HU210970B - De 6201 variety of male inbred corn line (zea mays l.) - Google Patents

Abstract

FIELD OF THE INVENTION The present invention relates to male inbred maize line DE 6201 derived from the crossing of EPWB X CM 105 lines, where the leaf is heavily folded, early onset of flowering, the anthocyanin discoloration of the pores is absent or very weak, the time of appearance of the mustache is early, and the anthocyanin of the chaff is early. discoloration is missing or very weak, the closed anthocyanin ring is missing at the base of the chaff, the anthocyanin discolouration of the nodes is missing or very weak, the anthocyanin discoloration of the nodes is strong, the anthocyanin discoloration of the leaf envelope is strong, the leaf edge is weak, the angle between the main axis of the coat of arms and the side axes is moderate, \ t the side branches are strongly bent, the number of primary side branches is zero or very little, the main axis of the coat of arms is very short above the lower side, and above the top side, the tube is short, the tube is medium, its shape slightly conical, and the number of core lines is slightly conical s, characterized in that the height of the plant is 135-145 cm, the height of the tube is 3340 cm, the side root is green, the number of leaves is 1317, the color of the leaf after pollination is medium green, the length of the leaf is 60-70 cm, and the width of the leaf is 7 -10 cm, coat length 10-13 cm, length of tube 1114 cm, length of the shell behind the tube is 9-13 cm, the opening of the shell is tight, the anthocyanin discoloration of the cob is missing, the number of seeds per row is 21-25, the seed Type is semi-smooth, the color of the core is orange and the color of the core is orange. EN 210 970 B Scope of the description: 8 pages (including 2 sheets)

Description

The present invention relates to a male inbred line of the breed DE 6201.

Zea mays 1, or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yield, stronger stems and roots and more uniform insect and disease resistance have been developed.

Hybrid maize obtained by simple crossing can be induced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hydride vitality or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. By self-pollinating or cross-pollinating F, hybrid plants produce second-generation (F ₂ ) hybrids. The characteristics of the F ₂ hybrid plant and its seeds are less favorable than those of the Fj hybrid, thus yielding lower yields and undesirable genetic characteristics. Due to the reduced characteristics of the less preferred resulting second generation hybrids Fj F ₂ hybrid seeds not harvested by growers. On the contrary, every year growers expect new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (F,) hybrid seeds.

The production and selection of inbreeding F lines, which result in the crossing of first order F hybrids, is a specialized and highly specialized task. The task of the breeder is not only to produce and select first-order inbred lines, but also to produce hybrid combinations suitable for the production of Fj hybrids and thus to be commercialized.

Due to the important role of inbred lineages in the production of commercial F, maize hydrides, the development of inbred lineages has received special emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

It is therefore an object of the present invention to provide a new inbred maize line of the variety DE 6201.

SUMMARY OF THE INVENTION It is an object of the present invention to provide an inbred line of the variety DE 6201 which, when crossed with other inbred maize as a parent or female, produces a first generation (F,) hybrid with advantageous characteristics.

Thus, in accordance with the present invention, an inbred maize line DE 6201 is used which has a high yield, an excellent stem and excellent greening properties.

The inbred maize line DE 6201 of the present invention can be used to produce hybrid maize, wherein a first maize line is crossed with a second maize line, wherein a first maize line or a second maize line is used to produce an inbred maize line or a second maize line.

As mentioned above, the present invention relates to a new inbred line DE 6201 bearing the mark CMSK2905A2-1 at the research stage, SK 120 in France. The inbred line DE 6201 of the present invention, when used as a parent line, can be crossed with other maize lines, preferably inbred lines, to form first generation (Fj) maize hybrids.

The inbred maize line DE 6201 is a yellow smooth-seeded inbred line and its development is described below:

The DE 6201 line is the result of a cross between the inbred lines EPWB and CM 105. The EPWB line is owned by Sockalb, while the CM 105 line is publicly accessible and has been developed at Morden Station (Canada). The original CM 105 X EPWB lines were crossed by Segonds D. in 1979 in Gaillac (France). S0 generation seeds were planted in Druelle (France) in the summer of 1980 (lines 980 and 981). The Ski Seeds were harvested and the pattern retained for the next generation.

In the summer of 1981, the SI seeds were planted in Druelle (France) (lines 20 801-20 806). Twelve self-pollinated tubes were harvested, individually dropped, and the S2 cores were stored separately.

HU 210 970 Β

In summer 1982, S2 seeds of tube # 1 were planted in Druelle (France) (line 20,617). A self-pollinated tube was harvested, crushed, and the S3 cores stored separately.

In the summer of 1983, S3 seeds were planted in Druelle (France) (line 30 443). Two self-pollinated tubes were harvested, individually stripped, and the S4 cores were stored separately.

