HU209665B - De 8017 variety of female inbred corl-line (zea mays l.) - Google Patents

Abstract

Field of the Invention: Inbred maize line DE 8017, obtained by crossing the hybrid and inbred line P 3901xJ 61, where the start of flowering is medium, the appearance of the mustache is medium, the anthocyanin discoloration of the mustache is moderate, the anthocyanin discolouration of the chaff is absent, closed the anthocyanic ring is missing at the base of the chaff, the anthocyanin discoloration of the nodes is missing, the anthocyanin discoloration of the nodes is missing, the fluffiness of the edge of the sheath is moderate, the number of primary side branches is medium, the length of the crankshaft is medium above the lower branch, the crankshaft is medium at the top branch, the crankshaft is medium at the top branch, the crankshaft is medium at the top branch tube length is medium, the tube is short, its diameter is medium and slightly conical, the number of core lines is medium, the core type is horse-toothed, the tip of the core is yellow, the back is orange and the anthocyanin coloration of the cob is moderate, the height of the plant is medium, the tube is medium, the tube fits well its height is medium, the anthocyanin discoloration of the stem is not very weak, the color of the supporting roots is red, the angle of the leaf is upwards relative to the stem, the color after the pollination is dark green, the anthocyanin discolouration of the sheath is very weak, the leaf envelope has a mild, leafy fluffy medium, the foliage of the leaf envelope is moderate the number of ribs is some, the angle between the crankshaft and the branch is large and the anthocyanin discoloration of the ports is poor. ffl HU 209 665 Description range: 8 pages (including 2 sheets)

Description

FIELD OF THE INVENTION The present invention relates to a female inbred line of the breed DE 8017.

Zea mays L., or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize cannot be commercially grown, they are of particular importance in the production of first-generation (FJ hybrids, for example, hybridization of two inbred lines. contrast enhanced yields, strong stem and root, homogeneity, as well as insects and diseases has shown a strong resistance. másodgenerációjú selfing or cross pollination (F ₂₎ hybrids F! hybrid plants produced. the F ₂ hybrid plants and seeds properties less favorable than the F] hybrid traits, such as lower yields and undesirable genetic traits. Seeds of Fj hybrids resulting in a slender second generation F ₂ hybrid are not harvested by growers. On the contrary, growers expect each year new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (Fj) hybrid seeds.

The production and selection of inbreeding F lines, which result in the crossing of first order F hybrids, is a specialized and highly specialized task. The function of the breeder is not only to produce and select first-order inbreeding lines, but also to produce hybrid combinations that are F! hybrids and thus commercially available.

Due to the important role of inbred lineages in the production of commercial Fj maize hybrids, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. It has become necessary to develop new inbred lines that produce first-generation hybrid maize with higher yields, greater stability and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line DE 8017.

It is an object of the present invention to provide an inbred line of the breed DE 8017 which, when crossed with another inbred corn as a female or male parent, produces a first generation (FJ) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, an inbred corn line DE 8017 is used which has high yield, excellent stalk and excellent greening properties.

The inborn maize line DE 8017 of the present invention can be used to produce hybrid maize, wherein a first corn line is crossed with a second corn line, wherein the first maize line or second corn line is the inbred maize line DE 8017 or the other.

The inbred line DE 8017 of the present invention, when used as a lineage, can be crossed with other maize lines, preferably inbreeding maize lines, to produce first generation (FJ maize hybrids).

DE 8017 inbred corn line is a yellow horse-bred inbred line and its development is described below:

In the winter of 1978-79 in Kihei we cross-pollinated between versions P 3901 and J 61, for which pollen 138-77. lines were manually transferred to line 149-100. line. The inbreeding line J 61 is derived from the hybrid P 3901 by self-pollination and selection.

In summer 1979, Olivia (MN, USA) developed a population 26-28 containing a particularly valuable block in area 16. self-pollinating on row 70 and individual plants and harvesting 70 tubes from 72 plants.

In the winter of 1979-80, in Kihei (Maui), the 70 selected S) tubes were grown in separated S ₂ rows from areas 1-15 of area 26. and Areas 27 through 1-55. line. IB 18 traces back to hit the _S2 generation 27-37. line.

Olivia in (MN) in the summer of 1980 to transition into ΓΒ 18 S ₂ S ₃ generations of selection were grown in the 36th row of the area No. 7.

