HU206018B - Male inbred maize line of de 0716 sign - Google Patents

Abstract

FIELD OF THE INVENTION The present invention relates to a male bovine maize line DE 0716 derived from the cross (4744xMO17) xMO17 and which is late in the flowering, the anthocyanin discolouration of the pores is missing or very weak, the anthocyanin discolouration of the nodules is absent at the base of the chaff. or very weak, the anthocyanin discoloration of the nodes is weak, the anthocyanin discoloration of the leaf envelope is poor, the edge of the envelope is weakly hairy, the number of primary side branches in the coat of arms is low, the length of the crankshaft is moderate over the lower side, the tube's length is medium, the tube's length is medium, the tube is long and the tube is long and slightly tube conical, medium in diameter, few in number, core type: horsehair and orange in back. A characteristic feature of the invention is that the anthocyanin discoloration of the mustache is weak, the angle between the main axis of the coat of arms and the lateral side of the coat is small, the height of the plant is 210-218 cm, preferably 214 cm, and the height of the tube is 90-100 cm, preferably 95 cm, the tip of the core yellow and chalk anthocyanin discoloration is moderate. fc's t · - / Λ, ο «· / - • Ski <fc α» ϊϊ <* **** · © »rí t · CQ i'SeSÍ'1 *» «ffl? ■ *> »** ·» ■ * •?? 0716 · ΜΟ17ΗΤ EN 206 018 l. Scope of the description: 8 pages (including 2 sheets)

Description

The present invention relates to a male inbred maize line DE 0716.

Zea mays L is a major agricultural crop in many countries around the world, especially in the USA. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of hybridization, varieties with significantly improved yields, stronger stems and roots and more uniform insect and disease resistance have been bred.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. Homozygosity can be achieved by repeated self-fertilization within the inbred line, and generally the sixth or seventh generation by self-pollination is considered to be genetically pure.

However, inbreeding for genetic identity leads to a decrease in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding and breeding experiments in maize cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. By self-pollinating or cross-pollinating F, hybrid plants produce second-generation (F ₂ ) hybrids. The F ₂ hybrid plants and seeds properties less favorable than the D (hybrid properties, thus the yield is lower and undesirable genetic characteristics get phrase Due to the reduced characteristics resulting in a less preferred second generation F ₂ hybrid F |. Hybrids, seeds are not collected by the breeders Each year growers expect new hybrid seeds obtained from the crossing of selected inbred parental units capable of producing first generation (F]) hybrid seeds, or from commercial breeders.

The production and selection of inbreeding F lines, which result in the crossing of first order F hybrids, is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of F, hybrids, and thus to market commercially excellent corn.

Due to the significant role of inbred lineages in the production of commercial F, maize hybrids, special emphasis has been placed on the breeding of inbred lineages in the field of maize cultivation and hybridization. Thus, it has become necessary to breed new inbred lines that result in first generation hybrid maize with higher yields, higher crop stability and improved resistance during hybridization. Another requirement is the phenotypic homogeneity of first-generation hybrids, which facilitates mechanical harvesting and reduces manual labor.

There are concerted efforts to develop inbred lines for the production of commercially available high-performance first generation maize hybrids.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line DE 0716 which, when crossed with another inbred corn as a female or male parent unit, produces a first generation (F,) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, the inbred maize line DE 0716 is used, which has high yields, excellent stems and excellent green yielding properties.

The inbred line DE 0716 of the present invention, when used as a parent line, can be crossed with other maize lines, preferably inbred lines, to form first generation (Fj) maize hybrids. Although the inbred line DE 0716 is generally used as a female during crossing, it can also be used as a male.

DE 0716 is an inbred maize line of the yellow horse type and its development is described below:

The origin of the inbred line DE 0716 can be represented by the formula (4744xMO17) MO17HT crossing. The 4744 inbred line is owned by Dekalb Pfizer Genetics. MO17 is a well-known inbred line. (4744xMO17) and MO17HT were crossed in the summer of 1978 in Thomasboro, Illinois, USA. The following breeding process is illustrated in the following table:

Year Horticulture Rows number Place generations rationality Bepor- tion 1979 Disease 1292 Thomasboro, IL s ₀ you 1980 Winter 4262 Homestead, FL And, you 1980 TC Lines 479 Thomasboro, IL S ₂ you 1981 Inbred Inc. 106 Thomasboro, IL S ₃ you

The line received was 4971 A followed by 0716. In the process, selection was made on the basis of general characteristics such as good inbreeding performance, good health and excellent endurance. The 4917A line was maintained by continuous self-pollination and by discarding different plants. The line

The characteristic phenotypic characteristics of HU 206 018 Β are inherited from generation to generation.

