CN103918557A - Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro - Google Patents
Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro Download PDFInfo
- Publication number
- CN103918557A CN103918557A CN201410168948.8A CN201410168948A CN103918557A CN 103918557 A CN103918557 A CN 103918557A CN 201410168948 A CN201410168948 A CN 201410168948A CN 103918557 A CN103918557 A CN 103918557A
- Authority
- CN
- China
- Prior art keywords
- test
- shelf
- transplanting
- tube plantlets
- skin box
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Abstract
The invention relates to a method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro. The method comprises the following steps: (1) preparing an aluminum alloy transplanting and domesticating rack; (2) carrying out rooting culture on the test-tube plantlets of the red-bud taro for 30-60 days, directly taking out the test-tube plantlets subjected to rooting culture to put in a concrete poured plantlet washing pool, and washing the test-tube plantlets with tap water; (3) putting a layer of cunninghamia wood shaving through dry heat sterilization in an aluminum skin box on the transplanting and domesticating rack; (4) putting the washed test-tube plantlets on the wood shaving in multiple rows; (5) paving a layer of wood shaving again at the roots of the test-tube plantlets subjected to high-temperature high-pressure sterilization, and exposing stem leaves; (6) setting the illumination time of an LED (Light-Emitting Diode) lamp belt to be 12h/d, spraying tap water added with urine to the test-tube plantlets with a porous watering pot every morning and night, and taking out the test-tube plantlets after young leaves grow out, and transplanting into fields, wherein the rate of survival is 100%. Compared with the prior art, the method has the advantages that the rate of survival for transplanting the test-tube plantlets of the red-bud taro is high, the cost is saved, and the operation is convenient.
Description
Technical field:
The present invention relates to a Plants hardening off method, is exactly a kind of method of Qianshan, Jiangxi taro with red buds test-tube seedling transplanting domestication.
Background technology:
Taro with red buds is Qianshan County, Jiangxi Province traditional advantage product, plants with a long history.In the recent decade, this strong industry development rapidly, become the leading industry that Qianshan County increasing peasant income are got rich, Qianshan County also becomes the taro with red buds production base of Jiangxi Province's maximum, and in March, 2007 is by the China Green Food committee and Ministry of Agriculture's called after " green food standard production base county, the taro with red buds whole nation ".Yet, because the long-term vegetative propagation on producing causes virus day by day to accumulate, plant sexual involution, output, quality decline year after year, to taro with red buds, produce and cause greater loss.At present, utilize the test-tube plantlet that taro with red buds stem apex is cultivated to solve taro with red buds seedling problem in short supply.But in practice, we find, it is loaded down with trivial details that taro with red buds detoxic seedling is transplanted domestication program comparision, and the oversize bad transplanting of words of test tube shoot root, need to cut root, the survival rate that causes moving into land for growing field crops is not high.Therefore, how to simplify the program of taro with red buds test-tube seedling transplanting domestication, improve its survival rate, significant for the suitability for industrialized production that further realizes Qianshan, Jiangxi taro with red buds test-tube plantlet.
