HU202045B - Inbred maize line marked by de 8015 - Google Patents

Abstract

FIELD OF THE INVENTION: Inbred maize line DE 8015, produced by hybridization IBIx7309B, where the appearance of the mustache is medium, the anthocyanin ring at the base of the chaff is absent, the anthocyanin discoloration of the nodes and nodes is absent, the anthocyanin discoloration of the leaf envelope is absent, the leaf is poorly hairy, the leaf is weakly hairy the headline is the angle of the crankshaft and the side branches, the sides of the side bend backwards, the length of the crankshaft is short, the tube is of medium length, the diameter is medium, its shape is slightly conical, the number of core lines is a few, the type of seeds is toothed, the color of the core tip is yellow, the color of the core is yellow, the core is yellow, the back of the core is yellow its color is orange, the flake has an intense anthocyanin coloration. A characteristic feature of the invention is that the number of days until the appearance of the mustache is 79, the height of the plant is 217.1 cm, and the height of the tube is 100.9 cm, the number of branches in the coat is 10.5, the color of the ports is red, the color of the mustache is yellowish green. , the diameter of the tube is 39.6 cm, and the color of the cob is brown. in Scope of the description: 8 pages, Figure 1

Description

The present invention relates to an inbred maize line DE 8015.

Zea mays, or corn, is a major crop in many countries around the world, especially in the United States. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of hybridization, varieties with significantly improved yields, stronger stems and roots and more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize cannot be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (F1) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of F1 hybrid plants produces second-generation (F2) hybrids. F2 hybrid plants and seeds have less favorable properties than F1 hybrid, thus yielding lower yields and expressing undesirable genetic characteristics. Due to the reduced characteristics, the seeds of the less favorable second generation F2 hybrids F1 are not harvested by growers. On the contrary, growers expect new hybrid seeds obtained each year from the crossing of selected inbred parental units capable of producing first generation (Fi) hybrid seeds.

The production and selection of inbred lines resulting in crossing F1 hybrid hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of F1 hybrids and thus to be commercialized.

Due to the important role of inbred lineages in the production of commercial F1 maize hybrids, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that yield higher yields during hybridization to produce today's first generation hybrid maize with greater stability and better resistance. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

Accordingly, it is an object of the present invention to provide a new inbred corn line DE 8015.

It is therefore an object of the present invention to provide a new inbred line DE 8015 which, when crossed with another inbred maize as a female or male parent, produces a first generation (Fi) hybrid with advantageous characteristics.

The insemination of other maize varieties with the pollen of the inbred line DE 8015 of the present invention results in a high quality first generation (F1) maize hybrid.

The inbred line DE 8015 of the present invention can be used to produce a maize hybrid, which results in a first generation (Fi) hybrid maize with advantageous properties such as high yield, good stability, good insect and disease resistance.

Thus, the present invention utilizes the DE 8015 inbred line, which has high yield, excellent stalk and excellent greening properties.

The hybrid maize of the present invention is produced by crossing the first maize line with a second maize line, wherein the first maize line or second maize line is an inbred maize line DE 8015 and the other line is a different inbred maize line or cross.

In the production of a new hybrid maize variety, we use:

(a) planting seeds of inbred line DE 8015 and seeds of other maize line in the proximity of pollination;

b) cultivating the plants resulting from planting until flowering;

c) inoculating plants from one of the corn lines;

d) cross-pollinating between plants from the inbred line DE 8015 and the other maize line; and

e) harvesting seeds of plants of the other maize line, wherein the other maize line may be a hybrid maize cross or an inbred maize line, which is preferably not the same as DE 8015.

The inbred line DE 8015 of the present invention, when used as a parent line, can be crossed with other maize lines, preferably inbred lines, to form first generation (F1) maize hybrids. Although the inbred line DE 8015 is generally used as a female during crossing, it can also be used as a male.

HU 202045 Β

DE 8015 inbred corn line is a yellow horse-bred inbred line and its development is described below:

The DE 8015 line was produced by standard hybridization cross between the IBI inbred line and the 7309B inbred line. The IBI line is self-pollinated from the Pioneer 3901 hybrid and maintained by Dekalb Pfizer Genetics. The inbreed line 7309B is a self breeding of the Dekalb Pfizer Genetics from the OH43 family. Specifically, by crossing the IBI and 7309B lines, we generated a Fi generation in Madison, Wisconsin. This Fi generation was crossbred with the IBI inbred line in Maui, Hawaii. The resulting separate family was raised in Madison, Wisconsin. Of the approximately 2000 plants, 200 were self-pollinated and only 35 were harvested. Of these 35, we raised the S2 family in Maui, Hawaii. 12 rows were self-pollinated. We harvested these 12 rows and raised a total of 31 S3 rows in Madison, Wisconsin.

