HU202354B - Male inbred maize line of sign 0643 - Google Patents

Abstract

FIELD OF THE INVENTION The male inbred maize line DE0643, produced by the crossing of MDF 13A (H4101) x M017H, whose leaf is bouncing, the beginning of flowering is low, the anthocyanin discoloration of the pores is absent, the appearance of the mustache is moderate, the anthocyanin discoloration of the mustache is poor the anthocyanin discoloration of the chaff is absent, the anthocyanin ring at the base of the chaff is absent, the anthocyanin discoloration of the knots and nodes is absent, the anthocyanin discoloration of the leaf envelope is absent, the edge of the coat of arms is small, the angle of the crankshaft and the lateral branches is small, the side branches fold back, their sides are bent, the cranial folds back, their number is medium, the cranks are bent, the cranks are back, the cranks are backs, the cranks are backside long, the pipe's height is medium, the tube's handle is long, the tube is medium, its diameter is medium, its shape is cylindrical, the number of core lines is a few, the type of seeds is toothed, the color of the core tip is yellow, the back color of the core is yellow, and the back of the core is yellow, and the chaff is slightly anthocyanic. A characteristic feature of the invention is that the number of days between the appearance of the mustache and the 25% moisture content is 55, the height of the plant is 249 cm, the length of the tube is 87 cm, the weight of the tube is 79 g, the anthocyanin coloration of the seedling is absent, the leaf coat has a medium hairiness, longitudinal the number of wrinkles is small, the tube is solid, the color of the seedling is medium green, the stem is anthocyanin discolored and the length of the leaf above the tube is 84 cm. (Figure 1) in co CM o CM Scope of the description: 8 pages, Figure 1 -1-

Description

Scope of the description: 8 pages, Figure 1

EN 202354

The invention relates to DE0643. male inbred maize line.

Zea mays, a corn in many countries around the world, is a major agricultural crop, especially in the United States. Corn is used for food, feed and industrial purposes.

In the past 60 years, hybrid maize has been dominant in trade. As a result of the hybridization, varieties with significantly higher yields, stronger stems and roots, and more uniform characteristics and resistant to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the birthplace. Homozygous character can be achieved by inbreeding within the inbred line, and in general, the inbreeding generation with sixth or seventh self-pollination can be considered genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield, and other plant characteristics. As a result, inbred corn lines cannot be grown for commercial purposes.

Although inbred lines developed by a number of breeding experiments in the field of corn cannot be grown for commercial purposes, they are of particular importance for the production of first generation (Fi) hybrids, for example by defibrillation of two inbred lines.

By crossing two inbred lines, hybrid vigor or heterosis occurs and the hybrid plant has a strong resistance to high yields, strong stems and roots, homogeneity, and insects and diseases. Self-pollination or cross-pollination of Fi hybrid plants produces second-generation (F2) hybrids. The properties of the F ₂ hybrid plant and its seeds are less favorable than those of the Fi hybrid, so yields can be expressed with lower and undesirable genetic characteristics. Due to the reduced characteristics, the seeds of the Fi hybrids that produce less preferred second generation F ₂ hydride are not harvested by growers. On the contrary, growers expect new hybrid seeds to be traded each year by crossing selected inbreeding nurseries for first generation (Fi) hybrid seed.

The production and selection of inbred lines that produce cross-hybrid first-order Fi hybrids is a task that requires special expertise. The task of the grower is not only to produce and select first-order inbred parent lines, but also to produce and select hybrid combinations suitable for the production of Fi hybrids and thus commercial distribution.

Due to the significant role played by inbred lines in the production of commercial maize hybrids, special emphasis has been placed on the development of inbred lines in the field of maize production and hybridization. Thus, it has become necessary to develop new inbred lines that yield higher yields during hybridization2 with first-generation hybrid maize with greater stability and better resistance. A further requirement is the homogeneity of first generation hybrids, which facilitates mechanical harvesting and reduces labor demand.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a novel inbred maize line DE0643.

It is an object of the present invention to provide an inbred line DE0643, characterized in that, if it is crossed with other inbred maize as a female or male parent, a first generation (F) hybrid with advantageous characteristics is obtained.