In summer 1984, S4 seeds of tube 2 were planted in Druelle (France) (line 40 285). The complete pollination pedigree of DE 6201 is 2-1-1-2. Four self-pollinated tubes were harvested, individually dropped, and the S5 cores were stored separately.

In the summer of 1985, S5 seeds (code CMSK2905A2-1) of tube # 1 were planted in Druelle (France). Three self-pollinated tubes were harvested and the seeded seeds were accumulated.

Since the summer of 1986, the pure origin of the SK 120 has been maintained by self-pollination and by the accumulation of seeds in selected tubes. The CMSK2905A2-1 variety code was changed to SK 120 at the propagation of the line and later to DE 6201.

During the elapsed time, line DE 6201 was maintained by self-pollination. The line has been observed to be phenotypically and genetically stable and uniform in properties. The DE 6201 line was classified by the French Ministry of Agriculture in 1990 as uniform, permanent and distinct.

Inbreeding line DE 6201 in France is owned by G. I. E. Sockalb and, outside France, by Dekalb Plant Genetics, which was not accessible to users.

Cells of plants of the inbred line DE 6201, which can be cultured in a suitable culture, then separated and regenerated to produce plants of the inbred line, are within the scope of the present invention. Details of the generation process are described in C. E. Green and C. A. Rhodes, Plant Regeneraton in Tissue Culture of Maize, Maize for Biological Research, W. F. Sheridan Publisher, Plant Molecular Biology Association, Charlesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 6201 is yellow smooth-grained maize, which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, 12 November 1980):

Leaf, appearance (center of plant)

third): strongly bent Coat of arms, beginning of flowering: Coat of arms, anthocyanin anthers early discoloration: Tube, mustache appearance none or very weak (50% of plants): Tube, mustache anthocyanin early discoloration: none or very weak

Coat of arms, chaff anthocyanin discoloration: you are not very weak Coat of arms, closed anthocyanin ring at the base of the husk: no Anthocyanin coloration of stems, nodules: you are not very weak Anthocyanin coloration of stems, nodules (center of plant): strong Anthocyanin coloration of leaf, envelope (in the center of the plant): strong Letter: edge of leaf poorly hairy Coat of arms, crankshaft and side branches (in the lower third of the coat of arms): medium Coat of arms, appearance of side branches: strongly sloping Coat of arms, number of primary side branches: you are not very weak Coat of arms, crankshaft length above the lowest sidewall: Coat of arms, crankshaft length at the top very short above the side: medium Plant height (including tassel) : short Tube, pipe fitting to the ne I- relative to total prescription height: low Length of tube, peduncle: short Pipe, length: short Tube diameter (center of tube): medium Tube shape: slightly conical Pipe, number of cores: Pipe, type of cores (middle of pipe) few harmadéban) smooth-grained Tube, core tip color: orange Tube, core backside color: Tube, the chaff anthocyanin orange discoloration: no Intensity of tube, the anthocyanin coloration of the husk: very weak

The inbred maize line DE 6201 is a yellow semi-smooth inbred line derived from the crossing of CM 105 x EPWB. The closest known maize line is CM 105. The inbred line DE 6201 is statistically significantly different from the known inbred line CM 105 at plant height (135-145 cm, preferably 140.2 cm and 168.6 cm), at tube height (135 cm). 33-40 cm, preferably 36.8 cm and 56.0 cm, respectively, in the number of nodules with the root (0.5 and 1.4), the color of the root (green and red) and the number of leaves (13- 17, preferably 15.1 on average and 18.0 on average), the color of the leaf after pollination (medium green or dark green), the length of the leaf (60-70 cm, preferably 64.7 cm and 76, 1), in the width of the leaf (7-10 cm, preferably 8.4 cm and 6.9 cm), in the length of the tassel peduncle (10-13 cm, preferably 11.7 cm and 6.4 cm), length (11-14 cm, preferably 12.9 cm and 15.7 cm), the length of the shell behind the tube 3

HU 210 970 Β (9-13 cm, preferably 10.9 cm and 5.9 cm), shell openness (tight or medium), anthocyanin coloration of the cob (none), number of seeds per row ( 21-25, preferably 23.7 on average and 29.8 on average), seed type (semi-smooth or horse-toothed), seed tip color (orange or yellow) and seed side color (orange or brown) ).

