HUE030691T2 - Eljárás nukleinsavak amplifikálására - Google Patents
Eljárás nukleinsavak amplifikálására Download PDFInfo
- Publication number
- HUE030691T2 HUE030691T2 HUE13704388A HUE13704388A HUE030691T2 HU E030691 T2 HUE030691 T2 HU E030691T2 HU E13704388 A HUE13704388 A HU E13704388A HU E13704388 A HUE13704388 A HU E13704388A HU E030691 T2 HUE030691 T2 HU E030691T2
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- HU
- Hungary
- Prior art keywords
- nanoparticles
- original
- sample
- nanoparticle
- dna
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
- B01L7/525—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones
- B01L7/5255—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples with physical movement of samples between temperature zones by moving sample containers
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y20/00—Nanooptics, e.g. quantum optics or photonic crystals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1861—Means for temperature control using radiation
- B01L2300/1872—Infrared light
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Nanotechnology (AREA)
- Optics & Photonics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Claims (5)
- I wts 'A ! A ,vtpl ikdusea SZAB ADA? Mi KA N Y ΡΟΝΤΟ K1. Eljárás nukicmsævak (1) ampli f&âlâsàra, she) a r&akOöAÄJgAm (2) naÂéiztOA kék (k) oil gonak leotidok kai (4) konjugslva gerjesztéssel tUvi&äk a bét &tkŐf$p£ót$kbe.í lemezvc, hogs a nanorészccskék (8) gerjesztésén keresztül a Ranorészééátéli l;§i iistsyezete lokált-vm }V melegsAk Ovi X* gcije\'K> iwnv< 1 urnát: úgy választjuk még,, hogy ozonna vagy rővíüehb legyen., mim a kritikus gerjesztési idő ti ::'tsl *jsi)‘íD> ahal si~ iOf, p£|i$s&ldf|g#i;nanoix\szcc.ske a\oK„, tünetes wAvs 8 \ t ag\P4ln n f ,w 2. Λ/ I igény pom ,w:nd Jura a a.vol kilemezte, htyn u niáriehtsaxakm (11 egy polimeréé iáncreakdőval ampa fik aljak. a Az előzd ménypcmtok bármely tke szeritAl hogy a «οποίο ν ce A eket t8'* lévu'd ( 10 hetijugayttk
- 4. Az előző Igénypontok bérmdyike szerinti eljárás, ázzál jellemezve, légy a natmrészecskék (&) és az oligomikleotidök (4) egyik iajta konjugátitmát előrevivő printerekhez 04), valamint rever? printerekhez (13) konjugáljuk. 5. \z a ave ev.o jvatok banneh m-, enni, elmnv a/a ai lel lernt a so, hog} a/ dsérvhan ellen-szekvenciákat (34) alkalmazunk, amelyek kénesek olyan oligonukleoíkíokhoz (4) kapcsolódni« iamelyei levalaszhníák magukat a nttm-részecskékről Őri. amelyhez korábban kapcsolódtak. Ί) Az előző igénypontok bármelyike szeriül! eljárás, azzal jellemezve, hogy a töltő mole·* kaláka? gd a nauorcszscskékhez «8) kapcsoljuk. ?.. Az. előző igénypontok. bármelyike szerinti eljárás, azzal jellemezve, hogy M rtarvrés/eeskéken (S> levő obgonnkleoüdok\4) tartalmaznak egy térkitöltő szekvenciát (6), egy részié g.es szekvetícia fótinájában. S, A/ előző igénypontok bármelyike s/enmi eljárás. ezzel jellemezve, hogy a hő, amelyet a vavres/u shéh (8'* ge?ses.neVvd \ ívünk at e mavreszeeAA PA környezőmbe, elegendő ahhoz, lupe}· dehibridizáíjuk a nanerészeeskék iá? iclszfnén lesi' oligonukle-nidokm H) az oligamikíeotídokkal (4) liibridkálodmt nuklrinsavakről ? ; I, 9, \z dozo 'genspemox >arme!\ ke wrap daran a, ed jellemezve hogy íz - Ipar o ttod,na- egy gioKbis ndtg'.u si lepee. ΙΑ Az è!Őzé bármelyike szerinti - éljárás S; ázzál jellemezve, hogy az illesztési hőmérséklet azonos a Imsszabbodásí hőntársgk lelte I,
- 11, Az előző Igénypoötők Isázmelyike szénníl kijárás, jellemezve, hogy m ©Ijánls ideje alatt egy időpontban a Moorészeeakéknek (Ü) csak egy részéi hevitjük jetjesztessel:.
