HU210982B - De 6207 variety of male inbred corn line (zea mays l.) - Google Patents

Abstract

Field of the Invention The invention relates to an inbred maize line DE 6207 derived from the inbred line of the variety 3737, where the leaf is retracted, the anthocyanin ring of the chaff is missing, the anthocyanin discoloration of the nodes is absent or very weak, the anthocyanin discoloration of the leaf envelope is absent or very weak, the primary side branches are missing or very weak, the primary side branches are missing or very weak. the number is thick, the diameter of the tube is thick, its shape slightly conical, the number of core lines is large, the core is yellow. A characteristic feature of the invention is that the plant is tall, the height of the tube is medium, the knots on the stem are long, the leaf coat has a mild, the coat has a long coat, the angle of the crest of the coat is medium, the number of seed rows is high, the number of nuclei is medium and the core type is medium. OQ HU 210 982 Scope of the description: 10 pages (including 3 sheets)

Description

The present invention relates to a male inbred line of the genus DE 6207.

Zea mays L., or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of hybridization, varieties with significantly improved yields, stronger stems and roots and more uniform pest and disease resistance have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hydride vitality or heterosis occurs and the hybrid plant has significantly increased yields, strong stems and roots, homogeneity, and strong resistance to pests and diseases. Self-pollination or cross-pollination of Fj hybrid plants produces second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the F 1 hybrid, thus yielding lower yields and undesirable genetic characteristics. Due to the reduced characteristics of the less preferred resulting second generation hybrids Fj F ₂ hybrid seeds not harvested by growers. On the contrary, growers expect each year new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (Fj) hybrid seeds.

The production and selection of inbred lines resulting in crossing Fj hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of F1 hybrids and thus to be commercialized.

Due to the prominent role of inbred lineages in the production of commercial Fj maize hybrids, the development of inbred lineages has received particular emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line of the variety DE 6207.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line of the genus DE 6207, which, when crossed with other inbred maize as a parent or female, produces a first generation (Fj) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, an inbred maize line DE 6207 is used which has a high yield, an excellent stem and excellent greening properties.

The inbred maize line DE 6207 of the present invention can be used to produce hybrid maize by crossing a first maize line with a second maize line, wherein the first maize line is an inbred maize line DE 6207 and the second line is an inbred maize line.

As mentioned above, the invention relates to a new inbred line of the genus DE 6207 which, when used as a parent line, can be crossed with other maize lines, preferably inbred maize lines, to form first generation (Fj) maize hybrids. Although the inbred line DE 6207 is generally used as a male parent in crossing, it can also be used as a female parent.

The inbred maize line DE 6207 is a yellow horse-bred inbred line and its development is described below:

DE 6207 was developed by self-pollination of line 3737 (owned by Pioneer Hybrid International, Des Moines, Iowa, USA) in Mason (Michigan, USA). In the summer of 1984, Pioneer 3737 was self pollinated in our owaton horticulture (Minnesota, USA). The seeds were accumulated (84 rows, 3848-Owatonna). In the summer of 1985, accumulated S1 seeds were planted in Mason (Michigan, USA). After that, summer operations were always done in Mason. 19 self-pollinated tubes were selected and individually stripped (lines 87-31 to 87-45).

In the winter of 1985-86, 19 S2 rows were planted per tube in a separate row in Kihei, Hawaii, USA. Subsequent operations were then always performed in Kihei. 32 self-pollinated tubes were selected and individually discarded.

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In the summer of 1986, 32 rows of S3 were planted in a separate row per tube. 12 self-pollinated tubes were selected and stripped individually (lines 21-43 to 21-74).

In the winter of 1986-87, 12 rows of S4 were planted in separate rows per tube. 16 self-pollinated tubes were selected and stripped individually (lines 89-151 to 89-162).

In the summer of 1987, 16 rows of S5 were planted in separate rows per tube. The S5 tube 11322 is designated DE 6207 on lines 109-47. Two self-pollinated tubes were selected from line DE 6207 and individually stripped (lines 109-45 to 109-60).

In the summer of 1988, two S6 rows were planted in separate rows per tube. After self-pollination, only rows 158-15 were harvested and the seeds were accumulated (rows 185-14-158-15).

In the winter of 1988-89, the accumulated seeds were propagated by self-pollination for the next growing season. 9 self-pollinated tubes were selected and individually stripped (lines 79-151).

In the summer of 1989, 50 rows of previously harvested 9 tubes were grown. The plants were self-pollinated and seeds from single plants of uniform rows were accumulated. The line was then assigned the international code 3IIH6 and DE 6207.

