HUP0003728A2 - Új poliketidek előállítására szolgáló módszer - Google Patents

Új poliketidek előállítására szolgáló módszer

Info

Publication number
HUP0003728A2
HUP0003728A2 HU0003728A HUP0003728A HUP0003728A2 HU P0003728 A2 HUP0003728 A2 HU P0003728A2 HU 0003728 A HU0003728 A HU 0003728A HU P0003728 A HUP0003728 A HU P0003728A HU P0003728 A2 HUP0003728 A2 HU P0003728A2
Authority
HU
Hungary
Prior art keywords
diketide
generate
produce novel
polyketides
modified pks
Prior art date
Application number
HU0003728A
Other languages
English (en)
Inventor
David E. Cane
Chaitan Khosla
Guanglin Luo
Rembert Pieper
Original Assignee
Kosan Biosciences Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kosan Biosciences Inc. filed Critical Kosan Biosciences Inc.
Publication of HUP0003728A2 publication Critical patent/HUP0003728A2/hu

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H17/00—Compounds containing heterocyclic radicals directly attached to hetero atoms of saccharide radicals
    • C07H17/04—Heterocyclic radicals containing only oxygen as ring hetero atoms
    • C07H17/08—Hetero rings containing eight or more ring members, e.g. erythromycins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/08—Oxygen as only ring hetero atoms containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A jelen találmány módosított PKS génklasztereket ír le, melyek újpoliketideket hoznak létre egy hatékony rendszerben egy gazdasejtbenvagy egy sejtmentes extraktumban. Az új poliketidek az R1-CHOHCHR2COA(1) vagy R1CH=CR2COA (2) általános képlettel rendelkező diketidekbeépülésével hozhatók létre. Az (1) és (2) általános képletben A egy adiketidet aktiváló egységet jelent, és R1 és R2 közül legalább azegyik a módosított PKS klaszter által norumálisan feldolgozottdiketidben természetesen előforduló egység helyetti szubsztituens. Apoliketidek kezelhetők a S. erythrea tenyészeteivel, a metilált,hidroxilált és glikozilált formák létrehozása céljából. Ó
HU0003728A 1997-07-17 1998-07-17 Új poliketidek előállítására szolgáló módszer HUP0003728A2 (hu)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/896,323 US6066721A (en) 1995-07-06 1997-07-17 Method to produce novel polyketides

Publications (1)

Publication Number Publication Date
HUP0003728A2 true HUP0003728A2 (hu) 2001-02-28

Family

ID=25406015

Family Applications (1)

Application Number Title Priority Date Filing Date
HU0003728A HUP0003728A2 (hu) 1997-07-17 1998-07-17 Új poliketidek előállítására szolgáló módszer

Country Status (13)

Country Link
US (3) US6066721A (hu)
EP (1) EP0996714A2 (hu)
JP (1) JP2001510038A (hu)
KR (1) KR20010021933A (hu)
CN (1) CN1267332A (hu)
AU (1) AU736972B2 (hu)
BR (1) BR9813004A (hu)
CA (1) CA2296728A1 (hu)
HU (1) HUP0003728A2 (hu)
IL (1) IL134074A0 (hu)
MX (1) MXPA00000623A (hu)
NZ (1) NZ502360A (hu)
WO (1) WO1999003986A2 (hu)

