IL311366B2 - Stem/progenitor cells from duodenal Brunner's glands and methods for isolating and using them - Google Patents

Stem/progenitor cells from duodenal Brunner's glands and methods for isolating and using them

Info

Publication number
IL311366B2
IL311366B2 IL311366A IL31136624A IL311366B2 IL 311366 B2 IL311366 B2 IL 311366B2 IL 311366 A IL311366 A IL 311366A IL 31136624 A IL31136624 A IL 31136624A IL 311366 B2 IL311366 B2 IL 311366B2
Authority
IL
Israel
Prior art keywords
months
bgsc
isolated
culture conditions
population
Prior art date
Application number
IL311366A
Other languages
English (en)
Hebrew (he)
Other versions
IL311366B1 (en
IL311366A (en
Original Assignee
Univ North Carolina Chapel Hill
Sapienza Univ Di Roma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina Chapel Hill, Sapienza Univ Di Roma filed Critical Univ North Carolina Chapel Hill
Publication of IL311366A publication Critical patent/IL311366A/en
Publication of IL311366B1 publication Critical patent/IL311366B1/en
Publication of IL311366B2 publication Critical patent/IL311366B2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/38—Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/407—Liver; Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/067—Hepatocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2521/00—Culture process characterised by the use of hydrostatic pressure, flow or shear forces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2527/00—Culture process characterised by the use of mechanical forces, e.g. strain, vibration
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Veterinary Medicine (AREA)
  • Physiology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
IL311366A 2018-03-29 2019-03-28 Stem/progenitor cells from duodenal Brunner's glands and methods for isolating and using them IL311366B2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862650208P 2018-03-29 2018-03-29
PCT/US2019/024543 WO2019191402A1 (en) 2018-03-29 2019-03-28 Stem/progenitor cells from duodenal brunner's glands and methods of isolating and using them

Publications (3)

Publication Number Publication Date
IL311366A IL311366A (en) 2024-05-01
IL311366B1 IL311366B1 (en) 2025-01-01
IL311366B2 true IL311366B2 (en) 2025-05-01

Family

ID=68054860

Family Applications (2)

Application Number Title Priority Date Filing Date
IL311366A IL311366B2 (en) 2018-03-29 2019-03-28 Stem/progenitor cells from duodenal Brunner's glands and methods for isolating and using them
IL277552A IL277552B2 (en) 2018-03-29 2019-03-28 Stem/progenitor cells from Brunner's duodenal glands and methods for their isolation and use

Family Applications After (1)

Application Number Title Priority Date Filing Date
IL277552A IL277552B2 (en) 2018-03-29 2019-03-28 Stem/progenitor cells from Brunner's duodenal glands and methods for their isolation and use

Country Status (15)

Country Link
US (1) US20190300849A1 (enExample)
EP (1) EP3781181A4 (enExample)
JP (2) JP7562416B2 (enExample)
KR (1) KR20200140836A (enExample)
CN (1) CN112272698A (enExample)
AR (1) AR115304A1 (enExample)
AU (1) AU2019242887A1 (enExample)
BR (1) BR112020019773A2 (enExample)
CA (1) CA3112650A1 (enExample)
IL (2) IL311366B2 (enExample)
MX (1) MX2020010223A (enExample)
PH (1) PH12020551584A1 (enExample)
SG (1) SG11202009428YA (enExample)
TW (1) TW202002997A (enExample)
WO (1) WO2019191402A1 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG10202104575WA (en) 2016-11-04 2021-06-29 Childrens Hospital Med Ct Liver organoid compositions and methods of making and using same
CA3106634A1 (en) 2018-07-26 2020-01-30 Children's Hospital Medical Center Hepato-biliary-pancreatic tissues and methods of making same
CN112823012A (zh) 2018-09-12 2021-05-18 儿童医院医学中心 用于产生造血干细胞及其衍生物的类器官组合物
WO2020160371A1 (en) 2019-02-01 2020-08-06 The University Of Hong Kong Innervated organoid compositions and methods of making same
CA3141807A1 (en) 2019-05-31 2020-12-03 Children's Hospital Medical Center Methods of generating and expanding hematopoietic stem cells
US12534709B2 (en) 2019-05-31 2026-01-27 Children's Hospital Medical Center Shaped organoid compositions and methods of making same
KR102322635B1 (ko) * 2021-06-11 2021-11-05 인제대학교 산학협력단 말초신경양 미세조직 제조방법 및 이의 용도
AU2022304681A1 (en) * 2021-07-02 2024-01-25 Children’S Hospital Medical Center Structurally complete organoids
WO2023171702A1 (ja) * 2022-03-08 2023-09-14 慶應義塾 オルガノイドの製造方法
KR20250118284A (ko) * 2024-01-29 2025-08-06 (의) 삼성의료재단 중간엽 줄기세포 유래 췌도 오가노이드의 제조방법