Summary of the invention:
The method that the object of this invention is to provide Qianshan, the Jiangxi taro with red buds test-tube seedling transplanting domestication that a kind of simple and convenient cost is low.The technical scheme that realizes the object of the invention is, the method of Qianshan, a kind of Jiangxi taro with red buds test-tube seedling transplanting domestication, it is characterized in that there are following steps: (1) prepares to transplant domestication frame: the adjustable shelf of aluminium alloy assembling, shelf length and width and shelf quantity are determined according to plant area, shelf height is determined according to factory building height, four pillars of shelf respectively fill a flexible LED white light band (60-80 pearl/m, lamp strip length equates with pillar height or is slightly short), the every interlamellar spacing of shelf is 30-40cm, every layer of uncovered aluminium skin box that is equipped with a bear (can be customized, length and width are consistent with the length and width of every layer of aluminium alloy adjustable shelf, high 5-6cm, the diameter in hole is 0.5cm), the bottom of each shelf is equipped with a uncovered aluminium skin box not punching in onesize bottom, be convenient to collect the moisture of every layer of seepage of shelf, (2) taro with red buds simple bud is in the liquid nutrient medium of the wonderful L of MS+NAA0.5m after culture of rootage 30-60d, open bottle cap, with tweezers, take out test-tube plantlet, directly put into the Xi Miaochi that cement is built, inject gently running water, with have gentle hands, gently stir after 1-3 time, with hand, pull out, put into tool hole fruit frame standby, note not hindering root leaf, (3) at place, open vacant lot, the China fir wood shavings (size is about 1cm * 1cm) after autoclave sterilization is put in aluminium skin box, placed thickness and be about 2cm, (4) by test-tube plantlet washed in fruit frame, rhizome, towards unanimously, is neatly placed on the wood shavings in aluminium skin box, can put test-tube plantlet and count row in each aluminium skin box, and every line space is 3-4cm, (5) at the test-tube plantlet root setting, repave the wood shavings (autoclave sterilization) that the about 0.5-1cm of one deck is thick, root and bulb are covered, but will notice that cauline leaf exposes, finally aluminium skin box is put on the cross bar of transplanting every layer, domestication frame, (6) flexible LED white light band connects 220v power supply by microcomputer time-controlled switch, every day, light application time was 12h, temperature is Indoor Natural temperature, notice that the winter closes the ventilation of windowing of warming summer of window, with pore watering can, to test-tube plantlet, spray the running water (urine: water=1: 10) that adds urine every day, sooner or later respectively once, it is wet that wood shavings becomes, the bottom uncovered atresia aluminium skin box of placing of shelf is collected the moisture of every layer of seepage, can refund pore watering can recycles, after seedling grows young leaves, can bring out aluminium skin box to land for growing field crops open place, test-tube plantlet is taken out gently, in order to avoid injury radicula, the wood shavings in small, broken bits that root sticks is powerful removing not, move into together land for growing field crops, survival rate is 100%.Compared with the prior art, the survival rate after taro with red buds test-tube seedling transplanting is high, and cost-saving, easy to operate in the present invention, can realize the batch production operation of Qianshan, Jiangxi taro with red buds test-tube seedling transplanting domestication.
Embodiment:
In conjunction with following embodiment, the invention will be further described:
(1) prepare to transplant domestication frame
The adjustable shelf of aluminium alloy assembling, shelf length and width and shelf quantity are determined according to plant area, shelf height is determined according to factory building height, four pillars of shelf respectively fill a flexible LED white light band (60-80 pearl/m, lamp strip length equates with pillar height or is slightly short), the every interlamellar spacing of shelf is 30-40cm, every layer of uncovered aluminium skin box that is equipped with a bear (can be customized, length and width are consistent with the length and width of every layer of aluminium alloy adjustable shelf, high 5-6cm, the diameter in hole is 0.5cm), the bottom of each shelf is equipped with a uncovered aluminium skin box not punching in onesize bottom, be convenient to collect the moisture of every layer of seepage of shelf,
(2) wash seedling
Taro with red buds simple bud is in the liquid nutrient medium of MS+NAA0.5mg/L after culture of rootage 30-60d, open bottle cap, with tweezers, take out test-tube plantlet, directly put into the Xi Miaochi that cement is built, inject gently running water, with have gentle hands, gently stir after 1-3 time, with hand, pull out, put into tool hole fruit frame standby, note not hindering root leaf;
(3) in aluminium skin box, place China fir wood shavings
At place, open vacant lot, the China fir wood shavings (size is about 1cm * 1em) after autoclave sterilization is put in aluminium skin box, place thickness and be about 2cm;
(4) pendulum seedling
By test-tube plantlet washed in fruit frame, rhizome, towards unanimously, is neatly placed on the wood shavings in aluminium skin box, can put test-tube plantlet and count row in each aluminium skin box, and every line space is 3-4cm;
(5) shoot root portion repaves one deck wood shavings
At the test-tube plantlet root setting, repave the wood shavings (autoclave sterilization) that the about 0.5-1cm of one deck is thick, root and bulb are covered, but will notice that cauline leaf exposes, finally aluminium skin box is put on the cross bar of transplanting every layer, domestication frame;
(6) hardening
Flexible LED white light band connects 220v power supply by microcomputer time-controlled switch, every day, light application time was 12h, temperature is Indoor Natural temperature, notice that the winter closes the ventilation of windowing of warming summer of window, with pore watering can, to test-tube plantlet, spray the running water (urine: water=1: 10) that adds urine every day, sooner or later respectively once, it is wet that wood shavings becomes, the bottom uncovered atresia aluminium skin box of placing of shelf is collected the moisture of every layer of seepage, can refund pore watering can recycles, after seedling grows young leaves, can bring out aluminium skin box to land for growing field crops open place, test-tube plantlet is taken out gently, in order to avoid injury radicula, the wood shavings in small, broken bits that root sticks is powerful removing not, move into together land for growing field crops, survival rate is 100%.
