IN2014DN01848A - - Google Patents

Info

Publication number
IN2014DN01848A
IN2014DN01848A IN1848DEN2014A IN2014DN01848A IN 2014DN01848 A IN2014DN01848 A IN 2014DN01848A IN 1848DEN2014 A IN1848DEN2014 A IN 1848DEN2014A IN 2014DN01848 A IN2014DN01848 A IN 2014DN01848A
Authority
IN
India
Prior art keywords
sample
biosample
thin film
electron microscope
electron
Prior art date
Application number
Other languages
English (en)
Inventor
Takahiko Hariyama
Yasuharu TAKAKU
Hiroshi Suzuki
Yoshinori Muranaka
Isao Ohta
Masatsugu Shimomura
Daisuke Ishii
Original Assignee
Japan Science & Tech Agency
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Science & Tech Agency filed Critical Japan Science & Tech Agency
Publication of IN2014DN01848A publication Critical patent/IN2014DN01848A/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/225Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
    • G01N23/2251Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion using incident electron beams, e.g. scanning electron microscopy [SEM]
    • G01N23/2252Measuring emitted X-rays, e.g. electron probe microanalysis [EPMA]
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/02Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
    • G01N23/04Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2853Shadowing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/44Sample treatment involving radiation, e.g. heat
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N23/00Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
    • G01N23/22Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material
    • G01N23/225Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by measuring secondary emission from the material using electron or ion
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/02Details
    • H01J37/20Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J37/00Discharge tubes with provision for introducing objects or material to be exposed to the discharge, e.g. for the purpose of examination or processing thereof
    • H01J37/26Electron or ion microscopes; Electron or ion diffraction tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2223/00Investigating materials by wave or particle radiation
    • G01N2223/60Specific applications or type of materials
    • G01N2223/612Specific applications or type of materials biological material
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/20Positioning, supporting, modifying or maintaining the physical state of objects being observed or treated
    • H01J2237/2002Controlling environment of sample
    • HELECTRICITY
    • H01ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J2237/00Discharge tubes exposing object to beam, e.g. for analysis treatment, etching, imaging
    • H01J2237/20Positioning, supporting, modifying or maintaining the physical state of objects being observed or treated
    • H01J2237/2002Controlling environment of sample
    • H01J2237/2003Environmental cells
    • H01J2237/2004Biological samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Sampling And Sample Adjustment (AREA)
IN1848DEN2014 2011-09-09 2012-09-07 IN2014DN01848A (enrdf_load_html_response)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2011197685 2011-09-09
JP2012044383 2012-02-29
PCT/JP2012/072982 WO2013035866A1 (ja) 2011-09-09 2012-09-07 生物試料をそのままの姿で観察するための電子顕微鏡による観察方法とそれに用いられる真空下での蒸発抑制用組成物、走査型電子顕微鏡および透過型電子顕微鏡

Publications (1)

Publication Number Publication Date
IN2014DN01848A true IN2014DN01848A (enrdf_load_html_response) 2015-05-15

Family

ID=47832305

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1848DEN2014 IN2014DN01848A (enrdf_load_html_response) 2011-09-09 2012-09-07

Country Status (7)

Country Link
US (1) US9557253B2 (enrdf_load_html_response)
EP (1) EP2755225B1 (enrdf_load_html_response)
JP (1) JP6055766B2 (enrdf_load_html_response)
KR (1) KR101650054B1 (enrdf_load_html_response)
CN (1) CN103843106B (enrdf_load_html_response)
IN (1) IN2014DN01848A (enrdf_load_html_response)
WO (1) WO2013035866A1 (enrdf_load_html_response)