- 12, Az élező IgérApentnk Mrméiylké szerinti eljiMs< azzal jellemezve, hegy a mirhának: ( 11) a gerjesztőarmzőhőz vlszanylteit Imayltolt ttsezgatása ágy jálszöcllk le, hegy kitlőnhőző időpeet-ekhea a mlata kőlőnlxlző mszlérfogataihmt levő oőnoÁszeoskikei (I) gerjesztjük,3, Az előző : tgÉrvponlők bânpMyikè SZgrmd etj Iras, azzal jellemezéé, hogy m élj áráshatr : hőérzékeny DNS pőlteétfe ( I
- 14, Az élező igénypernek bármelyike szerinti eljárás, aezakjaliemezve:- hogy az ampáik kémás reakció temmkénok a/knneeairáeiőiél tesztprőbák 12!) Iiasználaláya! lizSárőzzak meg,
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE102012201475.6A DE102012201475B4 (de) | 2012-02-01 | 2012-02-01 | Verfahren zum Vervielfältigen von Nukleinsäuren |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HUE030691T2 true HUE030691T2 (hu) | 2017-05-29 |
Family
ID=47716001
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HUE13704388A HUE030691T2 (hu) | 2012-02-01 | 2013-02-01 | Eljárás nukleinsavak amplifikálására |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US9382583B2 (hu) |
| EP (2) | EP3101143B1 (hu) |
| JP (2) | JP2015511124A (hu) |
| CN (2) | CN104093860A (hu) |
| AU (1) | AU2013214098B2 (hu) |
| BR (1) | BR112014018737A2 (hu) |
| CA (1) | CA2862912C (hu) |
| DE (1) | DE102012201475B4 (hu) |
| DK (1) | DK2809806T3 (hu) |
| ES (1) | ES2596452T3 (hu) |
| HU (1) | HUE030691T2 (hu) |
| PL (1) | PL2809806T3 (hu) |
| RU (1) | RU2603253C2 (hu) |
| WO (1) | WO2013113910A1 (hu) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9816132B2 (en) | 2012-12-14 | 2017-11-14 | The Royal Institution For The Advancement Of Learning/Mcgill University | Heating mechanism for DNA amplification, extraction or sterilization using photo-thermal nanoparticles |
| WO2015006864A1 (en) * | 2013-07-16 | 2015-01-22 | The Royal Institution For The Advancement Of Learning/Mcgill University | Heating mechanism for dna amplification, extraction or sterilization using photo-thermal nanoparticles |
| DE102013215168B4 (de) | 2013-08-01 | 2016-01-07 | Gna Biosolutions Gmbh | Verwendung von einem oder mehreren Nanopartikeln zum Vervielfältigen von Nukleinsäuren |
| DE102013215166B3 (de) * | 2013-08-01 | 2014-10-30 | Gna Biosolutions Gmbh | PCR-Verfahren zur Superamplifikation |
| DE102013022382B9 (de) * | 2013-08-01 | 2017-11-16 | Gna Biosolutions Gmbh | Verfahren zum Vervielfältigen von Nukleinsäuren |
| JP6491240B2 (ja) * | 2014-06-18 | 2019-03-27 | ルミネックス コーポレーション | 磁気混合のための装置及び方法 |
| US10544450B2 (en) | 2014-11-07 | 2020-01-28 | Gna Biosolutions Gmbh | Method for duplicating nucleic acids by means of a polymerase chain reaction |
| WO2016074701A1 (de) | 2014-11-10 | 2016-05-19 | Gna Biosolutions Gmbh | Verfahren zum vervielfältigen von nukleinsäuren |
| EP3245283B1 (en) | 2015-01-16 | 2020-09-23 | The Regents of The University of California | Led driven plasmonic heating apparatus for nucleic acids amplification |
