HU209660B - De 6169 variety of male inbred corn-line (zea mays l.) - Google Patents

Abstract

Field of Invention The invention relates to an inbred maize line DE 6169 derived from a single cross-linked commercial hybrid, where the start of flowering is medium, the anthocyanin discoloration of the pores is absent, the anthocyanin discoloration of the mustache is strong, and the anthocyanin discoloration of the fluff is lacking. the anthocyanin coloration of the nodes is missing, the anthocyanin discolouration of the leaf envelope is poor, the angle between the crankshaft and the lateral sides of the coat of arms is small, the main axis of the coat of arms is short over the lower lateral branch and medium length over the top side of the coat; its handle is short, its diameter is small, its shape is slightly conical, the number of seed rows is small, the core type is horse-toothed, the tip of the core is orange, its orange side is orange and the anthocyanin discoloration of the chaff is missing on the tube, the height of the plant is small, al the angle of the eagle to the stem is medium, the color after the pollination is bright green, the fluff of the leaf envelope is strong, there are many waves on the edge of the leaf, the number of the primary side of the coat of arms is a few, the color of the ports is greenish yellow, the closed anthocyanic ring is missing at the crest of the crest, tube is medium length and the shell is open. EN 209 660 B Scope of the description: 8 pages (including 2 sheets)

Description

The present invention relates to a male inbred line of the breed DE 6169.

Zea mays L., or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. The F! self-pollination or cross-pollination of hybrid plants produces second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the Fj hybrid, thus yielding lower and undesirable genetic characteristics. Due to the reduced characteristics, F! Resulting in a less favorable second generation F ₂ hybrid. The seeds of hybrids are not harvested by growers. Each year, growers expect new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (Fi) hybrid seeds.

The production and selection of inbred lines resulting in crossing F1 hybrids is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of F, hybrids and thus to be commercialized.

Due to the significant role of inbred lineages in the production of commercial maize hybrids, the development of inbred lineages has received particular emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line of the variety DE 6169.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line of the breed DE 6169, characterized in that when crossed with other inbred maize as male or female parent units, a first generation (F0) hybrid is obtained with advantageous characteristics.

Thus, in accordance with the present invention, an inbred corn line DE 6169 is used which has high yield, excellent stalk and excellent greening properties.

The inbred maize line DE 6169 of the present invention can be used to produce hybrid maize by crossing a first maize line with a second maize line, wherein a first maize line or a second corn line is used to produce an inbred maize line or a second maize line.

The inbred line DE 6169 of the present invention, when used as a lineage, can be crossed with other maize lines, preferably inbred maize lines, to form first generation (Fj) maize hybrids. Although the inbred line DE 6169 is generally used as a male in crossing, it can also be used as a female.

Inbred maize line DE 6169 is a yellow horse-bred inbred line and its development is described below:

The line was developed in Druelle (Aveyron, France) corn horticulture. The altitude of the horticulture is 580 m. This horticulture is part of the G. I. E. Sockalb corn breeding program.

1979, S _o *

In a commercial hybrid population of 400 plants, 80 self-pollination was performed. 15 plants and tubes were selected for harvest. Selection was based on flowering time, stem quality and tube size. The above selection criteria were applied throughout the breeding process.

* abbreviation for S = inbred name. The index number is inbreeding! degrees.

HU 209,660 Β

1980, Sj

The seeds of the 15 tubes were planted in separate rows per tube, with 40 plants per row at a density of 100,000 plants / ha. The 10 earliest plants were pollinated per row. For harvesting, a total of 10 tubes were selected from 6 rows.

1981, S ₂

The procedure described for Generation Sj was repeated, with 10 tubes per row planted. At harvest, 7 tubes were set aside and 12 tubes were used.

1982, S ₃

The procedure described for Generation S ₂ was repeated, where 12 tubes per row were planted for pollination. At the same time, a portion of the selected tube 12 was transplanted into an isolated crossing block where it was crossed with a test horse plant. According to the gardeners' observations, 4 rows were discarded and 9 of the 8 rows were harvested.

