HU210984B - De 0726 variety of female inbred corn line (zea mays l.) - Google Patents

Abstract

Field of the Invention The present invention relates to an inbred maize line DE0726, which is derived from the crossing of lines 1094-H x A656, and in which the leaf recedes, late in the beginning of flowering, late appearance of the mustache, lack of anthocyanin discoloration of the chaff or very weak sealed anthocyanin ring missing at the base of the chaff, the anthocyanin discoloration of the nodules is missing or very weak, the anthocyanin discoloration of the nodes is weak, the edge of the leaf envelope is moderately hairy, the angle between the main axis of the coat of arms and the lateral branches is moderate, the lateral branch is strongly retracted, the main axis of the coat of arms is very short at the lower side and above the top side, the tube is long, the tube is medium long, its diameter is thick, its shape is slightly conical, the number of core lines is medium, the core is toothed, the color of the nucleus is yellow, the back of the nucleus is dark red and the anthocyanin coloration of the kernel is dark red and the anthocyanin coloration of the nucleus is moderate red characterized by ho the height of the plant is 190-200 cm, preferably 193.8 cm, the height of the tube is 60-70 cm, preferably 64.5 cm, the color of the root root is green, the color of the leaf after pollination is medium green, the anthocyanin coloration of the leaf envelope is strong, the leaf wind is strong the number of wrinkles in the coat of arms is preferably 6.7 cm, the number of junctions of the coat of arms is 4, the anthocyanin coloration of the pores is weak and the anthocyanin discoloration of the mustache is weak and the color of the core side is bama. EN 210 984 Description range: 8 pages (including 3 sheets)

Description

The present invention relates to a female inbred line of the breed DE0726.

Zea mays L. is a major agricultural crop in many countries around the world, especially in the USA. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. By self-pollinating or cross-pollinating F, hybrid plants produce second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the Fj hybrid, thus yielding lower and undesirable genetic characteristics. Due to the reduced characteristics resulting in a less preferred second generation F F ₂ hybrid, hybrid seeds are not collected by the growers. Each year, growers expect new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (Fj) hybrid seeds.

The production and selection of inbred lines resulting in crossing Fj hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of Fj hybrids and thus for commercialization.

Due to the important role of inbred lineages in the production of commercial Fj maize hybrids, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line DE0726.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line of the variety DE0726 which, when crossed with other inbred maize as a male or female parent unit, produces a first generation (Fj) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, an inbred corn line DE0726 is used which has high yields, excellent stems and excellent greening properties.

The inbred corn line DE0726 of the present invention can be used to produce hybrid maize, wherein a first corn line is crossed with a second corn line, wherein the first corn line or second corn line is a different inbred corn line and the other is an inbred corn line.

As mentioned above, the present invention relates to a new inbred line DE0726 which, when used as a parent line, crosses with other maize lines, preferably inbred lines, to form first generation (Fj) maize hybrids. Although the inbred line DE0726 is generally used as a female parent in crossing, it can also be used as a male parent.

DE0726 inbred corn line is a yellow horse-bred inbred line and its development is described below:

In summer 1979, we crossed line 1094-H (origin: B14 "Mt42") and line A656 (origin: B14 "Mt42) (lines 23-92 and 23-94).

In the winter of 1979, the harvested SO seeds were planted in a single row, and the Si seeds were collected and accumulated (lines 5-1397).

In the summer of 1980, SI seeds were planted in separate rows per tube. Nine S2 tubes were selected and individually stripped (lines 19,410-19,414).

In the summer of 1981, the seeds of the nine S2 tubes were planted in separate rows per tube. Three S3 tubes were selected from each of the nine rows and each was churned out (lines 20,789-20,797).

In the summer of 1982, the seeds of the three S3 tubes were planted in separate rows per tube. One S4 tube was selected from the three rows (lines 24,138-24,140).

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In the winter of 1982, the seeds of the S4 tube were planted. Six S5 tubes were selected based on the tube type and 5-1099 were individually dropped. row).

