IN2012DN01992A - - Google Patents

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Publication number
IN2012DN01992A
IN2012DN01992A IN1992DEN2012A IN2012DN01992A IN 2012DN01992 A IN2012DN01992 A IN 2012DN01992A IN 1992DEN2012 A IN1992DEN2012 A IN 1992DEN2012A IN 2012DN01992 A IN2012DN01992 A IN 2012DN01992A
Authority
IN
India
Prior art keywords
nucleic acid
operably linked
acid sequence
reporter nucleic
expression vector
Prior art date
Application number
Inventor
Bhaumik Srabani
Sam Gambhir Sanjiv
Yaghoubi Shahriar
Parashurama Natesh
Ahn Byeong-Cheol
Paulmurugan Ramasamy
Original Assignee
Gen Electric
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gen Electric filed Critical Gen Electric
Publication of IN2012DN01992A publication Critical patent/IN2012DN01992A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/20—Vector systems having a special element relevant for transcription transcription of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Chemical Kinetics & Catalysis (AREA)

Abstract

An expression vector, comprising a first reporter nucleic acid sequence operably linked to a first expression control sequence comprising a promoter; and a second reporter nucleic acid sequence operably linked to a second expression control sequence that comprises a response element that is activated or inactivated as one or more of the cells differentiate or dedifferentiate. Methods and kits for imaging and monitoring stem cells comprising the expression vector are also provided.
IN1992DEN2012 2009-09-09 2010-09-06 IN2012DN01992A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12/555,901 US9719146B2 (en) 2009-09-09 2009-09-09 Composition and method for imaging stem cells
PCT/EP2010/063054 WO2011029798A1 (en) 2009-09-09 2010-09-06 Composition and method for imaging stem cells

Publications (1)

Publication Number Publication Date
IN2012DN01992A true IN2012DN01992A (en) 2015-07-24

Family

ID=43034378

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1992DEN2012 IN2012DN01992A (en) 2009-09-09 2010-09-06

Country Status (10)

