HU212516B - De 6175 variety of inbred male corn line (zea mays l.) - Google Patents

Abstract

FIELD OF THE INVENTION The invention relates to an inbred maize line DE6175 derived from the crossing of MO17HxSK16 lines, where the appearance of the leaf slightly bends, the beginning of flowering is medium, the anthocyanin discolouration of the pores is missing or very weak, the appearance of the mustache is medium, the anthocyanin discoloration of the chaff is absent or very poor. weak, the anthocyanin discoloration of the nodes is missing or very weak, the anthocyanin discolouration of the nodes is weak, the anthocyanin discoloration of the envelope is weak, the edge of the envelope is slightly hairy, the branching of the coat of arms is heavily bent, the number of junctions is short, the main axis of the coat of arms is short and the crest of the coat is short and short medium top, the tube shape slightly conical, long handle, the core type is smooth, the tip of the core is orange, orange on the back and the anthocyanin discolouration of the chaff is missing, where the height of the plant is 170-185 c m, the tube height is 60-70 cm, the anthocyanin staining of the stem is weak, the leaf angle is 13-16, the color after the pollination is moderately green, the leaf wind is wavy, the length of the wrinkles is large, the coat length is 32-35 cm, the length of the coat is 32-35 cm. length of the coat of arms is 22-25 cm, the length of the coat of arms is 11-14 cm, the angle of the junction of the coat of arms is large, the anthocyanic ring is missing at the base of the chaff, the anthocyanic discoloration of the mustache is medium, the position of the tube is standing, the length of the tube is 13-17 cm, the tube diameter of 3.5 to 5 cm, the tube shell is long, the number of core lines is 13 to 16, the number of seeds per row is 24 to 28, the length of the core is 10 to 13 mm, the width of the core is 8 to 11 mm and the thickness is 5-7 mm. EN 212 516 B Scope of the description: 6 pages (within / page figure)

Description

The present invention relates to a male inbred line of the variety DE 6175.

Zea mays, or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research are not commercially grown, they are of particular importance in the production of first-generation (Fi) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hybrid viability or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of Fj hybrid plants produces second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the Fj hybrid, thus yielding lower and undesirable genetic characteristics. Due to the reduced characteristics of the less preferred resulting second generation hybrids Fj F ₂ hybrid seeds not harvested by growers. On the contrary, every year growers expect new hybrid seeds obtained by crossing selected first-generation (F0 hybrid seed) inbred parent stock.

Crossing is the first order of F! The production and selection of inbred lines resulting in hybrids is a specialized and highly specialized task. The task of the grower is not only to produce and select first-order inbreeding lines, but also to produce hybrid combinations that are F | hybrids and thus commercially available.

Due to the important role of inbred lineages in the production of commercial F1 maize hybrids, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line of the variety DE6175.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line of the genus DE6175 which, when crossed with other inbred maize as a parent or female, produces a first generation (Fi) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, an inbred line of the variety DE6175 is used which has high yield, excellent stalk and excellent greening properties.

The inbred maize line DE6175 of the present invention can be used to produce hybrid maize, wherein a first maize line is crossed with a second maize line, wherein the first maize line or second maize line is a differentiated maize line and a second maize line.

The new inbred line of the present invention has a variety denomination DE6175, a research designation MOSK1716B2, and a French designation SK124. The lineage of the present invention, when used as a lineage, can be crossed with other maize lines, preferably inbreeding maize lines, to form first generation (F ₍ ) maize hybrids).

The inbred maize line DE 6175 is an orange smooth-seeded inbred line and its development is described below:

The DE6175 variety line originates from the crossing of the M017H and SK16 lines. Line M017H is a variant of line MO 17 resistant to Helminthosporium turcicum, as disclosed in DekalbAg. Research developed from the well-known MO17 line at the University of Missouri, USA. SK16 is owned by Sockalb, France. The original MO17HxSK16 crossing was performed at the request of D. Segonds in Mason (Michigan, USA) in the summer of 1978. The seeds of some early late crosses, which included the MO17HxSK16, MO17HxCM88 and M017xCGll crosses, were sent to Sockalb (France), where self-pollination was performed in the summer of 1979. Crossing of MO17HxSKl6 a

HU 212516 Β

It was in line 6189. The S | Generation I seeds were harvested and a herd was sent to D. Segonds (Druelle, France).

In the summer of 1980, S! Generation I seeds were planted in Druelle (lines 1375-1379). We harvested 23 selfed tube, shelled, and S ₂ generation seeds collected and stored separately.

In summer 1981, 9 of the 23 tubes were set aside and the remaining 14 were planted with 24 selected self-pollinated tubes. 21 808 lines (MO17HxSK16 + 4, were harvested and shelled one selfed tube separately. Maintained S _3rd generation seeds. Summer 1982 S ₃ generation seeds Druelle was planted (30 348 lines). Harvested two selfed tube shelled separately and _S4 generation seeds were stored separately.