With the inoculum of the inbred line DE0643 according to the invention, other maize varieties are fertilized to produce high quality first generation (Fi) maize hybrid.

The DE0643 inbred line according to the invention can be used to produce a hybrid, which results in first-generation (Fi) hybrid maize having good properties as a result of heterosexuality, such as high yields, good stability, insects and diseases.

Thus, in accordance with the invention, the inbred maize line DE0643 is used, which has a high yield, excellent stem and excellent greening properties.

The hybrid maize according to the invention is prepared by crossing the first corn line with a second corn line, where the first maize line or the second maize line is the inbred maize line DE0643, and the other line is a different inbred corn line or cross-hybrid maize.

In the production of hybrid maize varieties we do so

(a) inoculating the seeds from inbred lines DE0643 and seeds from other maize lines;

(b) the plants developed as a result of planting are grown until flowering;

c) irrigating one of the maize lines;

d) performing cross-pollination between the inbred line DE0643 and the other maize line; and

e) harvesting seeds of plants of the other corn line, wherein the other corn line may be hybrid maize crossing or an inbred corn line which is preferably not the same as DE0643.

The inbred line DE0643 according to the invention, used as a parent line, can be crossed with other corn lines, preferably inbred maize lines, which produce first-generation (F) maize hybrids. Although the inbred line DE0643 is generally used as a male entity during crossing, it can also be used as a female.

EN 202354

The inbred maize line DE0643 is a yellow horse-toothed inbred corn line and is developed as follows:

MDF 13A (H4101) X M017H (version M017 Ht == 187-2xC103) crossings were made in Dayton, Iowa. We gathered the SO generation seeds of the harvested tubes (plantation size 1592x1588, MDG-13a line: 1592, row M017H: 1588), and then sent for self-polling to Homestead, Florida. All harvested tubes were returned to Dayton, dropped in Iowa, and Sí generation seeds were collected (Plantation Serial No. 1900). They were planted in a row in Danyton, Iowa, and then self-pollinated on plants. Three self-pollinated tubes (S2 generation cores) were harvested, individually peeled and S2 seeds stored separately (Plantation Line 4770).

S2 generation seeds from one of the three harvested tubes were planted in an eight-line block in Homestead, Florida, and the plants were grown for self-pollination. Eleven self-pollinated tubes were collected and returned to Dayton, Iwoa. The tubes were discarded separately and S3 generation seeds were stored separately (plantation line: 1007-1014). Of the eleven harvested tubes, sixth S3 generation seeds were planted in Dayton, Iowa, in a two-row block, and the plants were grown for self-pollination. Eight self-pollinated tubes were collected, discarded separately, and S4 generation seeds were stored separately (plantation line: 4161-4162).

Out of the eight collected tubes, the sixth S4 generation seeds were planted in Homestead, Florida, and grown to self-pollinate. A self-pollinated tube was collected and returned to Dayton, Iowa. S5 generation seeds were crushed and stored (plantation line: 219). The single-seed S5-generation seeds were planted in Dayton, Iowa, and were grown for self-pollination. Four self-pollinated tubes were collected, discarded separately, and S6 generation seeds were stored separately (plantation line 3235). The first cores of the four tubes were coded 78060A. The complete self-pollination of 78060A for the S6 generation is S6-1-6-6-1-1. The seeds of the 78060A S6 generation were planted in Hawaii and grown for self-pollination. Twelve-three tubes were collected, returned to Dayton, Iowa, and crushed seeds were harvested (Plantation Line 1797). The pure origin of DE0643 is maintained by self-pollination and accumulation of seeds from selected tubes for all generations. This line was assigned the code DE0643.

The DE0643 inbred line is homogeneous apart from the height of the plant, with some variations in height, a constant feature of the line.

The DE0643 inbred line is owned by DEKALB-PFIZER GENETICS, which was not accessible to users.

The seeds of the inbred line, which was developed in the well-known inbreeding, have been deposited with the French Ministry of Agriculture at the General Secretariat of the Committee for the Protection of New Varieties (17th Avenue de Tousville, 75007 Paris, France).

The cells of the inbred line DE0643 according to the invention, which can be cultivated in a suitable culture, can be separated and regenerated, producing the plants of the inbred line. Details of the generation process

C. E. Green and C.A. Rhodes: Plan Regeneration In Tissue Culture of Bread; Maize Forum Biological Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlotsville, USA, 367-372 (1982).

