ES2545683A1 - Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero. - Google Patents

Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero. Download PDF

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Publication number
ES2545683A1
ES2545683A1 ES201430173A ES201430173A ES2545683A1 ES 2545683 A1 ES2545683 A1 ES 2545683A1 ES 201430173 A ES201430173 A ES 201430173A ES 201430173 A ES201430173 A ES 201430173A ES 2545683 A1 ES2545683 A1 ES 2545683A1
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Prior art keywords
protein
new
gene
cry
hemiptera
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Granted
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ES201430173A
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ES2545683B1 (es
Inventor
Primitivo Caballero Murillo
Leopoldo PALMA DOVIS
Iñigo Ruíz De ESCUDERO FUENTEMILLA
Delia Muñoz Labiano
Jesús MURILLO MARTINEZ
Colin Berry
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Universidad Publica de Navarra
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Consejo Superior de Investigaciones Cientificas CSIC
Universidad Publica de Navarra
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Priority to ES201430173A priority Critical patent/ES2545683B1/es
Priority to PCT/ES2015/070083 priority patent/WO2015118207A1/es
Publication of ES2545683A1 publication Critical patent/ES2545683A1/es
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C07K14/325—Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
    • A01N37/46—N-acyl derivatives
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Insects & Arthropods (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero. La invención se refiere a un nuevo gen que codifica una proteína Cry (o delta-endotoxina), a la propia proteína codificada por dicho gen, a los sistemas para la expresión de la misma (vectores y células hospedadoras), y a la utilización de dicho gen y/o dicha proteína para combatir plagas de hemípteros, con preferencia por el pulgón verde, Myzus persicae, tanto mediante el uso de una composición que contenga la nueva proteína y/o un sistema para su expresión, como mediante plantas transgénicas en las que se expresa el gen. Dichas composiciones y plantas transgénicas son de especial interés, pues la proteína nueva proteína Cry presenta una capacidad insecticida con Myzus persicae especialmente buena, en comparación con otras proteínas Cry conocidas, que presentaban una capacidad insecticida reducida contra áfidos y no se conocía ninguna activa con Myzus persicae.

Description

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ES201430173A 2014-02-10 2014-02-10 Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero. Active ES2545683B1 (es)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ES201430173A ES2545683B1 (es) 2014-02-10 2014-02-10 Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero.
PCT/ES2015/070083 WO2015118207A1 (es) 2014-02-10 2015-02-10 Nueva proteína cry de bacillus thuringiensis con actividad insecticida para combatir un hemíptero

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201430173A ES2545683B1 (es) 2014-02-10 2014-02-10 Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero.

Publications (2)

Publication Number Publication Date
ES2545683A1 true ES2545683A1 (es) 2015-09-14
ES2545683B1 ES2545683B1 (es) 2016-09-06

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ES201430173A Active ES2545683B1 (es) 2014-02-10 2014-02-10 Nueva proteína Cry de Bacillus thuringiensis con actividad insecticida para combatir un hemíptero.

Country Status (2)

Country Link
ES (1) ES2545683B1 (es)
WO (1) WO2015118207A1 (es)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719839A (zh) * 2016-12-03 2017-05-31 青岛漾花湖农业科技有限公司 一种预防大棚西瓜苗重茬根腐病的益生菌菌剂制备
CN107828817A (zh) * 2017-09-29 2018-03-23 杭州瑞丰生物科技有限公司 一种利用Bt蛋白防治农作物半翅目害虫的方法
CN113180046A (zh) * 2021-03-31 2021-07-30 中国农业科学院植物保护研究所 杀虫蛋白在防治蚜虫中的应用

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110066322B (zh) * 2019-04-04 2020-09-29 四川农业大学 一种Bt蛋白Cyt2-like及其基因和应用
CN110283833A (zh) * 2019-06-27 2019-09-27 华侨大学 cry2Ab12基因在制备抗蚜虫转基因植物的用途
CN113186339A (zh) * 2021-06-18 2021-07-30 中国农业科学院郑州果树研究所 一组用于鉴定山桃杂交群体抗/感桃蚜性状的InDel标记及其应用

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995016778A1 (en) * 1993-12-13 1995-06-22 Ecogen Inc. Control of aphids with bacillus thuringiensis toxin proteins
US6150156A (en) * 1997-02-11 2000-11-21 Calgene, Inc. Bacillus thuringiensis isolates active against sucking insects
CA3081727A1 (en) * 2009-02-27 2010-09-02 BASF Agricultural Solutions Seed US LLC Pesticidal proteins and methods for their use

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106719839A (zh) * 2016-12-03 2017-05-31 青岛漾花湖农业科技有限公司 一种预防大棚西瓜苗重茬根腐病的益生菌菌剂制备
CN107828817A (zh) * 2017-09-29 2018-03-23 杭州瑞丰生物科技有限公司 一种利用Bt蛋白防治农作物半翅目害虫的方法
CN107828817B (zh) * 2017-09-29 2021-02-19 杭州瑞丰生物科技有限公司 一种利用Bt蛋白防治农作物半翅目害虫的方法
CN113180046A (zh) * 2021-03-31 2021-07-30 中国农业科学院植物保护研究所 杀虫蛋白在防治蚜虫中的应用
CN113180046B (zh) * 2021-03-31 2022-04-12 中国农业科学院植物保护研究所 杀虫蛋白在防治蚜虫中的应用

Also Published As

Publication number Publication date
ES2545683B1 (es) 2016-09-06
WO2015118207A1 (es) 2015-08-13

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