Three rows were selected. From these, two different inbred experimental lines B14 were generated by self pollination and crossing. One or more tubes were planted from these rows. The plants were self-pollinated and S5 rows were planted in Madison, Wisconsin. An inbred line DE 8015 was isolated based on a crossover experiment. This is called IB02. For inbreeding, self-pollinated seeds of line S5 were accumulated. This is known as DE 8015. The pure origin of line DE 8015 is maintained by self-pollination and by the accumulation of cores from selected tubes of all generations.

The line is homozygous for the genes that regulate uniformity and the main traits observed.

Line DE 8015 is maintained as a breeding seed in Madison, Wisconsin, where it forms a constant and uniform inbred line. It does not show any volatility or instability even after five self-pollinated generations.

The DE 8015 inbred maize line is the property of Dekalb Pfizer Genetics and was not accessible to users.

The seeds of the inbred line developed during the described inbreds were deposited under U.S. Department of Agriculture, Agricultural Marketing Service, Livestock, Poultry, Grain and Seed Division, Plant Variety Protection Office, National Agricultural Library Building, Beltsville, Maryland 20705.

The cells of the plants of the inbred line DE 8015 according to the invention can be cultured in an appropriate culture, then separated and regenerated to produce the plants of the inbred line.

For details on the generation process, see C.E. Green and C.A. Rhodes, Plant Regeneration in Tissue Culture of Maize; Maize for Biological Research, W. F. Sheridan Publication, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 8015 is a yellow horse-toothed corn which is preferably adapted for cultivation in the northern corn zone of the United States (especially in the state of Iowa).

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogenity and Stability for Maize, 12/11/1980):

Coat of arms, beginning of flowering: medium

Coat of arms, anthocyanin coloration of anthers: strong

Tube, hair appearance: medium

Tube, hair anthocyanin coloration: weak

Coat of arms, anthocyanin ring at the base of the husk: yes Stem, anthocyanin lumps: no or very weak

Stems, lumps anthocyanin discoloration (center of plant): none or very weak

Anthocyanin color of leaf, envelope (in the center of the plant): none or very weak

Leaf, hairs on outer edge of coat: weak Coat of arms, crankshaft and side branches (lower third of coat of arms): medium

Coat of arms, position of lateral branches (lower third of coat of arms): Foldable

Coat of arms, number of primary side branches: many

Coat of arms. crankshaft length above the lowest sidewall: short

Plant height (incl. Coat of arms): long Pipe, tube fitting relative to plant height: low

Tube, length: medium

Tube, diameter (center of tube): medium

Tube Shape: Slightly conical

Pipe, number of lines: a few

Tube, type of cores (middle third of tube): horse-tooth

Tube color, yellow color

Tube, core backside color: orange yellow

Tube, anthocyanin coloration of the chaff: yes

Tube, intensity of anthocyanin staining of the chaff: strong.

The inbred maize line DE 8015 is a yellow inbred inbred line. The closest known maize line is A 619Ht. The A619Ht inbred line was developed from the publicly available inbred line A619 by introducing the Htl gene conferring resistance to Helminthosporium turcicum. The inbred line DE 8015 is statistically significantly different from the known inbred line A619Ht at plant height (217 cm and 193 cm), tube height (101 cm and 62 cm), anthers (red and yellowish green) and hair color (red or yellowish green). In addition, there is a distinctive feature in the diameter of the tube (39.6 mm and 46.3 mm) and in the color of the cube (brown and white). Line 8015 is also significantly different from A619Ht in terms of maturation. DE 8015 blooms 5 days earlier than A619H (see Table 1).

The above characteristics of the inbred line DE 8015 relative to the known inbred line A619Ht are summarized in Table 1.