As mentioned above, the inbred line DE0643 is a yellow-toothed corn, which can be adapted to cultivation in the northern corn zone of the United States of America.

This inbred line has the following features (UPOV: Guidelines for the Conduct of Tests Forum Distinctness, Homogenety and Stability Forum Maize, 12.11.1980):

Leaf, standing (in the central third of the plant): bends back

Coat of arms, beginning of flowering: medium

Coat of arms, anthocyanin discolouration: none or very weak

Tube, hair appearance (50% of plants) medium

Tube, hair anthocyanin discoloration: weak

Tube, flake anthocyanin discoloration: none or very weak

Coat of arms, anthocyanin ring at the base of the chaff: there Stems, anthocyanin discoloration: no or very weak

Stem, anthocyanin discolouration of the nodes (in the center of the plant): none or very weak

Leaf, anthocyanin discolouration (in the center of the plant): none or very weak

Leaf, hair on the edge of the shell: weak

Coat of arms, crankshaft and side angles (in the lower third of the coat of arms): small

Coat of arms, position of side branches (in the lower third of the coat of arms): bend back

Coat of arms, number of primary branches: medium Coat of arms, crankshaft length at the bottom side:

long

Plant, height (with coat of arms): very long Pipe, pipe fitting location relative to plant height: medium

Pipe, handle length: long

Tube, length: medium

Tube, diameter (center of pipe): medium

Tube, shape: cylindrical

Tube, number of lines: few

Tube, core type (in the middle of the tube):

dent

Tube, core tip color: yellow

Tube, core back color: yellow

Tube, the anthocyanin coloration of the chaff: is

Pipe, anthocyanin staining intensity of the chaff: weak.

The inbred corn line DE0643 is a yellow horseshoe inbred corn line. The nearest known corn line is MO17Ht. MO17Ht brings the inbred line to the public3

EN 202354 Β available inbred line MO17, was developed by cross-intersecting the Ht gene that confers resistance to Helminthosporium turcicum. The inbred line 0643 is statistically significantly different from the well-known inbred line MO17Ht from the 50% hair appearance to the days at 25% moisture (55 and 67 respectively) at the height of the plant (249 cm and 230 cm). tube length (87 cm and 75 cm respectively) and coloring of the seedling anthocyanin (absent or present). A further distinctive feature is that the weight of the tube is less than that

MO17Ht.

The above characteristics of the inbred line DE0643 compared to the well-known MO17Ht inbred line are shown in Table 1 in the autumn:

Table 1

specific DE0643 MO17Ht Hyposynthesis of measurement H _o : Mui-Muj HiiMui Ψ Mu2 First Days between 50% Hair and 25% Moisture 55 67 * Second plant height (cm) 249 230 * Third leaf coat hairiness medium mild * 4th longitudinal wrinkle some many * 5th tube length (cm) 87 75 * 6th pipe weight (g) 79 85 * 7th tube strength strong weak * 8th seedling color medium green light green * 9th coloration of the anthocyanin seedling no there is * 10th stem anthocyanin discoloration no basic * 11th Length of pipe over the pipe (cm) 84 69 *

♦ statistically significant at 0.1%

The isozyme analysis of the inbred lines DE0643 and MO17 shows that there are genetic differences between the two different sites, instead of Acpl-3.

Ampl-4 instead of 5.

The isozyme genotype of the inbred line DE0643 is compared with the known MO17 inbred line in Table 2.

Table 2

Place Allelomorpf presence DE0643 MO17 Number of plants examined 24 24 ACPL 3 2 acoli 4 4 ak 4 4 adhl 4 4 Ample 4 5 Cat 9 9 Diai 8 8 slide2 4 4 Enplo 6 6 Got3 4 4 Got2 4 4 Gotl 4 4 Glul 6 6 Hex2 2 2 Idhl 4 4 Idh2 4 4 Mdhl 6 * 6 * Mdh2 6 6 Mdh3 16 16

Place Allelomorpf presence DE0643 MO17 Mdh4 12 12 Mdh5 12 12 pgml 9 9 Pgm2 8 8 Pgdl 3.8 3.8 Pgd2 5 5 Phi 4 4 Tpil 4 4 Tpi2 4 4 Tpi3 4 4 Tpi4 4 4

* = allelomorpf is presumably 6 but zero cannot be excluded.