| CA2988553C (en) * | 2015-06-08 | 2022-02-15 | The University Of Chicago | Bipyramid-templated synthesis of monodisperse noble metal nanocrystals |
| DE102015010065B3 (de) * | 2015-08-03 | 2016-07-21 | Gna Biosolutions Gmbh | Verwendung von Nanopartikeln und von kationisch modifizierter Nukleinsäure zum Nachweis einer nachzuweisenden Nukleinsäure in einer Lösung |
| EP3184647A1 (en) * | 2015-12-23 | 2017-06-28 | Koninklijke Philips N.V. | Laser pcr and bead detection in one reaction chamber |
| DE102016120124B8 (de) | 2016-10-21 | 2018-08-23 | Gna Biosolutions Gmbh | Verfahren zum Durchführen einer Polymerase-Kettenreaktion und Vorrichtung zum Ausführen des Verfahrens |
| DE102016124692B4 (de) | 2016-12-16 | 2019-05-16 | Gna Biosolutions Gmbh | Verfahren und System zum Vervielfältigen einer Nukleinsäure |
| AU2018340884A1 (en) * | 2017-09-26 | 2020-05-07 | National Research Council Of Canada | Microfluidic assisted fabrication of polymer microparticle-metal nanoparticle composites |
| DE102017123919A1 (de) | 2017-10-13 | 2019-04-18 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Lyse von Mikroorganismen |
| CN111801429A (zh) * | 2017-11-06 | 2020-10-20 | 密歇根大学董事会 | 用于检测微生物的组合物和方法 |
| DE102018103215B3 (de) | 2018-02-13 | 2019-08-14 | Gna Biosolutions Gmbh | Verfahren und Vorrichtung zur Extraktion einer Nukleinsäure aus einer Probenflüssigkeit |
| EP3569717A3 (en) * | 2019-08-28 | 2020-04-08 | Boehringer Ingelheim Vetmedica GmbH | Diagnostic method for the detection of dna and rna viruses by carrying out a polymerase chain reaction in a heated reaction volume |
| CN112301106A (zh) * | 2020-10-30 | 2021-02-02 | 南京师范大学 | 具有光热响应的DNA修饰的金纳米颗粒的DNA-AuNP探针及其制备方法和应用 |
| CN113355455B (zh) * | 2021-05-21 | 2024-05-03 | 广东粤港澳大湾区国家纳米科技创新研究院 | 一种检测新型冠状病毒的荧光定量纳米粒子pcr的引物、引物探针组合及其应用 |
| CN113278733B (zh) * | 2021-05-21 | 2024-03-01 | 广东粤港澳大湾区国家纳米科技创新研究院 | 一种检测新冠病毒的突变毒株的引物和探针的组合 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US6953659B2 (en) * | 2000-07-14 | 2005-10-11 | Massachusetts Institute Of Technology | Direct, externally imposed control of nucleic acids |
| DE10162176B4 (de) * | 2000-12-13 | 2006-03-23 | Institut für Physikalische Hochtechnologie e.V. | Verfahren zum simultanen positionsspezifischen Schneiden von DNA |
| AU2002359510A1 (en) | 2001-11-30 | 2003-06-17 | Nanosphere, Inc. | Real-time monitoring of pcr amplification using nanoparticle probes |
| WO2005042783A1 (en) * | 2003-10-30 | 2005-05-12 | North Carolina State University | Temperature-jump enhanced electrochemical detection of nucleic acid hybridization |
| EP1840222B1 (en) * | 2004-12-29 | 2011-02-23 | Suzhou Yangtze Delta Academy of Bio-x Science Ltd. | The optimum method of amplification on polymerase chain reaction |
