IL259837B2 - Microfluidic devices and kits and their uses - Google Patents

Microfluidic devices and kits and their uses

Info

Publication number
IL259837B2
IL259837B2 IL259837A IL25983718A IL259837B2 IL 259837 B2 IL259837 B2 IL 259837B2 IL 259837 A IL259837 A IL 259837A IL 25983718 A IL25983718 A IL 25983718A IL 259837 B2 IL259837 B2 IL 259837B2
Authority
IL
Israel
Prior art keywords
situ
assay
capture structure
microfluidic device
generated capture
Prior art date
Application number
IL259837A
Other languages
English (en)
Hebrew (he)
Other versions
IL259837A (en
IL259837B (en
Original Assignee
Berkeley Lights Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Berkeley Lights Inc filed Critical Berkeley Lights Inc
Publication of IL259837A publication Critical patent/IL259837A/en
Publication of IL259837B publication Critical patent/IL259837B/en
Publication of IL259837B2 publication Critical patent/IL259837B2/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • G01N33/54386Analytical elements
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545Synthetic resin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0647Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668Trapping microscopic beads
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/06Auxiliary integrated devices, integrated components
    • B01L2300/0681Filter
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0424Dielectrophoretic forces
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/04Moving fluids with specific forces or mechanical means
    • B01L2400/0403Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427Electrowetting

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Fluid Mechanics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
IL259837A 2015-12-08 2016-12-07 Microfluidic devices and kits and their uses IL259837B2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201562264665P 2015-12-08 2015-12-08
US201662333821P 2016-05-09 2016-05-09
US201662418625P 2016-11-07 2016-11-07
PCT/US2016/065426 WO2017100347A1 (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and methods for use thereof

Publications (3)

Publication Number Publication Date
IL259837A IL259837A (en) 2018-07-31
IL259837B IL259837B (en) 2022-11-01
IL259837B2 true IL259837B2 (en) 2023-03-01

Family

ID=57590894

Family Applications (2)

Application Number Title Priority Date Filing Date
IL297205A IL297205A (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and their uses
IL259837A IL259837B2 (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and their uses

Family Applications Before (1)

Application Number Title Priority Date Filing Date
IL297205A IL297205A (en) 2015-12-08 2016-12-07 Microfluidic devices and kits and their uses

Country Status (12)

