HU202355B - Male inbred maize line of sign 8016 - Google Patents

Abstract

FIELD OF THE INVENTION The male inbred maize line DE8016, produced by the Pioneer 3901xJ61 cross, with a slight return to the leaf, the beginning of flowering is medium, the anthocyanin discoloration of the pores is strong, the appearance of the mustache is moderate, the anthocyanin coloration is strong, and the anthocyanin is anthocyaninic. the anthocyanin discolouration of the nodes and nodes is absent, the anthocyanin discoloration of the chaff is strong, the anthocyanin discoloration of the leaf envelope is medium, the coat is heavily hairy, the coat of arms of the crankshaft and the lateral branches is large, the side branches recline, the number of the lateral branches bends back, the number is large, the cranks are drooping, the crankshaft is long the diameter of the tube is medium, its shape is slightly conical, the number of core lines is medium, the type of seeds is toothed, the color of the nucleus is yellow, the back color of the core is orange, and the anthocyanin discoloration of the chaff is strong. A characteristic feature of the invention is that the plant has a height of 117.33 cm, a hose height of 68.6 cm, a tube length of 14.32 cm, and a length of the stem of 17.55 cm. (Figure 1/1) Scope of the description: page 8, Figure 1 CQ LO lf) CO CM o CM -1-

Description

The present invention relates to a male inbred line DE8016.

Zea mays, or corn, is a major crop in many countries around the world, especially in the United States. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (F1) hybrids, for example by hybridizing two inbred lines.

By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of F1 hybrid plants produces second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the F1 hybrid, thus yielding lower yields and expressing undesirable genetic characteristics. Due to the reduced characteristics, the seeds of the less favorable second generation F ₂ hybrids are not harvested by the growers. On the contrary, growers are expecting new hybrid seeds obtained each year by crossing selected inbred parental units capable of producing first generation (Fi) hybrid seeds.

The production and selection of inbred lines resulting in crossing F1 hybrid hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of F1 hybrids and thus to be commercialized.

Due to the important role of inbred lineages in the production of commercial F1 maize hybrids, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines which, through hybridization, result in today's first generation hybrid maize with greater stability and greater resistance. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

Accordingly, it is an object of the present invention to provide a new inbred maize line DE8016.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line, DE8016, which, when crossed with another inbred corn as a female or male parent, produces a first generation (F1) hybrid with advantageous characteristics.

By using pollen of the inbred line DE8016 of the present invention, fertilizing other maize varieties results in a high quality first generation (F1) maize hybrid.

The inbred line DE8016 of the present invention can be used to produce a maize hybrid which yields a first generation (Fi) hybrid maize with advantageous properties such as high yield, good stability, insect and disease resistance.

Thus, the present invention utilizes the inbred corn line DE8016 which has high yield, excellent stalk and excellent greening properties.

The hybrid maize of the present invention is produced by crossing the first maize line with a second maize line, wherein the first maize line or the second maize line is an inbred corn line DE8016 and the other line is a different inbred corn line.

In the production of a new hybrid maize variety, we use:

(a) planting seeds of inbred line DE8016 and seeds of other maize line in the proximity of pollination;

b) cultivating the plants resulting from planting until flowering;

c) germinating plants from one of the corn lines;

d) cross-pollinating between plants from the inbred line DE8016 and the other maize line; and

e) harvesting seeds of plants of the other maize line, wherein the other maize line may be a hybrid maize cross or an inbred maize line, which is preferably not the same as DE8016.

The inbred line DE8016 of the present invention, when used as a parent line, can be crossed with other maize lines, preferably inbred lines, to form first generation (F1) maize hybrids. Although the inbred line DE8016 is generally used as a male in crossing, it can also be used as a female.

HU 202355 Β

DE8016 inbred maize line is a yellow horse-bred inbred line and its development is described below:

The DE8016 inbreed line comes from a cross between Pioneer 3901 and Jolla. The seeds of Pioneer 3901 5 and the Well were planted and cross pollinated in Maui, Hawaii. The following year, the BC1 population was planted in three rows in Olivia, Minnesota, and 70 tubes were collected after cross-pollination. The 70 tubes were then grown in 10 rows in Kihei. 8016 is derived from tubes collected from one of the rows. Only one tube is selected from this row. Subsequently, the tube-derived S3 plants were grown on Olive and three self-pollinated tubes were harvested. The following year, 15 were planted on each row in Olivia and three self-pollinated tubes were collected from the most preferred row.

These three tubes were planted per row in Olivia the following season, and one tube was collected. This tube was self-pollinated in Hawaii. The seeds of the self-pollinated tubes were collected and labeled with IBI6. The inbred line is maintained by self-pollination and accumulation. This inbred line has been designated DE8016.

The seeds of the inbred line developed during the described inbreds were deposited with the French Ministry of Agriculture at the General Secretariat of the Committee for the Protection of New Varieties (17, Avenue de Tousville, 75007 Paris, France).

Cells of plants of the inbred line 30 of the invention DE8016 may be cultured in appropriate culture, then separated and regenerated to produce plants of the inbred line. For details on the generation process, see C.E. Green and C.A. Rhodes, Plant Regeneration In Tissue 35 Culture of Maize; Bread For Biological Research,

W. F. Sheridan Publisher Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 8016 is a yellow horse-toothed corn, which is preferably adapted for cultivation in the northern corn zone of the United States.