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JP5922908B2 (ja) * 2011-10-27 2016-05-24 富士フイルム株式会社 硬化物の製造方法および硬化物
US10128078B2 (en) 2014-01-29 2018-11-13 Japan Science And Technology Agency Protective agent for electron microscopic observation of biological sample in water-containing state, kit for electron microscopic observation, methods for observation, diagnosis, evaluation, and quantification by electron microscope, and sample stage
JP6496184B2 (ja) * 2014-06-27 2019-04-03 日本電子株式会社 試料導入方法、試料ステージ、および荷電粒子線装置
KR101792832B1 (ko) * 2014-10-29 2017-11-20 충남대학교산학협력단 기-액 계면 플라즈마 중합에 의한 고분자 박막의 제조방법 및 이에 의해 제조된 고분자 박막
JP6569151B2 (ja) * 2015-02-25 2019-09-04 学校法人日本大学 イオン液体を用いた透過型電子顕微鏡による錯体の観察方法及び観察用試料
CN104908063A (zh) * 2015-06-24 2015-09-16 中山安荞生物科技有限公司 一种真空手套箱
DE102016202379A1 (de) * 2016-02-17 2017-08-17 Carl Zeiss Microscopy Gmbh Verfahren zur Präparation von Objekten für Untersuchungen mit geladenen Teilchenstrahlen
TWI594288B (zh) * 2016-03-14 2017-08-01 台灣電鏡儀器股份有限公司 電子顯微鏡
EP3509651B1 (en) 2016-09-06 2022-12-28 Miromatrix Medical Inc. Use of resected liver serum for whole liver engineering
JP2018078028A (ja) * 2016-11-09 2018-05-17 株式会社メルビル 試料ホルダー
CN107860783B (zh) * 2017-10-23 2019-09-24 中国科学院化学研究所 Xps进样装置
JP7001425B2 (ja) * 2017-10-26 2022-01-19 シンフォニアテクノロジー株式会社 マウンタ用エア制御装置
EP3737927B1 (en) * 2018-01-10 2023-09-20 University Of Kansas Conductive fixation for electron microscopy
CN110243640B (zh) * 2018-03-08 2022-01-25 中国科学院海洋研究所 深海有孔虫的内腔磨片快速制备方法
CN108802080A (zh) * 2018-08-16 2018-11-13 扬州大学 一种玉米成熟种子中胚乳淀粉生长环的原位观察方法
AU2020241583A1 (en) * 2019-03-21 2021-10-14 Miromatrix Medical Inc. Improved decellularization of isolated organs
CN109916942B (zh) * 2019-04-18 2021-09-21 攀钢集团攀枝花钢铁研究院有限公司 捕收剂的电子显微镜表征方法
CN110441084A (zh) * 2019-09-04 2019-11-12 海南省农业科学院热带果树研究所 一种菠萝蜜果肉的冰冻切片方法
WO2021070338A1 (ja) * 2019-10-10 2021-04-15 株式会社日立ハイテク 荷電粒子線装置
WO2021256412A1 (ja) 2020-06-16 2021-12-23 学校法人中部大学 走査型電子顕微鏡を用いた観察方法、及びそのための試料ホルダ
CN111855507A (zh) * 2020-07-17 2020-10-30 北方民族大学 一种空气颗粒物的检测装置及检测方法
JP7665174B2 (ja) * 2020-09-26 2025-04-21 NanoSuit株式会社 食品のコーティング方法及びコーティング装置
CN112611775B (zh) * 2020-12-16 2023-08-11 聚束科技(北京)有限公司 一种用于观察生物组织的方法及电子显微镜
KR102740532B1 (ko) 2021-11-03 2024-12-16 대한민국 스마트 온실 로봇을 위한 농약 자동 공급 장치 및 그 동작 방법
JP7685158B2 (ja) * 2022-01-28 2025-05-30 国立大学法人千葉大学 被覆装置、被覆方法
CN114773216A (zh) * 2022-03-24 2022-07-22 天津大学 一种l-亮氨酸球形晶体及其制备方法和应用
CN116396809A (zh) * 2023-03-30 2023-07-07 橙的(福建)卫生用品有限责任公司 一种厨房湿巾的制备方法
CN118330207B (zh) * 2024-06-12 2024-08-09 成都海默云因医学检验实验室有限公司 一种改善固定细胞免疫荧光法中荧光背景的固定剂及其固定细胞的方法

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Also Published As

Publication number Publication date
CN103843106B (zh) 2016-12-07
KR20140074287A (ko) 2014-06-17
US20140227734A1 (en) 2014-08-14
US9557253B2 (en) 2017-01-31
EP2755225A1 (en) 2014-07-16
CN103843106A (zh) 2014-06-04
EP2755225A4 (en) 2015-08-05
WO2013035866A1 (ja) 2013-03-14
JPWO2013035866A1 (ja) 2015-03-23
KR101650054B1 (ko) 2016-08-22
JP6055766B2 (ja) 2016-12-27
EP2755225B1 (en) 2017-05-10

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