WO2012016172A3 - Genetically modified microbes producing increased levels of acetyl-coa derived compounds - Google Patents

Genetically modified microbes producing increased levels of acetyl-coa derived compounds Download PDF

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Publication number
WO2012016172A3
WO2012016172A3 PCT/US2011/045947 US2011045947W WO2012016172A3 WO 2012016172 A3 WO2012016172 A3 WO 2012016172A3 US 2011045947 W US2011045947 W US 2011045947W WO 2012016172 A3 WO2012016172 A3 WO 2012016172A3
Authority
WO
WIPO (PCT)
Prior art keywords
acetyl
genetically modified
derived compounds
increased levels
coa derived
Prior art date
Application number
PCT/US2011/045947
Other languages
French (fr)
Other versions
WO2012016172A2 (en
Inventor
Darren Platt
Original Assignee
Amyris, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amyris, Inc. filed Critical Amyris, Inc.
Publication of WO2012016172A2 publication Critical patent/WO2012016172A2/en
Publication of WO2012016172A3 publication Critical patent/WO2012016172A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats

Abstract

Provided herein are genetically modified microorganisms that produce increased amounts of acetyl-CoA derived compounds in industrial fermentation processes, and methods for making and using the same.
PCT/US2011/045947 2010-07-30 2011-07-29 Genetically modified microbes producing increased levels of acetyl-coa derived compounds WO2012016172A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36954710P 2010-07-30 2010-07-30
US61/369,547 2010-07-30

Publications (2)

Publication Number Publication Date
WO2012016172A2 WO2012016172A2 (en) 2012-02-02
WO2012016172A3 true WO2012016172A3 (en) 2012-06-14

Family

ID=45530749

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/045947 WO2012016172A2 (en) 2010-07-30 2011-07-29 Genetically modified microbes producing increased levels of acetyl-coa derived compounds

Country Status (1)

Country Link
WO (1) WO2012016172A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170298363A1 (en) 2014-09-18 2017-10-19 Genomatica, Inc Non-natural microbial organisms with improved energetic efficiency
CN111699247A (en) 2017-12-07 2020-09-22 齐默尔根公司 Engineered biosynthetic pathway for the production of (6E) -8-hydroxygeraniol by fermentation
WO2019126778A1 (en) 2017-12-21 2019-06-27 Zymergen Inc. Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone

Non-Patent Citations (9)

* Cited by examiner, † Cited by third party
Title
"Production of Renewable Fuels and Chemicals in Genetically Engineered Saccharomyces cerevisiae.", BENJAMIN. POSTER 577B., July 2010 (2010-07-01), pages 220, Retrieved from the Internet <URL:http://www.yeast-meet.org/2010/pdf/book.pdf> [retrieved on 20120404] *
"UniProtKB/Swiss-Prot Direct Submission P38806.1.", CHROMATIN MODIFICATION-RELATED PROTEIN YNG2., 15 June 2010 (2010-06-15), Retrieved from the Internet <URL:http://www.ncbi.nlm.nih.gov/protein/731685?sat=138,satkey=55405> [retrieved on 20120404] *
LIN ET AL.: "A comprehensive synthetic genetic interaction network governing yeast histone acetylation and deacetylation.", GENES & DEV., vol. 22, no. 15, 2008, pages 2062 - 2074 *
NOURANI ET AL.: "Recruitment of the NuA4 complex poises the PH05 promoter for chromatin remodeling and activation.", EMBO J., vol. 23, no. 13, 2004, pages 2597 - 2607 *
NOURANI ET AL.: "Role of an ING1 growth regulator in transcriptional activation and targeted histone acetylation by the NuA4 complex.", MOL CELL BIOL., vol. 21, no. 22, 2001, pages 7629 - 7640 *
RO ET AL.: "Production of the antimalarial drug precursor artemisinic acid in engineered yeast.", NATURE, vol. 440, no. 7086, 2006, pages 940 - 943 *
SELLECK ET AL.: "Recombinant protein complex expression in E. coli.", CURR PROTOC PROTEIN SCI., 2008 *
SELLECK ET AL.: "The Saccharomyces cerevisiae Piccolo NuA4 histone acetyltransferase complex requires the Enhancer of Polycomb A domain and chromodomain to acetylate nucleosomes.", MOL CELL BIOL, vol. 25, 2005, pages 5535 - 5542 *
ZHOU ET AL.: "SWR1 Complex Poises Heterochromatin Boundaries for Antisilencing Activity Propagation.", MOLECULAR AND CELLULAR BIOLOGY, vol. 30, no. 10, May 2010 (2010-05-01), pages 2391 - 2400 *

Also Published As

Publication number Publication date
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