Country Link
US (2) US9719146B2 (en)
EP (1) EP2475780B1 (en)
JP (1) JP6106436B2 (en)
KR (1) KR101760533B1 (en)
CN (1) CN102803508B (en)
AU (1) AU2010294316B2 (en)
CA (1) CA2773624C (en)
IN (1) IN2012DN01992A (en)
SG (1) SG179561A1 (en)
WO (1) WO2011029798A1 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011118655A1 (en) * 2010-03-23 2011-09-29 オリンパス株式会社 Method for monitoring state of differentiation in stem cells
US20130137604A1 (en) * 2011-11-30 2013-05-30 Raytheon Bbn Technologies Corp. Methods of evaluating genetic elements
US9719127B2 (en) * 2012-03-20 2017-08-01 The Regents Of The University Of California Dual inducible vectors and cell lines
ES2725827T3 (en) * 2012-05-23 2019-09-27 Univ Kyoto Highly efficient method to establish an artificial pluripotential stem cell
JP6116226B2 (en) * 2012-06-11 2017-04-19 オリンパス株式会社 How to identify stem cell status
JP2014176364A (en) * 2013-03-15 2014-09-25 Olympus Corp Analysis method of stem cells
JP6223037B2 (en) * 2013-07-22 2017-11-01 オリンパス株式会社 Survivin cell analysis method, and cancer analysis method and drug efficacy screening method using the same
JP6454478B2 (en) 2013-09-12 2019-01-16 オリンパス株式会社 Methods for monitoring cardiomyocyte differentiation
US20180363070A9 (en) * 2014-08-12 2018-12-20 Univ Wayne State Systems and methods to detect stem cell stress and uses thereof
WO2018122932A1 (en) * 2016-12-26 2018-07-05 オリンパス株式会社 Cell sorting method, method for manufacturing purified cell population, and luminescence imaging system
US11124847B1 (en) * 2017-10-03 2021-09-21 Nzumbe, Inc. Systems and methods for assessing perturbations in cellular differentiation using reporter cell lines
CN114164233A (en) * 2021-11-05 2022-03-11 贵州医科大学附属医院 pProTG plasmid, construction method and application thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7008776B1 (en) * 1996-12-06 2006-03-07 Aventis Pharmaceuticals Inc. Compositions and methods for effecting the levels of high density lipoprotein (HDL) cholesterol and apolipoprotein AI very low density lipoprotein (VLDL) cholesterol and low density lipoprotein (LDL) cholesterol
DE19710643A1 (en) * 1997-03-14 1998-09-17 Hoechst Ag The promoter of the cdc25B gene and its use in gene therapy
US6482937B1 (en) 1997-10-09 2002-11-19 Biotransplant, Inc. Porcine Oct-4 promoter
EP1250349A4 (en) 2000-01-14 2005-10-19 Beth Israel Hospital Cardiac-cell specific enhancer elements and uses thereof
WO2003018780A1 (en) 2001-08-27 2003-03-06 Advanced Cell Technology, Inc. De-differentiation and re-differentiation of somatic cells and production of cells for cell therapies
EP1474434B1 (en) 2002-02-15 2009-01-07 The Johns Hopkins University The eaat2 promoter and uses thereof
US7399851B2 (en) 2002-07-25 2008-07-15 Dana Farber Cancer Institute, Inc. Composition and method for imaging cells
US7524674B2 (en) 2003-03-07 2009-04-28 Stanford University Multimodality imaging of reporter gene expression using a novel fusion vector in living cells and animals
AU2004233315A1 (en) 2003-04-24 2004-11-04 Fondazione Centro San Raffaele Del Monte Tabor Lentiviral vectors carrying synthetic bi-directional promoters and uses thereof
US20050101017A1 (en) * 2003-11-10 2005-05-12 Wojtek Auerbach Method of improving gene targeting using a ubiquitin promoter
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
EP1727806A1 (en) 2004-01-16 2006-12-06 Novartis AG 2,4-diaminopyrimidines and their use for inducing cardiomyogenesis
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US9556238B2 (en) 2006-06-08 2017-01-31 Multi-Magnetics Inc. Magnetosome gene expression in eukaryotic cells
WO2008089396A1 (en) 2007-01-19 2008-07-24 Invitrogen Corporation Compositions and methods for genetic manipulation and monitoring of cell lines
WO2008144580A2 (en) 2007-05-17 2008-11-27 Oregon Health & Science University Primate stem cells produced by somatic cell nuclear transfer
WO2008144052A2 (en) 2007-05-18 2008-11-27 Rampyari Walia Bioluminescent imaging of stem cells
US20090137023A1 (en) 2007-10-16 2009-05-28 Brandon John Casutt Cytoplasm of Eukaryotic cells for reprogramming of somatic cells, healing, repairing and therapeutic applications
KR100952368B1 (en) 2007-11-29 2010-04-09 고려대학교 산학협력단 Development of dual promoter-driven reporter systems and their application in simultaneous tracing of mesenchymal stem cells differentiating into the cardiomyogenic or the endothelial cell lineage

Also Published As

Publication number Publication date
WO2011029798A1 (en) 2011-03-17
KR20120089667A (en) 2012-08-13
JP6106436B2 (en) 2017-03-29
CN102803508B (en) 2015-10-21
AU2010294316B2 (en) 2015-07-23
EP2475780A1 (en) 2012-07-18
AU2010294316A1 (en) 2012-03-22
US10920287B2 (en) 2021-02-16
KR101760533B1 (en) 2017-07-21
US20170298455A1 (en) 2017-10-19
JP2013503648A (en) 2013-02-04
CN102803508A (en) 2012-11-28
EP2475780B1 (en) 2015-03-04
SG179561A1 (en) 2012-05-30
US20110059439A1 (en) 2011-03-10
CA2773624C (en) 2019-07-30
CA2773624A1 (en) 2011-03-17
US9719146B2 (en) 2017-08-01

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