HRP20211939T1 - Varijanta oleaginoznog kvasca i njegova primjena za proizvodnju lipida - Google Patents
Varijanta oleaginoznog kvasca i njegova primjena za proizvodnju lipida Download PDFInfo
- Publication number
- HRP20211939T1 HRP20211939T1 HRP20211939TT HRP20211939T HRP20211939T1 HR P20211939 T1 HRP20211939 T1 HR P20211939T1 HR P20211939T T HRP20211939T T HR P20211939TT HR P20211939 T HRP20211939 T HR P20211939T HR P20211939 T1 HRP20211939 T1 HR P20211939T1
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- Croatia
- Prior art keywords
- hours
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- carried out
- production
- lipids
- Prior art date
Links
- 150000002632 lipids Chemical class 0.000 title claims 10
- 238000004519 manufacturing process Methods 0.000 title claims 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims 4
- 238000000034 method Methods 0.000 claims 13
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims 6
- 241000439394 Cutaneotrichosporon oleaginosum Species 0.000 claims 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims 3
- 239000008103 glucose Substances 0.000 claims 3
- 239000001963 growth medium Substances 0.000 claims 3
- 239000002054 inoculum Substances 0.000 claims 3
- 229910052757 nitrogen Inorganic materials 0.000 claims 3
- 238000011081 inoculation Methods 0.000 claims 2
- 239000002028 Biomass Substances 0.000 claims 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims 1
- 240000008042 Zea mays Species 0.000 claims 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims 1
- 239000008346 aqueous phase Substances 0.000 claims 1
- 239000007900 aqueous suspension Substances 0.000 claims 1
- 229940041514 candida albicans extract Drugs 0.000 claims 1
- 239000004202 carbamide Substances 0.000 claims 1
- 229910052799 carbon Inorganic materials 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 235000005822 corn Nutrition 0.000 claims 1
- 239000000284 extract Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 230000003834 intracellular effect Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 210000005253 yeast cell Anatomy 0.000 claims 1
- 239000012138 yeast extract Substances 0.000 claims 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (8)
1. Oleaginozni kvasac vrste Trichosporon oleaginosus podnijet 17. svibnja 2017. prema Budimpeštanskom sporazumu Leibniz-Institutu DSMZ - Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH - Inhoffenstraße 7 B 38124 Braunschweig (Njemačka), depozit broj DSM 32508.
2. Proces za proizvodnju lipida koji obuhvaća:
- pripremu inokuluma koji sadrži najmanje oleaginozni kvasac vrste Trichosporon oleaginosus DSM 32508 prema patentnom zahtjevu 1;
- dopremanje spomenutog inokuluma u uređaj za kultivaciju da se dobije bujon kulture;
- podvrgavanje spomenutog bujona kulture razdvajanju da se dobije vodena suspenzija koncentrirane oleaginozne stanične biomase koja sadrži lipide i vodenu fazu;
- ekstrakciju unutarstaničnih lipida akumuliranih unutar stanica kvasca.
3. Proces za proizvodnju lipida prema patentnom zahtjevu 2, pri čemu navedeni proces:
- se provodi na temperaturi u opsegu od 10°C do 40°C, poželjno u opsegu od 20°C do 35°C; i/ili
- se provodi tokom vremena u opsegu od 40 sati do 200 sati, poželjno u opsegu od 80 sati do 150 sati; i/ili
- se provodi pod aerobnim uvjetima; i/ili
- se provodi pri pH u opsegu od 4,5 do 7,0 poželjno u opsegu od 5,0 do 6,5.
4. Proces za proizvodnju lipida prema patentnom zahtjevu 2 ili 3, pri čemu se navedeni proces provodi polazeći od inokuluma u količini u opsegu od 1% do 5% (vol/vol) ukupne zapremine medija kulture, dobivenom iz prethodne kulture navedenog oleaginoznog kvasca vrste Trichosporon oleaginosus DSM 32508, provedene u istom mediju kulture tokom vremena u opsegu od 6 sati do 24 sata.
5. Proces za proizvodnju lipida prema bilo kojem od patentnih zahtjeva od 2 do 4, pri čemu se navedeni proces provodi u mediju kulture koji sadrži glukozu kao izvor ugljika i amonij sulfata [(NH4)2SO4] kao izvor dušika.
6. Proces za proizvodnju lipida prema bilo kojem od patentnih zahtjeva od 2 do 5, pri čemu se navedeni proces provodi u napajanoj šarži.
7. Proces za proizvodnju lipida prema bilo kojem od patentnih zahtjeva od 2 do 5, pri čemu se navedeni proces, nakon vremena u opsegu od 15 sati do 25 sati nakon inokulacije, dalje provodi u napajanoj šarži, poželjno tokom vremena u opsegu od 25 sati do 175 sati, poželjnije u opsegu od 65 sati do 125 sati, dodavanjem najmanje dodatnog izvora dušika kao što je koncentrirani kukuruzni ekstrakt, ekstrakt kvasca, amonij sulfat, urea, u takvoj količini kao da se dodaje količina dušika u opsegu od 0,5 g/L do 5 g/L, u bujon kulture.
