HUE032752T2 - Eljárás L-valin elõállítására propionáttal indukálható ilvBN-operont tartalmazó rekombináns corynebacteriumok alkalmazásával - Google Patents
Eljárás L-valin elõállítására propionáttal indukálható ilvBN-operont tartalmazó rekombináns corynebacteriumok alkalmazásával Download PDFInfo
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- HUE032752T2 HUE032752T2 HUE13170248A HUE13170248A HUE032752T2 HU E032752 T2 HUE032752 T2 HU E032752T2 HU E13170248 A HUE13170248 A HU E13170248A HU E13170248 A HUE13170248 A HU E13170248A HU E032752 T2 HUE032752 T2 HU E032752T2
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12P7/00—Preparation of oxygen-containing organic compounds
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- C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
- C12Y202/01—Transketolases and transaldolases (2.2.1)
- C12Y202/01006—Acetolactate synthase (2.2.1.6)
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- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/03—Oxo-acid-lyases (4.1.3)
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Claims (5)
- ELJÁRÁS L-VAÍJN &LOÁLLO'AsARA FROSNONkATTAL INÖNKÁULATÓ iívI^-OFEHONT TARTALMAZÓ rii-KOMFiiNÁNEE;OR¥NEBACTERRSÁ!OK ALRáLSIA;áÁSÁVAt, S:»MMSd»!mngé»y|Kmtofi L l |»o»\k \ v«t* Λ siLmo«o ϊ * *>« ttesvsds%bs ΪΜ#Μ to«JAá!Ms»l amely saríalroagga a kdveSkeAAd; operátor aki svstású pnKssákkotkkR arsíí1>;5ís· sgek^sxSása legalább SS%-batt aasttos a SEQ 1D NO: L.
- 2 vagy 3 ágsÓBís ssekvettcsák !--12). ixttiiciölítök s7ekvendéksYid; amelyhsg 4:Εφ,& skbyáb>r kblMlk. és amely alán a 3' vegén így promólemktívltáaü második poldukkood, vsiásxbií. 3<ádülskiáFsgimá2'a?egyaeg.«' kas kódoló ílvB- és bvN-géssek vasmak ttkikSdókópesett ebrnk-gvig Isisms sgabályoaga sg HvBN-géaek msn»gknpd<gás a P?pR-aktÍYSOx kagcYídskkhásuk ibggvésmb>e;i< cávatt tápkdíegbssy asssalyks; egy éhé lesig ás bsbokáíólgök nellak bekövetkeab rdvekedéd tágss Idád egy má-xedák ilglsias Ittdiikálóaserkónl: pmpkxub vágy ;2-íSí«ld-kiÍsi kk*ti! hozzáadásra, snittek sóséit l.-váid sdsise-sMíédik dysm késlbstsóssvek kdz5U:. assselyekbess óaáilss:b#s3*ádók fai a sápkoseghex edvagy a «.egekben.
