HU210974B - De 0719 variety of female inbred corn line (zea mays l.) - Google Patents

Abstract

Field of the Invention The present invention relates to an inbred maize line DE 0719 obtained by crossing lines 5B2C-A x DE 0661, where the appearance of the leaf slightly regresses, late in the beginning of flowering, late appearance of the mustache, and the anthocyanin discoloration of the mustache. mild, the closed anthocyanin ring is missing at the base of the chaff, the anthocyanin discoloration of the nodes is mild, the anthocyanin discolouration of the nodes is weak, the angle between the crankshaft and the lateral branches is small, the appearance of the side branches slightly bends, the number of primary side branches is small, the number of primary side branches is small, the crankshaft is the smallest branch, the crankshaft is the lowest branch very short, the length of the crankshaft is short above the uppermost branch, the tube is moderately long, its diameter is medium and slightly conical, the number of core lines is large, the core type is toothed, the tip of the core is yellow, the back is orange and the anthocyanin discoloration is moderate. Its essential properties are that the plant is tall, the height of the tube fitting is high, the anthocyanin discolouration of the stem is weak or absent, the color of the leaf after pollination is medium green, its length is 68-73 cm, preferably 71 cm, width 15-18 cm, preferably 17 cm, the anthocyanin discolouration of the skin is weak, the fluff is strong, the length of the coat of arms is 20-24 cm, preferably 22 cm, the length of the tube edge is 8-12 cm, preferably 10 cm, the anthocyanin coloration of the pores is weak and the caudal strength is weak. CQ EN 210 974 Scope of the description: 8 pages (including 2 sheets)

Description

BACKGROUND OF THE INVENTION The present invention relates to an inbred maize line DE 0719 obtained by crossing lines 5B2C-A x DE 0661 with slight leaf appearance, late onset of flowering, late appearance of mustache, slight anthocyanin coloration of the mustache, slight, closed anthocyanin ring missing at the base of the chaff, slight anthocyanin coloration of nodules, poor anthocyanin coloration of nodes, small angle between crankshaft and side branches, appearance of lateral branches slightly inclined, number of primary lateral branches very short, crankshaft length above the uppermost branch, medium length, medium diameter and slightly conical, large number of kernels, kernel type equine, yellow kernel orange, and anthocyanin coloration medium. Its essential characteristics are that the plant is tall, the tube has a high fitting height, the stems have little or no anthocyanin coloration, the leaves have a medium green color after pollination, length 68-73 cm, preferably 71 cm, width 15-18 cm, preferably 17 cm, the anthocyanin color of the envelope is poor, its pile is strong, the coat of arms is 20-24 cm, preferably 22 cm, the length of the tube handle is 8-12 cm, preferably 10 cm, the anthocyanin color of the anthers is poor and the cob strength is poor.

HU 210 974

Description: 8 pages (including 2 pages)

HU 210974 Β

The present invention relates to a female inbred line of the species DE 0719.

Zea mays L., or corn, is a major crop in many countries around the world, especially in the US. Maize is used for food, feed and industrial purposes.

For the past 60 years, hybrid maize has dominated the trade. As a result of the hybridization, varieties with significantly increased yields, stronger stems and roots, more uniform characteristics and improved resistance to insects and diseases have been developed.

Hybrid maize obtained by simple crossing can be produced by using a homozygous inbred line as the parent unit. The homozygous character can be achieved by inbreeding within the inbred line, and generally the sixth or seventh self-pollinated generation is considered to be genetically pure.

However, inbreeding for essential genetic identity leads to a reduction in resistance, yield and other plant characteristics. As a result, inbred lines of maize may not be grown for commercial purposes.

Although inbred lines developed through numerous breeding experiments in maize research cannot be commercially grown, they are of particular importance in the production of first generation (Fj) hybrids, for example by hybridizing two inbred lines. By crossing two inbred lines, hydride vitality or heterosis occurs, and the hybrid plant has significantly increased yield, strong stem and root, homogeneity, and strong resistance to insects and disease. Self-pollination or cross-pollination of Fj hybrid plants produces second-generation (F ₂ ) hybrids. The F ₂ plants and hybrid seeds properties less favorable than those of the F, hybrid properties, such as lower yields and undesirable genetic characteristics get developed. Due to the reduced characteristics of the less preferred resulting second generation hybrids Fj F ₂ hybrid seeds not harvested by growers. Each year, growers expect new hybrid seeds obtained by crossing selected inbred parental units capable of producing first generation (Fj) hybrid seeds.

