DE3876273D1 - Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens. - Google Patents

Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens.

Info

Publication number
DE3876273D1
DE3876273D1 DE8888810226T DE3876273T DE3876273D1 DE 3876273 D1 DE3876273 D1 DE 3876273D1 DE 8888810226 T DE8888810226 T DE 8888810226T DE 3876273 T DE3876273 T DE 3876273T DE 3876273 D1 DE3876273 D1 DE 3876273D1
Authority
DE
Germany
Prior art keywords
carrying
isoelectric focusing
focusing method
isoelectric
focusing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8888810226T
Other languages
English (en)
Other versions
DE3876273T2 (de
Inventor
Pier Giorgio Prof Righetti
Daniel Michel Faupel
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB878708746A external-priority patent/GB8708746D0/en
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Application granted granted Critical
Publication of DE3876273D1 publication Critical patent/DE3876273D1/de
Publication of DE3876273T2 publication Critical patent/DE3876273T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
DE8888810226T 1987-04-11 1988-04-07 Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens. Expired - Lifetime DE3876273T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB878708746A GB8708746D0 (en) 1987-04-11 1987-04-11 Electrophoretic process
GB878728289A GB8728289D0 (en) 1987-04-11 1987-12-03 Isoelectric focusing process & means for carrying out said process

Publications (2)

Publication Number Publication Date
DE3876273D1 true DE3876273D1 (de) 1993-01-14
DE3876273T2 DE3876273T2 (de) 1993-05-27

Family

ID=26292133

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8888810226T Expired - Lifetime DE3876273T2 (de) 1987-04-11 1988-04-07 Isoelektrisches fokussierverfahren sowie einrichtung zur durchfuehrung dieses verfahrens.

Country Status (12)

Country Link
US (2) US4971670A (de)
EP (1) EP0287513B1 (de)
JP (1) JPH0781987B2 (de)
CA (1) CA1335805C (de)
DE (1) DE3876273T2 (de)
DK (1) DK174962B1 (de)
ES (1) ES2037273T3 (de)
FI (1) FI97893C (de)
GR (1) GR3007124T3 (de)
HK (1) HK192495A (de)
IE (1) IE62803B1 (de)
PT (1) PT87210B (de)