- 3, Μ I Igdsypöfss *gs?ísaj· aljáiig ásszal jellemezve, kegy aa öpcsdksráklsssdsö ps^íirsaklatttssl iaAáfSiág agy í.JR )”} ttokrttíkfeoSkkst assíslysiek ásek^ssdsttk: tegs^ábfe fö^k-bs-s a&mss á EBi| 113 NO, I, 3 vagy a ., ! i ' SÍ ,nkv> ttv s,VK^Sv,'s<'! v·*^ iA - JV o » vhsAv\ Sv : dOH-ban asmms á Sklg SD NO k 3 vagy 3 sseds-tl 7T-IÖ5. pozldék szerbéi szekvetsdávak > Az L vágy 2. sgédypöös: s.zend:i epüh, azzsd jetaezve, hogy az ssparásosiíkdvkásd pollraskieesld blyai&jsó sd»kics>ss;k axnelypék SAVhv^fscisijá kps!|bb 90%-hsm esosms á SEQ 10 .NO; í> * '*>gy AA^^RiMAb pv. >u«.A \ 5. s eedghvsl
- 4, Az előzd sgcttvpv>;ás5k agyske sgesthíl ssjáiás, *a**í. hvgy δ pfomotesáfctsystskv»! poiittokhedd oiy&o polka; kle sokk asr-dynek yzekvesK.Nja legalább bCAk-bán azonos a Si'ó
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Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| EP13170248.2A EP2811028B1 (de) | 2013-06-03 | 2013-06-03 | Verfahren zur Herstellung von L-Valin unter Verwendung rekombinanter Corynebakterien enthaltend das durch Propionat induzierbare ilvBN-Operon |
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| HUE032752T2 true HUE032752T2 (hu) | 2017-10-30 |
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| HUE13170248A HUE032752T2 (hu) | 2013-06-03 | 2013-06-03 | Eljárás L-valin elõállítására propionáttal indukálható ilvBN-operont tartalmazó rekombináns corynebacteriumok alkalmazásával |
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| Country | Link |
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| US (1) | US10113190B2 (hu) |
| EP (1) | EP2811028B1 (hu) |
| JP (1) | JP2016519949A (hu) |
| KR (1) | KR20160015298A (hu) |
| CN (1) | CN105492616A (hu) |
| BR (1) | BR112015029985A2 (hu) |
| DK (1) | DK2811028T3 (hu) |
| ES (1) | ES2623161T3 (hu) |
| HU (1) | HUE032752T2 (hu) |
| PL (1) | PL2811028T3 (hu) |
| RU (1) | RU2675506C2 (hu) |
| WO (1) | WO2014195154A1 (hu) |
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| CN106701853B (zh) * | 2016-12-02 | 2019-09-20 | 武汉远大弘元股份有限公司 | 一种产l-异亮氨酸谷氨酸棒杆菌发酵培养基及培养方法 |
| EP3415622A1 (en) | 2017-06-14 | 2018-12-19 | Evonik Degussa GmbH | Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases |
| US11021697B2 (en) * | 2017-07-11 | 2021-06-01 | Cj Cheiljedang Corporation | Acetohydroxy acid synthase variant, microorganism comprising the same, and method of producing L-branched-chain amino acid using the same |
| KR101996129B1 (ko) | 2017-07-11 | 2019-07-04 | 씨제이제일제당 (주) | 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법 |
| EP3456833A1 (en) | 2017-09-18 | 2019-03-20 | Evonik Degussa GmbH | Method for the fermentative production of l-amino acids |
| CA3076745A1 (en) * | 2017-10-02 | 2019-04-11 | Metabolic Explorer | Method for producing organic acid salts from fermentation broth |
| EP3467099A1 (en) | 2017-10-05 | 2019-04-10 | Evonik Degussa GmbH | Method for the fermentative production of l-amino acids |
| CN108753810B (zh) * | 2018-05-22 | 2021-06-18 | 昆明理工大学 | 一种转录调节蛋白基因orf2的用途 |
| KR102134418B1 (ko) * | 2019-06-17 | 2020-07-16 | 씨제이제일제당 주식회사 | L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법 |