The DE 6207 line has been maintained by self-pollination and has been stable over the last 4 years. DE 6207 is uniform in all observed traits. In the coat of arms of the DE 6207, the color of the chaff and the color of the anthers are yellow at appearance. The light causes the chaff to turn purple and the ants to turn red. The coat of arms and anthers are stable in color and change reliably as the plant develops.

The inbred line DE 6207 is owned by Dekalb Plant Genetics and was not accessible to users.

The invention relates to cells of the inbred line of DE 6207, which can be cultured in a suitable culture, then isolated and regenerated to produce the inbred line. Details of the generation process are described in C.E. Green and C.A. Rhodes, Plant Regeneraton in Tissue Culture of Maze, Maize Foor Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 6207 is a yellow horse-toothed corn, which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, 12 November 1980):

Leaf, appearance (middle third of plant): drooping

Coat of arms, beginning of flowering: late

Coat of arms, anthocyanin coloration of anthers: none or very weak

Tube, hair appearance (50% of plants): late

Tube, hair anthocyanin coloration: medium

Coat of Arms, Chestnut Anthocyanin Color: Medium Coat of Arms, Anthocyanin Ring at Chestnut Root: Missing Stem, Lump Anthocyanin: None or Very Poor

Anthocyanin coloration of stems, nodules (in the center of the plant): none or very weak

Anthocyanin color of leaf, envelope (center of plant): none or very weak

Leaf, hairs on the brim: weak

Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): medium

Coat of arms, appearance of side branches: slightly bending Coat of arms, number of primary branches: medium

Coat of arms, length of crankshaft above lowermost side: medium

Coat of arms, length of crankshaft above topmost branch: long

Plant height (including tassel): long

Pipe, where the tube fits relative to the total height of the plant: medium

Tube, handle length: short

Tube, length: long

Tube diameter (center of tube): Thick

Tube Shape: Slightly conical

Pipe, number of cores: many

Tube, core type (middle third of tube): medium

Tube color, yellow color

Tube, core backside color: yellow-orange

Tube, anthocyanin coloration of the chaff: yes

Tube, anthocyanin coloration of the chaff: medium

The inbred maize line DE 6207 is a yellow horse-bred inbred line derived from the Pioneer breed 3737. The closest known maize line is DE 8016. The inbred line DE 6207 is statistically significantly different from the known inbred line DE 8016 at the height of the plant (198 cm and 160.7 cm, respectively) and at the height of the tube (71 cm and

52.5 cm), the number of nodules with the root (0.2 and 1.2), the color of the root (red or green), the length of the nodules on the stem (13 and 10.0 cm, respectively), the hairiness of the sheath ( mild to medium), longitudinal wavy (many or few), tassel length (38.0 cm or

30.5 cm), the tassel in the length of the ear (27.0 cm, respectively)

20.5 cm) in coat length (6.0 cm and 9.4 cm, respectively), in appearance of the coat of arms (compact or open, in coat angles (medium or open), in the color of anthers (greenish yellow or red) , the color of the coat of arms (red or green), the length of the tube (16.6 cm and 13.8 cm), the length of the tube handle (11.4 cm and 16.4 cm), the number of cores (24, 3), in the type of seed (medium or equine),

HU 210 982 B in color of the side of the core (deep yellow or orange), time to 50% moisture content of the pollen (growth units) (1375 and 1332) and hair appearance time (growth units) (1369 and 1343, respectively).

The above characteristics of the DE 6207 inbred line relative to the known DE 8016 inbred line are summarized in Table 1:

DE 6207 is similar to DE 8085. At maturity, it is much closer to the DE 8085 variety line than to the DE 8016 variety line. The DE 6207 variety line is significantly different from the known DE 8085 line in terms of shoot height, shoot color, tube height and many other characteristics. Some differences are noted in Table 1.

The habituation and production of the inbred line of DE 8016 are shown in Figures 1, 3 and 5 and the habituation and production of the inbred line of DE 6207 are shown in Figures 2, 4 and 6.