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9212382B2 (en) * 2009-05-01 2015-12-15 Alfagene Bioscience, Inc. Human gastrointestinal stem cell-derived primary intestinal epithelial cell system and methods of use thereof
US20160058798A1 (en) * 2013-03-13 2016-03-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree
US20160084824A1 (en) * 2008-04-23 2016-03-24 Alfagene Bioscience, Inc. Drug discovery methods involving a preclnical, in vitro isolated gastrointestinal epithelial stem cell-like progenitor cell system

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002057430A2 (en) * 2001-01-20 2002-07-25 Cardion Ag Pluripotent adult stem cells derived from regenerative tissue
US20050256077A1 (en) 2004-03-30 2005-11-17 Henning Susan J Gastrointestinal stem cells and uses thereof
US20070026520A1 (en) * 2005-07-29 2007-02-01 Kelly James H Novel cells, compositions, and methods
US20070128584A1 (en) 2005-12-01 2007-06-07 Illani Atwater Method for isolation of cells
CN101668848B (zh) * 2007-04-26 2014-10-22 雷蒙特亚特特拉维夫大学有限公司 来自口腔粘膜的多能自体干细胞和使用方法
EP2326336B1 (en) * 2008-07-30 2015-05-13 Mesynthes Limited Tissue scaffolds derived from forestomach extracellular matrix
US8895300B2 (en) * 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
ES2819028T3 (es) * 2009-10-30 2021-04-14 Univ North Carolina Chapel Hill Células madre multipotentes del árbol biliar extrahepático y métodos para su aislamiento
WO2012070014A2 (en) * 2010-11-26 2012-05-31 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Identification of novel cell surface markers for pancreatic progenitor cells and definite endodermal cells
CN105087479A (zh) * 2015-08-21 2015-11-25 深圳爱生再生医学科技有限公司 干细胞无血清培养基及干细胞的培养方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160084824A1 (en) * 2008-04-23 2016-03-24 Alfagene Bioscience, Inc. Drug discovery methods involving a preclnical, in vitro isolated gastrointestinal epithelial stem cell-like progenitor cell system
US9212382B2 (en) * 2009-05-01 2015-12-15 Alfagene Bioscience, Inc. Human gastrointestinal stem cell-derived primary intestinal epithelial cell system and methods of use thereof
US20160058798A1 (en) * 2013-03-13 2016-03-03 University Of North Carolina At Chapel Hill Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree

Also Published As

Publication number Publication date
BR112020019773A2 (pt) 2021-02-17
CA3112650A1 (en) 2019-10-03
IL277552B1 (en) 2024-04-01
IL311366B1 (en) 2025-01-01
SG11202009428YA (en) 2020-10-29
MX2020010223A (es) 2021-01-15
KR20200140836A (ko) 2020-12-16
IL277552B2 (en) 2024-08-01
JP7562416B2 (ja) 2024-10-07
EP3781181A1 (en) 2021-02-24
JP2021519585A (ja) 2021-08-12
JP2024075687A (ja) 2024-06-04
EP3781181A4 (en) 2021-12-29
IL277552A (en) 2020-11-30
US20190300849A1 (en) 2019-10-03
AU2019242887A1 (en) 2020-11-12
PH12020551584A1 (en) 2021-09-13
CN112272698A (zh) 2021-01-26
IL311366A (en) 2024-05-01
WO2019191402A1 (en) 2019-10-03
AR115304A1 (es) 2020-12-23
TW202002997A (zh) 2020-01-16

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