In the winter of 1980-81 no further cultivation was carried out and the seeds were stored.

HU 209 665 B

In the summer of 1981 we selected three pipe in Olivia (MN), the generation S _3, and S ₄ generations per tube on a separate line grown in the area No. 56 35-37. line. 56/35. line was chosen as the basis for the S ₅ generation.

In the summer of 1982, three selected tubes were planted in Olivia (MN, USA). 70-72. line. A11 / 71st line was selected as the basis for the S ₆ generation. In the winter of 1982, line 12 of line 82 was further propagated and the tubes harvested. The new inbred line received the IB 18 mark on 15.12.1982.

The line is then denoted by code DE 8017.

The DE 8017 line was distinguishable, uniform and constant in respect of the properties tested.

The DE 8017 inbred line is the property of Dekalb Plant Genetics and was not accessible to users.

Cells of plants of the inbred line DE 8017, which can be cultured in an appropriate culture, then separated and regenerated to produce plants of the inbred line, are within the scope of the present invention. Details of the generation process are described in C. E. Green and C. A. Rhoes, Plant Regeneration is Tissue Culture of Maize, Maize Foor Biological Research, W. F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 8017 is a yellow horse-toothed corn, which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for the Conduct of Tests for Distinctness, Homogeneity and Stability)

Maize, November 12, 1980).

Coat of arms, beginning of flowering: medium

Coat of arms, anthocyanin coloration of anthers: weak

Tube, mustache appearance (50% of plants): medium

Anthocyanin coloration of tube, mustache: medium

Anthocyanin coloration of coat of arms, chaff: none or very weak

Coat of arms, anthocyanin ring at the base of the husk: none

Anthocyanin coloration of stems, nodules: none or very weak

Anthocyanin coloration of stems: no v. very weak

Anthocyanin coloration of stems, nodules (in the center of the plant): none or very weak

Leaf angle to stem: It is upwards

Leaf, number of longitudinal ribs: a few

Leaf, hairs on the brim: medium

Leaf, enveloping medium: medium

Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): Large

Coat of arms, number of primary side branches: medium

Coat of arms, crankshaft length is the lowest above the side: Coat of arms, crankshaft length at top medium over song: medium Root, color of the supporting roots: red Angle between crankshaft and junction: large Anthocyanin coloration of anthers: weak Plant, height (incl. Coat of arms): Pipe, tube fitting knot medium relative to total prescription height: medium Pipe, handle length: medium Pipe, length: short Tube diameter (center of tube): medium Tube Shape: Slightly conical Pipe, number of cores: Pipe, type of cores (middle of pipe) medium third): dent Tube, core tip color: yellow Tube, core backside color: orange Tube, chaff anthony coloration: Tube, chaff anthony coloration- there is intensity: medium. Figures 1 and 2 show a female inbreeding breed of DE 8017

of maize line no. Fig. 3A shows the tube, seed and cot.

The inbred maize line DE 8017 is a yellow horse-bred inbred line derived from the cross of the hybrid P 390lxJ 61 with the inbred line. The closest known maize line is DE 8016. The inbred line DE 8017 differs significantly from the known inbred line DE 8016 in plant height (179.2 cm and 155.8 cm) and tube height (83.6 cm and 59 cm respectively). , 1 cm), anthocyanin coloration of the stalk (none or basic or weak), the color of the supporting roots (red or green), the leaf angle (upwards or middle), the color of the leaf after pollination (dark green or medium green) , leaf width (8.9 cm and 7.2 cm, respectively), anthocyanin coloration of the leaf envelope (none or base or weak), foliage of the leaf envelope (medium or strong), number of longitudinal ribs (few or none) , at the crest the angles of the crankshaft and side branches (open and middle), the color of the anthers (pink and red) and the length of the stalk (11.2 cm and 15.4 cm, respectively).