The production and selection of inbred lines resulting in crossing Fj hybrids is a specialized and highly specialized task, The breeder is responsible not only for the production and selection of the first hybrids, but also for the hybrid combinations which are commercially available for Fj hybrids.

Due to the prominent role of inbred lineages in the production of commercial Fj maize hybrids, the development of inbred lineages has received particular emphasis in the field of maize cultivation and hybridization. Thus, it has become necessary to develop new inbred lines that produce first generation hybrid maize with higher yields, greater stability, and greater resistance during hybridization. Another requirement is the homogeneity of the first generation hybrids, which facilitates mechanical harvesting and reduces labor requirements.

As a result, there are concerted efforts to develop inbred lines for the production of commercially available first generation maize hybrids.

The present invention relates to the production of an inbred line of the genus DE 0719, the main characteristic of which is that when crossed with another inbred maize as a female or male parent unit, first generation (Fj) hybrids with advantageous characteristics are obtained.

Thus, in accordance with the present invention, an inbred maize line of the variety DE 0719 is used which has high yield, excellent stalk and excellent phenotypic properties.

The inbred maize line DE 0719 of the present invention can be used to produce hybrid maize, wherein a first maize line is crossed with a second maize line, wherein a first maize line or a second maize line is a DE maize inbred or a second maize line.

The strain DE 0719 of the present invention, when used as a lineage, can be crossed with other maize lines, preferably inbred maize lines, to form first generation (Fj) maize hybrids. Although the inbred line DE 0719 is generally used as a female during crossing, it can also be used as a male.

The inbred maize line DE 0719 is a yellow horse-bred inbred line and its breeding is described below:

The inbred maize line DE 0719 of horse origin originates from the crossing of lines 5B2C-A and DE 0661. To produce line 5B2C-A, line B 73 was crossed with line CM 105 and then crossed back with line B 73. Both parent lines are the property of DEKALB Plant Genetics. The original crossing was done by Dr. PFUND John in Dekalb (Illinois, USA) at lines 26,628 and 26,629, the following breeding! In the description, summer operations were carried out in the above horticulture and winter operations were carried out in Kaunai, Hawaii, USA.

In the summer of 1982, we crossed 5B2C-A (B 73 ”CM105) x PB 80 (Β 73” 1067-1) at lines 26,628 and 26,629.

In the winter of 1982, the accumulated S0 * seeds were grown and self-pollinated. All Si seeds were harvested and accumulated (lines 5-923 and 5-924).

* S represents the level of inbreeding.

HU 210 974 B

In the summer of 1983, the accumulated SI seeds were grown in separate rows per tube. 30 self-pollinated tubes were selected from the 40 rows and stored separately (rows 5272-5311).

In the summer of 1984, 30 S3 tubes were planted in separate rows, 35 S3 tubes were sorted and stored separately (lines 7541-7570).

In the summer of 1985, 35 S3 tubes were planted in separate rows and 24 S4 tubes were selected (lines 9017-9051).

In the winter of 1986, 24 S4 tubes were removed and a single S5 tube was selected from lines 5-1281 and 5-1282. It got the FBLL code.

The FBLL line was designated DE 0719, and its pure source was maintained by self-pollination and the accumulation of selected tubes.

The line DE 0719 was stable during self-pollinating maintenance and was distinguishable and uniform with respect to the properties tested. DE 0719 does not show more variety than would be expected from the environment.

The inbred line DE 0719 is the property of Dekalb Plant Genetics and was not accessible to users.

Cells of the inbred line of DE 0719, which can be cultured in a suitable culture, then separated and regenerated to form the inbred line, are within the scope of the present invention. Details of the generation process are described in C.E. Green and C.A. Rhodes, Plant Regeneraton in Tissue Culture of Maize, Maize for Biology Research, W.F. Sheridan Publisher, Plant Molecular Biology Association, Charlottesville, USA, 367-372 (1982).

As mentioned above, the inbred line DE 0719 is a yellow horse-toothed corn which is preferably adapted for cultivation in the northern corn zone of the USA.

