IE62803B1 - An isoelectric focusing process and a means for carrying out said process - Google Patents

An isoelectric focusing process and a means for carrying out said process

Info

Publication number
IE62803B1
IE62803B1 IE106788A IE106788A IE62803B1 IE 62803 B1 IE62803 B1 IE 62803B1 IE 106788 A IE106788 A IE 106788A IE 106788 A IE106788 A IE 106788A IE 62803 B1 IE62803 B1 IE 62803B1
Authority
IE
Ireland
Prior art keywords
isoelectric
amphoteric
gradients
flow
membranes
Prior art date
Application number
IE106788A
Other languages
English (en)
Other versions
IE881067L (en
Inventor
Daniel Michel Faupel
Pier Giorgio Righetti
Original Assignee
Ciba Geigy Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag
Publication of IE881067L publication Critical patent/IE881067L/xx
Publication of IE62803B1 publication Critical patent/IE62803B1/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44756Apparatus specially adapted therefor
    • G01N27/44795Isoelectric focusing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
IE106788A 1987-04-11 1988-04-08 An isoelectric focusing process and a means for carrying out said process IE62803B1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process

Publications (2)

Publication Number Publication Date
IE881067L IE881067L (en) 1988-10-11
IE62803B1 true IE62803B1 (en) 1995-03-08

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
IE106788A IE62803B1 (en) 1987-04-11 1988-04-08 An isoelectric focusing process and a means for carrying out said process

Country Status (12)