The above characteristics of the inbred line DE 8017 relative to the known inbred line DE 8016 are summarized in Table 1:

Table 1

specific DE 8017 DE 8016 THE B plant height (Cm) 179.2 155.8 40.186 12.4 pipe fitting height (cm) 83.6 59.1 26.376 10.1 anthocyanin coloration of the stem no weak base color of supporting roots red green

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specific DE 8017 DE 8016 THE B leaf angle up center position color after pollination dark green medium green leaf width (cm) 8.9 7.2 0.118 0.7 anthocyanin coloration of the envelope no basis weak envelope fluffiness medium strong number of longitudinal ribs few no crankshaft and side branch angle open middle position color of ports pink red stem length (cm) 11.2 15.4 2.414 3.0

A: squared error average

B: standard deviation, significance - 0.05

The isozyme analysis of the inbred lines DE 8017 and DE 8016 shows that there are three genetic divergences:

Acphl = 2 instead of 4,

Got2 = 4 instead of 2,

MdhA = 1 instead of 6.

The isozyme genotype of the inbred line DE 8017 is compared with the known inbred line DE 8016 in Table 2:

Table 2

Presence of Uelomorph

Place DE 8017 DE 8016 Acphl 2 4 adhl 4 4 Cat3 9 9 Got3 4 4 Got2 4 2 Gotl 4 4 Glu B 6 6 ldhA 4 4 IdhB 6 6 MdhA 1 6 or 0 MdhB 3.5 3.5 MdhC 16 16 MdhD 12 12 MdhE 12 12 Pgm A 9 9 6 PgdA 3.8 3.8 6-Pgd B 5 5 Phi 4 4 number of plants tested: 6 6

Technique for determination of isozyme genotype by Goodman man. N. and Stuber, C.W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophopresis, Proceedings of the Thirty-fifth Annual Com and Sorghum Industries Research Conference, 1980.

The inbred line DE 8017 is preferably used as a parent line for the production of hybrid maize, especially first generation (F,) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the DE 8017 inbred line, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or other agrochemicals for proper development.

During flowering, when the inbred line DE 8017 is used as a female parent unit, the coat of arms of the DE 8017 maize line is removed from all plants. This removal can be done manually or mechanically.

Female parental units can be chemically sterilized or concomitantly administered with a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female lineage, all pollen from the male lineage is suitable for pollination since all the plants of the inbred lineage, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known in the art.

After that, both parent lines are further cultivated until maturity, but only tubes from female parent units are harvested for the new F! for harvesting seeds of hybrid maize. Seeds from the male parent unit can also be harvested and used as needed, but these seeds are not suitable as hybrid seeds.

The resulting new Fj hybrid seeds can be planted next season when F | hybrid maize plants develop.

The inbred line DE 8017 is suitable for the production of new F maize plants which have advantageous properties, for example, the inbred line DE 8017 is used as a female parent line and DE 0802 is an inbred line as a male parent line. The first generation (T ?!) hybrid received the DK EXP 942 mark.

The first generation hybrid (F ^ DK EXP 942) has similar characteristics to the commercial Pio4

HU 209,665 Β neer 3925 and the Mirna variety. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3

EXP 858 and DK 547

Hybrid crop (Bushel) * Magned- vesség- content (%) Pipe Loss (%) Knee deflection (%) Root- roll (%) DK EXP 942 129.4 20.9 0.2 2.9 1.6 Pioneer 3925 119.9 20.4 0.6 2.6 1.7 XX XX XX

number of places: 168 x = statistically significant at 5% xx = statistically significant at 1% + = statistically significant at 10%.

* 1 bush: 36.35 liters.

It can be seen from the foregoing that the inbred line DE 8017 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous characteristics.

Claims (1)

DE 8017 inbred maize line obtained by crossing hybrid P 3901xJ 61 with inbred line having medium onset of flowering, medium onset of mustache, medium anthocyanin coloration of mustache, anthocyanin coloration of chaff, absent at the base, anthocyanin coloration of the nodes, anthocyanin coloration of the nodes, anthocyanin coloration of the nodules, lack of fluff at the periphery of the mantle medium, the length of the crankshaft at the middle of the medial , the length of the tube is medium, the tube is short, the diameter is medium and slightly conical, the number of seeds is medium, the seed type is equine, the apex of the seed is yellow, the backside is orange and the anthocyanin coloration is medium, the plant height is medium, the tubing height is medium, the anthocyanin color of the stem is absent or very poor, the roots of the stalk are red, the angle of the leaf is upward to the stem, the leaf color after pollination is dark green, the anthocyanin color of the , the foliage of the leaf envelope is medium, the number of longitudinal ribs are few, the angle of the crankshaft and the lateral branch are large, and the anthocyanin coloration of the anthers is poor.