In the summer of 1983, the seeds of the six S5 tubes were planted in separate rows per tube. Five S6 tubes were selected from each of the six rows and dropped individually (lanes 10,830-10,835).

In the summer of 1984, the seeds of the five S6 tubes were planted in separate rows per tube. Three S7 tubes were selected from the six rows and 10,356-10,360 dropped each. row).

In the summer of 1985, the seeds of the three S7 tubes were planted in separate rows per tube. Three S8 tubes were selected from a row and chamfered individually (lines 9816 to 9818).

In the winter of 1985, the seeds of the three S8 tubes were planted in separate rows per tube. All S9 seeds were harvested and accumulated (lines 5-1900 through 5-1902).

In the summer of 1986, the accumulated S9 seeds were planted. A single tube was selected from line 9672 which received the FBLA code (lines 9672-9674). The line was later denoted by DE0726.

Since 1986, the pure origin of DE0726 (FBLA) has been maintained by self pollination. The line has been observed to be phenotypically and genetically stable and uniform in properties. The DE0726 (FBLA) line does not show more variability than would be expected from environmental effects.

The inbred line DE0726 is owned by Dekalb Plant Genetics and was not accessible to users.

The invention relates to cells of the inbred line of DE0726 which can be cultured in a suitable culture, then isolated and regenerated to produce the inbred line. Details of the generation process are described in C.E. Green and C.A. Rhoes, Plant Regeneration in Tissue Culture of Maize, Maize Foor Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE0726 is a yellow horse-toothed corn, which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, 12 November 1980):

Leaf, appearance (middle third of plant): drooping

Coat of arms, beginning of flowering: late

Coat of arms, anthocyanin coloration of anthers: weak

Tube, mustache appearance (the plants

50%): Late

Tube, mustache anthocyanin coloration: weak

Anthocyanin coloration of coat of arms, chaff: none or very weak

Coat of arms, anthocyanin ring at the base of the husk: none

Stems, knots anthocyanin discoloration: you are not very weak Anthocyanin coloration of stems, nodules (in the center of plant): Anthocyanin color of leaf, envelope weak (in the center of the plant): strong Leaf, sheath edge: moderately hairy Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): medium Coat of arms, appearance of side branches: strongly sloping Coat of arms, number of primary side branches: you are zero very few Coat of arms, length of crankshaft above the lowest branch: i very short Coat of arms, length of crankshaft above topmost branch: medium Plant, height (incl. Coat of arms): Pipe, where the pipe fits into long relative to total prescription height: medium Pipe, handle length: long Pipe, length: medium Tube diameter (center of tube): thick Tube Shape: Slightly conical Pipe, number of cores: Pipe, type of cores (middle of pipe) medium third): dent Tube, core tip color: yellow Tube, core backside color: dark red Tube, anthocyanin coloration of the chaff: there is Intensity of anthocyanin coloration of the tube: medium

The inbred maize line DE0726 is a yellow horse-borne inbred line derived from the 1094-H x A656 cross.

The nearest known maize line is DE0507. The inbred line DE0726 is statistically significantly different from the known inbred line DE0507 at plant height (plant DE0726 is 190 cm by 200 cm, preferably 193.8 cm, plant DE0507 is preferably 229.4 cm high) at 60 cm -70 cm, preferably 64.5 cm, and preferably 77 cm) in the number of subroutine nodes (1.8 and 0.8 on average, respectively), in the color of the subroutine (green or red), in the color of the leaf after pollination (medium green) and dark green), the anthocyanin coloration of the leaf envelope (strong or weak), the number of leafy wrinkles (few or many), the tassel peduncle length (preferably 6.7 cm and 0.9 cm), the number of tassel branches , 0 and 6.8), anthocyanin coloration of the anthers (weak or none), anthocyanin coloration of the mustache (weak or none), and the color of the nucleus side (brown, or orange).

