IN2014DN06516A - - Google Patents
Info
- Publication number
- IN2014DN06516A IN2014DN06516A IN6516DEN2014A IN2014DN06516A IN 2014DN06516 A IN2014DN06516 A IN 2014DN06516A IN 6516DEN2014 A IN6516DEN2014 A IN 6516DEN2014A IN 2014DN06516 A IN2014DN06516 A IN 2014DN06516A
- Authority
- IN
- India
- Prior art keywords
- dna polymerase
- sample matrix
- microorganism
- amount
- selected suitable
- Prior art date
Links
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 abstract 4
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 abstract 4
- 239000011159 matrix material Substances 0.000 abstract 3
- 239000000523 sample Substances 0.000 abstract 3
- 238000003556 assay Methods 0.000 abstract 2
- 238000001514 detection method Methods 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 abstract 1
- 230000001351 cycling effect Effects 0.000 abstract 1
- 238000005259 measurement Methods 0.000 abstract 1
- 239000002853 nucleic acid probe Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2521/00—Reaction characterised by the enzymatic activity
- C12Q2521/10—Nucleotidyl transfering
- C12Q2521/101—DNA polymerase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicinal Chemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Pathology (AREA)
- General Physics & Mathematics (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261583568P | 2012-01-05 | 2012-01-05 | |
| PCT/US2013/020180 WO2013103744A1 (en) | 2012-01-05 | 2013-01-03 | Improved dna polymerase activity assays and methods enabling detection of viable microbes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2014DN06516A true IN2014DN06516A (cg-RX-API-DMAC10.html) | 2015-06-12 |
Family
ID=48745412
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN6516DEN2014 IN2014DN06516A (cg-RX-API-DMAC10.html) | 2012-01-05 | 2013-01-03 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US9708651B2 (cg-RX-API-DMAC10.html) |
| EP (1) | EP2800822B1 (cg-RX-API-DMAC10.html) |
| JP (1) | JP6388540B2 (cg-RX-API-DMAC10.html) |
| KR (1) | KR20150005511A (cg-RX-API-DMAC10.html) |
| CN (1) | CN104066853B (cg-RX-API-DMAC10.html) |
| AU (1) | AU2013206888A1 (cg-RX-API-DMAC10.html) |
| CA (1) | CA2897010C (cg-RX-API-DMAC10.html) |
| ES (1) | ES2624871T3 (cg-RX-API-DMAC10.html) |
| IN (1) | IN2014DN06516A (cg-RX-API-DMAC10.html) |
| WO (1) | WO2013103744A1 (cg-RX-API-DMAC10.html) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201412316D0 (en) * | 2014-07-10 | 2014-08-27 | Momentum Bioscience Ltd | Detecting the absence of micro-organisms |
| CN107541508A (zh) * | 2016-06-24 | 2018-01-05 | 广州康昕瑞基因健康科技有限公司 | 模板‑引物核酸分子、聚合酶活性测定方法及试剂盒 |
| CN106987643A (zh) * | 2017-05-05 | 2017-07-28 | 广州和实生物技术有限公司 | 一种 Taq DNA 聚合酶活性检测方法 |
| GB201805479D0 (en) | 2018-04-03 | 2018-05-16 | Momentum Bioscience Ltd | Microorganism separation and detection |
| CN109609606B (zh) * | 2018-11-28 | 2023-03-31 | 成都博奥晶芯生物科技有限公司 | 一种dna聚合酶的相对酶活的测定方法 |
| GB201914538D0 (en) | 2019-10-08 | 2019-11-20 | Momentum Bioscience Ltd | Microorganism capture from antimicrobial-containing solution |
| KR102441122B1 (ko) * | 2020-05-11 | 2022-09-07 | 주식회사 엔지노믹스 | 폴리머라제 활성 측정 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040005555A1 (en) * | 2000-08-31 | 2004-01-08 | Rothman Richard E. | Molecular diagnosis of bactermia |
| EP2418018B1 (en) * | 2004-12-23 | 2013-05-22 | Abbott Point of Care Inc. | Methods for the separation nucleic acids |
| GB0513535D0 (en) | 2005-07-01 | 2005-08-10 | Iseao Technologies Ltd | Improved methods of detecting microbes |
| US20080113349A1 (en) * | 2006-11-03 | 2008-05-15 | Pranvera Ikonomi | Method for detecting the presence of mammalian organisms using specific cytochrome c oxidase I (COI) and/or cytochrome b subsequences by a PCR based assay |
| CN101849019A (zh) | 2007-07-09 | 2010-09-29 | 微敏医疗技术有限公司 | 基于其nad依赖性dna连接酶活性来检测微生物 |
| JP2011188855A (ja) * | 2010-03-11 | 2011-09-29 | Samsung Techwin Co Ltd | 切断可能なキメラプローブを使用したサルモネラのリアルタイム検出方法 |
| MY161567A (en) * | 2010-04-16 | 2017-04-28 | Zeus Scientific Inc | Methods for measuring enzyme activity useful in determining cell viability in non-purified samples |
| CN104520438A (zh) * | 2012-04-12 | 2015-04-15 | 宙斯科技公司 | 用于任何聚合酶延伸活性的灵敏的,定量的聚合酶活性检测和用于确定活细胞的存在的方法 |
-
2013
- 2013-01-03 CA CA2897010A patent/CA2897010C/en active Active
- 2013-01-03 AU AU2013206888A patent/AU2013206888A1/en not_active Abandoned
- 2013-01-03 WO PCT/US2013/020180 patent/WO2013103744A1/en not_active Ceased
- 2013-01-03 ES ES13733757.2T patent/ES2624871T3/es active Active
- 2013-01-03 JP JP2014551321A patent/JP6388540B2/ja active Active
- 2013-01-03 US US14/370,752 patent/US9708651B2/en active Active
- 2013-01-03 KR KR1020147021867A patent/KR20150005511A/ko not_active Ceased
- 2013-01-03 EP EP13733757.2A patent/EP2800822B1/en active Active
- 2013-01-03 CN CN201380004900.7A patent/CN104066853B/zh active Active
- 2013-01-03 IN IN6516DEN2014 patent/IN2014DN06516A/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| ES2624871T3 (es) | 2017-07-17 |
| CN104066853A (zh) | 2014-09-24 |
| CA2897010C (en) | 2017-11-07 |
| WO2013103744A1 (en) | 2013-07-11 |
| US20150004617A1 (en) | 2015-01-01 |
| CA2897010A1 (en) | 2013-07-11 |
| CN104066853B (zh) | 2019-05-31 |
| AU2013206888A1 (en) | 2014-08-21 |
| EP2800822B1 (en) | 2017-03-29 |
| EP2800822A4 (en) | 2015-08-26 |
| KR20150005511A (ko) | 2015-01-14 |
| EP2800822A1 (en) | 2014-11-12 |
| JP6388540B2 (ja) | 2018-09-12 |
| US9708651B2 (en) | 2017-07-18 |
| JP2015503925A (ja) | 2015-02-05 |
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