Country Link
US (3) US10705082B2 (cg-RX-API-DMAC7.html)
EP (2) EP4102226A1 (cg-RX-API-DMAC7.html)
JP (2) JP7083752B2 (cg-RX-API-DMAC7.html)
KR (2) KR20220153685A (cg-RX-API-DMAC7.html)
CN (2) CN108603878A (cg-RX-API-DMAC7.html)
AU (1) AU2016365724B2 (cg-RX-API-DMAC7.html)
CA (1) CA3005077C (cg-RX-API-DMAC7.html)
DK (1) DK3387438T3 (cg-RX-API-DMAC7.html)
IL (2) IL297205A (cg-RX-API-DMAC7.html)
SG (1) SG11201804275TA (cg-RX-API-DMAC7.html)
TW (2) TWI758265B (cg-RX-API-DMAC7.html)
WO (1) WO2017100347A1 (cg-RX-API-DMAC7.html)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL299366A (en) 2015-11-23 2023-02-01 Berkeley Lights Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
CN115184315A (zh) 2016-04-15 2022-10-14 伯克利之光生命科技公司 用于坞内测定的方法、系统和试剂盒
EP3551745A4 (en) 2016-12-12 2020-07-15 Xcella Biosciences, Inc. SCREENING METHODS AND SYSTEMS USING MICROCAPILLARY NETWORKS
US11156626B2 (en) 2016-12-30 2021-10-26 xCella Biosciences, Inc. Multi-stage sample recovery system
WO2018165432A1 (en) * 2017-03-08 2018-09-13 The Regents Of The University Of Michigan Functionalization of polymer scaffolds
WO2018226900A2 (en) 2017-06-06 2018-12-13 Zymergen Inc. A htp genomic engineering platform for improving fungal strains
KR102480894B1 (ko) * 2017-08-01 2022-12-23 일루미나, 인코포레이티드 뉴클레오티드 서열분석용 하이드로겔 비드
DE102017121914A1 (de) * 2017-09-21 2019-03-21 Endress+Hauser Conducta Gmbh+Co. Kg Sensorelement und Verfahren zum Herstellen eines Sensorelements
EP3721209B1 (en) 2017-10-15 2024-02-07 Berkeley Lights, Inc. Methods for in-pen assays
TWI652658B (zh) 2017-12-12 2019-03-01 奇景光電股份有限公司 晶片以及使用其的電子裝置
WO2019133874A1 (en) * 2017-12-31 2019-07-04 Berkeley Lights, Inc. General functional assay
KR102437660B1 (ko) 2018-02-13 2022-08-29 일루미나, 인코포레이티드 하이드로겔 비드를 사용한 dna 서열분석
JP7511344B2 (ja) 2018-04-20 2024-07-05 イルミナ インコーポレイテッド 単一細胞を封入する方法、封入された細胞およびその使用
KR20210018219A (ko) 2018-06-06 2021-02-17 지머젠 인코포레이티드 발효 및 생산 중 곰팡이 형태를 제어하기 위한 신호 전달에 포함된 유전자의 조작
WO2019245525A1 (en) * 2018-06-18 2019-12-26 Hewlett-Packard Development Company, L.P. Microfluidic immunoassays
KR20210078510A (ko) * 2018-10-11 2021-06-28 버클리 라잇츠, 인크. 최적화된 단백질 생성의 확인을 위한 시스템 및 방법, 및 이를 위한 키트
CN111036315B (zh) * 2018-10-15 2021-09-21 京东方科技集团股份有限公司 一种微量样品的生成方法及生成芯片
CA3113271A1 (en) 2018-10-26 2020-04-30 Illumina, Inc. Modulating polymer beads for dna processing
KR20210089193A (ko) * 2018-11-01 2021-07-15 버클리 라잇츠, 인크. 미세유체 디바이스에서 생물학적 세포를 분석하기 위한 방법
US12318775B2 (en) 2018-12-06 2025-06-03 xCella Biosciences, Inc. Lateral loading of microcapillary arrays
JP7231923B2 (ja) * 2019-01-15 2023-03-02 国立大学法人東京工業大学 複合体の検出方法、並びにそれに用いる担体及び検出キット
US20220146502A1 (en) * 2019-02-01 2022-05-12 Sentilus Holdco, Llc High-sensitivity assay
CN110054146B (zh) * 2019-03-18 2021-08-24 北京航空航天大学 一种周期性v型微柱结构复合膜及其制备方法与应用
CN116174068A (zh) * 2019-04-30 2023-05-30 伯克利之光生命科技公司 用于包封和测定细胞的方法
US11668676B2 (en) * 2019-05-30 2023-06-06 The Regents Of The University Of California Microfluidic acoustic devices and methods
WO2021067401A1 (en) * 2019-09-30 2021-04-08 University Of Florida Research Foundation, Incorporated Use of ganglioside 2 and 3 for circulating sarcoma cell detection
CA3157616A1 (en) 2019-11-17 2021-05-20 Darshan THAKER Systems and methods for analyses of biological samples
CN111046607B (zh) * 2019-12-12 2025-05-13 浙江清华柔性电子技术研究院 柔性微流体器件设计方法及柔性微流体器件
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
WO2022109075A1 (en) * 2020-11-17 2022-05-27 Xbiologix, Inc. Configurable substrate of a fluidic device
US12377415B2 (en) 2020-12-30 2025-08-05 The Trustees Of The University Of Pennsylvania Photoactivated selective release (PHASR) of droplets from microwell arrays
TW202248635A (zh) * 2021-04-06 2022-12-16 美商伯克利之光生命科技公司 微流體分析與來自非哺乳動物細胞之生物生產的方法及用於其之套組
EP4444910A4 (en) * 2021-12-10 2025-11-05 Bio Rad Laboratories Inc Compositions, processes and systems for the treatment of samples with functionalized particles of adjustable morphology
EP4469207A4 (en) * 2022-01-25 2025-12-31 Bruker Cellular Analysis Inc METHODS FOR DOSING MICRO-OBJECTS IN A MICROFLUID DEVICE
EP4619551A2 (en) * 2022-11-17 2025-09-24 Bruker Cellular Analysis, Inc. Methods of evaluating virus-producing cells
WO2025068547A1 (en) * 2023-09-28 2025-04-03 Zhen Zhang A microfluidic device, a microfluidic system and a method for the capture and controllable release of single biological cells