1983, S ₄

The harvested S ₃ seeds were planted and pollinated. The experimental crosses were tested at two sites in Bassin Parisien (Provins, Chateaudun). At harvest, one experimental cross was maintained, with a selection index of 107, which was compared to LG11. The S ₄ generation of the above S ₃ experimental cross was pollinated and harvested. The rest were dropped.

1984, S ₅

The new inbred line received the SK117 mark, which is identical to the line bred in DE 6169. The line is also known as HTVA3-2.

S ₅ generations per line were planted and pollinated four tubes. In addition, hybrids were formed in several crossing blocks.

1985, S ₆

S ₅ generations of progeny grown horticultural seeds to propagate. The experimental crosses were examined at 3 sites.

1986

Experimental crossing DE 8016XDE 6169 entered the pre-registration phase. The studies were conducted in 6 locations throughout northern France.

The inbred line DE 6169 has been shown to be distinct, uniform and stable in respect of the characteristics tested.

The inbred line DE 6169 is owned by Dekalb Plant Genetics and was not accessible to users.

The invention relates to cells of the inbred line of DE 6169, which can be cultured in a suitable culture, then separated and regenerated to produce the inbred line. Details of the generation process are described in C.E. Green and C.A. Rhoes, Plant Regeneration in Tissue Culture of Maize, Maize Foor Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 6169 is a yellow horse-toothed corn, which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, November 12, 1980):

Coat of arms, beginning of flowering: early

Coat of arms, anthocyanin coloration of anthers: none or very weak

Pipe, hair appearance (the plants

50%): early

Tube, hair anthocyanin coloration: strong

Anthocyanin coloration of coat of arms, chaff: none or very weak

Coat of arms, closed anthocyanin ring at the base of the chaff: none

Anthocyanin coloration of stems, nodules: none or very weak

Anthocyanin coloration of stems, nodules (in the center of the plant): none or very weak

Leaf, envelope fluffy: strong

Anthocyanin color of leaf, envelope (in the center of plant): weak

Leaf, edges: many waves

Leaf, angle to stem: medium

Leaf, hairs on the brim: strong

Leaf, color after pollination: light green

Coat of arms, crankshaft and side angles (in the lower third of the coat of arms): small

Coat of arms, number of primary branches: few

Coat of arms, length of crankshaft above the lowest side branch: short

Coat of arms, length of crankshaft above upper side: medium

Ports, color: greenish yellow

Plant height (including tassel): short

Pipe, tube fitting relative to total plant height: medium Pipe, handle length: short

Tube, casing: open

Tube, length: medium

Tube diameter (center of tube): Thin

Tube Shape: Slightly conical

Pipe, number of lines: a few

Tube, type of cores (middle third of tube): horse-tooth

Tube color, orange color

Tube, core backside color: orange

Tube, anthocyanin coloration of the chaff: none

Intensity of anthocyanin coloration of tube, chaff: very weak

Figures 1 and 2 show the shape of the corn kernels and Figure 3 shows the corn tube with a cob.

The inbred maize line DE 6169 is a yellow horse-toothed inbred line derived from an organic hybrid commercial hybrid. The nearest known maize line is F ₂ . The inbred line DE 6169 is statistically significant3

HU 209 660 Β san differs from the known inbred line F ₂ in germination capacity (2 and 4), plant height (155.2 cm and 144.8 cm, respectively), leaf angle (medium and up), pollination color (light green or medium green), leaf fluff (strong or weak), leaf waviness (many or few), ear length (20.3 cm and 16.4 cm, respectively), tassel primer the number of side branches (4.0 and 9.7, respectively), the color of the anthers (greenish yellow and yellow), the anthocyanin ring at the base of the coat of arms (none), the length of the tube (14.3 cm and 10, 8 cm), along the length of the overhang (1.9 cm and 7.2 cm respectively) and the appearance of the shell (open or close).

