HUP0004409A2 - Eljárás az Escherichia coli DSM 6601 törzs azonosítására - Google Patents

Eljárás az Escherichia coli DSM 6601 törzs azonosítására

Info

Publication number
HUP0004409A2
HUP0004409A2 HU0004409A HUP0004409A HUP0004409A2 HU P0004409 A2 HUP0004409 A2 HU P0004409A2 HU 0004409 A HU0004409 A HU 0004409A HU P0004409 A HUP0004409 A HU P0004409A HU P0004409 A2 HUP0004409 A2 HU P0004409A2
Authority
HU
Hungary
Prior art keywords
escherichia coli
strain dsm
sec
coli strain
muta
Prior art date
Application number
HU0004409A
Other languages
English (en)
Inventor
Gabriele Blum-Oehler
Jörg Hacker
Jürgen Malinka
Hans Proppert
Jürgen Schulze
Ulrich Sonnenborn
Original Assignee
Pharma-Zentrale Gmbh.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pharma-Zentrale Gmbh. filed Critical Pharma-Zentrale Gmbh.
Publication of HUP0004409A2 publication Critical patent/HUP0004409A2/hu
Publication of HUP0004409A3 publication Critical patent/HUP0004409A3/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A találmány tárgya: eljárás az Escherichia coli (E. coli) DSM 6601törzs azonosítására. Az eljárás során a Muta 1-Muta 10 DNS-szekvenciájú primer párokat PCR-reakciónak vetik alá a következőkörülmények között: a. 3 perc 95 °C-on, b. 45 sec 95 °C-on, c. 45 sec58 °C-on, d. 45 sec 72 °C-on, és a b-d. lépést legalább húszszormegismétlik. A találmány szerinti eljárást a gyógyászatbandiagnosztikus és terápiás célokra, valamint a géntechnológia területénalkalmazzák. Ó
HU0004409A 1997-11-19 1998-11-18 Method for identifying escherichia coli strain dsm 6601 HUP0004409A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19751243 1997-11-19
PCT/EP1998/007398 WO1999025870A1 (de) 1997-11-19 1998-11-18 Verfahren zur identifizierung von escherichia coli stamm dsm 6601

Publications (2)

Publication Number Publication Date
HUP0004409A2 true HUP0004409A2 (hu) 2001-04-28
HUP0004409A3 HUP0004409A3 (en) 2001-09-28

Family

ID=7849197

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0004409A HUP0004409A3 (en) 1997-11-19 1998-11-18 Method for identifying escherichia coli strain dsm 6601

Country Status (9)

Country Link
US (1) US6489107B1 (hu)
EP (1) EP1038035A1 (hu)
JP (1) JP2004516801A (hu)
CZ (1) CZ289739B6 (hu)
EE (1) EE200000231A (hu)
HU (1) HUP0004409A3 (hu)
NO (1) NO20002550L (hu)
PL (1) PL190847B1 (hu)
WO (1) WO1999025870A1 (hu)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19915772C2 (de) * 1998-11-18 2001-08-30 Pharma Zentrale Gmbh Verfahren zur Identifizierung von Escherichia coli Stamm DSM 6601
US20050064447A1 (en) * 2001-04-18 2005-03-24 Sheng-He Huang Probiotic therapy of neonatal meningitis and method of using E. coli virulence determinatns
DE10155928A1 (de) * 2001-11-15 2003-06-12 Degussa recA-negativer und rhaB-negativer Mikroorganismus
WO2018045184A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Engineered bacteria secreting therapeutic proteins and methods of use thereof
CN109576198B (zh) * 2018-11-09 2022-06-07 南京工业大学 一株敲除fimA基因的重组大肠杆菌及其构建方法与应用
US20230287438A1 (en) * 2020-09-15 2023-09-14 Northeastern University Plasmid vectors for in vivo selection-free use with the probiotic e. coli nissle

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19713543B4 (de) * 1997-04-02 2007-01-11 Pharma-Zentrale Gmbh Bakterielle Plasmide
DE19751242C2 (de) 1997-11-19 2001-02-08 Pharma Zentrale Gmbh DNA-Sequenzen aus Fimbriengenen von Escherichia coli Stamm DSM 6601

Also Published As

Publication number Publication date
NO20002550D0 (no) 2000-05-18
PL340330A1 (en) 2001-01-29
WO1999025870A1 (de) 1999-05-27
EP1038035A1 (de) 2000-09-27
NO20002550L (no) 2000-07-18
CZ289739B6 (cs) 2002-03-13
JP2004516801A (ja) 2004-06-10
EE200000231A (et) 2001-06-15
PL190847B1 (pl) 2006-02-28
US6489107B1 (en) 2002-12-03
CZ20001873A3 (cs) 2000-10-11
HUP0004409A3 (en) 2001-09-28

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Legal Events

Date Code Title Description
FC4A Lapse of provisional application due to refusal