The above characteristics of the inbred line DE 6201 relative to the known inbred line DE 8016 are summarized in Table 1:

Table 1

specific DE 6201 CM 105 THE B plant height (cm) 140.2 168.6 57.305 15.0 tube height (cm) 36.8 56.0 33.640 11.5 number of secondary root nodes (pcs average) 0.5 1.4 0,068 0.5 color of the side root green red number of letters (pcs average) 15.1 18.0 0.548 1.5 leaf color after pollination medium- green dark green leaf length (cm) 64.7 76.1 6.401 5.0 leaf width (cm) 8.4 6.9 0.348 1.2 coat of arms length (cm) 11.7 6.4 .941 0.97 tube length (cm) 12.9 15.7 .593 1.5 behind tube casing length (cm) 10.9 5.9 1,040 2.0 Shell openness tight medium anthocyanin coloration of cob no there is number of seeds per row (pcs average) 23.7 29.8 4.796 4.3 seed type semi-smooth dent color of the apex orange- yellow yellow core side color orange- yellow brown

A: Mean square of error

B: Mean standard deviation (significance 0.05.

An isozyme analysis of the inbred lines DE 6201 and CM105 shows no genetic difference between the sites tested.

The isozyme genotype of the inbred line DE 6201 is compared to the known inbred line CM 105 in Table 2.

Technique for determination of isozyme genotype

Google Μ. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Com and Sorghum Industries Research Conference, 1980.

Table 2

Allelomorph presence Place CMSK2905A2-1 CM 105 Acphl 4 4 ADH1 4 4 Cat3 9 9 Gdhl 1 1 Got3 4 4 Got2 4 4 Gotl 4 4 Glu B 7 7 Idhl 4 4 Idh2 6 6 Mdhl 6+ 6+ Mdh2 6 6 Mdh3 16 16 Mdh4 12 12 Mdh5 12 12 pgml 9 9 Pgm2 4 4 Phil 4 4 Number of plants tested 6 6

notation: + = presumably 6, but 0 cannot be excluded

The inbred line DE 6201 is preferably used as a parent line for the production of hybrid maize, in particular first generation (F1) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the inbred line DE 6201, were planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or agrochemicals for proper development.

During flowering, when the inbred line DE 6201 is used as a parent, the coat of arms of the other parent is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of neutering of the female parent line, only pollen from the male parent line is used.

EN 210 970 Β is different for pollination, as the coat of arms of all inbred lines derived from the inbred line, and thus the pollen-bearing part, which would have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from the female parent units are harvested to harvest the seeds of the new Fj hybrid maize. Seeds from the male parent can also be harvested and used as needed, but these seeds are not suitable as hybrids.

The resulting new Fj hybrid seeds can be planted next season, when high yield Fj hybrid maize plants will develop.

The inbred line De 6201 is suitable for the production of new maize plants Fj which have advantageous properties, for example, the inbred line DE 6201 is used as a male parent line and DE 8016 is used as a female parent line. The first generation (Fj) hybrid received the DK232 signal. The characteristics of the DK232 hybrid were compared with those of the commercially available DEA variant. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3 DK232 and DEA

Hybrid crop (Bushels *) Magned- moisture in content (%) Knee deflection (%) Root- roll (%) Flowering time (days) DK232 100.2 32.1 1.7 0.5 94.0 BUT THE 101.9 34.0 3.6 8.3 93.6 X XX X X XX

number of seats: 141 x statistically significant at 5% xx statistically significant at 1% * 1 bus = 36.35 liters

It can be seen from the foregoing that the inbred line DE 6201 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous characteristics.

Claims (1)

Patent Claim Point DE 6201 male inbred line derived from the crossing of lines EPWB x CM 105 with a strongly deflated leaf, early on flowering, no or very low anthocyanin coloration of anthers, early appearance of mustache or anthocyanin coloration very weak, closed anthocyanin ring missing at the base of the chaff, missing or very weak anthocyanin coloration of nodules, strong anthocyanin coloration of leaf nodules, strong anthocyanin coloration of the envelope, weak hairline, midline of crotch and lateral axis strongly drooping, number of primary side branches zero or very low, crest axis very short above the lowest side branch and medium above the uppermost branch, tube peduncle short, tube diameter medium, slightly conical with few nuclei characterized in that the height of the plant is 135-145 cm, preferably 140.2 cm, the height of the tube is 33-40 cm, preferably 36.8 cm, the root is green, the number of leaves is 13-17, preferably 15.1, the color of the leaf after pollination is medium green, the length of the leaf is 60-70 cm, preferably 64.7 cm, the width of the leaf is 7-10 cm, preferably 8.4 cm, the length of the calyx peduncle is 10-13 cm, preferably 11.7 cm , the length of the rain is 11-14 cm, preferably 12.9 cm, the length of the shell behind the tube is 9-13 cm, preferably 10.9 cm, the shell is tight, the anthocyanin coloration of the cob is absent, the number of seeds per row is 21-25 , preferably 23.7 on average, the core type is semi-smooth, the tip of the core is orange and the side of the core is orange.