Claims (1)
1. the method that taro with red buds test-tube seedling transplanting in Qianshan, Jiangxi is tamed, it is characterized in that there are following steps: (1) prepares to transplant domestication frame: the adjustable shelf of aluminium alloy assembling, shelf length and width and shelf quantity are determined according to plant area, shelf height is determined according to factory building height, four pillars of shelf respectively fill a flexible LED white light band (lamp strip length equates with pillar height or be slightly short), the every interlamellar spacing of shelf is 30-40cm, every layer of uncovered aluminium skin box that is equipped with a bear (can be customized, length and width are consistent with the length and width of every layer of aluminium alloy adjustable shelf, high 5-6cm, the diameter in hole is 0.5cm), the bottom of each shelf is equipped with a uncovered aluminium skin box not punching in onesize bottom, be convenient to collect the moisture of every layer of seepage of shelf, (2) taro with red buds simple bud is cultivated after 30-60d in liquid root media, takes out test-tube plantlet, directly puts into the Xi Miaochi that cement is built, inject gently running water, with have gentle hands, gently stir after 1-3 time, with hand, pull out, put into tool hole fruit frame standby, note not hindering root leaf, (3) at place, open vacant lot, the China fir wood shavings (size is about 1cm * 1em) after autoclave sterilization is put in aluminium skin box, placed thickness and be about 2cm, (4) by test-tube plantlet washed in fruit frame, rhizome, towards unanimously, is neatly placed on the wood shavings in aluminium skin box, can put test-tube plantlet and count row in each aluminium skin box, and every line space is 3-4cm, (5) at the test-tube plantlet root setting, repave the wood shavings (autoclave sterilization) that the about 0.5-1cm of one deck is thick, root and bulb are covered, but will notice that cauline leaf exposes, finally aluminium skin box is put on the cross bar of transplanting every layer, domestication frame, (6) flexible LED white light band connects 220v power supply by microcomputer time-controlled switch, every day, light application time was 12h, temperature is chamber 7 natural temperatures, notice that the winter closes the ventilation of windowing of warming summer of window, with pore watering can, to test-tube plantlet, spray the running water (urine: water=1: 10) that adds urine every day, sooner or later respectively once, it is wet that wood shavings becomes, the bottom uncovered atresia aluminium skin box of placing of shelf is collected the moisture of every layer of seepage, can refund pore watering can recycles, after seedling grows young leaves, can bring out aluminium skin box to land for growing field crops open place, test-tube plantlet is taken out gently, in order to avoid injury radicula, the wood shavings in small, broken bits that root sticks is powerful removing not, move into together land for growing field crops, survival rate is 100%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168948.8A CN103918557A (en) | 2014-04-18 | 2014-04-18 | Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410168948.8A CN103918557A (en) | 2014-04-18 | 2014-04-18 | Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103918557A true CN103918557A (en) | 2014-07-16 |
Family
ID=51137147
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410168948.8A Pending CN103918557A (en) | 2014-04-18 | 2014-04-18 | Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103918557A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN104186148A (en) * | 2014-08-18 | 2014-12-10 | 江西省江天农业科技有限公司 | Red bud taro planting method for delayed harvest |
| CN112655561A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for in vitro preservation of red bud taro test-tube plantlet |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000093031A (en) * | 1998-09-29 | 2000-04-04 | Kubota Corp | Growth of araceous plant |
| JP2000350525A (en) * | 1999-06-10 | 2000-12-19 | Yamagata Prefecture | Growth and rearing of taro seedling for setting |
