IN2012DN01992A - - Google Patents

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Publication number
IN2012DN01992A
IN2012DN01992A IN1992DEN2012A IN2012DN01992A IN 2012DN01992 A IN2012DN01992 A IN 2012DN01992A IN 1992DEN2012 A IN1992DEN2012 A IN 1992DEN2012A IN 2012DN01992 A IN2012DN01992 A IN 2012DN01992A
Authority
IN
India
Prior art keywords
nucleic acid
operably linked
acid sequence
reporter nucleic
expression vector
Prior art date
Application number
Other languages
English (en)
Inventor
Bhaumik Srabani
Sam Gambhir Sanjiv
Yaghoubi Shahriar
Parashurama Natesh
Ahn Byeong-Cheol
Paulmurugan Ramasamy
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of IN2012DN01992A publication Critical patent/IN2012DN01992A/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1086Preparation or screening of expression libraries, e.g. reporter assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/008Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/20Vector systems having a special element relevant for transcription transcription of more than one cistron
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00Vectors comprising a special translation-regulating system
    • C12N2840/20Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Chemical Kinetics & Catalysis (AREA)
IN1992DEN2012 2009-09-09 2010-09-06 IN2012DN01992A (he)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/555,901 US9719146B2 (en) 2009-09-09 2009-09-09 Composition and method for imaging stem cells
PCT/EP2010/063054 WO2011029798A1 (en) 2009-09-09 2010-09-06 Composition and method for imaging stem cells

Publications (1)

Publication Number Publication Date
IN2012DN01992A true IN2012DN01992A (he) 2015-07-24

Family

ID=43034378

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1992DEN2012 IN2012DN01992A (he) 2009-09-09 2010-09-06

Country Status (10)