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US20050224355A1 (en) * 1999-12-23 2005-10-13 Brendon Conlan Removal of biological contaminants
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EP1235634A4 (de) * 1999-11-16 2004-09-01 Proteotools Llc Zweidimensionale trennung und detektion von in lösung befindlichen amphoteren substanzen
US7077942B1 (en) * 1999-12-23 2006-07-18 Gradipore Limited Removal of biological contaminants
US20080096284A1 (en) * 2000-02-08 2008-04-24 Regents Of The University Of Michigan Protein separation and analysis
AU3812000A (en) * 2000-03-15 2001-09-24 Proteosys Ag Micropreparative isoelectric focussing
US6638408B1 (en) * 2000-04-03 2003-10-28 The Wistar Institute Method and device for separation of charged molecules by solution isoelectric focusing
AUPQ691400A0 (en) 2000-04-14 2000-05-11 Life Therapeutics Limited Separation of micromolecules
AUPQ697300A0 (en) * 2000-04-18 2000-05-11 Life Therapeutics Limited Separation apparatus
ATE449200T1 (de) 2000-04-18 2009-12-15 Gradipore Ltd Trennung und behandlung von proben durch elektrophorese
GB0010957D0 (en) 2000-05-05 2000-06-28 Novartis Ag Compound & method
US6537434B1 (en) 2000-07-21 2003-03-25 Large Scale Proteomics Corporation First dimension electrophoresis separation method and apparatus
US6562213B1 (en) * 2000-08-30 2003-05-13 Ethrog Biotechnology Ltd. Electrophoresis apparatus for simultaneous loading of multiple samples
US6923896B2 (en) * 2000-09-22 2005-08-02 The Texas A&M University System Electrophoresis apparatus and method
AUPR051500A0 (en) 2000-09-29 2000-10-26 Proteome Systems Ltd Electrophoresis system
CN1235667C (zh) * 2000-10-06 2006-01-11 格拉迪普有限公司 多口分离装置及方法
AUPR222300A0 (en) * 2000-12-21 2001-01-25 Life Therapeutics Limited Electrophoresis device and method
EP1377527A4 (de) * 2001-03-08 2004-09-15 Ethrog Biotechnology Ltd Vorrichtung und verfahren zur elektrophorese
IL159810A0 (en) * 2001-07-16 2004-06-20 Protein Forest Inc Matrixes, arrays, systems and methods
GB0121189D0 (en) * 2001-08-31 2001-10-24 Diagnoswiss Sa Apparatus and method for separating an analyte
US6887362B2 (en) * 2002-02-06 2005-05-03 Nanogen, Inc. Dielectrophoretic separation and immunoassay methods on active electronic matrix devices
US8420789B2 (en) 2002-09-11 2013-04-16 Chugai Seiyaku Kabushiki Kaisha Method for removing DNA contaminants from a protein-containing sample
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WO2004104548A2 (en) * 2003-05-09 2004-12-02 Invitrogen Corporation Solution phase electrophoresis device, components, and methods
US7622028B2 (en) * 2003-05-09 2009-11-24 Life Technologies Corporation Solution phase electrophoresis device, components, and methods
WO2005021841A2 (en) * 2003-09-03 2005-03-10 Bioforms Methods and apparatus for rapid crystallization of biomolecules
WO2005036153A1 (en) * 2003-10-07 2005-04-21 Invitrogen Corporation Improved isoelectric focusing gels and methods of use thereof
CN1878863A (zh) * 2003-11-10 2006-12-13 爱科来株式会社 核酸的浓缩精制方法及装置
US20050197496A1 (en) * 2004-03-04 2005-09-08 Gtc Biotherapeutics, Inc. Methods of protein fractionation using high performance tangential flow filtration
WO2005089911A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Materials, methods, and systems for separating and identifying proteins from mixtures
WO2005089910A1 (en) * 2004-03-17 2005-09-29 Ciphergen Biosystems, Inc. Multi-compartment filter and method of filtering using same
US20050249667A1 (en) * 2004-03-24 2005-11-10 Tuszynski Jack A Process for treating a biological organism
US7407816B2 (en) * 2004-05-07 2008-08-05 Gentius, Inc Isoelectric particles and uses thereof
DE212005000044U1 (de) * 2004-08-25 2007-04-05 Agilent Technologies Inc., Santa Clara Elektrophoretische Separation in einem bewegten Fluid
US20060130159A1 (en) * 2004-12-09 2006-06-15 Nick Masiello Method of purifying recombinant MSP 1-42 derived from Plasmodium falciparum
EP1888213B1 (de) * 2005-05-25 2017-10-18 Bio-Rad Laboratories, Inc. Fluidvorrichtung
JP4976391B2 (ja) * 2005-07-28 2012-07-18 バイオ−ラド ラボラトリーズ インコーポレイテッド 固体緩衝剤を使用した等電点に基づくタンパク質の分離
US7531632B2 (en) * 2006-02-16 2009-05-12 Gtc Biotherapeutics, Inc. Clarification of transgenic milk using depth filtration
AU2007243994B2 (en) 2006-04-27 2012-08-02 Ge Healthcare Bio-Sciences Ab Electrodic bridge
US20080220442A1 (en) * 2006-12-06 2008-09-11 Proteinics Difference detection methods using isoelectric focusing chips
WO2009002459A2 (en) * 2007-06-22 2008-12-31 Massachusetts Institute Of Technology Free flow isoelectric focusing devices and methods
EP2214809A4 (de) * 2007-10-09 2011-12-28 Univ Dalhousie Vorrichtung zur reinigung von molekülen
US8282803B2 (en) 2009-03-25 2012-10-09 Bio-Rad Laboratories, Inc. Isoelectric focusing tray and electrode assembly for alternate gel strip orientations
US9766207B2 (en) 2011-11-04 2017-09-19 Bio-Rad Laboratories, Inc. Affinity methods and compositions employing electronic control of pH
EP2773221B1 (de) 2011-11-04 2019-01-09 Bio-Rad Laboratories, Inc. Gleichzeitige reinigung von zellkomponenten
US20130164860A1 (en) * 2011-11-04 2013-06-27 Technion Research & Development Foundation Ltd. Affinity methods and compositions employing electronic control of ph
CA2856779C (en) 2011-11-22 2021-07-20 Stephen G. Haralampu Stopped-flow, m icro-flu i imc device and method for the charge-based separation of complex analyte mixtures
EP2814597A4 (de) 2012-02-15 2015-10-28 Bio Rad Laboratories Elektronische steuerung von ph-wert und ionenstärke
US9321012B2 (en) 2012-04-04 2016-04-26 Bio-Rad Laboratories, Inc. Electronic protein fractionation
US9671368B2 (en) * 2013-05-10 2017-06-06 The Regents Of The University Of California Two-dimensional microfluidic devices and methods of using the same
TW201509432A (zh) 2013-07-05 2015-03-16 Lab Francais Du Fractionnement 親和層析基質
EP3536699A1 (de) 2013-07-12 2019-09-11 F. Hoffmann-La Roche AG Darstellung einer eingabeoptimierung in der ionenaustauschchromatografie

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US3844925A (en) * 1973-07-02 1974-10-29 Center For Blood Res Molecular fractionation
SE415731B (sv) * 1977-04-26 1980-10-27 Pharmacia Fine Chemicals Ab Vattenloslig amfolyt for separationsendamal samt sett att framstella densamma
DE2861803D1 (en) * 1977-06-15 1982-07-01 Nat Res Dev Electrophoresis membranes, their use in a separation method and separation apparatus
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US5114555A (en) * 1988-01-05 1992-05-19 Monsanto Company Continuous isoelectric separation

Also Published As

Publication number Publication date
US5082548A (en) 1992-01-21
IE62803B1 (en) 1995-03-08
DK191388D0 (da) 1988-04-08
JPH0781987B2 (ja) 1995-09-06
FI881652A (fi) 1988-10-12
EP0287513A3 (en) 1990-10-10
JPS63263457A (ja) 1988-10-31
DE3876273T2 (de) 1993-05-27
US4971670A (en) 1990-11-20
GR3007124T3 (de) 1993-07-30
ES2037273T3 (es) 1993-06-16
EP0287513A2 (de) 1988-10-19
CA1335805C (en) 1995-06-06
PT87210A (pt) 1989-05-12
DK191388A (da) 1988-10-12
FI97893B (fi) 1996-11-29
IE881067L (en) 1988-10-11
EP0287513B1 (de) 1992-12-02
DK174962B1 (da) 2004-03-29
PT87210B (pt) 1993-11-30
HK192495A (en) 1995-12-29
FI97893C (fi) 1997-03-10
FI881652A0 (fi) 1988-04-08

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NOVARTIS AG, BASEL, CH

8328 Change in the person/name/address of the agent

Representative=s name: PFENNING MEINIG & PARTNER GBR, 80339 MUENCHEN