Country Link
US (2) US4971670A (OSRAM)
EP (1) EP0287513B1 (OSRAM)
JP (1) JPH0781987B2 (OSRAM)
CA (1) CA1335805C (OSRAM)
DE (1) DE3876273T2 (OSRAM)
DK (1) DK174962B1 (OSRAM)
ES (1) ES2037273T3 (OSRAM)
FI (1) FI97893C (OSRAM)
GR (1) GR3007124T3 (OSRAM)
HK (1) HK192495A (OSRAM)
IE (1) IE62803B1 (OSRAM)
PT (1) PT87210B (OSRAM)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5173160A (en) * 1991-02-27 1992-12-22 Board Of Regents, The University Of Texas System Analysis of carrier ampholytes using immobilized ph gradients
US5323846A (en) * 1992-01-14 1994-06-28 Fotodyne Incorporated Fluid circulator and temperature regulator
GB2267502B (en) * 1992-05-28 1997-01-22 Aligena Ag Polymeric reaction products for use in immobilized buffered gels and membranes
US6319472B1 (en) 1993-11-01 2001-11-20 Nanogen, Inc. System including functionally separated regions in electrophoretic system
US6225059B1 (en) 1993-11-01 2001-05-01 Nanogen, Inc. Advanced active electronic devices including collection electrodes for molecular biological analysis and diagnostics
US6375899B1 (en) 1993-11-01 2002-04-23 Nanogen, Inc. Electrophoretic buss for transport of charged materials in a multi-chamber system
US6331274B1 (en) 1993-11-01 2001-12-18 Nanogen, Inc. Advanced active circuits and devices for molecular biological analysis and diagnostics
US6129828A (en) * 1996-09-06 2000-10-10 Nanogen, Inc. Apparatus and methods for active biological sample preparation
US20040077074A1 (en) * 1993-11-01 2004-04-22 Nanogen, Inc. Multi-chambered analysis device
US5437774A (en) * 1993-12-30 1995-08-01 Zymogenetics, Inc. High molecular weight electrodialysis
US5480526A (en) * 1994-06-07 1996-01-02 Bio-Rad Laboratories, Inc. Methods for the desalting of biological samples: a simple approach to eliminate disturbances in isoelectric focusing caused by the presence of salts
US6071394A (en) 1996-09-06 2000-06-06 Nanogen, Inc. Channel-less separation of bioparticles on a bioelectronic chip by dielectrophoresis
US6403367B1 (en) * 1994-07-07 2002-06-11 Nanogen, Inc. Integrated portable biological detection system
US7857957B2 (en) * 1994-07-07 2010-12-28 Gamida For Life B.V. Integrated portable biological detection system
CN1051245C (zh) * 1995-01-20 2000-04-12 清华大学 制备型等电点电泳分离方法及设备
IT1272932B (it) * 1995-01-24 1997-07-01 Pier Giorgio Righetti Reattore ad enzima immobilizzato
US5540826A (en) * 1995-03-15 1996-07-30 Protein Technologies, Inc. Multi-channel separation device
US7824532B2 (en) 1995-04-26 2010-11-02 Life Technologies Corporation Apparatus and method for electrophoresis
ES2224173T3 (es) * 1995-07-05 2005-03-01 Aventis Bulk S.P.A. Purificacion de antibioticos dalbaheptidos por enfoque isoelectrico.
RU2140073C1 (ru) * 1998-01-30 1999-10-20 Стоянов Александр Владимирович Способ формирования сред с заданным профилем ph
DE19831210A1 (de) * 1998-07-03 2000-01-05 Wita Gmbh Wittmann Inst Of Tec Verfahren und Vorrichtung zur zwei-dimensionalen Trennung von Biomolekülen
AUPP521298A0 (en) * 1998-08-12 1998-09-03 Life Therapeutics Limited Purification of fibrinogen
SE9803224D0 (sv) * 1998-09-23 1998-09-23 Amersham Pharm Biotech Ab Method for separation of macromolecules
AUPP790698A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Separation of microorganisms
US6267579B1 (en) * 1998-12-23 2001-07-31 Clinical Laboratory Development Group, Inc. Apparatus for making a gradient gel
AUPP790898A0 (en) 1998-12-23 1999-01-28 Life Therapeutics Limited Renal dialysis
US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
AUPP971399A0 (en) 1999-04-12 1999-05-06 Life Therapeutics Limited Separation of plasma components
US6284115B1 (en) 1999-09-21 2001-09-04 Agilent Technologies, Inc. In-line flow through micro-dialysis apparatus and method for high performance liquid phase separations
US6758953B2 (en) 1999-10-28 2004-07-06 Nathan A. Thomas Multistage electrophoresis apparatus and method of use for the separation and purification of cells, particles and solutes
JP2003514829A (ja) * 1999-11-15 2003-04-22 プロテオム システムズ リミテッド 多室電気泳動
WO2001036071A1 (en) * 1999-11-16 2001-05-25 Champagne James T Solution based two-dimensional separation and detection of amphoteric substances
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
AU3812000A (en) * 2000-03-15 2001-09-24 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) * 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
ATE449200T1 (de) 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
GB0010957D0 (en) * 2000-05-05 2000-06-28 Novartis Ag Compound & method
US6537434B1 (en) 2000-07-21 2003-03-25 Large Scale Proteomics Corporation First dimension electrophoresis separation method and apparatus
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
EP1341596B1 (en) * 2000-10-06 2010-07-07 Arturus Capital Limited Multi-port electrophoresis separation apparatus and corresponding method
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
WO2002071024A2 (en) * 2001-03-08 2002-09-12 Ethrog Biotechnology Ltd. Apparatus and method for electrophoresis
US7166202B2 (en) * 2001-07-16 2007-01-23 Protein Forest, Inc. Matrixes, arrays, systems and methods
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
WO2004024752A1 (ja) 2002-09-11 2004-03-25 Chugai Seiyaku Kabushiki Kaisha タンパク質精製方法
US20060134620A1 (en) * 2002-11-28 2006-06-22 Mitsuharu Hirai Method and apparatus for concentration and purification of nucleic acid
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
US7459021B2 (en) * 2003-09-03 2008-12-02 Shmuel Bukshpan Methods and apparatus for rapid crystallization of biomolecules
EP1671112A4 (en) * 2003-10-07 2011-03-23 Life Technologies Corp IMPROVED GELE FOR ISOELECTRIC FOCUSING AND METHOD FOR USE THEREOF
WO2005045023A1 (ja) * 2003-11-10 2005-05-19 Arkray Inc. 核酸の濃縮精製方法および装置
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
JP2007529309A (ja) * 2004-03-17 2007-10-25 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド 混合物からタンパク質を分離、特定するための材料、方法、およびシステム
WO2005089910A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Multi-compartment filter and method of filtering using same
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
EP1888213B1 (en) * 2005-05-25 2017-10-18 Bio-Rad Laboratories, Inc. Fluidics device
WO2007018589A2 (en) * 2005-07-28 2007-02-15 Bio-Rad Laboratories, Inc. Separation of proteins based on isoelectric point using solid-phase buffers
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
EP2013613A4 (en) * 2006-04-27 2010-09-01 Ge Healthcare Bio Sciences Ab ELECTRODE BRIDGE
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
WO2009002459A2 (en) * 2007-06-22 2008-12-31 Massachusetts Institute Of Technology Free flow isoelectric focusing devices and methods
CA2699954A1 (en) * 2007-10-09 2009-04-16 Dalhousie University Apparatus for purifying molecules
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
CN104023551A (zh) 2011-11-04 2014-09-03 生物辐射实验室股份有限公司 细胞组分的同时纯化
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2773743B1 (en) * 2011-11-04 2019-05-22 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of ph
US9182372B2 (en) 2011-11-22 2015-11-10 Stephen G. Haralampu Stopped-flow, micro-fluidic device and method for the charge-based separation of complex analyte mixtures
WO2013123425A1 (en) 2012-02-15 2013-08-22 Bio-Rad Laboratories, Inc. Electronic control of ph and ionic strength
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
US10611826B2 (en) 2013-07-05 2020-04-07 Laboratoire Français Du Fractionnement Et Des Biotechnologies Affinity chromatography matrix
JP6462681B2 (ja) 2013-07-12 2019-01-30 ジェネンテック, インコーポレイテッド イオン交換クロマトグラフィーのインプットの最適化の解明