Country Link
US (2) US9719146B2 (he)
EP (1) EP2475780B1 (he)
JP (1) JP6106436B2 (he)
KR (1) KR101760533B1 (he)
CN (1) CN102803508B (he)
AU (1) AU2010294316B2 (he)
CA (1) CA2773624C (he)
IN (1) IN2012DN01992A (he)
SG (1) SG179561A1 (he)
WO (1) WO2011029798A1 (he)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2551343B1 (en) * 2010-03-23 2018-05-02 Olympus Corporation Method for monitoring state of differentiation in a stem cell
US20130137604A1 (en) * 2011-11-30 2013-05-30 Raytheon Bbn Technologies Corp. Methods of evaluating genetic elements
WO2013142341A1 (en) * 2012-03-20 2013-09-26 The Regents Of The University Of California A novel rapid and highly sensitive cell based system for the detection and characterization of hiv
WO2013176233A1 (ja) * 2012-05-23 2013-11-28 国立大学法人京都大学 効率的な人工多能性幹細胞の樹立方法
JP6116226B2 (ja) * 2012-06-11 2017-04-19 オリンパス株式会社 幹細胞の状態を同定する方法
JP2014176364A (ja) * 2013-03-15 2014-09-25 Olympus Corp 幹細胞の解析方法
JP6223037B2 (ja) * 2013-07-22 2017-11-01 オリンパス株式会社 サバイビンについての細胞解析方法、並びにそれを利用した癌の解析方法および薬効のスクリーニング方法
JP6454478B2 (ja) 2013-09-12 2019-01-16 オリンパス株式会社 心筋細胞への分化をモニタリングする方法
EP3194623B1 (en) * 2014-08-12 2022-01-05 Wayne State University Systems and methods to detect stem cell stress and uses thereof
WO2018122932A1 (ja) * 2016-12-26 2018-07-05 オリンパス株式会社 細胞の選別方法、純化された細胞集団の製造方法、及び発光イメージングシステム
US11124847B1 (en) * 2017-10-03 2021-09-21 Nzumbe, Inc. Systems and methods for assessing perturbations in cellular differentiation using reporter cell lines
EP3976116A4 (en) * 2019-05-30 2023-08-23 490 Biotech, Inc. EXPRESSION OF BIOLUMINESCENCE IN CELLS AND METHODS OF USE
CN114164233A (zh) * 2021-11-05 2022-03-11 贵州医科大学附属医院 一种pProTG质粒、其构建方法及应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7008776B1 (en) * 1996-12-06 2006-03-07 Aventis Pharmaceuticals Inc. Compositions and methods for effecting the levels of high density lipoprotein (HDL) cholesterol and apolipoprotein AI very low density lipoprotein (VLDL) cholesterol and low density lipoprotein (LDL) cholesterol
DE19710643A1 (de) * 1997-03-14 1998-09-17 Hoechst Ag Der Promotor des cdc25B Genes und seine Verwendung in der Gentherapie
US6482937B1 (en) * 1997-10-09 2002-11-19 Biotransplant, Inc. Porcine Oct-4 promoter
KR20030032912A (ko) * 2000-01-14 2003-04-26 베쓰 이스라엘 디코니스 메디칼 센터 심장세포-특이적 인핸서 인자 및 이의 용도
WO2003018780A1 (en) * 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
US7723505B2 (en) * 2002-02-15 2010-05-25 The John Hopkins University EAAT2 promoter and uses thereof
US7399851B2 (en) * 2002-07-25 2008-07-15 Dana Farber Cancer Institute, Inc. Composition and method for imaging cells
US7524674B2 (en) * 2003-03-07 2009-04-28 Stanford University Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals
JP2006524051A (ja) 2003-04-24 2006-10-26 フォンダッチォーネ・セントロ・サン・ラファエル・デル・モンテ・タボール 両方向性合成プロモーターを備えたレンチウイルスベクターおよびその使用
US20050101017A1 (en) * 2003-11-10 2005-05-12 Wojtek Auerbach Method of improving gene targeting using a ubiquitin promoter
US7682828B2 (en) * 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
RU2006129469A (ru) * 2004-01-16 2008-02-27 Новартис АГ (CH) 2, 4-диаминопиримидины и их применение для индукции кардиомиогенеза
US8278104B2 (en) * 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
WO2007140617A1 (en) 2006-06-08 2007-12-13 Multi-Magnetics Incorporated Magnetosome gene expression in eukaryotic cells
US20080216185A1 (en) * 2007-01-19 2008-09-04 Invitrogen Corporation Compositions and Methods for Genetic Manipulation and Monitoring of Cell Lines
WO2008144580A2 (en) * 2007-05-17 2008-11-27 Oregon Health & Science University Primate stem cells produced by somatic cell nuclear transfer
WO2008144052A2 (en) 2007-05-18 2008-11-27 Rampyari Walia Bioluminescent imaging of stem cells
US20090137023A1 (en) * 2007-10-16 2009-05-28 Brandon John Casutt Cytoplasm of Eukaryotic cells for reprogramming of somatic cells, healing, repairing and therapeutic applications
KR100952368B1 (ko) 2007-11-29 2010-04-09 고려대학교 산학협력단 이중 프로모터 리포터유전자 발현 시스템 및 이를 이용한심근세포 및 혈관내피세포 분화를 동시에 모니터링하는방법

Also Published As

Publication number Publication date
EP2475780A1 (en) 2012-07-18
AU2010294316A1 (en) 2012-03-22
EP2475780B1 (en) 2015-03-04
WO2011029798A1 (en) 2011-03-17
JP6106436B2 (ja) 2017-03-29
US10920287B2 (en) 2021-02-16
US9719146B2 (en) 2017-08-01
CA2773624A1 (en) 2011-03-17
KR101760533B1 (ko) 2017-07-21
JP2013503648A (ja) 2013-02-04
SG179561A1 (en) 2012-05-30
US20170298455A1 (en) 2017-10-19
KR20120089667A (ko) 2012-08-13
US20110059439A1 (en) 2011-03-10
AU2010294316B2 (en) 2015-07-23
CN102803508B (zh) 2015-10-21
CN102803508A (zh) 2012-11-28
CA2773624C (en) 2019-07-30

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