8. Proces za proizvodnju lipida prema bilo kojem od patentnih zahtjeva od 2 do 7, pri čemu se navedeni proces, nakon vremena u opsegu od 15 sati do 25 sati nakon inokulacije, dalje provodi u napajanoj šarži, poželjno tokom vremena u opsegu od 25 sati do 175 sati, poželjnije u opsegu od 65 sati do 125 sati, dodavanjem vodene otopine glukoze tako da je koncentracija glukoze konstantna u bujonu kulture u opsegu od 25 g/L do 50 g/L.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT102017000081383A IT201700081383A1 (it) | 2017-07-18 | 2017-07-18 | Variante di lievito oleaginoso e suo utilizzo per la produzione di lipidi. |
| EP18755320.1A EP3655521B1 (en) | 2017-07-18 | 2018-07-17 | Variant of oleaginous yeast and its use for the production of lipids |
| PCT/IB2018/055301 WO2019016703A1 (en) | 2017-07-18 | 2018-07-17 | VARIANT OF OLEAGINOUS YEAST AND ITS USE FOR THE PRODUCTION OF LIPIDS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| HRP20211939T1 true HRP20211939T1 (hr) | 2022-03-18 |
Family
ID=60990908
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| HRP20211939TT HRP20211939T1 (hr) | 2017-07-18 | 2018-07-17 | Varijanta oleaginoznog kvasca i njegova primjena za proizvodnju lipida |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US11326192B2 (hr) |
| EP (1) | EP3655521B1 (hr) |
| CN (1) | CN111655838B (hr) |
| BR (1) | BR112020000853A2 (hr) |
| DK (1) | DK3655521T3 (hr) |
| ES (1) | ES2900031T3 (hr) |
| HR (1) | HRP20211939T1 (hr) |
| HU (1) | HUE056868T2 (hr) |
| IT (1) | IT201700081383A1 (hr) |
| MY (1) | MY193244A (hr) |
| PL (1) | PL3655521T3 (hr) |
| RS (1) | RS62716B1 (hr) |
| UA (1) | UA127184C2 (hr) |
| WO (1) | WO2019016703A1 (hr) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111690587B (zh) * | 2019-03-13 | 2022-10-25 | 上海凯赛生物技术股份有限公司 | 一种离心筛选具有高含油率油脂酵母菌株的方法及其应用 |
| EP4204540A1 (en) * | 2020-08-26 | 2023-07-05 | ENI S.p.A. | Oleaginous yeast strain and use thereof for the production of lipids |
| CN112391421A (zh) * | 2020-11-19 | 2021-02-23 | 中国科学院青岛生物能源与过程研究所 | 一种通过调节酿酒酵母发酵液总盐含量提高酿酒酵母油脂含量的培养方法及高产油酿酒酵母 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434912B (zh) * | 2008-12-12 | 2011-01-19 | 华东理工大学 | 一种产油酵母菌株及其制备生物油脂的方法 |
| CN101955888B (zh) * | 2010-06-25 | 2012-09-26 | 朱笃 | 高产油脂皮状丝孢酵母b3及其ems和紫外线复合诱变选育方法 |
| ITUB20152958A1 (it) * | 2015-08-06 | 2017-02-06 | Eni Spa | Metodo per concentrare una sospensione cellulare comprendente una biomassa mucillaginosa di lieviti oleaginosi. |
-
2017
- 2017-07-18 IT IT102017000081383A patent/IT201700081383A1/it unknown
-
2018
- 2018-07-17 UA UAA202000379A patent/UA127184C2/uk unknown
- 2018-07-17 MY MYPI2020000223A patent/MY193244A/en unknown
- 2018-07-17 RS RS20211545A patent/RS62716B1/sr unknown
- 2018-07-17 WO PCT/IB2018/055301 patent/WO2019016703A1/en unknown
- 2018-07-17 HU HUE18755320A patent/HUE056868T2/hu unknown
- 2018-07-17 ES ES18755320T patent/ES2900031T3/es active Active
- 2018-07-17 PL PL18755320T patent/PL3655521T3/pl unknown
- 2018-07-17 CN CN201880047838.2A patent/CN111655838B/zh active Active
- 2018-07-17 BR BR112020000853-2A patent/BR112020000853A2/pt unknown
- 2018-07-17 EP EP18755320.1A patent/EP3655521B1/en active Active
- 2018-07-17 DK DK18755320.1T patent/DK3655521T3/da active
- 2018-07-17 US US16/631,188 patent/US11326192B2/en active Active
- 2018-07-17 HR HRP20211939TT patent/HRP20211939T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PL3655521T3 (pl) | 2022-02-07 |
| CN111655838A (zh) | 2020-09-11 |
| BR112020000853A2 (pt) | 2020-07-21 |
| MY193244A (en) | 2022-09-27 |
| HUE056868T2 (hu) | 2022-03-28 |
| US20200149076A1 (en) | 2020-05-14 |
| IT201700081383A1 (it) | 2019-01-18 |
| RS62716B1 (sr) | 2022-01-31 |
| EP3655521B1 (en) | 2021-11-03 |
| EP3655521A1 (en) | 2020-05-27 |
| ES2900031T3 (es) | 2022-03-15 |
| UA127184C2 (uk) | 2023-05-31 |
| US11326192B2 (en) | 2022-05-10 |
| WO2019016703A1 (en) | 2019-01-24 |
| DK3655521T3 (da) | 2021-12-13 |
| CN111655838B (zh) | 2023-05-02 |
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