HU 202045 Β

Table 1

Plant and tube DE 8015 A619Ht Measurement characteristic

Mm Ho: MuiMü2 Hi: Mui · *

1. Up to 50% hair appearance

number of days elapsed 2nd inc. height (cm) 79 217.1 84 193.4 * * Tube height 3 (cm) 100.9 62.1 * 4. number of branches in coat of arms 10.5 9.2 5. color of the ports red yellow / green * Weight of tube 6 (g) 87.6 91.8 7. hair color yellow / green yellow / green Diameter of tube 8 (cm) 39.6 46.3 « 9th color of the cob brown f eher

• with a significant probability of 0.1

Isozyme analysis of the inborn lines IB02 and A619Ht show genetic differences at three different sites:

Acpl = 2.4 instead of 4,

Mdh2-6 instead of 3 and Mdh3 18 instead of 16.

The isozyme genotype of the inborn line IB02 is compared with the known inbred line A619Ht in Table 2:

Table 2

Location Allelomorph presence

IB02 A619Ht

Number of plants tested 12 6 ACPL 2.4 4 adhl 4 4 Cat3 9 9 Enplo 6 6 Got3 4 4 Got2 4 4 Gotl 4 4 Idhl 4 4 Idh2 6 6 Mdhl 6 * 6 Mdh2 6 3 Mdh3 18 16 Mdh4 12 12 Mdh5 12 12 pgml 9 9 Pgm2 4 4 Pgdl 3.8 3.8 Pgd2 5 5 Phl 4 4

* the allelomorph is assumed to be 6, but zero cannot be excluded.

Technique for Isozyme Genotyping Goodman, Μ. N. and Stuber, C. W .; Genetic identification of lines and crosses using isoenzyme electrophoresis; Proceedings of the Thirty-Fifth Annual

Com and Sorghum Industry Research Conference, 1980.

The inbred line DE 8015 is preferably used as a parent line for the production of hybrid maize, in particular first generation (F1) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, two inbred lines, one of which is an inbred line DE 8015, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DE 8015 is used as a male parent unit, the coat of arms of the other parent unit is removed from all plants. This removal can be done manually or mechanically. Female parental units can also be chemically sterilized by a cytoplasmic male sterile property. When line DE 8015 is used as a female parental unit, the coat of arms is removed while pollen develops normally on the other parental unit. In one case, the line is allowed to grow further, so that the wind causes cross-pollination.

As a result of the neutering of the female lineage, all pollen from the male lineage is suitable for pollination since all the plants of the inbred lineage, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from the female parent units are harvested to harvest the seeds of the new F1 hybrid maize. Seeds from the male parent unit can also be harvested and used as needed, but these seeds are not suitable as hybrid seeds.

The resulting new Fi hybrid seeds can be planted next season, when high-yield Fi hybrid maize plants will develop.

The inbred line DE 8015 is suitable for the production of new F1 maize plants, which exhibit advantageous properties, for example, the inbred line DE 8015 is used as a female inbred line and the commercially inbred line DE 0660 is used as a male inbred line.

The characteristics of the first generation (Fi) DK397 hybrid were compared with those of the commercially available DK399 (Dekalb-Pfizer Genetics) variant. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

HU 202045 Β

Table 3

Hybrid DK397 DK399 Product (bus) 123.4 110.7 "* Moisture content of seed (%) 21.7 22.0 * Plant height (inch) 82.1 74.2 »* Pipe height (inch) 37.1 36.9 Stem not lying (%) 103.9 98.6 ♦ ♦ Root not lying (%) 101.1 99.9 **

* -5% Statistical Significant (1 inch-36.35 liters) ** - 1% Statistical Significant (1 inch-2.54 cm) Placement Number: 209.

It can be seen from the foregoing that the inbred line DE 8015 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous properties.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention.

Claims (1)

Patent claim DE 8015, inbred corn line, produced by hybridization with IBIx7309B, which has a medium mustache appearance, lack of anthocyanin at the base of the chaff, anthocyanin discoloration of nodules and nodules, headlines are medium crankshaft and side branches angular, side branches inclined, crankshaft short, tube medium long, medium diameter, slightly conical, number of kernels a few, kernel type is equine, kernel color is yellow orange, with an intense anthocyanin coloration on the chaff, characterized by 79 days to mustache appearance, plant height 217.1 cm, tube fitting height 100.9 cm, number of branches at coat of arms 10.5, port color red, mustache color yellowish green, cs he is 39.6 cm in diameter and the color of the cob is brown.