showing the average value of the number of leaf blades irradiated in the first and second growth surveys at once.

Jasper length (cm) Leaf length (cm) Leaf width (cm) Jasper (dog) Comparative Example 1 78.6±4.7 32.7±3.1 26.2±2.8 8.8±2.4 Comparative Example 2 81.8±5.1 33.6±1.9 27.4±1.5 7.8±2.3 Example 59.1±6.1 29.1±3.3 23.6±2.9 10.0±1.9

Jasper length (cm) Leaf length (cm) Leaf width (cm) Jasper (dog) Comparative Example 1 98.9±12.0 44.1±4.6 37.4±4.6 7.6±1.1 Comparative Example 2 110.6±2.2 41.3±4.3 35.8±1.7 6.8±2.6 Example 69.0±11.9 36.8±7.0 27.0±5.4 10.2±1.5

Through the primary growth survey, the Example showed that the leaf blade length was significantly shorter than that of Comparative Examples 1 and 2, and specifically, the Example was found to be 24.8% shorter than that of Comparative Example 1.

However, there was no significant difference in leaf length and leaf width.

7 is a graph showing the results of examining the number of leaf blades of taro grown according to Comparative Examples 1 and 2 and Examples.

The average number of leaf blades was 8.8 pieces/week in Comparative Example 1, 7.8 pieces/week in Comparative Example 2, and 10.0 pieces/week in Example 1 during the first irradiation. Example 2 was investigated at 6.8 pieces/week and Example 10.2 pieces/week. Compared with Comparative Example 1, the number of leaf blades of Example was 22% and 33.3% for the 1st and 2nd rounds, respectively, indicating a significant difference with about 3 leaf blades per week.

The number of jagers is related to the number of altorans, and as the number of jagers increases, the number of altorans tends to increase. Therefore, through the above results, it means that the number of altorans of the example is larger than the number of altorans of the comparative example on average.

[Experimental Example 2: Comparison of taro underground growth characteristics]

8 is a graph comparing the underground growth characteristics of Comparative Example 1 (Gokseong Jongsphere), Comparative Example 2 (Goesan Jongsphere) and Examples grown in Experimental Example 1, and is shown in Table 9 below.

Number of species (pcs) Gross weight (g) Weight per piece (g) Comparative Example 1 16.8 277 17 Example 45.6 649 14

Referring to Table 9 and FIG. 8, compared to the comparative example, the number of seed bulbs, the total weight, and the weight per piece of the Example all show higher values. The yield is significantly higher.

Although preferred embodiments of the present invention have been described in detail above, the scope of the present invention is not limited thereto, and various modifications and improvements by those skilled in the art using the basic concept of the present invention defined in the following claims are also present. It belongs to the scope of the right of the invention.

Claims (7)

(S1) Planting taro bulbs in sterilized soil at 25±2° C. and irradiating light at 2,000 to 3,000 lux for 15 to 17 hours a day for 5 to 7 days to induce bud formation, After removing the integument of the collected eye, disinfecting the eye from which the integument has been removed, any one or more selected from the group consisting of BAP 0.5-10 mg, kinetin 0.5-10 mg, and IAA 0.05-1mg The sterilized eyes are cultured in MS (Murashige & Skoog) basal medium or LS (Linsmaier & Skoog) medium to form shoots, and in the group consisting of BAP and kinetin After the seedlings are grown by propagating the shoots in LS medium to which one or more selected at least one selected, the grown seedlings are rooted in LS medium to which at least one selected from the group consisting of IBA and NAA is added to tissue culture taro. preparing the seedlings;
(S2) planting the tissue cultured taro seedlings in a culture bed;
(S3) supplying a nutrient solution having a pH of 5.7 6 times/day at intervals of 80 to 100 minutes at 80 to 120 mL per week of the taro seedlings every day for 120 to 150 days after planting; and
(S4) the step of harvesting after 120 to 150 days have elapsed after the planting; According to claim 1,
In the step (S2), the tissue cultured taro seedlings are planted at 30cm intervals. The method of claim 1,
The step (S3) is divided into four quarters and the temperature is different,
The average daily minimum temperature for the first quarter was 14.8℃, the average daily maximum temperature was 40.1℃, and the average daily average temperature for the first quarter was 26.99℃;
The average daily minimum temperature for the second quarter was 19.6℃, the average daily maximum temperature was 40.8℃, and the average daily temperature for the second quarter was 26.58℃;
The average daily minimum temperature for the third quarter was 23.7℃, the average daily maximum temperature was 47.8℃, and the average daily temperature for the third quarter was 31.48℃; and
The hydroponic cultivation method of tissue cultured taro, characterized in that the average daily minimum temperature of 4 quarters is 13.5 ° C, the average daily maximum temperature is 45.9 ° C, and the average daily temperature is 25.25 ° C.; According to claim 1,
The step (S3) is divided into 4 quarters to vary the humidity,
The average daily minimum humidity in the first quarter was 30.2%, the average daily maximum humidity was 92.8%, and the average daily average humidity was 73.31% in the first quarter;
The average daily minimum humidity for the second quarter was 35.2%, the average daily maximum humidity was 91.8%, and the average daily average humidity for the second quarter was 78.72%;
The average daily minimum humidity in the third quarter was 27.3%, the average daily maximum humidity was 92.0%, and the average daily average humidity was 70.53% in the third quarter; and
Hydroponics method of tissue cultured taro, characterized in that the average daily minimum humidity of the fourth quarter is 27.4%, the average daily maximum humidity is 90.1%, and the average daily average humidity is 74.97%; According to claim 1,
The step (S3) is divided into 4 quarters to vary the EC (Electrical Conductance, electrical conductivity) of the nutrient solution,
1Q average EC of 1.91 ds/m;
Q2 average EC of 1.57 ds/m;
Average EC in Q3 was 1.63 ds/m; and
A hydroponic cultivation method of tissue cultured taro, characterized in that the average EC of the fourth quarter is 0.96 ds/m; According to claim 1,
The nutrient solution of step (S3) is nitrogen (N) 5.8 me/L, phosphorus (P) 1.7 me/L, potassium (K) 3.4 me/L, calcium (Ca) 2.2 me/L, magnesium (Mg) 1.1 me /L and sulfur (S) 1.2 me / L of tissue cultured taro hydroponics, characterized in that it contains. According to claim 1,
The step (S3) is divided into 4 quarters to vary the daily average cumulative insolation,
The average daily cumulative solar insolation in the first quarter is 1061.25 J/cm ² ;
The average daily insolation in the second quarter was 680.76 J/cm ² ;
The average daily cumulative insolation in the third quarter was 662.19 J/cm ² ; and
A hydroponic cultivation method of tissue cultured taro, characterized in that the average daily average insolation in the fourth quarter is 641.15 J/cm ² ;

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