| CN102217550A (en) * | 2011-06-09 | 2011-10-19 | 马宗新 | Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
-
2014
- 2014-04-18 CN CN201410168948.8A patent/CN103918557A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000093031A (en) * | 1998-09-29 | 2000-04-04 | Kubota Corp | Growth of araceous plant |
| JP2000350525A (en) * | 1999-06-10 | 2000-12-19 | Yamagata Prefecture | Growth and rearing of taro seedling for setting |
| CN102217550A (en) * | 2011-06-09 | 2011-10-19 | 马宗新 | Fast-propagation technology and composition of culture medium for virus-free plantlets of red bud taros |
| CN102986532A (en) * | 2012-11-10 | 2013-03-27 | 上饶师范学院 | Red-bud taro micro tuber callus induction, differentiation and plant regeneration method |
Non-Patent Citations (2)
| Title |
|---|
| HONG SEN-RONG ET.AL.,: "A simple cryopreservation protocol for in vitro-grown shoot tips of Chinese genuine red bud taro (Colocasia esculenta L. Schott Var. Cormosus CV. Hongyayu) by encapsulation-dehydration", 《SCIENTIA HORTICULTURAE》, vol. 162, 31 December 2013 (2013-12-31), pages 226 - 233, XP028748004, DOI: 10.1016/j.scienta.2013.08.008 * |
| 洪森荣等: "江西铅山红芽芋脱毒苗球茎愈伤组织诱导及其再生体系的建立", 《植物研究》, vol. 33, no. 6, 31 December 2013 (2013-12-31) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104145680A (en) * | 2014-08-18 | 2014-11-19 | 江西省江天农业科技有限公司 | Method for propagating detoxification colocasia konishii hayata seeds |
| CN104186148A (en) * | 2014-08-18 | 2014-12-10 | 江西省江天农业科技有限公司 | Red bud taro planting method for delayed harvest |
| CN112655561A (en) * | 2021-01-14 | 2021-04-16 | 上饶师范学院 | Method for in vitro preservation of red bud taro test-tube plantlet |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103782686B (en) | A kind of watermelon plantlet in vitro grafting method | |
| CN103931497B (en) | A kind of method improving dragon fruit plantlet in vitro planting percent | |
| CN103190343B (en) | Key technology of organic additive for roxburgh anoectochilus terminal bud industrialization intermediate propagation | |
| CN103371093A (en) | Azalea tissue culture seedling out-bottle rooting method | |
| CN104082148A (en) | Method for performing regeneration propagation on sterile stalks of butterfly orchids | |
| CN103583220A (en) | Method for cultivating greenhouse Chinese toon without dormancy | |
| CN104521751A (en) | Tissue culture efficient breeding and seedling growing method of hybrid orchid Huangjinxiaoshentong | |
| CN102612889A (en) | Method for increasing seedling survival rate of Lonicera sempervirens seeds | |
| CN103718935A (en) | Greenhouse cultivation method for butterfly orchid | |
| CN106332777A (en) | Tissue culture method of acer rubrum | |
| CN104982285B (en) | Based on the Banana Seedlings rapid propagation method for inhaling bud | |
| CN103430842A (en) | Quick propagation method of hybrid orchid tissue culture | |
| CN103004604B (en) | Breeding method for vanda | |
| CN103918557A (en) | Method for transplanting and domesticating test-tube plantlets of Jiangxi Qianshan red-bud taro | |
| CN106305075A (en) | Narcissus flower culture method | |
| CN102524012B (en) | Exercising and transplanting method for fruit tree tissue culture seedlings | |
| CN103404432B (en) | The tissue culture and rapid proliferation method of poinsettia | |
| CN112042540A (en) | Rapid propagation method of hydrangea macrophylla | |
| CN104885943A (en) | Chemical disinfection tissue culture method for oncidium hybridum | |
| CN102726302B (en) | Dark culture method for banana tissue culture | |
| CN104620820A (en) | Method for preparing etiolated seedling using plant branch | |
| CN105660391A (en) | Tissue culture breeding method for apple sapling | |
| CN104488721B (en) | A kind of quick breeding method for tissue culture of snowflake grass | |
| CN103609452A (en) | Tissue-culture rapid propagation method of cymbidium | |
| CN101707962B (en) | Method for cultivating tree-rose semi-finished rose trees through U.S.A. M1 and M2 rootstocks |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140716 |