The above characteristics of the inbred line DE 6169 relative to the known inbred line F ₂ are summarized in Table 1:

Table 1

specific DE 6169 f ₂ THE B germination capacity (1-5) 2 4 plant height (cm) 155.2 144.8 40.186 12.4 leaf angle medium up color after pollination clear green medium green leaf envelope fluffiness strong weak leafwave ripple many few Length of corn (cm) 20.3 16.4 2,432 3.1 number of primary side branches of tassel 4.0 9.7 1,120 2.1 color of ports greenish yellow yellow closed ring no there is tube length (cm) 14.3 10.8 0.558 1.5 envelope coverage (cm) 1.9 7.2 0.891 1.9 shell appearance open tight

notation: A = square of average deviation

B = standard deviation (significance 0.05)

Isozyme analysis of the inbred lines DE 6169 and F ₂ show that there is at least three genetic differences: Acphl = 3 instead of 2

PgmB = 4 instead of 2.

The isozyme genotype of the inbred line DE 6169 with the known inbred line F _{2 is} shown in Table 2.

Table 2

Place Allelomorphic presence Acphl 3 2 adhl 6 6 Cat3 9 9

Place Allelomorphic presence Got3 4 4 Got2 4 4 Gotl 4 4 Glu B 7 7 ldhA 4 4 IdhB 4 4 MdhA 6+ 6+ MdhB 6 6 MdhC 16 16 MdhD 12 12 MdhE 15 15 Pgm A 9 9 6 PgdA 3.8 3.8 6 PgdB 5 5 Phi 4 4 number of plants tested 6 6

notation: + = presumably 6, but 0 cannot be excluded.

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Com and Sorghum Industries Research Conference, 1980.

The inbred line DE 6169 is preferably used as a parent line for the production of hybrid maize, in particular first generation (Fj) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the inbred line DE 6169, are planted in close proximity to each other for pollination. This can be accomplished by placing the parent lines in alternating rows, blocks or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DE 6169 is used as a parent, the coat of arms of the other parent is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female parent line, only the pollen from the male parent line is suitable for pollination since all the plants of the inbred line, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed.

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HU 209 660 B

During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known in the art (isolation distance).

Subsequently, it is cultivated from both parental lines to maturity, but only tubes from female parental units are harvested to collect the seeds of the new Fj hybrid maize. Seeds from the male parent unit can also be harvested and used as needed, but these seeds are not suitable as hybrid seeds.

The resulting new F1 hybrid seeds can be planted next season, when high yield Fj hybrid maize plants will develop.

The inbred line DE 6169 is suitable for the production of novel maize plants, which exhibit advantageous properties, for example, the inbred line DE 6169 is used as the male parent line and DE 8016 is the female inbred line. The first generation (Fj) hybrid received the DK 205 signal. The characteristics of the DK 205 hybrid were compared with those of the commercially available LG 2080 variant. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3 DK 205 and LG 2080

Hybrid crop (Bushel) * Moisture content (%) stem Drop (%) Roots (%) DK 205 85.4 32.1 6.1 8.6 LG 2080 79.3 32.8 5.0 15.1 XX X XX

number of places: 47 x = statistically significant at 5% xx = statistically significant at 1% + = statistically significant at 10%.

* 1 bush = 36.35 liters.

It can be seen from the foregoing that the inbred line DE 6169 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous characteristics.

Claims (1)

Patent Claim Point DE 6169 inbred maize line, derived from a single-hybrid commercial hybrid with a moderate onset of flowering, lack of anthocyanin coloration of anthers, early onset of mustache, strong anthocyanin coloration of mustache, anthocyanin coloration of chaff, , lack of anthocyanin coloration of knot spacing, weak anthocyanin color of leaf envelope, small angle of crest axis and side branches, crest crest short at low side and mid long side, tube fitting height to medium short, , slightly conic in shape, small number of kernels, horse type, kernel orange, orange backside and lacking anthocyanin coloration of chaff, with low plant height, leaf angle moderate to stem, post-pollination color light green, leaf envelope strong, many waves at the edge of the leaf, some number of primary crest branches, anthery color greenish yellow, closed anthocyanin ring at the base of the coat of arms, tube moderately long and open.