The above characteristics of the inbred line DE0726 relative to the known inbred line DE0507 are summarized in Table 1:

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Table 7

specific DE0726 DEO5O7 THE B plant height (cm) 193.8 229.4 57.305 15.0 tube height (cm) 64.5 77.0 33.640 11.5 number of subroutine nodes 1.8 0.8 0,068 0.5 color of the side root green red leaf pollination color medium green dark green anthocyanin coloration of leaf envelope strong weak leaf leaf wrinkle few many coat of arms length (cm) 6.7 0.9 2.161 2.9 number of crest branches 4.0 6.8 1,277 2.2 anthocyanin coloration of anthers weak no anthocyanin coloration of mustache weak no core page color brown orange- yellow

A: Mean square of error

B: Mean standard deviation (significance 0.05)

The isozyme analysis of the inbred lines DE0726 and DE0507 shows three genetic differences: Got2 (2 instead of 4), B-Glul (2 instead of 7) and Phil (4 instead of 5).

The isozyme genotype of the inbred line DE0726 is compared to the known inbred line DE0507 in Table 2.

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Corn and Sorghum Industries Research Conference, 1980.

Table 2

alleles presence Place DE0726 DE0507 Acphl 4 4 Cat3 9 9 Got3 4 4 Got2 2 4 Gotl 4 4 B-Glul 2 7 Idhl 4 4 Idh2 6 6 Mdhl 6 6 Mdh2 3 3 Mdh3 16 16

alleles presence Place DE0726 DE0507 Mdh4 12 12 Mdh5 12 12 pgml 9 9 Pgm2 4 4 6 Pgdl 3.8 3.8 6 Pgd2 5 5 Phil 4 5 number of plants tested 6 6

The inbred line DE0726 is preferably used as a parent line for the production of hybrid maize, in particular first generation (Fj) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the inbred line DE0726, are placed close to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DE0726 is used as a female parent, the coat of arms is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female parent line, only the pollen from the male parent line is suitable for pollination since all the plants of the inbred line, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from female parent units are harvested to harvest seeds of the new F hybrid maize. Seeds from the male parent unit can also be harvested and used as needed, but these seeds are not suitable as hybrid seeds.

The resulting new F1 hybrid seeds can be planted next season, when high yield Fj hybrid maize plants will develop.

The inbred line DE0726 is suitable for the production of new F] maize plants which exhibit advantageous properties in which

For example, the inbreeding line DE0726 is used as a female parent line, while the inbreeding line DE6204 is used as the male parent line. The first generation (Fj) hybrid was designated DK381 (EXP138). The characteristics of the hybrid ADK381 were compared with those of the commercially available Pioneer 3902 (Helga) variant. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3 DK381 and Helga

Hybrid Fruit (bush) * Moisture content of seed (%) stem Drop (%) Roots (%) DK381 89.1 19.0 3.8 1.2 Helga 79.5 19.6 3.1 2.4 ** ** ** **

* 1 bus = 36.35 liters ** = statistically significant at 1%

It can be seen from the foregoing that the inbred line DE0726 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous properties.

Claims (1)

Patent Claim Point Inbred maize line DE0726, derived from the crossing of lines 1094-H x A656, with leaf drooping, late onset of flowering, late appearance of mustache, no or very poor anthocyanin coloration of the anvil, closed anthocyanin ring , anthocyanin coloration of nodules missing or very weak, anthocyanin coloration of nodule gaps, leafy margin moderately hairy, crest of crest axis and lateral branches moderate, lateral crest strongly above shortest, medium lateral, tube long, tube moderately long, diameter slightly conical, nucleus number medium, nucleus equine, nucleus color yellow, backside of the nucleus dark red and anthocyanin coloration of the caudate, plant height 190-200 cm, preferably 193.8 cm, tube height 60-70 cm, preferably 64.5 cm, the color of the sub-root is green, the color of the leaf after pollination is medium green, the anthocyanin coloration of the leaf envelope is strong, the tassel peduncle preferably has a length of 6.7 cm, a tibial branch number is preferably 4, the anthocyanin coloration of the anthers is poor, the anthocyanin coloration of the mustache is poor and the color of the kernel side is brown.