Table 1

specific DE 6207 DE 8016 DE 8085 THE B shoot height (cm) 24 26 32 3.51 6.9 shoot color dark dark clear plant height (cm) 198 160.7 194.8 5.43 10.7 tube height (cm) 71 52.5 90.3 5.84 11.6 subroutine knot 0.2 1.2 0.7 0.25 0.5 color of the side root red green green knot length (cm) 13 10.0 11.7 1.11 2.2 hair on the sheath mild medium mild longitudinal waviness many few no coat of arms length (cm) 38.0 30.5 29.5 2.04 4.0 coat of arms length (cm) 27.0 20.5 21.0 1.25 2.5 coat of arms length (cm) 6.0 9.4 3.5 1.02 2.0 appearance of coat of arms compact open open crest of branches medium open open number of crest branches 10.1 10.1 7.0 1.06 2.1 coat of arms color greenish yellow red red coat of arms color of chaff red green green tube hair color red red pink number of pipes 1.5 1.7 U 0.16 0.3 tube length (cm) 16.6 13.8 14.2 0.72 1.4 tube handle length (cm) 11.4 16.1 13.2 1.2 2.5 number of cores 15 24.3 28.0 1.57 3.1 seed type medium dent dent core side color deep yellow n.sárga n.sárga pollen 50% moisture content (unit) 1375 1332 1358 50% hair appearance (unit) 1369 1343 1345

According to the isozyme analysis of inbred lines of DE 6207 and DE 8016 there are two genetic differences: Acphl (2 and 4) and Got2 (4 and 2), respectively.

The isozyme genotype of the inbred line DE 6207 is compared to the known inbred line DE 8016 in Table 2.

Isozyme analysis of DE 6207 and DE 8085 shows no genetic difference.

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Corn and Sorghum Industries Research Conference, 1980.

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Table 2

Allelomorph presence Place DE 6207 DE 8016 DE 8085 Acphl 2 4 2 Cat3 9 9 9 Got3 4 4 4 Got2 4 2 4 Gotl 4 4 4 Idhl 4 4 4 Idh2 6 6 6 Mdhl 6 6 6 Mdh2 3.5 3.5 3.5 Mdh3 16 16 16 Mdh4 12 12 12 Mdh5 12 12 12 pgml 9 9 9 Pgm2 4 4 4 6 Pgdl 3.8 3.8 3.8 6 Pgd2 5 5 5 Phi 4 4 4 number of plants tested 6 24 42

As the parent line of the inbred line of the genus DE 6207, it is advantageous to produce hybrid maize, especially first generation (Fj) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the inbred line DE 6207, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or agrochemicals for proper development.

During flowering, if the inbred line DE 6207 is used as a parent, the coat of arms of the other parent is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated, while grasses usually cross-pollinate due to the wind. As a result of the neutering of the female parent line, only pollen from the male parent line is suitable for pollination, since all the plants of the inbred line that carry pollen female role in hybridization was removed.

During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from the female parent units are harvested to harvest the seeds of the new Fj hybrid maize. Seeds from the male parent unit may also be harvested and used as needed, but these seeds may not be used as hybrid seeds.

The resulting new F, hybrid seeds can be planted next season, when high yield Fj hybrid maize plants will develop.

The inbred line De 6207 is suitable for the production of novel maize plants having advantageous properties, for example, the inbred line DE 6207 is used as a male parent line and DE 0726 is an inbred line as female parent. The first generation (Fj) hybrid received the DKEXP127 signal. The characteristics of the DKEXP137 hybrid were compared with those of the commercially available Pioneer 3902 variant. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3

DKEXP137 and Pioneer 3902

Hybrid Space- the m- (Bu) Nuclear wetting ves- ness reserve lom (%) Flower- tion time (one- ity) Woman- receipt today- Gas- pany (Cm) Tube today- Gas- pany (Cm) Shit- roll (%) roots kér- roll (%) EXP137 90.3 19.3 707 241.9 106.3 3.9 1.4 3902 81.5 19.9 645 218.4 96.7 3.1 2.0 XX XX XX XX XX XX XX

number of places: 213 x statistically significant at 5% xx statistically significant at 1% + statistically significant at 10%

It can be seen from the foregoing that the inbred line of the DE 6207 variety provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with special and advantageous properties. Thus, the DKEXP137 hybrid has the advantage of high yield, low moisture yield, and reduced rooting.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that the present invention,

GB 210 982 különböző Various changes and modifications are possible.

Claims (3)

Patent Claim Point DE 6207 inbred maize line derived from inbred line 3737 with deflection of leaf, lack of anthocyanic ring of pod, lack or very low anthocyanin coloration of nodules, lack of anthocyanin, very slight, the diameter of the tube is thick, its shape is slightly conical and the number of cores is numerous, 5 the apex of the nucleus is yellow and the anthocyanin coloration of the tube is medium, characterized by the plant being high, the tube having a medium height, the knots on the stalk being long, the hair coat is light, the coat of arms is long, the angles 10 is a lot, the seed type is medium.