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US5712146A (en) * 1993-09-20 1998-01-27 The Leland Stanford Junior University Recombinant combinatorial genetic library for the production of novel polyketides
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US6558942B1 (en) * 1994-05-06 2003-05-06 The Leland Stanford Junior University Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
US6066721A (en) * 1995-07-06 2000-05-23 Stanford University Method to produce novel polyketides
US6927057B2 (en) 1993-09-20 2005-08-09 Kosan Biosciences Macrolide analogs
US20030170725A1 (en) * 1993-09-20 2003-09-11 Chaitan Khosla Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
US6495348B1 (en) 1993-10-07 2002-12-17 Regents Of The University Of Minnesota Mitomycin biosynthetic gene cluster
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US6399789B1 (en) * 1996-12-18 2002-06-04 Kosan Biosciences, Inc. Multi-plasmid method for preparing large libraries of polyketides and non-ribosomal peptides
US20040209322A1 (en) * 1997-04-30 2004-10-21 Chaitan Khosla Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
US20080003648A1 (en) * 1997-04-30 2008-01-03 Chaitan Khosla Method to prepare macrolide analogs
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US6410301B1 (en) 1998-11-20 2002-06-25 Kosan Biosciences, Inc. Myxococcus host cells for the production of epothilones
KR20070087132A (ko) 1998-11-20 2007-08-27 코산 바이오사이언시즈, 인코포레이티드 에포틸론 및 에포틸론 유도체의 생산을 위한 재조합 방법및 물질
EP1754700A3 (en) 1999-01-27 2007-05-09 Kosan Biosciences, Inc. Synthesis of oligoketides
CA2361040A1 (en) 1999-01-27 2000-08-03 Kosan Biosciences, Inc. Synthesis of oligoketides
JP2002536014A (ja) 1999-02-09 2002-10-29 ザ、ボード、オブ、トラスティーズ、オブ、ザ、リーランド、スタンフォード、ジュニア、ユニバーシティ ポリケチドシンターゼモジュールの有効性を媒介する方法
US7001748B2 (en) * 1999-02-09 2006-02-21 The Board Of Trustees Of The Leland Stanford Junior University Methods of making polyketides using hybrid polyketide synthases
MXPA01010521A (es) 1999-04-16 2003-08-19 Johnson & Johnson Antibacteriales de cetolidos.
US6451768B1 (en) 1999-04-16 2002-09-17 Kosan Biosciences, Inc. Macrolide antiinfective agents
US6514944B2 (en) 1999-04-16 2003-02-04 Kosan Biosciences, Inc. Macrolide antiinfective agents
CN1241931C (zh) 1999-04-16 2006-02-15 高山生物科学股份有限公司 大环内酯抗感染药物
US6590083B1 (en) 1999-04-16 2003-07-08 Ortho-Mcneil Pharmaceutical, Inc. Ketolide antibacterials
US6939861B2 (en) 1999-04-16 2005-09-06 Kosan Biosciences, Inc. Amido macrolides
EP1212331B1 (en) * 1999-08-24 2004-04-21 Ariad Gene Therapeutics, Inc. 28-epirapalogs
US7067526B1 (en) 1999-08-24 2006-06-27 Ariad Gene Therapeutics, Inc. 28-epirapalogs
US6524841B1 (en) 1999-10-08 2003-02-25 Kosan Biosciences, Inc. Recombinant megalomicin biosynthetic genes and uses thereof
US7033818B2 (en) * 1999-10-08 2006-04-25 Kosan Biosciences, Inc. Recombinant polyketide synthase genes
EP2135955B1 (en) * 1999-10-13 2013-07-31 The Board of Trustees of The Leland S. Stanford Junior University Biosynthesis of polyketide synthase substrates
US6939691B1 (en) 1999-10-13 2005-09-06 Board Of Trustees Of The Leland Stanford Junior University E. coli and Streptomyces host cells that contain MatBC genes or E. coli host cells that contain pcc genes useful for enhanced polyketide production
DE60026563D1 (de) 1999-10-25 2006-05-04 Kosan Biosciences Inc Herstellung von polyketiden
EP1244800B1 (en) * 1999-10-29 2007-03-14 Kosan Biosciences, Inc. Rapamycin analogs
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CA2348145C (en) * 2001-05-22 2005-04-12 Surface Engineered Products Corporation Protective system for high temperature metal alloys
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US20030077759A1 (en) * 2000-12-28 2003-04-24 Zhihao Hu Plasmids for polyketide production
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ATE417856T1 (de) * 2002-08-29 2009-01-15 Pfizer Motilidverbindungen
AU2003277149A1 (en) * 2002-09-26 2004-04-19 Kosan Biosciences, Inc. Synthetic genes
WO2004029224A2 (en) * 2002-09-30 2004-04-08 Compound Therapeutics, Inc. Methods of engineering spatially conserved motifs in polypeptides
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Also Published As

Publication number Publication date
EP0996714A2 (en) 2000-05-03
KR20010021933A (ko) 2001-03-15
NZ502360A (en) 2001-11-30
US6261816B1 (en) 2001-07-17
MXPA00000623A (es) 2002-04-24
US20010024811A1 (en) 2001-09-27
WO1999003986A3 (en) 1999-04-08
AU8412398A (en) 1999-02-10
AU736972B2 (en) 2001-08-09
CA2296728A1 (en) 1999-01-28
US6066721A (en) 2000-05-23
IL134074A0 (en) 2001-04-30
CN1267332A (zh) 2000-09-20
WO1999003986A2 (en) 1999-01-28
BR9813004A (pt) 2002-02-05
JP2001510038A (ja) 2001-07-31

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