This inbred line has the following characteristics:

Leaf, standing (in the central third of the plant): slightly sloping

Coat of arms, beginning of flowering: medium

Coat of arms, anthocyanin coloration of anthers: strong

Tube, hair appearance: medium

Tube, hair anthocyanin coloration: strong

Coat of arms, anthocyanin ring at the base of the chaff: yes

Stem, lumps anthocyanin coloration: none or very weak

Stems, lumps anthocyanin discoloration (center of plant): none or very weak

Coat of arms, chaff anthocyanin: strong

Anthocyanin color of leaf, envelope (middle of plant): medium

Leaf, hairs on the brim: strong

Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): Large

Coat of arms, position of lateral branches (lower third of coat of arms): Foldable

Coat of arms, number of primary side branches: many

Coat of arms, length of crankshaft above lowermost side: medium

Plant height (including tassel): medium

Pipe, location of the tube fitting relative to the total height of the plant: low

Pipe length: long

Tube, diameter (center of tube): medium

Tube Shape: Slightly conical

Pipe, number of cores: medium

Tube, type of cores (middle third of tube): horse-tooth

Tube color, yellow color

Tube, core backside color: orange

Tube, anthocyanin coloration of the chaff: yes

Tube, intensity of anthocyanin staining of the chaff: strong

The inbred corn line DE8016 is a yellow horse-bred inbred line derived from the crossing of a single crossing with an inbred line (Pioneer 3901xJ61). The nearest known maize line is the A632H. The 8016 40 inbred line is statistically significantly different from the known A632Ht inbred line at plant height (117.33 cm and 144.33 cm), tube height (68.6 cm and 81.77 cm), and tube length (14). , 33 cm and 17.94 cm, respectively) and the length of the peduncle (17.55 cm and 14.74 cm, respectively).

The above characteristics of the DE8016 inbred line relative to the known A632Ht inbred line are summarized in Table 1:

Table 1

specific DE8016 A632Ht Hypothesis of measurement Ho: Mu, = Mu ₂ Ημ Mui ^ Muí Plant height 1 (cm) 117.33 144.33 * Tube height 2 (cm) 68.6 81.77 * Length of tube 3 (cm) 14.32 17.94 * Length of stalk 4 (cm) 17.55 14.74 *

* significant level: 0.1%

-3HU 202355 Β

An isozyme analysis of the inbred lines DE8016 and A632Ht shows that there are four different genetic differences:

B-Glu-6 instead of 7,

MDHB = 3.5 instead of 6,

PHI = 4 instead of 5.

The isozyme genotype of the inbred line DE8016 is compared to the known A632Ht inbred line in Table 2.

Table 2

Place Allelomorpf presence DE8016 A632Ht Plants tested number 6 6 ACPH 4 4 ADH 4 4 Cat 9 9 INTACT 6 6 GOT U 4 4 GOT M 2 4 GOT L 4 4 GLU B 6 7 IDH A 4 4 IDH B 6 6 MDH A 6 * 6 * MDH B 3.5 6 MDH C 16 16 MDH D 12 12 MDH E 12 12 PGM A 9 9 PGM B 4 4 PHI 4 5

* = allelomorpf is assumed to be 6, but zero cannot be excluded.

Technique for Isozyme Genotyping Goodman, Μ. N. and Stuber, C. W .: Genetic Identification of Lines and Crosses Using isoenzyme electrophoresis; Proceedings of the Thirty-Fifth Annual Com and Sorghum Industry Research Conference, 1980.

The inbred line DE8016 is preferably used as a parent line for the production of hybrid maize, in particular 45 first generation (FO hybrid maize. In use, this inbred line is used in combination with another inbred line and hybridizes the two lines with one of the conventional techniques. the inbred line 50 8016 is planted in close proximity to each other for pollination, this can be done by planting the lineages in alternating rows, blocks or by any other means The plants of the two lines are cultivated until flowering. and are regularly thinned in the triple leaf condition and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DE8016 is used as a male parent unit, the coat of arms of the other parent unit is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property. The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female lineage, all pollen from the male lineage is suitable for pollination since all the plants of the inbred lineage, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental individuals are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from female parent units are harvested to harvest the seeds of the new F1 hybrid maize. If necessary, seeds from the male parent can also be harvested and used, but these seeds are not suitable as hybrid seeds.

The resulting new Fi hybrid seeds can be planted next season, when high-yield Fi hybrid maize plants will develop.

The inbred line DE8016 is suitable for the production of new F1 maize plants, which exhibit advantageous properties, for example, the inbred line DE8016 is used as a female inbred line and the commercially inbred line DE6150 is used as a male inbred line. The first generation (Fi) hybrid received the SK25235 mark.

The characteristics of the first generation hybrid (FJ SK25235) were compared with those of the commercially available Marco variant (Dekalb-Pfizer Genetics). Both variants show similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3

Hybrid SNK25235 Marco 1. Yield (q / ha) '99.4 96.5 ** 2. Seed moisture content (t%) 29.7 31.1 ** 3. Stem not lying (%) 8.0 12.9 * 4. The root does not lie (%) 0.7 2.8 *

* -5% Statistical Significant ** = 1% Statistical Significant Placement Number: 12

HU 202355 Β

It can be seen from the foregoing that the inbred line DE8016 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous properties.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention.

Claims (1)

Patent Claim Point Male inbred line DE8016, produced by crossing Pioneer 3901xJ61 with slightly sloping leaf position, medium onset of flowering, strong anthocyanin coloration of anthers, medium appearance of anthocyanin, strong anthocyanin coloration, lack of anthocyanin coloration of nodules and nodules, strong anthocyanin coloration of chaff, medium anthocyanin color of envelope, very hairy, crest of crest and lateral branches, stance of lateral branches many, middle of crankshaft, its shape is slightly conical, the number of seeds is medium, the type of horse is toothed, the color of the apex is yellow, the backside of the seed is orange and the anthocyanin coloration of the pellet is strong, characterized by plant height 117.33 cm cm, a The tube has a length of 14.32 cm and the stem has a length of 17.55 cm.