This inbred line has the following characteristics (UPOV: Guidelines for Conduct of Tests for Distinctness, Homogeneity and Stability for Maize, 12 November 1980):

Letter, look

Stems, lumps anthocyanin coloring-

(in the center of the plant): weak Letter, envelope anthocyanin discoloration (in the center of the plant): weak Letter, fluffiness: strong Leaf, hairs on edge of envelope: strong Coat of arms, crankshaft and side angles (in the lower third of the coat of arms): small Coat of arms, appearance of side branches: slightly sloping Coat of arms, number of primary side branches: few Coat of arms, crankshaft length is the lowest above the side: very short Coat of arms, crankshaft length a above the highest branch: short Plant height (including tassel): long Pipe, where the pipe fits into the female relative to total prescription height: tall Pipe, handle length: short Pipe, length: medium Tube diameter (center of tube): medium Tube Shape: Slightly conical Pipe, number of cores: many Pipe, type of cores (in the middle third of the tube): dent Tube, core tip color: yellow Tube, core backside color: orange Tube, the chaff anthocyanin discoloration: there is Tube, the chaff anthocyanin intensity of coloration: medium

The inbred maize line DE 0719 is a yellow inbred inbred line derived from the intersection of lines 5B2C-A x DE 0661. The closest known maize line is DE 0661. The inbred line DE 0719 differs significantly from the known inbred line DE 0661 in plant height (191.2 cm and 227.3 cm) and tube height (73.9 cm and

99.6 cm), anthocyanin coloration of the stem (weak or strong), color of the leaf after pollination (medium green or dark green), length of the leaf (70.9 cm and 78.8 cm), width of the leaf (17, 1 cm and 8.9, respectively, in the anthocyanin coloration of the leaf envelope (strong or medium), in the length of the coat of arms (22.2 cm and

30.6 cm), the length of the handle (10.2 cm and 13.1 cm), the color of the anthers (pink and greenish yellow) and the strength of the cob (weak or strong).

The above characteristics of the inbred line DE 0719 relative to the known inbred line DE 0661 are shown in Table 1:

(middle third of plant): Slightly sloping crest, beginning of flowering: late

Coat of Arms, Ports anthocyanin

discoloration: you are missing Tube, mustache appearance (the plants very weak 50%): Tube, mustache anthocyanin late discoloration: Coat of arms, chaff anthocyanin missing or very weak discoloration: Coat of arms, anthocyanic ring none or very weak at the base of the chaff: Stems, knots anthocyanin no discoloration: none or very weak

Table 1

specific DE 0719 DE 0661 THE B plant height (cm) 191.2 227.3 57.305 15.0 tube height (cm) 73.9 - 99.6 anthocyanin coloration of the stem weak strong

HU 210 974 Β

specific DE 0719 DE 0661 THE B color after pollination medium green dark green leaf length (cm) 70.9 78.8 6.401 5.0 leaf width (cm) 17.1 8.9 0.348 1.2 anthocyanin coloration of the envelope weak strong envelope fluffiness strong medium crest length (cm) 22.2 30.6 handle length (cm) 10.2 13.1 color of ports pink greenish yellow cob strength weak strong

A: squared error average

B: standard deviation, significance = 0.05

Fig. 1 shows the female inflorescence of the maize line DE 0719, Fig. 2 shows the sense of support and Fig. 3 the male inflorescence. Figures 4 and 5 show a control inbred line of DE 0661.

An isozyme analysis of the inbred lines DE 0719 and DE 0661 shows that there is a genetic difference in one place:

Acphl = 2 instead of 2/2, 2/4 and 4/4.

The isozyme genotype of the inbred line DE 0719 compared to the known inbred line DE 0661 is shown in Table 2.

Technique for determination of isozyme genotype by Goodman man. N. and Stuber C. W., Genetic Identification of Lines and Crosses Using Isoenzyme Electrophoresis, Proceedings of the Thirty-Fifth Annual Com and Sorghum Industries Research Conference, 1980.