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008131048A2 (en) * 2007-04-16 2008-10-30 Cellpoint Diagnotics, Inc. Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells
WO2015061506A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
WO2015061462A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Exporting a selected group of micro-objects from a micro-fluidic device
WO2015061497A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Family Cites Families (109)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997040385A1 (en) 1996-04-25 1997-10-30 Bioarray Solutions, Llc Light-controlled electrokinetic assembly of particles near surfaces
US6387707B1 (en) 1996-04-25 2002-05-14 Bioarray Solutions Array Cytometry
JP2002536640A (ja) 1999-02-03 2002-10-29 アクララ バイオサイエンシーズ, インコーポレイテッド 微小流体導入の際のマルチチャネル制御
US6294063B1 (en) 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
US6942776B2 (en) 1999-05-18 2005-09-13 Silicon Biosystems S.R.L. Method and apparatus for the manipulation of particles by means of dielectrophoresis
EP1194693B1 (en) 1999-06-28 2006-10-25 California Institute Of Technology Microfabricated elastomeric valve and pump systems
JP2003505260A (ja) 1999-07-23 2003-02-12 ザ ボード オブ トラスティーズ オブ ザ ユニバーシテイ オブ イリノイ 微小成形加工された装置及びその製造方法
CA2394942A1 (en) 1999-12-20 2001-06-28 Stephen J. Fonash Deposited thin films and their use in detection, attachment, and bio-medical applications
US20030007894A1 (en) 2001-04-27 2003-01-09 Genoptix Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles
EP2381116A1 (en) * 2000-11-16 2011-10-26 California Institute of Technology Apparatus and methods for conducting assays and high throughput screening
ITTO20010411A1 (it) 2001-05-02 2002-11-02 Silicon Biosystems S R L Metodo e dispositivo per l'esecuzione di test e saggi ad alta processivita' ed alto valore biologico su cellule e/o composti.
US20030175947A1 (en) 2001-11-05 2003-09-18 Liu Robin Hui Enhanced mixing in microfluidic devices
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
JP4228114B2 (ja) * 2002-05-07 2009-02-25 独立行政法人科学技術振興機構 微小物体の回収方法及び装置
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
DE10224352A1 (de) * 2002-06-01 2003-12-11 Mueller Schulte Detlef Thermosensitive Polymerträger mit veränderbarer physikalischer Struktur für die biochemische Analytik, Diagnostik und Therapie
US7790443B2 (en) 2002-08-27 2010-09-07 Vanderbilt University Bioreactors with substance injection capacity
CA2512071A1 (en) * 2002-12-30 2004-07-22 The Regents Of The University Of California Methods and apparatus for pathogen detection and analysis
CN101613660B (zh) * 2002-12-30 2013-05-15 加州大学评议会 检测和分析病原体的方法和设备
WO2004065618A2 (en) 2003-01-16 2004-08-05 Thermogenic Imaging Methods and devices for monitoring cellular metabolism in microfluidic cell-retaining chambers
AU2004228678A1 (en) 2003-04-03 2004-10-21 Fluidigm Corp. Microfluidic devices and methods of using same
US20060240548A1 (en) 2003-06-26 2006-10-26 Mordechai Deutsch Materials for constructing cell-chips, cell-chip covers, cell-chips coats, processed cell-chips and uses thereof
US20050164402A1 (en) 2003-07-14 2005-07-28 Belisle Christopher M. Sample presentation device
EP1664265A4 (en) 2003-07-21 2009-11-25 Seng Entpr Ltd IMPROVED MULTIPLAY PLATE
US20060194306A1 (en) * 2003-08-25 2006-08-31 Herr Amy E System for gel electrophoretic immunoassay
JP4328168B2 (ja) 2003-10-02 2009-09-09 ソニー株式会社 毛細管現象を利用する物質間の相互作用検出部と該検出部を用いる方法及びバイオアッセイ用基板
US20070105119A1 (en) 2003-10-29 2007-05-10 Zhinqiang Gao Method for detecting analytes by means of an analyte/polymeric activator bilayer arrangement
WO2005072399A2 (en) 2004-01-29 2005-08-11 Massachusetts Institute Of Technology Microscale sorting cytometer
US8765454B2 (en) 2004-02-18 2014-07-01 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
US20050221473A1 (en) 2004-03-30 2005-10-06 Intel Corporation Sensor array integrated circuits
JP2007537729A (ja) 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット
US7935479B2 (en) 2004-07-19 2011-05-03 Cell Biosciences, Inc. Methods and devices for analyte detection
US7258990B2 (en) * 2004-08-19 2007-08-21 Biocept, Inc. Alleviation of non-specific binding in microarray assays
US20080014575A1 (en) 2004-10-25 2008-01-17 University Of Washington Rapid Microfluidic Assay for Quantitative Measurement of Interactions Among One or More Analytes
US20060091015A1 (en) 2004-11-01 2006-05-04 Applera Corporation Surface modification for non-specific adsorption of biological material
