IN2012DN02396A - - Google Patents

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Publication number
IN2012DN02396A
IN2012DN02396A IN2396DEN2012A IN2012DN02396A IN 2012DN02396 A IN2012DN02396 A IN 2012DN02396A IN 2396DEN2012 A IN2396DEN2012 A IN 2396DEN2012A IN 2012DN02396 A IN2012DN02396 A IN 2012DN02396A
Authority
IN
India
Prior art keywords
isobutanol
cells
isolation
observed
developed
Prior art date
Application number
Other languages
English (en)
Inventor
Brian James Paul
Wonchul Suh
Original Assignee
Butamax Tm Advanced Biofuels
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Butamax Tm Advanced Biofuels filed Critical Butamax Tm Advanced Biofuels
Publication of IN2012DN02396A publication Critical patent/IN2012DN02396A/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/16Butanols
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0006Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y101/00Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01027L-Lactate dehydrogenase (1.1.1.27)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/01054Formate C-acetyltransferase (2.3.1.54), i.e. pyruvate formate-lyase or PFL
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y401/00Carbon-carbon lyases (4.1)
    • C12Y401/01Carboxy-lyases (4.1.1)
    • C12Y401/01005Acetolactate decarboxylase (4.1.1.5)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
IN2396DEN2012 2009-09-29 2010-09-29 IN2012DN02396A (enrdf_load_stackoverflow)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24671709P 2009-09-29 2009-09-29
PCT/US2010/050705 WO2011041402A1 (en) 2009-09-29 2010-09-29 Improved flux to acetolactate-derived products in lactic acid bacteria

Publications (1)

Publication Number Publication Date
IN2012DN02396A true IN2012DN02396A (enrdf_load_stackoverflow) 2015-08-21

Family

ID=43093547

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2396DEN2012 IN2012DN02396A (enrdf_load_stackoverflow) 2009-09-29 2010-09-29

Country Status (9)

Country Link
US (1) US9169499B2 (enrdf_load_stackoverflow)
EP (1) EP2483400A1 (enrdf_load_stackoverflow)
JP (1) JP2013505739A (enrdf_load_stackoverflow)
CN (1) CN102712912A (enrdf_load_stackoverflow)
AU (1) AU2010300722A1 (enrdf_load_stackoverflow)
BR (1) BR112012006941A2 (enrdf_load_stackoverflow)
CA (1) CA2776151A1 (enrdf_load_stackoverflow)
IN (1) IN2012DN02396A (enrdf_load_stackoverflow)
WO (1) WO2011041402A1 (enrdf_load_stackoverflow)

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EP2543721A1 (en) 2007-04-18 2013-01-09 Butamax (TM) Advanced Biofuels LLC Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
US8188250B2 (en) 2008-04-28 2012-05-29 Butamax(Tm) Advanced Biofuels Llc Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
CA2735945A1 (en) 2008-09-29 2010-04-01 Butamaxtm Advanced Biofuels Llc Increased heterologous fe-s enzyme activity in yeast
WO2011041426A1 (en) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Improved yeast production host cells
BR112012016042A2 (pt) 2009-12-29 2020-09-15 Butamax Advanced Biofuels Llc "celula hospedeira microbiana recombinante, metodo para a produção de isobutanol, método para a produção de 2-butanol e método para a produção de 14-butanol
EP2519629A1 (en) 2009-12-29 2012-11-07 Butamax (TM) Advanced Biofuels LLC Expression of hexose kinase in recombinant host cells
JP5950830B2 (ja) 2010-02-17 2016-07-13 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー Fe−Sクラスター要求タンパク質の活性の改善
MX346408B (es) 2010-09-07 2017-03-17 Butamax(Tm) Advanced Biofuels Llc Integracion de polinucleotido que codifica polipeptido que cataliza conversion de piruvato a acetolactato.
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BR112013027553A2 (pt) 2011-04-29 2019-09-24 Danisco Us Inc produção de mevalonato, isopreno, e isoprenoides com uso de genes que codificam polipeptídeos tendo atividades enzimáticas de tiolase, hmg-coa sintase e hmg-coa redutase
CN105473726B (zh) 2011-07-28 2021-03-12 布特马斯先进生物燃料有限责任公司 酮异戊酸脱羧酶及其使用方法
WO2013102084A2 (en) 2011-12-30 2013-07-04 Butamax (Tm) Advanced Biofuels Llc Fermentative production of alcohols
CN102559736B (zh) * 2012-02-24 2014-01-08 常茂生物化学工程股份有限公司 提高富马酸产量的基因工程菌株及构建方法及其应用
JP2015510774A (ja) 2012-03-23 2015-04-13 ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー ブタノール産生菌用培地の酢酸補給
MX2014013446A (es) 2012-05-11 2014-12-08 Butamax Advanced Biofuels Llc Enzimas de cetol-acido reductoisomerasa y metodos de uso.
CN102816787B (zh) * 2012-06-20 2015-05-06 江南大学 利用重组大肠杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
WO2014025604A2 (en) * 2012-08-07 2014-02-13 Gevo, Inc. Microorganisms for improved production of fuels, chemicals, and amino acids
AU2013305714A1 (en) 2012-08-22 2015-02-05 Butamax Advanced Biofuels Llc Production of fermentation products
CN102876704A (zh) * 2012-09-20 2013-01-16 江南大学 利用重组枯草芽孢杆菌高效表达Staphyloccocus aureus α-乙酰乳酸脱羧酶
WO2014047421A1 (en) 2012-09-21 2014-03-27 Butamax(Tm) Advanced Biofuels Llc Production of renewable hydrocarbon compositions
EP2900811B1 (en) 2012-09-26 2018-12-19 Butamax Advanced Biofuels LLC Polypeptides with ketol-acid reductoisomerase activity
US9273330B2 (en) 2012-10-03 2016-03-01 Butamax Advanced Biofuels Llc Butanol tolerance in microorganisms
US9650624B2 (en) 2012-12-28 2017-05-16 Butamax Advanced Biofuels Llc DHAD variants for butanol production
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WO2014144210A2 (en) 2013-03-15 2014-09-18 Butamax Advanced Biofuels Llc Competitive growth and/or production advantage for butanologen microorganism
US9580705B2 (en) 2013-03-15 2017-02-28 Butamax Advanced Biofuels Llc DHAD variants and methods of screening
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CN108570438B (zh) * 2017-03-08 2020-06-30 中国科学院上海高等研究院 提升克雷伯氏肺炎杆菌生产α-酮异戊酸的方法及改造菌
CN108486176B (zh) * 2018-05-18 2021-08-03 天津科技大学 一种产乳酸乙酯的酿酒酵母及其构建方法与应用
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Also Published As

Publication number Publication date
WO2011041402A1 (en) 2011-04-07
JP2013505739A (ja) 2013-02-21
CN102712912A (zh) 2012-10-03
CA2776151A1 (en) 2011-04-07
BR112012006941A2 (pt) 2015-09-08
US9169499B2 (en) 2015-10-27
AU2010300722A1 (en) 2012-04-12
EP2483400A1 (en) 2012-08-08
US20110136192A1 (en) 2011-06-09

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