The uniform phenotype of line 4917A has been maintained throughout maintenance, development and cultivation.

The inbred line DE 0716 is the property of Dekalb Pfizer Genetics and was not accessible to users.

The invention relates to cells of plants of the inbred line DE 0716 which can be cultured in a suitable culture and then isolated and regenerated to produce plants of the inbred line. Details of the generation process are described in C.E. Green and C.A. Rhoes, Plant Regeneration in Tissue Culture of Maize, Maize Foor Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 0716 is a yellow horsehair type maize which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, 12 November 1980):

Letter, position (in the central third of the plant): SO coat of arms, flowering start: late coat of arms, anthocyanin coloration of anthers: none or very weak Tube, mustache appearance: SO Tube, anthocyanin coloration of mustache: weak coat of arms, anthocyanin coloration of chaff: SO coat of arms, anthocyanin ring at the base of the chaff: there is Shit, anthocyanin coloration of nodules: none or very weak Shit, anthocyanin coloration of nodules (center of plant): weak Letter, anthocyanin (in the center of the plant): weak Letter, hairs on the edge of the coat: weak coat of arms, crankshaft and side angles (in the lower third of the tassel): small coat of arms, position of the side branches (in the lower third of the coat of arms): SO coat of arms, number of primary side branches: few coat of arms, crankshaft length above the lowest sidewall: medium Plant, height (including title): medium Tube, position of the tube in relation to the total height of the plant: medium

Tube, handle length: medium

Tube, length: long

Tube, diameter (center of tube): medium

Tube Shape: Slightly conical

Pipe, number of cores: few

Tube, type of cores (middle third of tube): horse-tooth

Tube color, yellow color

Tube, core backside color: orange

Tube, anthocyanin coloration of the chaff: yes

Tube, anthocyanin coloration of the chaff: medium

The inbred maize line DE 0716 is derived from 15 (4744xMO 17) MO 17 triple crosses.

The inbred maize line DE 0716 is a yellowish equine inbred maize line. The closest known corn line is MO17. The

The MO17HT inbred line was developed from the publicly available MO17 inbred line by cross-linking the Ht gene conferring resistance to Helminthosporium turcicum. The inbred vo25 of DE 0716 is statistically significantly different from the known inbred line M017HT in that the anthocyanin coloration of the mustache is pink and yellowish-green, the angle between the crest of the crest and the crown is slight, 4 cm and 239.6 cm respectively), tube formation on the plant from the ground level: 95 cm and 106 cm respectively, the color of the apex is yellowish or yellow-orange, the anthocyanin coloration of the tube and the chaff is red or pink.

The above characteristics of the inbred line DE 0716 relative to the known inbred line B37Ht are summarized in Table 1:

Table 1

specific DE 0716 MO17HT anthocyanin coloration of mustache pink yellowish green angle between crest and crankshaft small medium plant height (cm) 214 240 tube height (cm) 95 106 color of the apex yellowish yellow-Na rancssárga anthocyanin staining intensity of tube, chaff red pink

An isozyme analysis of the inbred lines DE 0716 and MO17 shows that there are five genetic divergences:

AMD1 = 4 instead of 5,

AMP3 = 4 instead of 3,

ESTI = 4.5 instead of 4,

HU 206 018 Β

GDH = 1 instead of 2,

IDH2 = 6 instead of 4 and

MDH3 = 18 instead of 16.