US20060237080A1 (en) * 2005-02-15 2006-10-26 Sangyong Jon Patterned surfaces and polymeric microstructures within robust microfluidic channels
JP2006308332A (ja) 2005-04-26 2006-11-09 Moritex Corp 固定反応物質と液体反応物質との反応用フローセル及び反応装置並びに反応装置の運転方法
TWI265864B (en) 2005-05-20 2006-11-11 Univ Nat Cheng Kung Hydrophilic surface structure of non-hydrophilic substrate and fabricating method for the same
EP3029135B1 (en) 2005-07-07 2021-03-17 The Regents of the University of California Apparatus for cell culture array
CA2616504A1 (en) 2005-08-19 2007-03-01 The Regents Of The University Of California Microfluidic methods for diagnostics and cellular analysis
WO2007102839A2 (en) 2005-10-27 2007-09-13 Applera Corporation Optoelectronic separation of biomolecules
TW200734641A (en) * 2005-12-26 2007-09-16 Inst Of Microchemical Technology Microchip for immunoassay, kit for immunoassay and immunoassay method
US20090011486A1 (en) * 2006-01-12 2009-01-08 Massachusetts Institute Of Technology Biodegradable Elastomers
WO2007092713A2 (en) 2006-02-02 2007-08-16 Trustees Of The University Of Pennsylvania Microfluidic system and method for analysis of gene expression in cell-containing samples and detection of disease
US8124015B2 (en) 2006-02-03 2012-02-28 Institute For Systems Biology Multiplexed, microfluidic molecular assay device and assay method
CA2641550A1 (en) 2006-02-07 2007-08-16 Wafergen, Inc. Temperature-regulated culture plates
US20150107995A1 (en) 2006-04-18 2015-04-23 Advanced Liquid Logic, Inc. Droplet Actuator Devices and Methods for Manipulating Beads
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
WO2010048279A2 (en) * 2008-10-21 2010-04-29 Massachusetts Institute Of Technology Lock-release polymerization
US7790631B2 (en) 2006-11-21 2010-09-07 Intel Corporation Selective deposition of a dielectric on a self-assembled monolayer-adsorbed metal
US20080153134A1 (en) 2006-12-22 2008-06-26 Willy Wiyatno Methods and apparatus for generating hydrophilic patterning of high density microplates using an amphiphilic polymer
WO2008083323A1 (en) 2006-12-29 2008-07-10 Invitrogen Corporation Detection apparatus
EP2125219B1 (en) 2007-01-19 2016-08-10 Fluidigm Corporation High precision microfluidic devices and methods
US8685344B2 (en) 2007-01-22 2014-04-01 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
WO2008119066A1 (en) 2007-03-28 2008-10-02 The Regents Of The University Of California Single-sided lateral-field and phototransistor-based optoelectronic tweezers
ATE554859T1 (de) 2007-05-24 2012-05-15 Univ California Integrierte fluidische vorrichtungen mit magnetischer sortierung
US8961877B2 (en) 2007-08-09 2015-02-24 Massachusetts Institute Of Technology High-throughput, whole-animal screening system
WO2009061392A1 (en) 2007-11-05 2009-05-14 President And Fellows Of Harvard College Forming gel structures using microfluidic channels
CA2705213C (en) 2007-11-07 2016-10-04 The University Of British Columbia Microfluidic device and method of using same
EP2279238A2 (en) 2008-04-21 2011-02-02 Cell Kinetics, Ltd. Flat cell carriers with cell traps
CN101275114A (zh) 2008-04-22 2008-10-01 北京大学 一种微流细胞培养阵列及其应用
KR100991752B1 (ko) 2008-07-15 2010-11-03 한국과학기술원 단일 평면 광전자 소자를 이용한 미세입자 구동장치 및구동방법
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
JP2012522518A (ja) 2009-04-03 2012-09-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 細胞および生体粒子を選別するための方法および装置
US20100273681A1 (en) 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers
GB0909923D0 (en) 2009-06-09 2009-07-22 Oxford Gene Tech Ip Ltd Picowell capture devices for analysing single cells or other particles
JP2011000079A (ja) 2009-06-19 2011-01-06 Univ Of Tokyo 粒子を操作する方法及びマイクロ流体装置
WO2011044116A2 (en) 2009-10-05 2011-04-14 The Charles Stark Draper Laboratory, Inc. Three-dimensional microfluidic platforms and methods of use and manufacture thereof
US20130118905A1 (en) 2010-05-26 2013-05-16 Tosoh Corporation Biological sample immobilizing apparatus
CH703278B1 (fr) 2010-06-15 2016-11-15 Csem Centre Suisse D'electronique Et De Microtechnique Sa - Rech Et Développement Appareil et plateforme pour analyse multiplex.
CN101947124B (zh) 2010-06-25 2012-07-04 博奥生物有限公司 一种集成式微流控芯片装置及其使用方法
US20130115606A1 (en) 2010-07-07 2013-05-09 The University Of British Columbia System and method for microfluidic cell culture
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
US9533306B2 (en) 2010-08-02 2017-01-03 The Regents Of The University Of California Single sided continuous optoelectrowetting (SCEOW) device for droplet manipulation with light patterns
US20120040843A1 (en) * 2010-08-12 2012-02-16 Dublin City University Centrifugal capture system