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3539493A (en) * 1967-08-31 1970-11-10 Canal Ind Corp Apparatus for preparative electrophoresis on gel support media
US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
GB2036081B (en) * 1977-06-15 1982-08-25 Nat Res Dev Membrane electrophoresis
EP0103965A2 (en) * 1982-08-20 1984-03-28 Imperial Chemical Industries Plc Electrofocusing apparatus
US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation

Also Published As

Publication number Publication date
DK174962B1 (da) 2004-03-29
EP0287513A2 (en) 1988-10-19
FI97893C (fi) 1997-03-10
DK191388A (da) 1988-10-12
EP0287513B1 (en) 1992-12-02
US5082548A (en) 1992-01-21
PT87210A (pt) 1989-05-12
FI881652A7 (fi) 1988-10-12
DK191388D0 (da) 1988-04-08
GR3007124T3 (OSRAM) 1993-07-30
ES2037273T3 (es) 1993-06-16
HK192495A (en) 1995-12-29
FI881652A0 (fi) 1988-04-08
JPH0781987B2 (ja) 1995-09-06
CA1335805C (en) 1995-06-06
PT87210B (pt) 1993-11-30
JPS63263457A (ja) 1988-10-31
FI97893B (fi) 1996-11-29
EP0287513A3 (en) 1990-10-10
DE3876273D1 (de) 1993-01-14
US4971670A (en) 1990-11-20
DE3876273T2 (de) 1993-05-27
IE881067L (en) 1988-10-11

Similar Documents

Publication Publication Date Title
EP0287513B1 (en) An isoelectric focusing process and a means for carrying out said process
Vesterberg [33] Isoelectric focusing of proteins
US4088561A (en) Apparatus for electrophoresis separation
Schwer et al. Analytical and micropreparative separation of peptides by capillary zone electrophoresis using discontinuous buffer systems
Righetti et al. Preparative protein purification in a multi-compartment electrolyser with immobiline membranes
Tiselius et al. Zone electrophoresis
Shuster [34] Preparative acrylamide gel electrophoresis: Continuous and disc techniques
Faupel et al. Isoelectric protein purification by orthogonally coupled hydraulic and electric transports in a segmented immobilized pH gradient
JP2003532894A (ja) 化合物の電気泳動分離
JP3410099B2 (ja) 担体両性電解質を使用しない等電点電気泳動方法及び装置
US20060151325A1 (en) Method for ph-biased isoelectric trapping separations
Catsimpoolas Isoelectric focusing and isotachophoresis of proteins
Galier et al. Study of the mass transfer phenomena involved in an electrophoretic membrane contactor
Righetti et al. A horizontal apparatus for isoelectric protein purification in a segmented immobilized pH gradient
Righetti et al. Enantiomer resolution in immobilized pH gradient gels via inclusion of a chiral discriminator
McCormick et al. Selective elution of zones from preparative isoelectric focusing gels by ampholytes or buffers
WO2010017109A1 (en) Systems and methods for quantitative analyte transfer
RILBE Trends in Instrumental and Methodical Development of Isoelectric Focusing
US20090314639A1 (en) Means and devices for electro-filtration of molecules
Lopez 2-D electrophoresis using carrier ampholytes in the first dimension (IEF)
Goto et al. Protein Separation by Preparative Multicompartment Electrolyzer Secluded by Isoelectric Membranes
CN101328206A (zh) 一种非变性的制备性电泳
Låås 12 Electrophoresis in Gels
Leaback et al. Isoelectric focusing of proteins
Hampson et al. Displacement electrohoresis in gel as a technique for separating proteins on a preparative scale

Legal Events

Date Code Title Description
MK9A Patent expired