The isozyme genotype of the inbred line DE 0716 compared to the known inbred line MO17 is shown in Table 2:

Table 2

Place Allelomorphic presence 0716 MO17 ACPH 2 2 ADH1 4 4 AMD1 4 5 AMP3 4 3 Category 3 9 9 Enplo 6 6 EVENING 4.5 4 GDH 1 2 GOTO 4 4 GOTM 4 4 GOTO 4 4 GLU B 6 6 HEX1 4 4 HEX2 2 2 Idh1 4 4 IDH2 6 4 MDH1 6 6 MDH2 6 6 Mdh3 18 16 MDH4 12 12 Mdh5 12 12 ME1 1 - PGM1 9 9 PGM2 4 8 PGD1 3.8 3.8 PGD2 5 5 SAD 4 4 ΤΡΙ1 4 - TPI2 4 - TPI3 4 - TPI4 4 -

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Com and Sorghum Industry Research Conference, 1980.

The inbred line DE 0716 is preferably used as a parent line for the production of hybrid maize, in particular first generation (Fj) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, two inbred lines, one of which is an inbred line DE 0716, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the three-leaf state and regularly treated with fertilizers and / or other agrochemicals, such as fertilizers, for proper development.

During flowering, when the inbred line DE 0716 is used as a male parent unit, the coat of arms of the other parent unit is removed from all plants. This removal can be done manually or mechanically.

Female parental units can be sterilized chemically and simultaneously incorporate a cytoplasmic male sterile property.

The lines are further cultivated, while the species normally cross-pollinated by the wind. As a result of the neutering of the female lineage, all pollen from the male lineage is suitable for pollination since all the plants of the inbred lineage, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties (isolation distance). The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from female parental units are harvested to harvest the seeds of the new Fj hybrid maize. If necessary, seeds from the male vole can also be harvested and used, but these seeds are not suitable as hybrid seeds, only for feeding purposes.

The resulting new F] hybrid seeds can be planted next season, when high yielding F] hybrid maize plants will develop.

The inbred line DE 0716 is suitable for the production of novel F [maize] plants, which exhibit advantageous properties, for example, the inbred line 0716 is used as the male parent line and the DE 0712 inbred line (Dekalb Pfizer Genetics) is used as the female parent line. The first generation (F,) hybrid received the EXP 85 signal.

The characteristics of the first generation (F,) EXP 858 hybrid were compared with those of the commercially available commercial DK 547 (Dekalb Pfizer Genetics) and Pioneer 3475 or Volga. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

HU 206 018 Β

Table 3 EXP858andDK 547

Hybrid Fruit (bush) * Moisture content (%) Flowering time (single) Plant height (cm) tube Height (Cm) Knee deflection (%) Heeling root (%) EXP858 126.5 19.8 1442 194 86 3.3 2.5 DK 547 120.6 X 17.8 XX 1397 XX . 191 91 + 4.5 X 3.1

Number of test sites: 47

EXP858 and PIONEER 3475

Hybrid Fruit (bush) * Moisture content (%) Flowering time (single) Plant height (cm) tube Height (Cm) Knee deflection (%) Heeling root (%) EXP858 125.1 20.0 1442 194 86 3.6 2.5 Pioneer 120.9 18.4 1397 193 85 2.7 3.9 3475 + XX XX XX + X

Number of test sites: 51 * 1 bush-36.35 liters.

x is statistically significant at the 5% level

From the foregoing, the inbred line DE 0716 provides a preferred breeding strain when used as a male or female parent line. With its use, first generation hybrids with special and advantageous properties are adjustable. 30

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention. 35

Claims (1)

Patent Claim Point DE 0716 is a male inbred line from 40 crosses of (4744xMO17) xMO17 and xx is statistically significant at 1% + statistically significant at 10%. late flowering, anthocyanin coloration of anthers absent or very poor, anthocyanin ring at the base of the pod, anthocyanin coloration of nodules absent or very weak, anthocyanin coloration of nodules poor, anthocyanin coloration of leaf envelope weak, the number of primary side branches is small in the crest, the length of the crankshaft above the lowest side branch is medium, the length of the tube is medium, the tube is long and slightly conical, the diameter is medium, the number of cores is small. the anthocyanin color of the mustache is low, the angle between the crest and the side of the tassel is small, the height of the plant is 210-218 cm, preferably 214 cm, the tube fitting height is 90-100 cm, preferably 95 cm, the anthocyanin color of the apex medium.