| US7998672B2 (en) * | 2006-05-30 | 2011-08-16 | The University Of Utah Research Foundation | Simultaneous amplification and detection of ribonucleic acid be an optical method using surface plasmon resonance |
| DE102007027654B8 (de) * | 2007-06-15 | 2012-03-15 | Gna Biosolutions Gmbh | Verfahren zum Nachweis von Nukleinsäuren |
| EP2110175A1 (de) * | 2008-03-20 | 2009-10-21 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Verfahren und Vorrichtung zur thermischen Steuerung biologischer und chemischer Reaktionen unter Einsatz von magnetischen Partikeln oder magnetischen Beads und Wechselmagnetfeldern |
-
2012
- 2012-02-01 DE DE102012201475.6A patent/DE102012201475B4/de active Active
-
2013
- 2013-02-01 JP JP2014555234A patent/JP2015511124A/ja not_active Withdrawn
- 2013-02-01 ES ES13704388.1T patent/ES2596452T3/es active Active
- 2013-02-01 EP EP16179482.1A patent/EP3101143B1/en active Active
- 2013-02-01 EP EP13704388.1A patent/EP2809806B1/en active Active
- 2013-02-01 BR BR112014018737A patent/BR112014018737A2/pt not_active Application Discontinuation
- 2013-02-01 US US14/375,946 patent/US9382583B2/en active Active
- 2013-02-01 AU AU2013214098A patent/AU2013214098B2/en not_active Ceased
- 2013-02-01 PL PL13704388T patent/PL2809806T3/pl unknown
- 2013-02-01 RU RU2014135391/10A patent/RU2603253C2/ru active
- 2013-02-01 WO PCT/EP2013/052100 patent/WO2013113910A1/en active Application Filing
- 2013-02-01 HU HUE13704388A patent/HUE030691T2/hu unknown
- 2013-02-01 CN CN201380007616.5A patent/CN104093860A/zh active Pending
- 2013-02-01 CA CA2862912A patent/CA2862912C/en active Active
- 2013-02-01 CN CN201710868078.9A patent/CN107604052A/zh active Pending
- 2013-02-01 DK DK13704388.1T patent/DK2809806T3/en active
-
2016
- 2016-01-25 JP JP2016011619A patent/JP6475645B2/ja not_active Expired - Fee Related
- 2016-06-07 US US15/175,237 patent/US9926594B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| DE102012201475B4 (de) | 2014-12-18 |
| RU2603253C2 (ru) | 2016-11-27 |
| ES2596452T3 (es) | 2017-01-09 |
| US20160281139A1 (en) | 2016-09-29 |
| CN104093860A (zh) | 2014-10-08 |
| WO2013113910A1 (en) | 2013-08-08 |
| EP2809806B1 (en) | 2016-08-10 |
| CN107604052A (zh) | 2018-01-19 |
| EP2809806A1 (en) | 2014-12-10 |
| CA2862912A1 (en) | 2013-08-08 |
| PL2809806T3 (pl) | 2017-02-28 |
| US20140377764A1 (en) | 2014-12-25 |
| AU2013214098A1 (en) | 2014-07-24 |
| US9382583B2 (en) | 2016-07-05 |
| AU2013214098B2 (en) | 2016-05-12 |
| EP3101143B1 (en) | 2018-08-01 |
| EP3101143A1 (en) | 2016-12-07 |
| BR112014018737A2 (pt) | 2017-07-04 |
| CA2862912C (en) | 2017-02-28 |
| JP2015511124A (ja) | 2015-04-16 |
| DK2809806T3 (en) | 2016-10-10 |
| JP2016127842A (ja) | 2016-07-14 |
| US9926594B2 (en) | 2018-03-27 |
| JP6475645B2 (ja) | 2019-02-27 |
| RU2014135391A (ru) | 2016-03-27 |
| DE102012201475A1 (de) | 2013-08-01 |
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