In the summer of 1983, the S ₄ generation seeds of the second tube were planted in Druelle (line 40,386). The DE1675 pedigree in the S ₄ generation is 4-1-2. We harvested two selfed tube, shelled, and S ₅ generation seeds collected and stored separately.

In the summer of 1984 the first pipe ₅ S Druelle generation seeds were sown (50 146 lines), and are shown in the MOSK1716B2-1 code. A piece of self-pollinated tubing was harvested and stored.

In the summer of 1985, two rows of the above beak were planted in Druelle, 9 self-pollinated tubes were harvested, separately crushed and stored.

Since the summer of 1986, the pure source of DE6175 has been maintained by self-pollination and storage of selected tubes. Code MOSK1716B2-1 was changed to SK124 when the line was removed from the research department.

Over time, the DE6175 strain has been shown to be phenotypically and genetically stable. The line of variety denomination ADE6175 is uniform with respect to the properties tested. The DE6175 variety was recognized by the French Ministry of Agriculture in January 1990 as uniform, stable and distinct with respect to the other maize lines.

DE6175 is owned by G. I. E. Sockalb in France and Dekalb Plant Genetics in other countries. DE6175 variety line was not available to users.

Cells of the inbred line of DE6175, which can be cultured in an appropriate culture, then separated and regenerated to produce the inbred line, are within the scope of the present invention. Details of the generation process are described in C.E. Green and C.A. Rhoes, Plant Regeneration in Tissue Culture of Maize, Maize Foor Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the DE6175 inbred line is orange plain corn, which is preferably adapted for growing in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability of Maize, November 12, 1980):

Leaf, appearance (middle third of plant):

slightly sloping

Coat of arms, beginning of flowering: medium

Coat of arms, anthocyanin coloration of anthers: none or very weak

Appearance of maize mustache (50% of plants): medium

Anthocyanin coloration of maize mustache: medium Coat of arms, anthocyanin coloration of chaffinch: none or very weak

Coat of arms, anthocyanin ring at the base of the chaff: none Stem, anthocyanin coloration: none or very weak

Stems, anthocyanin coloration of nodules (center of plant): weak

Anthocyanin color of leaf, envelope (in the center of plant): weak

Leaf, hairs on the brim: weak

Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): Large

Coat of arms, appearance of branches: strongly folded Coat of arms, number of branches: little Coat of arms, length of crankshaft above lower branch: short Coat of arms, length of crankshaft above upper branch: medium

Plant height (incl. Coat of arms): medium Pipe, tube fitting relative to total plant height: medium

Tube, handle length: long

Tube, length: medium

Tube diameter (center of tube): Thick

Tube Shape: Slightly conical

Pipe, number of cores: medium

Tube, type of cores (middle third of tube): smooth-grained

Tube color, orange color

Tube, core backside color: orange

Tube, chaff anthocyanin coloration: missing Tube, chaff anthocyanin coloration: very weak

The inbred maize line DE6175 is an orange smooth-eyed inbred line derived from the MO17HxSK16 cross. The closest known corn line is SK16. The DE6175 inbred line is statistically significantly different from the known SK16 inbred line at plant height (177.4 cm and 126.1 cm, respectively), tube height (65.9 cm and 43.1 cm, respectively), and stalk anthocyanin coloration. (weak or strong), in leaf angle (24.5 "and 47.0 '), in color after leaf pollination (medium green or dark green), in leaf folds (many or few), in leaf longitudinal folds (many or few), at the height of the tassel (33.5 cm and 22.0 cm), at the length of the crest (23.9 cm and 17.0 cm), and at the length of the tassel (12.1 cm and 6.7 cm), at the angle of the crest of the crest (open or medium), the anthocyanin ring at the base of the chamois (none), the color of the hair (red or light tan), the position of the tube (upright or horizontal), the length of the pipe3

EN 212 516 Β (15 cm and 12 cm, respectively), tube diameter (4.1 cm and 3.1 cm), length of leaf (long and medium), number of cores (14.4 and 17 cm, respectively). , 0), 26.2 and 20.5, respectively, in the number of seeds per row, 11.8 mm and 7.8 mm respectively, and 9.5 mm and 6.5 mm respectively, and in core thickness (6.2 mm and 3.5 mm, respectively).

The above characteristics of the inbred line DE6175 relative to the known SK16 inbred line are summarized in Table 1:

Table 1

specific DE6175 SK16 THE B plant height (cm) 177.4 126.1 57.305 15.0 tube height (cm) 65.9 43.1 33.640 11.5 anthocyanin coloration of the stem weak strong leaf angle 24.5 ' 47.0 ' leaf color after pollination medium dark green green leaflet wrinkles many some longitudinal folds of leaf many some coat of arms height (cm) 33.5 22.0 4.623 4.3 crest length (cm) 23.9 17.0 1,161 2.9 coat of arms length (cm) 12.1 6.7 .941 1.9 angle of branch of crest open a closer pes anthocyanin ring at the base of the chaff no there is mustache color red cserszí- Nu tube position erect water- storey tube length (cm) 15.0 12.0 .593 1.5 pipe diameter (cm) 4.1 3.1 0,026 0.3 length of leaf long a closer pes number of cores 14.4 17.0 0.504 1.4 number of core lines per line 26.2 20.5 4.796 4.3 core length (mm) 11.8 7.8 0.212 0.9 core width (mm) 9.5 6.5 0,240 1.0 core thickness (mm) 6.2 3.5 0.194 0.9

An isozyme analysis of the inbred lines DE6175 and SK16 shows that there are three genetic differences: Acphal = 2 instead of 4, B-Glul = 6 instead of 7, Pgm-2 = 8 instead of 4.