| WO2021037190A1 (zh) * | 2019-08-29 | 2021-03-04 | 天津科技大学 | 一种2-异丙基苹果酸合成酶及其工程菌与应用 |
| KR102153534B1 (ko) * | 2019-09-02 | 2020-09-09 | 씨제이제일제당 주식회사 | 신규한 프로모터 및 이를 이용한 아미노산 생산 방법 |
| CN110551772B (zh) * | 2019-09-21 | 2023-03-28 | 新疆阜丰生物科技有限公司 | 一种提高l-异亮氨酸产量的方法 |
| KR102147381B1 (ko) * | 2019-11-22 | 2020-08-24 | 씨제이제일제당 주식회사 | 아세토하이드록시산 신타제 신규 변이체 및 이를 포함하는 미생물 |
| CN111607623B (zh) * | 2020-05-29 | 2022-02-11 | 江南大学 | 一种代谢工程改造大肠杆菌制备α-酮异戊酸的方法 |
| RU2753996C1 (ru) * | 2020-10-05 | 2021-08-25 | Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт" - ГосНИИгенетика) | Бактерия Corynebacterium glutamicum с повышенной способностью продуцировать L-валин и способ получения L-валина с использованием этой бактерии |
| KR102464883B1 (ko) | 2020-12-11 | 2022-11-09 | 씨제이제일제당 주식회사 | 신규한 감마-아미노부티르산 퍼미에이즈 변이체 및 이를 이용한 이소류신 생산 방법 |
| KR102470602B1 (ko) | 2020-12-11 | 2022-11-25 | 씨제이제일제당 주식회사 | 신규한 분지 연쇄 아미노산 아미노트렌스퍼라아제 변이체 및 이를 이용한 이소류신 생산 방법 |
| CN117106042A (zh) * | 2021-08-23 | 2023-11-24 | 黑龙江伊品生物科技有限公司 | Yh66-rs07020突变体蛋白及其相关生物材料在制备缬氨酸中的应用 |
| KR20230045990A (ko) | 2021-09-29 | 2023-04-05 | 씨제이제일제당 (주) | 신규한 아세토하이드록시산 신테아제 변이체 및 이를 이용한 l-이소류신 생산방법 |
| KR102673796B1 (ko) | 2021-09-29 | 2024-06-10 | 씨제이제일제당 주식회사 | 신규한 아세토하이드록시산 신테아제 변이체 및 이를 이용한 l-이소류신 생산방법 |
| KR20230094761A (ko) | 2021-12-21 | 2023-06-28 | 씨제이제일제당 (주) | L-이소루신 생산 미생물 및 이를 이용한 l-이소루신 생산 방법 |
| KR20240105550A (ko) * | 2022-12-28 | 2024-07-08 | 씨제이제일제당 (주) | 신규한 아세토락테이트 합성효소 활성을 갖는 변이체 폴리펩티드 및 이를 이용한 l-글루탐산 생산 방법 |
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-
2013
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- 2013-06-03 DK DK13170248.2T patent/DK2811028T3/en active
- 2013-06-03 ES ES13170248.2T patent/ES2623161T3/es active Active
- 2013-06-03 EP EP13170248.2A patent/EP2811028B1/de not_active Not-in-force
- 2013-06-03 HU HUE13170248A patent/HUE032752T2/hu unknown
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2014
- 2014-05-23 BR BR112015029985A patent/BR112015029985A2/pt not_active IP Right Cessation
- 2014-05-23 CN CN201480043905.5A patent/CN105492616A/zh active Pending
- 2014-05-23 RU RU2015156852A patent/RU2675506C2/ru not_active IP Right Cessation
- 2014-05-23 KR KR1020157036963A patent/KR20160015298A/ko active IP Right Grant
- 2014-05-23 US US14/894,513 patent/US10113190B2/en not_active Expired - Fee Related
- 2014-05-23 JP JP2016515744A patent/JP2016519949A/ja active Pending
- 2014-05-23 WO PCT/EP2014/060680 patent/WO2014195154A1/de active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| US20160115506A1 (en) | 2016-04-28 |
| KR20160015298A (ko) | 2016-02-12 |
| EP2811028B1 (de) | 2017-02-01 |
| DK2811028T3 (en) | 2017-05-01 |
| WO2014195154A1 (de) | 2014-12-11 |
| US10113190B2 (en) | 2018-10-30 |
| EP2811028A1 (de) | 2014-12-10 |
| RU2015156852A (ru) | 2017-07-17 |
| JP2016519949A (ja) | 2016-07-11 |
| BR112015029985A2 (pt) | 2017-09-26 |
| PL2811028T3 (pl) | 2017-07-31 |
| RU2675506C2 (ru) | 2018-12-20 |
| CN105492616A (zh) | 2016-04-13 |
| RU2015156852A3 (hu) | 2018-04-02 |
| ES2623161T3 (es) | 2017-07-10 |
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