Table 2

Allelomorph presence Place DE 0719 DE 0661 Acphl 2 * adhl 4 4 Cat3 9 9 Gdhl 1 1 Got3 4 4 Got2 4 4 Gotl 4 4 Glu B 7 7 Idhl 4 4 Idh2 4 4 Mdhl 6 6 Mdh2 3.5 3.5 Mdh3 16 16

Allelomorph presence Place DE 0719 DE 0661 Mdh4 12 12 Mdh5 12 12 pgml 9 9 Pgm2 4 4 6 Pgdl 3.8 3.8 6 Pgd2 5 5 Phi 4 4 number of plants tested 6 6

* can be 2/2, 2 / 4.4 / 4

The inbred line DE 0719 is preferably used as a parent line for the production of hybrid maize, in particular first generation (Fj) hybrid maize. When used, this inbred line is used in combination with another inbred line and the two lines are hybridized by conventional techniques. That is, the two inbred lines, one of which is the inbred line DE 0719, are planted in close proximity to each other for pollination. This can be done by placing the parent lines in alternating rows, blocks, or any other way. The plants of the two lines are grown until flowering. Preferably, during the development period, the plants are regularly thinned in the triple leaf state and regularly treated with fertilizers and / or agrochemicals for proper development.

During flowering, when the inbred line DE 0719 is used as a female parent, the coat of arms of the parent DE 0719 is removed from all plants. This removal can be done manually or mechanically.

Female parental units can also be chemically sterilized by the simultaneous incorporation of a cytoplasmic male sterile property.

The lines are further cultivated, while the grasses normally cross-pollinate due to the wind. As a result of the neutering of the female lineage, all pollen from the male lineage is suitable for pollination since all the plants of the inbred lineage, and thus the pollen-bearing part of the female, which have played a female role in hybridization have been removed. During the hybridization process, the parental units are reared separately from corn fields to avoid undesirable introduction of pollen from foreign varieties. The necessary isolation techniques are well known to those skilled in the art.

Thereafter, both parent lines are further cultivated until maturity, but only tubes from the female parent units are harvested to harvest the seeds of the new Fj hybrid maize. Seeds from the male parent unit may also be harvested and used as needed, but these seeds may not be used as hybrid seeds.

New F] hybrid seeds obtained next season

HU 210 974 B can be planted when high yielding F] hybrid maize plants develop.

The inbred line DE 0719 is suitable for the production of new maize plants Fj which have advantageous properties, for example, the inbred line of the DE 0719 variety is used as the female parent line and the DE 0726 inbred line is used as the male parent line. The first generation (Fj) hybrid received the DK 554 variety mark.

The characteristics of the first generation (Fi) DK 554 hybrid were compared to those of the commercially available Pioneer 3475 and Volga. Both variants have similar yields and are particularly suitable for comparison. The results are shown in Table 3.

Table 3 DK554 and P Volga

Hybrid DK 554 Volga Signifikancia crop (bush * / ha) 143.6 136.9 XX content of core moisture (%) 15.9 17.4 XX unit of growth until flowering 1271 1269 plant height (cm) 197.4 199.8 + tube height (cm) 93.2 92.2 barrel tube (%) 1.8 3.5 XX dead tube (%) 0.1 0.5 XX stem inclination (%) 4.1 2.8 XX rooting (%) 1.4 3.4 XX

number of seats: 168 x statistically significant at 5% xx statistically significant at 1% + statistically significant at 10% * 1 bus = 36.35 liters

It can be seen from the foregoing that the inbred line DE 0719 provides a preferred breeding strain when used as a male or female lineage for the production of first generation hybrids with particular and advantageous properties.

Claims (1)

Patent Claim Point Inbred maize line DE 0719 obtained by crossing lines 5B2C-A x DE 0661 with slight leaf appearance, late onset of flowering, late appearance of mustache, slight anthocyanin coloration of the mustache, anthrax, closed anthocyanin ring missing at the base of the chaff, slight anthocyanin coloration of nodules, weak anthocyanin coloration of nodules, small angle between crankshaft and side branches, slight appearance of lateral branches, very small number of primary side branches, , the crankshaft length above the uppermost branch is short, the tube is medium long, the diameter is medium and slightly conical, the number of nuclei is large, the nucleus is equine, the nucleus is yellow, the backside is orange and the anthocyanin coloration is medium, Nove ny high, tube high in height, with little or no anthocyanin coloration of the stem, medium green after pollination, length 68-73 cm, preferably 71 cm, width 15-18 cm, preferably 17 cm, weak anthocyanin coloration of the sheath It has a strong pile, a tassel length of 20-24 cm, preferably 22 cm, a tube handle of 8-12 cm, preferably 10 cm, anthocyanin coloration of the anthers and a weak cob strength. HU 210 974 B