Techniques for determining the isozyme genotype are Goodman, Μ, N. and Stuber, C.W., Genetics indentification of lines and crosses using isoenzyme electrophoresis; Proceeding from the Trirty-Fitfh Annual Research and Sorghum Industry Research Conference, 1980.

The inbred line DE0643 is preferably a hybrid maize as a parent line, especially for the production of first-generation (Fi) hybrid maize. When used, this inbred line is used in conjunction with another ett inbred line, and the two lines are hybridized by conventional techniques. That is, two inbred lines, one of which is the inbred line DE0643, are planted in close proximity to pollination. This can be accomplished by placing parent lines in alternating rows,

EN 202354 Β blocks or in any other way. The plants of the two lines are grown until flowering. In the developmental period, the plants are preferably routinely thinned in the three-leaf condition and regularly treated with fertilizers and / or other agricultural chemicals for proper development.

During flowering, when the inbred line DE0643 is used as a male birthplace, the coat of arms of the other birthplace is removed from each plant. This removal can be done manually or mechanically.

Female females may also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated while in the grasses as usual, winds are pollinated by wind. As a result of the neutering of the female parent line all pollen from the male parent line is suitable for pollination, since the coat of arms of all inbred lines, and thus the pollen carrier, which would have played a female role in hybridization, was removed. During the hybridization process, the parent colonies are cultivated separately from corn fields to avoid undesirable entry of pollen from alien species. The required separation techniques are well known to those skilled in the art.

Both parent lines are then further developed until ripening, but only tubes from female females are harvested to collect seeds of the new Fi hybrid corn. If necessary, the seeds of the male parent may also be harvested and used, but these seeds are not suitable as hybrid seeds.

The new Fi hybrid seeds can be planted next season, when high-yield Fi hybrid maize plants develop.

The cultured line DE0643 is suitable for the production of new Fi maize plants, which exhibit favorable properties, for example, the inbred line DE0643 is used as male male line, while the commercial inbred line DE0644 is used as female female line. The first generation (Fi) hybrid received the DK524 signal.

The characteristics of the first generation (Fi) DK524 hybrid were compared with the properties of a similar commercial T1000 variant (Dekalb-Pfizer Genetics). Both variants show similar yields, making them particularly suitable for comparison. The results are shown in Table 3.

Table 3

Hybrid DK524 T1000 Product (bush) * 137.4 121.2 * Seed Moisture (t%) 23.0 23.3 * Plant height (cm) 228 197.25 * Pipe Height (cm) 113.25 86.50 * green Ground 96.3 85.9 * The stem does not lie 99.5 100.1 * Moisture content of fruit 114.0 98.9 *

* = 1% statistically significant * 1 bush = 36.35 liters.

From the above it can be seen that the inbred line DE0643 provides a preferred cultivation strain when used as a male or female parent line to produce first-generation hybrids with special and advantageous properties.

Although the invention has been described in detail and with respect to all features, it will be apparent to those skilled in the art that, without departing from the spirit and scope of the invention, various changes and modifications are possible.

Claims (1)

Male inbred line DE0643, produced by intersection of MDF 13A (H4101) x M017H, with a drooping leaf, medium onset of flowering, lack of anthocyanin-anterior θθ coloring of anthers, medium onset of mustache, anthocyanin of mustache , anthocyanin coloration of chaffinch, no anthocyanin ring at the base of the chaff, no anthocyaninic coloration of nodules and knots, no anthocyaninic color of the pericarp, margin slightly hairy, crest of axillary and lateral branches, long, tube has medium height, tube handle is long, tube is medium, diameter is medium, number of seeds is few, seed type is equine, nucleus color is yellow, nucleus backside is yellow, with characterizing that the mustache appears and 25% moisture content, number of days 55, plant height 249 cm, tube length 87 cm, tube weight 79 g, lack of anthocyanin coloration of the seedlings, medium hairiness, few long lines, firm tube, the seedlings have a medium green color, the anthocyanin color of the stalk is absent and the length of the leaf above the tube is 84 cm.