CA2809877C (en) 2010-09-14 2021-11-23 The Regents Of The University Of California Method and device for isolating cells from heterogeneous solution using microfluidic trapping vortices
SG189160A1 (en) 2010-09-29 2013-05-31 Massachusetts Inst Technology Device for high throughput investigations of cellular interactions
WO2012040861A1 (en) 2010-10-01 2012-04-05 The Governing Council Of The University Of Toronto Digital microfluidic devices and methods incorporating a solid phase
AU2011312218B2 (en) 2010-10-04 2015-11-05 Sequencing Health, Inc. Systems and methods for automated reusable parallel biological reactions
US8581167B2 (en) 2010-11-16 2013-11-12 Palo Alto Research Center Incorporated Optically patterned virtual electrodes and interconnects on polymer and semiconductive substrates
CN103392124B (zh) 2010-12-03 2016-04-20 塞普莱有限公司 细胞功能的微分析
WO2012072823A1 (en) 2010-12-03 2012-06-07 Mindseeds Laboratories Srl Rapid screening of monoclonal antibodies
WO2012109068A2 (en) 2011-02-11 2012-08-16 Corning Incorporated Enzyme cleavable cell release polymeric surface
JP5093633B2 (ja) * 2011-02-28 2012-12-12 独立行政法人科学技術振興機構 複数物質応答性ゲルおよびその製造方法並びにその利用
CN103732731A (zh) 2011-05-27 2014-04-16 不列颠哥伦比亚大学 用于高通量分析的微流控细胞捕获和分析设备
US9227200B2 (en) 2011-06-03 2016-01-05 The Regents Of The University Of California Microfluidic devices with flexible optically transparent electrodes
EP2726870B1 (en) 2011-06-29 2018-10-03 Academia Sinica The capture, purification and release of biological substance using a surface coating
CA2842359A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
US9050593B2 (en) 2011-11-23 2015-06-09 Wisconsin Alumni Research Foundation Self-loading microfluidic device and methods of use
US9506845B2 (en) 2012-02-29 2016-11-29 Fluidigm Corporation Methods, systems and devices for multiple single-cell capturing and processing using microfluidics
US10626435B2 (en) 2012-03-28 2020-04-21 Northeastern University Nanofluidic device for isolating, growing, and characterizing microbial cells
KR101959447B1 (ko) 2012-04-06 2019-03-18 삼성전자주식회사 시료 중의 표적물질을 효율적으로 처리하는 방법
TWI512383B (zh) 2012-07-04 2015-12-11 Ind Tech Res Inst 光學感應式介電泳裝置
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9403172B2 (en) 2012-11-08 2016-08-02 Berkeley Lights, Inc. Circuit based optoelectronic tweezers
US9239328B2 (en) 2012-12-17 2016-01-19 Taiwan Semiconductor Manufacturing Company, Ltd. Systems and methods for an integrated bio-entity manipulation and processing semiconductor device
US9366647B2 (en) 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
KR102668221B1 (ko) 2013-03-28 2024-05-23 더 유니버시티 오브 브리티쉬 콜롬비아 미세유동 장치 및 분비물의 다세포 검정법에서의 그의 사용 방법
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
AU2015249294B2 (en) 2014-04-25 2020-02-27 Berkeley Lights, Inc. Providing DEP manipulation devices and controllable electrowetting devices in the same microfluidic apparatus
US20150306598A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. DEP Force Control And Electrowetting Control In Different Sections Of The Same Microfluidic Apparatus
US20150306599A1 (en) 2014-04-25 2015-10-29 Berkeley Lights, Inc. Providing DEP Manipulation Devices And Controllable Electrowetting Devices In The Same Microfluidic Apparatus
KR102369770B1 (ko) 2014-12-08 2022-03-02 버클리 라잇츠, 인크. 측방향/수직 트랜지스터 구조들을 포함하는 미세유체 디바이스 및 그 제조 및 사용 프로세스
WO2016094715A2 (en) 2014-12-10 2016-06-16 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
CA3176018A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Microfluidic device for culturing biological cells and methods of use thereof
IL299835A (en) 2015-12-30 2023-03-01 Berkeley Lights Inc Microfluidic devices for optically activated convection and displacement, and their kits and methods
CN115184315A (zh) 2016-04-15 2022-10-14 伯克利之光生命科技公司 用于坞内测定的方法、系统和试剂盒
WO2017205830A1 (en) 2016-05-26 2017-11-30 Berkeley Lights, Inc. Covalently modified surfaces, kits, and methods of preparation and use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008131048A2 (en) * 2007-04-16 2008-10-30 Cellpoint Diagnotics, Inc. Devices and methods for diagnosing, prognosing, or theranosing a condition by enriching rare cells
WO2015061506A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
WO2015061462A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Exporting a selected group of micro-objects from a micro-fluidic device
WO2015061497A1 (en) * 2013-10-22 2015-04-30 Berkeley Lights, Inc. Microfluidic devices having isolation pens and methods of testing biological micro-objects with same