The isozyme genotype of the inbred line DE6175 is compared to the known inbred line SK16 in Table 2.

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Com and Sorghum Industry Research Conference, 1980.

Table 2

Place Allelomorph presence DE6175 SK16 Acphl 2 4 adhl 4 4 Cat3 9 9 Diai 8 8 slide2 4 4 Got3 4 4 Got2 4 4 Gotl 4 4 B-Glul 6 7 Idhl 4 4 ldh2 4 4 Mdhl 6+ 6+ Mdh2 6 6 Mdh3 16 16 Mdh4 12 12 Mdh5 12 12 Pgm-1 9 9 Pgm-2 8 4 6 Pgdl 3.8 3.8 6 Pgd2 5 5 Phi 4 4 number of plants tested 6 6

notation: + = presumably 6, but 0 cannot be excluded

The inbred line DE6175 is preferably used as a parent line for the production of hybrid maize, especially for the first generation (FJ hybrid maize. When used, this inbred line is used in combination with another inbred line and hybridized to the conventional line by two lines. the inbred line DE6175 is planted in close proximity to each other for pollination, this can be done by planting the lineages in alternating rows, blocks or by any other means The plants of the two lines are cultivated until flowering. regularly treated with fertilizers and / or other agricultural chemicals for proper development.

During flowering, if the inbred line DE6175 is used as the male parent, the coat of arms of the other parent is used for all plants4

HU 212 516 Β removed. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female parent line, only the pollen from the male parent line is suitable for pollination since all the plants of the inbred line, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parental lines are reared to maturity, but only from female parental units. tubes are harvested to harvest the seeds of the new F ₍ hybrid maize). If necessary, seeds from the male parent unit can be harvested and used, but these seeds are not suitable as hybrid seeds.

The resulting new Fj hybrid seeds can be planted next season, when high yield F1 hybrid maize plants will develop.

Inbreeding line ADE6175 new F! corn plants, which plants have their advantageous properties, for example, the inbred line DE6175 is used as the male parent line and DE8016 inbred line is used as the female parent line. The first generation (F0 hybrid received the DK300). The characteristics of the DK300 hybrid were compared to those of the commercially available DEA variant. Both variants show similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3 DK300 and DEA

Hybrid crop (Bu) Magned- vesség- content (%) Knee deflection (%) Root- roll (%) Flowering time (days) DK300 121.2 34.0 2.8 1.1 94.7 BUT THE 109.4 32.8 2.1 7.9 90.1 XX XX XX XX

number of places: 916 xx = statistically significant at 1% level

It can be seen from the foregoing that the inbred line DE6175 provides a preferred breeding strain when used as a male or female lineage to produce first generation hybrids with particular and advantageous properties.

Although the invention has been described in detail and in all respects, it will be apparent to those skilled in the art that various changes and modifications are possible without departing from the spirit and scope of the invention.

Claims (1)

Patent Claim Point Inbred maize line DE6175, derived from the crossing of MO17HxSK16, with a slight deflection of the leaf, medium onset of flowering, no or very low anthocyanin coloration of the anthers, medium onset of whiskers, a very slight lack of anthocyanin, weak anthocyanin coloration, weak anthocyanin coloration, slightly hairy at the periphery of the sheath, strongly dorsal at the crest, few branches, short crest at mid-crest and slightly conical at upper crest, , the seed type is smooth-eyed, the apex of the seed is orange, the backside is orange and the anthocyanin coloration of the chaff is absent, characterized in that the height of the plant is 170185 cm, preferably 177.4 cm, the height of the tube is 6070 cm, preferably 65.9 cm, the stem is anthoci faint coloration of the stamen, leaf angle 13-16, preferably 14.5, medium color after pollination, leaf wavy wavy, long elongated wrinkles, coat of arms 32-35 cm, preferably 33.5 cm, crest length 22-25 cm, preferably 23.9 cm, coat of arms 11-14 cm, preferably 12.1 cm, large angle of branching of the coat of arms, absence of anthocyanic ring at the base of the pericarp, medium anthocyanin coloration of mustache , tube length 13-17 cm, preferably 15 cm, tube diameter 3.5-5 cm, preferably 4.1 cm, tube length long, number of seeds 13-16, preferably 14.4, number of seeds per row 24-28, preferably 26.2, core length 10-13 mm, preferably 11.8 mm, core width 8-11 mm, preferably 9.5 mm and thickness 5-7 mm, preferably 6.2 mm.