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SAJEESH, P., AND ASHIS KUMAR SEN., PARTICLE SEPARATION AND SORTING IN MICROFLUIDIC DEVICES: A REVIEW., 31 December 2014 (2014-12-31) *

Also Published As

Publication number Publication date
CN114878807A (zh) 2022-08-09
DK3387438T3 (da) 2023-05-15
US10705082B2 (en) 2020-07-07
TW202221322A (zh) 2022-06-01
US20210011015A1 (en) 2021-01-14
TWI847100B (zh) 2024-07-01
US11454629B2 (en) 2022-09-27
AU2016365724A1 (en) 2018-06-21
IL297205A (en) 2022-12-01
IL259837A (en) 2018-07-31
JP7083752B2 (ja) 2022-06-13
EP3387438B1 (en) 2023-03-01
CN108603878A (zh) 2018-09-28
WO2017100347A1 (en) 2017-06-15
AU2016365724B2 (en) 2023-10-12
CA3005077A1 (en) 2017-06-15
EP3387438A1 (en) 2018-10-17
US20170184583A1 (en) 2017-06-29
TW201727234A (zh) 2017-08-01
US20230023831A1 (en) 2023-01-26
JP2022112033A (ja) 2022-08-01
TWI758265B (zh) 2022-03-21
EP4102226A1 (en) 2022-12-14
KR102466814B1 (ko) 2022-11-11
CA3005077C (en) 2023-02-28
IL259837B (en) 2022-11-01
SG11201804275TA (en) 2018-06-28
KR20220153685A (ko) 2022-11-18
KR20180091062A (ko) 2018-08-14
JP2018538531A (ja) 2018-12-27

Similar Documents

Publication Publication Date Title
US20230023831A1 (en) In-situ generated microfluidic assay structures, related kits, and methods of use thereof
US12138626B2 (en) In situ-generated microfluidic isolation structures, kits and methods of use thereof
US11802264B2 (en) Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
HK40077731A (en) Microfluidic devices and kits and methods for use thereof
HK1261471A1 (en) Microfluidic devices and kits and methods for use thereof